(Circular) (Six-base) MAPSORT of: pFN21AE6635.seq Check: 8896 from: 1 to: 5446 pFN21AE6635 5446 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3256 278 Size: 53 83 186 936 1720 2468 Fragments arranged by size: 2468 1720 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2789 1937 Size: 852 4594 Acc65I G'GTAC_C Cuts at: 2398 2767 2398 Size: 369 5077 AclI AA'CG_TT Cuts at: 1563 3573 3946 1563 Size: 2010 373 3063 Fragments arranged by size: 3063 2010 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2316 3509 4893 5161 1632 Size: 684 1193 1384 268 1917 Fragments arranged by size: 1917 1384 1193 684 268 AflII C'TTAA_G Cuts at: 820 1017 2135 820 Size: 197 1118 4131 Fragments arranged by size: 4131 1118 197 AflIII A'CryG_T Cuts at: 1203 4345 5322 1203 Size: 3142 977 1327 Fragments arranged by size: 3142 1327 977 AgeI A'CCGG_T Cuts at: 4277 4277 Size: 5446 AhdI GACnn_n'nnGTC Cuts at: 4175 4175 Size: 5446 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5446 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5446 AlwNI CAG_nnn'CTG Cuts at: 3189 4761 3189 Size: 1572 3874 ApaLI G'TGCA_C Cuts at: 3503 4659 3503 Size: 1156 4290 ApoI r'AATT_y Cuts at: 1493 2117 2167 2231 2353 2755 2981 3133 5242 Size: 624 50 64 122 402 226 152 2109 Cuts at: 5242 1493 Size: 1697 Fragments arranged by size: 2109 1697 624 402 226 152 122 64 50 AseI AT'TA_AT Cuts at: 160 1032 4000 160 Size: 872 2968 1606 Fragments arranged by size: 2968 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5446 AvaI C'yCGr_G Cuts at: 1870 1943 2031 2771 1870 Size: 73 88 740 4545 Fragments arranged by size: 4545 740 88 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2209 2242 2390 2423 2209 Size: 33 148 33 5232 Fragments arranged by size: 5232 148 33 33 BamHI G'GATC_C Cuts at: 2650 2776 3143 2650 Size: 126 367 4953 Fragments arranged by size: 4953 367 126 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2056 2398 2767 4222 618 Size: 325 217 627 269 342 369 1455 1842 Fragments arranged by size: 1842 1455 627 369 342 325 269 217 BanII G_rGCy'C Cuts at: 1391 2765 1391 Size: 1374 4072 BbeI G_GCGC'C Cuts at: 2060 2060 Size: 5446 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4475 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3637 3671 1903 Size: 34 50 34 1616 34 3678 Fragments arranged by size: 3678 1616 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2210 3338 4548 2210 Size: 1128 1210 3108 Fragments arranged by size: 3108 1210 1128 BclI T'GATC_A Cuts at: 1548 1548 Size: 5446 BfrBI ATG'CAT Cuts at: 2495 2495 Size: 5446 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4057 136 Size: 107 122 71 631 605 2385 1525 Fragments arranged by size: 2385 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5441 5441 Size: 5446 BlpI GC'TnA_GC Cuts at: 2863 2863 Size: 5446 Bme1580I G_kGCm'C Cuts at: 1790 3507 4663 1790 Size: 1717 1156 2573 Fragments arranged by size: 2573 1717 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5446 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3177 4135 442 Size: 416 53 83 399 24 1760 958 1753 Fragments arranged by size: 1760 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5446 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2242 4106 1239 Size: 1003 1864 2579 Fragments arranged by size: 2579 1864 1003 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3571 4436 4734 3571 Size: 865 298 4283 Fragments arranged by size: 4283 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4109 882 Size: 617 2610 2219 Fragments arranged by size: 2610 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3237 493 Size: 711 2033 2702 Fragments arranged by size: 2702 2033 711 BsaBI GATnn'nnATC Cuts at: 5440 5440 Size: 5446 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2057 3253 3635 4270 Size: 53 83 186 936 524 1196 382 635 Cuts at: 4270 275 Size: 1451 Fragments arranged by size: 1451 1196 936 635 524 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3879 4277 4551 4698 5109 3879 Size: 398 274 147 411 4216 Fragments arranged by size: 4216 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5221 Fragments