(Circular) (Six-base) MAPSORT of: pFN21AE2626.seq Check: 8865 from: 1 to: 5611 pFN21AE2626 5611 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2699 2699 Size: 5611 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3421 278 Size: 53 83 186 936 1885 2468 Fragments arranged by size: 2468 1885 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2954 1937 Size: 1017 4594 Acc65I G'GTAC_C Cuts at: 2932 2932 Size: 5611 AclI AA'CG_TT Cuts at: 1563 3738 4111 1563 Size: 2175 373 3063 Fragments arranged by size: 3063 2175 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2031 2285 2457 2463 3674 5058 5326 1632 Size: 399 254 172 6 1211 1384 268 1917 Fragments arranged by size: 1917 1384 1211 399 268 254 172 6 AflII C'TTAA_G Cuts at: 820 1017 2351 820 Size: 197 1334 4080 Fragments arranged by size: 4080 1334 197 AflIII A'CryG_T Cuts at: 1203 4510 5487 1203 Size: 3307 977 1327 Fragments arranged by size: 3307 1327 977 AgeI A'CCGG_T Cuts at: 4442 4442 Size: 5611 AhdI GACnn_n'nnGTC Cuts at: 4340 4340 Size: 5611 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5611 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2190 1184 Size: 1006 4605 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2037 2069 2172 2204 2037 Size: 32 103 32 5444 Fragments arranged by size: 5444 103 32 32 AlwNI CAG_nnn'CTG Cuts at: 2145 3354 4926 2145 Size: 1209 1572 2830 Fragments arranged by size: 2830 1572 1209 ApaLI G'TGCA_C Cuts at: 2712 3668 4824 2712 Size: 956 1156 3499 Fragments arranged by size: 3499 1156 956 ApoI r'AATT_y Cuts at: 1493 2060 2315 2487 2584 2920 3146 3298 5407 Size: 567 255 172 97 336 226 152 2109 Cuts at: 5407 1493 Size: 1697 Fragments arranged by size: 2109 1697 567 336 255 226 172 152 97 AseI AT'TA_AT Cuts at: 160 1032 4165 160 Size: 872 3133 1606 Fragments arranged by size: 3133 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5611 AvaI C'yCGr_G Cuts at: 1870 1943 2936 1870 Size: 73 993 4545 Fragments arranged by size: 4545 993 73 BamHI G'GATC_C Cuts at: 2941 3308 2941 Size: 367 5244 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2932 4387 618 Size: 325 217 627 1145 1455 1842 Fragments arranged by size: 1842 1455 1145 627 325 217 BanII G_rGCy'C Cuts at: 1391 2930 1391 Size: 1539 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4640 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3802 3836 1903 Size: 34 1865 34 3678 Fragments arranged by size: 3678 1865 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3503 4713 3503 Size: 1210 4401 BclI T'GATC_A Cuts at: 1548 1548 Size: 5611 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4222 136 Size: 107 122 71 631 605 2550 1525 Fragments arranged by size: 2550 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2878 5606 2878 Size: 2728 2883 BlpI GC'TnA_GC Cuts at: 3028 3028 Size: 5611 Bme1580I G_kGCm'C Cuts at: 1790 2716 3672 4828 1790 Size: 926 956 1156 2573 Fragments arranged by size: 2573 1156 956 926 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5611 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3342 4300 442 Size: 416 53 83 399 24 1925 958 1753 Fragments arranged by size: 1925 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5611 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2481 2481 Size: 5611 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4271 1239 Size: 3032 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2478 3736 4601 4899 2478 Size: 1258 865 298 3190 Fragments arranged by size: 3190 1258 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4274 882 Size: 617 2775 2219 Fragments arranged by size: 2775 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3402 493 Size: 711 2198 2702 Fragments arranged by size: 2702 2198 711 BsaBI GATnn'nnATC Cuts at: 2075 5605 2075 Size: 3530 2081 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3418 3800 4435 275 Size: 53 83 186 936 1885 382 635 1451 Fragments arranged by size: 1885 