(Linear) (Six-base) MAPSORT of: KIEE5814.seq Check: 46 from: 1 to: 2739 KIEE5814.orf With 182 enzymes: SgfI * December 5, 2014 13:12 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1641 1651 2739 Size: 1641 10 1088 Fragments arranged by size: 1641 1088 10 AatII G_ACGT'C Cuts at: 0 2291 2739 Size: 2291 448 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 409 1301 1841 2739 Size: 409 892 540 898 Fragments arranged by size: 898 892 540 409 AflII C'TTAA_G Cuts at: 0 1573 2739 Size: 1573 1166 AflIII A'CryG_T Cuts at: 0 1241 2739 Size: 1241 1498 AgeI A'CCGG_T Cuts at: 0 2545 2739 Size: 2545 194 AhdI GACnn_n'nnGTC Cuts at: 0 1815 2258 2739 Size: 1815 443 481 Fragments arranged by size: 1815 481 443 AleI CACnn'nnGTG Cuts at: 0 185 2525 2739 Size: 185 2340 214 Fragments arranged by size: 2340 214 185 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 630 2189 2739 Size: 630 1559 550 Fragments arranged by size: 1559 630 550 ApaI G_GGCC'C Cuts at: 0 2159 2739 Size: 2159 580 ApaLI G'TGCA_C Cuts at: 0 172 2174 2739 Size: 172 2002 565 Fragments arranged by size: 2002 565 172 ApoI r'AATT_y Cuts at: 0 779 1558 2007 2111 2467 2739 Size: 779 779 449 104 356 272 Fragments arranged by size: 779 779 449 356 272 104 AseI AT'TA_AT Cuts at: 0 35 2087 2739 Size: 35 2052 652 Fragments arranged by size: 2052 652 35 AvaI C'yCGr_G Cuts at: 0 2054 2354 2739 Size: 2054 300 385 Fragments arranged by size: 2054 385 300 BamHI G'GATC_C Cuts at: 0 1894 2730 2739 Size: 1894 836 9 Fragments arranged by size: 1894 836 9 BanII G_rGCy'C Cuts at: 0 985 1255 1465 2159 2510 2739 Size: 985 270 210 694 351 229 Fragments arranged by size: 985 694 351 270 229 210 BclI T'GATC_A Cuts at: 0 1419 1778 2739 Size: 1419 359 961 Fragments arranged by size: 1419 961 359 Bme1580I G_kGCm'C Cuts at: 0 176 2159 2178 2444 2739 Size: 176 1983 19 266 295 Fragments arranged by size: 1983 295 266 176 19 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1773 2739 Size: 1773 966 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1376 2395 2739 Size: 1376 1019 344 Fragments arranged by size: 1376 1019 344 Bpu10I CC'TnA_GC Cuts at: 0 1652 2739 Size: 1652 1087 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1250 2739 Size: 1250 1489 BsaI GGTCTCn'nnnn_ Cuts at: 0 2492 2627 2739 Size: 2492 135 112 Fragments arranged by size: 2492 135 112 BsaAI yAC'GTr Cuts at: 0 16 1244 2100 2739 Size: 16 1228 856 639 Fragments arranged by size: 1228 856 639 16 BsaBI GATnn'nnATC Cuts at: 0 1071 1534 1732 2739 Size: 1071 463 198 1007 Fragments arranged by size: 1071 1007 463 198 BsaHI Gr'CG_yC Cuts at: 0 1475 2288 2739 Size: 1475 813 451 Fragments arranged by size: 1475 813 451 BsaWI w'CCGG_w Cuts at: 0 1869 2545 2739 Size: 1869 676 194 Fragments arranged by size: 1869 676 194 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1595 1800 2442 2739 Size: 1595 205 642 297 Fragments arranged by size: 1595 642 297 205 BseYI C'CCAG_C Cuts at: 0 1053 2739 Size: 1053 1686 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 646 664 2319 2674 2739 Size: 646 18 1655 355 65 Fragments arranged by size: 1655 646 355 65 18 BsiHKAI G_wGCw'C Cuts at: 0 176 911 1085 2178 2739 Size: 176 735 174 1093 561 Fragments arranged by size: 1093 735 561 176 174 BsmI GAATG_Cn' Cuts at: 0 1646 2739 Size: 1646 1093 Bsp1286I G_dGCh'C Cuts at: 0 176 911 985 1085 1255 1465 2159 2178 Size: 176 735 74 100 170 210 694 19 Cuts at: 2178 2444 2510 2739 Size: 266 66 229 Fragments arranged by size: 735 694 266 229 210 176 170 100 74 66 19 BspEI T'CCGG_A Cuts at: 0 1869 2739 Size: 1869 870 BspHI T'CATG_A Cuts at: 0 2291 2739 Size: 2291 448 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 427 1641 1651 2178 2603 2739 Size: 427 1214 10 527 425 136 Fragments arranged by size: 1214 527 427 425 136 10 BsrBI CCG'CTC Cuts at: 0 1799 2739 Size: 1799 940 BsrFI r'CCGG_y Cuts at: 0 2545 2739 Size: 2545 194 BssHII G'CGCG_C Cuts at: 0 2640 2739 Size: 2640 99 BstEII G'GTnAC_C Cuts at: 0 1449 2739 Size: 1449 1290 BstYI r'GATC_y Cuts at: 0 675 1066 1894 2277 2730 2739 Size: 675 391 828 383 453 9 Fragments arranged by size: 828 675 453 391 383 9 Bsu36I CC'TnA_GG Cuts at: 0 944 2739 Size: 944 1795 BtgI C'CryG_G Cuts at: 0 233 713 1898 2298 2433 2739 Size: 233 480 1185 400 135 306 Fragments arranged by size: 1185 480 400 306 233 135 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2127 