arranged by size: 5221 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5446 BseYI C'CCAG_C Cuts at: 2627 4649 5145 2627 Size: 2022 496 2928 Fragments arranged by size: 2928 2022 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2631 2643 1163 Size: 573 895 12 3966 Fragments arranged by size: 3966 895 573 12 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2896 3657 4685 1540 Size: 275 186 895 761 1028 2301 Fragments arranged by size: 2301 1028 895 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2072 2575 3507 3592 4663 2072 Size: 503 932 85 1071 2855 Fragments arranged by size: 2855 1071 932 503 85 BsmI GAATG_Cn' Cuts at: 2957 3050 2957 Size: 93 5353 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2072 2575 2765 3507 3592 4663 1391 Size: 399 282 503 190 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 503 399 282 190 85 BspEI T'CCGG_A Cuts at: 5109 5109 Size: 5446 BspHI T'CATG_A Cuts at: 3335 5065 3335 Size: 1730 3716 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2801 844 Size: 1957 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3333 1394 Size: 429 1510 3507 Fragments arranged by size: 3507 1510 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3941 4115 65 Size: 1183 2693 174 1396 Fragments arranged by size: 2693 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4090 4277 1700 Size: 110 2280 187 2869 Fragments arranged by size: 2869 2280 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5446 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5446 BssSI C'ACGA_G Cuts at: 3506 4518 3506 Size: 1012 4434 BstAPI GCAn_nnn'nTGC Cuts at: 794 2513 2710 794 Size: 1719 197 3530 Fragments arranged by size: 3530 1719 197 BstBI TT'CG_AA Cuts at: 4250 4250 Size: 5446 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5446 BstXI CCAn_nnnn'nTGG Cuts at: 2484 2662 2484 Size: 178 5268 BstYI r'GATC_y Cuts at: 1972 2650 2776 3143 3528 3545 4997 5083 5095 Size: 678 126 367 385 17 1452 86 12 Cuts at: 5095 5441 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 678 385 367 346 126 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2402 513 Size: 552 1337 3557 Fragments arranged by size: 3557 1337 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4707 Fragments arranged by size: 4707 683 56 BtsI GCAGTG_nn' Cuts at: 2962 3756 3776 2962 Size: 794 20 4632 Fragments arranged by size: 4632 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5446 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5411 DraI TTT'AAA Cuts at: 2500 2750 2500 Size: 250 5196 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4453 809 Size: 507 3137 1802 Fragments arranged by size: 3137 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2313 2448 2893 Size: 54 1425 50 275 18 483 135 445 Cuts at: 2893 3782 5411 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 483 445 275 135 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2893 1537 Size: 275 1081 4090 Fragments arranged by size: 4090 1081 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3376 1353 Size: 2023 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4033 4407 4553 387 Size: 1064 138 2444 374 146 1280 Fragments arranged by size: 2444 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2242 2316 3509 4106 4893 5161 1239 Size: 393 610 74 1193 597 787 268 1524 Fragments arranged by size: 1524 1193 787 610 597 393 268 74 EcoO109I rG'GnC_Cy Cuts at: 2087 2087 Size: 5446 EcoRI G'AATT_C Cuts at: 2755 3133 2755 Size: 378 5068 FspI TGC'GCA Cuts at: 3952 3952 Size: 5446 HaeII r_GCGC'y Cuts at: 1678 2025 2039 2060 4593 1678 Size: 347 14 21 2533 2531 Fragments arranged by size: 2533 2531 347 21 14 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2377 2409 4087 4119 4161 Size: 32 135 32 805 32 1678 32 42 Cuts at: 4161 4193 1373 Size: 32 2626 Fragments arranged by size: 2626 1678 805 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2686 2790 3036 669 Size: 988 281 748 104 246 3079 Fragments arranged by size: 3079 988 748 281 246 104 HindIII A'AGCT_T Cuts at: 748 748 Size: 5446 HpaI GTT'AAC Cuts at: 3036 3036 Size: 5446 KasI G'GCGC_C Cuts at: 2056 2056 Size: 