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4044 4442 4716 4863 5274 4044 Size: 398 274 147 411 4381 Fragments arranged by size: 4381 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5386 Fragments arranged by size: 5386 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5611 BseYI C'CCAG_C Cuts at: 2885 4814 5310 2885 Size: 1929 496 3186 Fragments arranged by size: 3186 1929 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2158 2769 1163 Size: 573 422 611 4005 Fragments arranged by size: 4005 611 573 422 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3061 3822 4850 1540 Size: 275 186 1060 761 1028 2301 Fragments arranged by size: 2301 1060 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2716 3672 3757 4828 2716 Size: 956 85 1071 3499 Fragments arranged by size: 3499 1071 956 85 BsmI GAATG_Cn' Cuts at: 3122 3215 3122 Size: 93 5518 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2716 2930 3672 3757 4828 1391 Size: 399 926 214 742 85 1071 2174 Fragments arranged by size: 2174 1071 926 742 399 214 85 BspEI T'CCGG_A Cuts at: 5274 5274 Size: 5611 BspHI T'CATG_A Cuts at: 3500 5230 3500 Size: 1730 3881 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2699 2966 844 Size: 1855 267 3489 Fragments arranged by size: 3489 1855 267 BsrBI CCG'CTC Cuts at: 1394 1823 3498 1394 Size: 429 1675 3507 Fragments arranged by size: 3507 1675 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2610 4106 4280 65 Size: 1183 1362 1496 174 1396 Fragments arranged by size: 1496 1396 1362 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4255 4442 1700 Size: 110 2445 187 2869 Fragments arranged by size: 2869 2445 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5611 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5611 BssSI C'ACGA_G Cuts at: 3671 4683 3671 Size: 1012 4599 BstAPI GCAn_nnn'nTGC Cuts at: 794 2777 794 Size: 1983 3628 BstBI TT'CG_AA Cuts at: 4415 4415 Size: 5611 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5611 BstYI r'GATC_y Cuts at: 1972 2278 2402 2878 2941 3308 3693 3710 5162 Size: 306 124 476 63 367 385 17 1452 Cuts at: 5162 5248 5260 5606 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 476 385 367 346 306 124 86 63 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5059 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4872 Fragments arranged by size: 4872 683 56 BtsI GCAGTG_nn' Cuts at: 2914 3127 3921 3941 2914 Size: 213 794 20 4584 Fragments arranged by size: 4584 794 213 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5611 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5576 DraI TTT'AAA Cuts at: 2915 2915 Size: 5611 DraIII CAC_nnn'GTG Cuts at: 2792 2792 Size: 5611 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4618 809 Size: 507 3302 1802 Fragments arranged by size: 3302 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3058 3947 5576 Size: 54 1425 50 275 18 1228 889 1629 Cuts at: 5576 8 Size: 43 Fragments arranged by size: 1629 1425 1228 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3058 1537 Size: 275 1246 4090 Fragments arranged by size: 4090 1246 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2157 2261 2643 3541 1353 Size: 804 104 382 898 3423 Fragments arranged by size: 3423 898 804 382 104 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4198 4572 4718 387 Size: 1064 138 2609 374 146 1280 Fragments arranged by size: 2609 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2031 2285 2457 2463 3674 4271 5058 Size: 393 399 254 172 6 1211 597 787 Cuts at: 5058 5326 1239 Size: 268 1524 Fragments arranged by size: 1524 1211 787 597 399 393 268 254 172 6 EcoNI CCTnn'n_nnAGG Cuts at: 2706 2706 Size: 5611 EcoO109I rG'GnC_Cy Cuts at: 2738 2738 Size: 5611 EcoRI G'AATT_C Cuts at: 2584 2920 3298 2584 Size: 336 378 4897 Fragments arranged by size: 4897 378 336 FspI TGC'GCA Cuts at: 4117 4117 Size: 5611 HaeII r_GCGC'y Cuts at: 1678 4758 1678 Size: 3080 