Restriction map for
pFN21AE6635
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII AccI AccII AccIII AciI
AclI AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI AspEI AspI
AviII AvrII BanII BbrPI BbsI
BbvCI BcgI BclI BcnI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BseRI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstF5I BstPI BstUI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV FauI FbaI FokI
FseI FspI HapII HindIII HpaI
HpaII Ksp632I KspI MaeII MaeIII
MamI MfeI MluI MroI MspI
MunI MvnI NaeI NciI NdeI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfcI SfiI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SspBI
StuI SwaI Tsp45I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 393 GGTACC G^GTACC
AcyI 51 GGCGCC G[GA]^CG[CT]C
AflII 130 CTTAAG C^TTAAG
Asp700I 147 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 393 GGTACC G^GTACC
AvaI 26 CTCGGG C^[CT]CG[GA]G
AvaII 82 GGACC G^G[AT]CC
BamHI 645 GGATCC G^GATCC
BbeI 51 GGCGCC GGCGC^C
BciVI 211 GGATAC GTATCC(6/5)
BfrI 130 CTTAAG C^TTAAG
BpmI 216 CTGGAG CTGGAG(16/14)
BsaHI 51 GGCGCC G[GA]^CG[CT]C
BsmAI 627 GAGAC GTCTC(1/5)
BsoBI 26 CTCGGG C^[CT]CG[GA]G
BstYI 645 GGATCC [GA]^GATC[CT]
DpnII 646 GATC ^GATC
DraI 493 TTTAAA TTT^AAA
DraII 81 GGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 397 CCATGG C^C[GA][CT]GG
EcoO109I 81 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT22I 488 ATGCAT ATGCA^T
HgaI 45 GACGC GACGC(5/10)
Hin1I 51 GGCGCC G[GA]^CG[CT]C
HincII 679 GTCAAC GT[CT]^[GA]AC
HindII 679 GTCAAC GT[CT]^[GA]AC
KasI 51 GGCGCC G^GCGCC
KpnI 393 GGTACC GGTAC^C
MboI 646 GATC ^GATC
MboII 183 TCTTC GAAGA(8/7)
MflI 645 GGATCC [GA]^GATC[CT]
MslI 508 CATGCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 51 GGCGCC GG^CGCC
NcoI 397 CCATGG C^CATGG
NdeII 646 GATC ^GATC
NsiI 488 ATGCAT ATGCA^T
PpuMI 81 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 629 GACATAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Sau3AI 646 GATC ^GATC
SexAI 462 ACCTGGT A^CC[AT]GGT
SfaNI 487 GATGC GCATC(5/9)
SfoI 51 GGCGCC GGC^GCC
SmlI 130 CTTAAG C^T[CT][GA]AG
SpeI 537 ACTAGT A^CTAGT
SspI 102 AATATT AAT^ATT
TaqI 285 TCGA T^CGA
TfiI 714 GATTC G^A[AT]TC
TthHB8I 285 TCGA T^CGA
XhoII 645 GGATCC [GA]^GATC[CT]
XmnI 147 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AfaI 394 GTAC GT^AC
561 GTAC
AlwI 646 GATCC GGATC(4/5)
645 GGATC
AspHI 63 GTGCTC G[AT]GC[AT]^C
566 GAGCAC
BalI 308 TGGCCA TGG^CCA
443 TGGCCA
BanI 51 GGCGCC G^G[CT][GA]CC
393 GGTACC
BbvI 619 GCAGC GCAGC(8/12)
661 GCAGC
BmyI 63 GTGCTC G[AGT]GC[ACT]^C
566 GAGCAC
BsaJI 305 CCTTGG C^C[ACGT][ACGT]GG
397 CCATGG
BsgI 605 GTGCAG GTGCAG(16/14)
617 GTGCAG
BsiHKAI 63 GTGCTC G[AT]GC[AT]^C
566 GAGCAC
BsmFI 81 GGGAC GGGAC(10/14)
636 GTCCC
Bsp1286I 63 GTGCTC G[AGT]GC[ACT]^C
566 GAGCAC
BsrI 215 ACTGG ACTGG(1/-1)
441 ACTGG
BssKI 156 CCTGG ^CC[ACGT]GG
463 CCTGG
BstAPI 502 GCAGTTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
699 GCAGAATATGC
BstNI 156 CCTGG CC^[AT]GG
463 CCTGG
BstXI 472 CCAGCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
650 CCAGAGTTGTGG
Cfr13I 82 GGACC G^G[ACGT]CC
469 GGGCC
EaeI 308 TGGCCA [CT]^GGCC[GA]
443 TGGCCA
EcoRII 156 CCTGG ^CC[AT]GG
463 CCTGG
EcoT14I 305 CCTTGG C^C[AT][AT]GG
397 CCATGG
Fnu4HI 619 GCAGC GC^[ACGT]GC
661 GCAGC
HphI 484 GGTGA GGTGA(8/7)
730 TCACC
ItaI 619 GCAGC GC^[ACGT]GC
661 GCAGC
MluNI 308 TGGCCA TGG^CCA
443 TGGCCA
MscI 308 TGGCCA TGG^CCA
443 TGGCCA
MseI 131 TTAA T^TAA
494 TTAA
MspA1I 620 CAGCTG C[AC]G^C[GT]G
662 CAGCTG
MvaI 156 CCTGG CC^[AT]GG
463 CCTGG
MwoI 502 GCAGTTCATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
699 GCAGAATATGC
PleI 24 GACTC GAGTC(4/5)
431 GAGTC
PvuII 620 CAGCTG CAG^CTG
662 CAGCTG
RsaI 394 GTAC GT^AC
561 GTAC
Sau96I 82 GGACC G^G[ACGT]CC
469 GGGCC
ScrFI 156 CCTGG CC^[ACGT]GG
463 CCTGG
StyI 305 CCTTGG C^C[AT][AT]GG
397 CCATGG
Tru9I 131 TTAA T^TAA
494 TTAA
TseI 619 GCAGC G^C[AT]GC
661 GCAGC
TspRI 248 CAGTG CA[GC]TG[ACGT][ACGT]^
734 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AcsI AluI ApoI BfaI BsiYI
BslI Cac8I CfoI DdeI HaeII
HaeIII HhaI HinP1I HinfI MaeI
MnlI NlaIII NlaIV Tsp509I