5446 KpnI G_GTAC'C Cuts at: 2402 2771 2402 Size: 369 5077 MfeI C'AATT_G Cuts at: 3045 3045 Size: 5446 MluI A'CGCG_T Cuts at: 5322 5322 Size: 5446 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2194 2527 4560 4744 1305 Size: 889 333 2033 184 2007 Fragments arranged by size: 2033 2007 889 333 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2315 2450 10 Size: 54 1425 343 483 135 3006 Fragments arranged by size: 3006 1425 483 343 135 54 MslI CAynn'nnrTG Cuts at: 518 1166 2517 3404 3763 3922 518 Size: 648 1351 887 359 159 2042 Fragments arranged by size: 2042 1351 887 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2627 2669 2862 3539 4687 4932 1606 Size: 1021 42 193 677 1148 245 2120 Fragments arranged by size: 2120 1148 1021 677 245 193 42 NarI GG'CG_CC Cuts at: 2057 2057 Size: 5446 NcoI C'CATG_G Cuts at: 513 1065 2402 513 Size: 552 1337 3557 Fragments arranged by size: 3557 1337 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5446 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5446 NotI GC'GGCC_GC Cuts at: 2893 2893 Size: 5446 NsiI A_TGCA'T Cuts at: 2497 2497 Size: 5446 NspI r_CATG'y Cuts at: 1132 1229 2804 4349 1132 Size: 97 1575 1545 2229 Fragments arranged by size: 2229 1575 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5446 PciI A'CATG_T Cuts at: 4345 4345 Size: 5446 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5446 PfoI T'CCnGG_A Cuts at: 2160 2160 Size: 5446 PmeI GTTT'AAAC Cuts at: 2750 2750 Size: 5446 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2328 2360 2574 2606 3515 3547 5028 5060 2328 Size: 32 214 32 909 32 1481 32 2714 Fragments arranged by size: 2714 1481 909 214 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2087 2087 Size: 5446 PshAI GACnn'nnGTC Cuts at: 1613 2638 1613 Size: 1025 4421 PsiI TTA'TAA Cuts at: 3016 3016 Size: 5446 PstI C_TGCA'G Cuts at: 830 2798 830 Size: 1968 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5446 PvuII CAG'CTG Cuts at: 2627 2669 2627 Size: 42 5404 SalI G'TCGA_C Cuts at: 1936 2788 1936 Size: 852 4594 SanDI GG'GwC_CC Cuts at: 2087 2087 Size: 5446 SbfI CC_TGCA'GG Cuts at: 2798 2798 Size: 5446 ScaI AGT'ACT Cuts at: 3694 5406 3694 Size: 1712 3734 SexAI A'CCwGG_T Cuts at: 2467 2467 Size: 5446 SfcI C'TryA_G Cuts at: 826 1045 2794 3929 4610 4801 826 Size: 219 1749 1135 681 191 1471 Fragments arranged by size: 1749 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2058 2058 Size: 5446 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5446 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5446 SmaI CCC'GGG Cuts at: 1872 2773 1872 Size: 901 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2135 3550 4451 4713 820 Size: 197 926 192 1415 901 262 1553 Fragments arranged by size: 1553 1415 926 901 262 197 192 SnaBI TAC'GTA Cuts at: 493 3237 493 Size: 2744 2702 SpeI A'CTAG_T Cuts at: 152 2542 152 Size: 2390 3056 SphI G_CATG'C Cuts at: 1132 2804 1132 Size: 1672 3774 SspI AAT'ATT Cuts at: 5 52 2109 3370 5 Size: 47 2057 1261 2081 Fragments arranged by size: 2081 2057 1261 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5446 StyI C'CwwG_G Cuts at: 513 1065 2310 2402 2885 513 Size: 552 1245 92 483 3074 Fragments arranged by size: 3074 1245 552 483 92 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3473 3490 3643 3828 4273 325 Size: 1255 1893 17 153 185 445 1498 Fragments arranged by size: 1893 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3692 5404 96 Size: 274 80 33 51 1443 1715 1712 138 Fragments arranged by size: 1715 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2593 2625 5406 5438 2593 Size: 32 2781 32 2601 Fragments arranged by size: 2781 2601 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5446 XbaI T'CTAG_A Cuts at: 2782 2782 Size: 5446 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5446 XmaI C'CCGG_G Cuts at: 1870 2771 1870 Size: 901 4545 XmnI GAAnn'nnTTC Cuts at: 2156 3575 2156 Size: 1419 4027 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3254 276 Size: 53 83 186 936 1720 2468 Fragments arranged by size: 2468 1720 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SfiI SrfI SwaI XcmI