Restriction map for
pFN21AE5320
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# NO CUT ENZYMES ( 162 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AflIII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I AspEI AspHI AspI
AvaII AviII AvrII BamHI BbeI
BbrPI BbsI BbvCI BcgI BclI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DpnII
DraI DraII DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI KasI Ksp632I KspI MamI
MboI MfeI MflI MluI MroI
MunI NaeI NarI NdeI NdeII
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SapI Sau3AI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI Tth111I
TthHB8I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 463 GGTACC G^GTACC
AccI 197 GTCTAC GT^[AC][GT]AC
AccII 69 CGCG CG^CG
AfaI 464 GTAC GT^AC
AlwNI 595 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 446 GGGCCC GGGCC^C
Asp718I 463 GGTACC G^GTACC
AvaI 449 CCCGAG C^[CT]CG[GA]G
BalI 650 TGGCCA TGG^CCA
BanI 463 GGTACC G^G[CT][GA]CC
BanII 446 GGGCCC G[GA]GC[CT]^C
BciVI 711 GGATAC GTATCC(6/5)
BcnI 109 CCGGG CC^[GC]GG
BmyI 446 GGGCCC G[AGT]GC[ACT]^C
BsgI 455 GTGCAG GTGCAG(16/14)
BsmFI 14 GGGAC GGGAC(10/14)
BsoBI 449 CCCGAG C^[CT]CG[GA]G
Bsp1286I 446 GGGCCC G[AGT]GC[ACT]^C
BspHI 428 TCATGA T^CATGA
BspMI 457 GCAGGT ACCTGC(4/8)
BsrFI 819 GCCGGT [GA]^CCGG[CT]
BstEII 552 GGTAACC G^GT[ACGT]ACC
BstNI 306 CCAGG CC^[AT]GG
BstPI 552 GGTAACC G^GT[ACGT]ACC
BstUI 69 CGCG CG^CG
BstXI 168 CCACTGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 70 GCGC GCG^C
Cfr10I 819 GCCGGT [GA]^CCGG[CT]
DsaI 10 CCATGG C^C[GA][CT]GG
EaeI 650 TGGCCA [CT]^GGCC[GA]
EcoO65I 552 GGTAACC G^GT[ACGT]ACC
EcoRII 306 CCAGG ^CC[AT]GG
HhaI 70 GCGC GCG^C
HinP1I 70 GCGC G^CGC
KpnI 463 GGTACC GGTAC^C
MluNI 650 TGGCCA TGG^CCA
MscI 650 TGGCCA TGG^CCA
MseI 845 TTAA T^TAA
MspA1I 116 CAGCTG C[AC]G^C[GT]G
MvaI 306 CCAGG CC^[AT]GG
MvnI 69 CGCG CG^CG
NciI 109 CCGGG CC^[GC]GG
NcoI 10 CCATGG C^CATGG
NspI 393 ACATGC [GA]CATG^[CT]
PleI 386 GAGTC GAGTC(4/5)
PvuII 116 CAGCTG CAG^CTG
RcaI 428 TCATGA T^CATGA
RsaI 464 GTAC GT^AC
Tru9I 845 TTAA T^TAA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 185 GAATTT [GA]^AATT[CT]
477 GAATTT
ApoI 185 GAATTT [GA]^AATT[CT]
477 GAATTT
BfaI 79 CTAG C^TAG
298 CTAG
BsrI 252 ACTGG ACTGG(1/-1)
336 ACTGG
BssKI 109 CCGGG ^CC[ACGT]GG
306 CCAGG
BstF5I 442 GGATG GGATG(2/0)
725 GGATG
Cfr13I 446 GGGCC G^G[ACGT]CC
447 GGCCC
DdeI 834 CTCAG C^T[ACGT]AG
860 CTAAG
EcoT14I 10 CCATGG C^C[AT][AT]GG
548 CCAAGG
FauI 38 CCCGC CCCGC(4/6)
288 CCCGC
FokI 442 GGATG GGATG(9/13)
725 GGATG
HapII 109 CCGG C^CGG
820 CCGG
HpaII 109 CCGG C^CGG
820 CCGG
MaeI 79 CTAG C^TAG
298 CTAG
MaeII 43 ACGT A^CGT
102 ACGT
MslI 42 CACGTCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
122 CATTGGCATG
MspI 109 CCGG C^CGG
820 CCGG
NlaIV 446 GGGCCC GG[ACGT]^[ACGT]CC
463 GGTACC
PflMI 362 CCAGAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
594 CCAGCATCTGG
Sau96I 446 GGGCC G^G[ACGT]CC
447 GGCCC
ScrFI 109 CCGGG CC^[ACGT]GG
306 CCAGG
StyI 10 CCATGG C^C[AT][AT]GG
548 CCAAGG
TfiI 4 GATTC G^A[AT]TC
135 GAATC
Tsp509I 186 AATT ^AATT
478 AATT
TspRI 86 CAGTG CA[GC]TG[ACGT][ACGT]^
169 CACTG
Van91I 362 CCAGAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
594 CCAGCATCTGG
XcmI 168 CCACTGTTTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
638 CCATCTTTATTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AciI AluI BbvI BsaJI BsiYI
BslI Cac8I Fnu4HI HaeIII HinfI
HphI ItaI MaeIII MboII MnlI
MwoI NlaIII SfaNI TseI Tsp45I