2325 2739 Size: 2127 198 414 Fragments arranged by size: 2127 414 198 BtsI GCAGTG_nn' Cuts at: 0 346 443 2520 2532 2739 Size: 346 97 2077 12 207 Fragments arranged by size: 2077 346 207 97 12 DraI TTT'AAA Cuts at: 0 129 2739 Size: 129 2610 DraIII CAC_nnn'GTG Cuts at: 0 1449 2400 2439 2739 Size: 1449 951 39 300 Fragments arranged by size: 1449 951 300 39 DrdI GACnn_nn'nnGTC Cuts at: 0 1356 2739 Size: 1356 1383 EaeI y'GGCC_r Cuts at: 0 150 1689 1979 2272 2739 Size: 150 1539 290 293 467 Fragments arranged by size: 1539 467 293 290 150 EarI CTCTTCn'nnn_ Cuts at: 0 1185 2739 Size: 1185 1554 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 2265 2739 Size: 2265 474 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 409 1301 1376 1841 2395 2739 Size: 409 892 75 465 554 344 Fragments arranged by size: 892 554 465 409 344 75 EcoO109I rG'GnC_Cy Cuts at: 0 1917 2155 2156 2739 Size: 1917 238 1 583 Fragments arranged by size: 1917 583 238 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2710 2739 Size: 2710 29 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 68 100 214 246 1303 1335 1801 1833 Size: 68 32 114 32 1057 32 466 32 Cuts at: 1833 2244 2276 2437 2469 2739 Size: 411 32 161 32 270 Fragments arranged by size: 1057 466 411 270 161 114 68 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 416 2228 2739 Size: 416 1812 511 Fragments arranged by size: 1812 511 416 HpaI GTT'AAC Cuts at: 0 416 2739 Size: 416 2323 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2480 2739 Size: 2480 259 MscI TGG'CCA Cuts at: 0 152 1981 2274 2739 Size: 152 1829 293 465 Fragments arranged by size: 1829 465 293 152 MslI CAynn'nnrTG Cuts at: 0 185 1712 2171 2221 2525 2650 2739 Size: 185 1527 459 50 304 125 89 Fragments arranged by size: 1527 459 304 185 125 89 50 MspA1I CmG'CkG Cuts at: 0 715 2739 Size: 715 2024 NcoI C'CATG_G Cuts at: 0 1898 2739 Size: 1898 841 NspI r_CATG'y Cuts at: 0 864 2739 Size: 864 1875 PflMI CCAn_nnn'nTGG Cuts at: 0 2727 2739 Size: 2727 12 PspOMI G'GGCC_C Cuts at: 0 2155 2739 Size: 2155 584 PstI C_TGCA'G Cuts at: 0 353 1188 2527 2739 Size: 353 835 1339 212 Fragments arranged by size: 1339 835 353 212 SacII CC_GC'GG Cuts at: 0 716 2739 Size: 716 2023 ScaI AGT'ACT Cuts at: 0 970 1486 2269 2739 Size: 970 516 783 470 Fragments arranged by size: 970 783 516 470 SfcI C'TryA_G Cuts at: 0 349 1184 1959 2523 2739 Size: 349 835 775 564 216 Fragments arranged by size: 835 775 564 349 216 SmaI CCC'GGG Cuts at: 0 2056 2739 Size: 2056 683 SmlI C'TyrA_G Cuts at: 0 1265 1573 2739 Size: 1265 308 1166 Fragments arranged by size: 1265 1166 308 SspI AAT'ATT Cuts at: 0 205 1018 2739 Size: 205 813 1721 Fragments arranged by size: 1721 813 205 StyI C'CwwG_G Cuts at: 0 760 1898 2739 Size: 760 1138 841 Fragments arranged by size: 1138 841 760 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 13 175 753 2392 2739 Size: 13 162 578 1639 347 Fragments arranged by size: 1639 578 347 162 13 TatI w'GTAC_w Cuts at: 0 968 1484 2138 2267 2669 2739 Size: 968 516 654 129 402 70 Fragments arranged by size: 968 654 516 402 129 70 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 885 917 1632 1664 2739 Size: 885 32 715 32 1075 Fragments arranged by size: 1075 885 715 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 106 2607 2739 Size: 106 2501 132 Fragments arranged by size: 2501 132 106 XmaI C'CCGG_G Cuts at: 0 2054 2739 Size: 2054 685 XmnI GAAnn'nnTTC Cuts at: 0 708 799 2690 2739 Size: 708 91 1891 49 Fragments arranged by size: 1891 708 91 49 ZraI GAC'GTC Cuts at: 0 2289 2739 Size: 2289 450 Enzymes that do cut: AarI AatII AcuI AflII AflIII AgeI AhdI AleI AlfI ApaI ApaLI ApoI AseI AvaI BamHI BanII BclI Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BssHII BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EarI EciI Eco57MI EcoO109I FalI Hin4I HincII HpaI MmeI MscI MslI MspA1I NcoI NspI PflMI PspOMI PstI SacII ScaI SfcI SmaI SmlI SspI StyI TaqII TatI TstI XcmI XmaI XmnI ZraI Enzymes that do not cut: AccI Acc65I AclI AfeI AloI AlwNI AscI AsiSI AvrII BaeI BanI BbeI BbsI BbvCI BcgI BciVI BfrBI BglI BglII BlpI BmgBI BmrI BmtI BsaXI BsiEI BsiWI BsmBI BsrDI BsrGI BssSI BstAPI BstBI BstXI BstZ17I ClaI CspCI EagI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HindIII KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI StuI SwaI Tth111I XbaI XhoI