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2193 2225 4252 4284 4326 Size: 32 135 32 621 32 2027 32 42 Cuts at: 4326 4358 1373 Size: 32 2626 Fragments arranged by size: 2626 2027 621 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2626 2955 3201 669 Size: 988 281 688 329 246 3079 Fragments arranged by size: 3079 988 688 329 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5611 HpaI GTT'AAC Cuts at: 3201 3201 Size: 5611 KpnI G_GTAC'C Cuts at: 2936 2936 Size: 5611 MfeI C'AATT_G Cuts at: 3210 3210 Size: 5611 MluI A'CGCG_T Cuts at: 5487 5487 Size: 5611 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2347 4725 4909 1305 Size: 1042 2378 184 2007 Fragments arranged by size: 2378 2007 1042 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3789 Fragments arranged by size: 3789 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2172 3569 3928 4087 518 Size: 648 1006 1397 359 159 2042 Fragments arranged by size: 2042 1397 1006 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2783 2871 3027 3704 4852 5097 1606 Size: 1177 88 156 677 1148 245 2120 Fragments arranged by size: 2120 1177 1148 677 245 156 88 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5059 NdeI CA'TA_TG Cuts at: 387 387 Size: 5611 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5611 NotI GC'GGCC_GC Cuts at: 3058 3058 Size: 5611 NspI r_CATG'y Cuts at: 1132 1229 2969 4514 1132 Size: 97 1740 1545 2229 Fragments arranged by size: 2229 1740 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5611 PciI A'CATG_T Cuts at: 4510 4510 Size: 5611 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5611 PmeI GTTT'AAAC Cuts at: 2915 2915 Size: 5611 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3680 3712 5193 5225 3680 Size: 32 1481 32 4066 Fragments arranged by size: 4066 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2738 2738 Size: 5611 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5611 PsiI TTA'TAA Cuts at: 3181 3181 Size: 5611 PstI C_TGCA'G Cuts at: 830 2512 2963 830 Size: 1682 451 3478 Fragments arranged by size: 3478 1682 451 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5611 PvuII CAG'CTG Cuts at: 2783 2871 2783 Size: 88 5523 SalI G'TCGA_C Cuts at: 1936 2953 1936 Size: 1017 4594 SapI GCTCTTCn'nnn_ Cuts at: 2157 2157 Size: 5611 SbfI CC_TGCA'GG Cuts at: 2963 2963 Size: 5611 ScaI AGT'ACT Cuts at: 2259 3859 5571 2259 Size: 1600 1712 2299 Fragments arranged by size: 2299 1712 1600 SfcI C'TryA_G Cuts at: 826 1045 2340 2384 2508 2959 4094 4775 4966 Size: 219 1295 44 124 451 1135 681 191 Cuts at: 4966 826 Size: 1471 Fragments arranged by size: 1471 1295 1135 681 451 219 191 124 44 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5611 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5611 SmaI CCC'GGG Cuts at: 1872 2938 1872 Size: 1066 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2351 2493 3715 4616 4878 820 Size: 197 926 408 142 1222 901 262 1553 Fragments arranged by size: 1553 1222 926 901 408 262 197 142 SnaBI TAC'GTA Cuts at: 493 3402 493 Size: 2909 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5611 SphI G_CATG'C Cuts at: 1132 2969 1132 Size: 1837 3774 SspI AAT'ATT Cuts at: 5 52 2119 3535 5 Size: 47 2067 1416 2081 Fragments arranged by size: 2081 2067 1416 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5611 StyI C'CwwG_G Cuts at: 513 1065 3050 513 Size: 552 1985 3074 Fragments arranged by size: 3074 1985 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3638 3655 3808 3993 4438 325 Size: 1255 2058 17 153 185 445 1498 Fragments arranged by size: 2058 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2257 3857 5569 Size: 274 80 33 51 1443 280 1600 1712 Cuts at: 5569 96 Size: 138 Fragments arranged by size: 1712 1600 1443 280 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5571 5603 5571 Size: 32 5579 Tth111I GACn'n_nGTC Cuts at: 1316 2621 1316 Size: 1305 4306 XbaI T'CTAG_A Cuts at: 2947 2947 Size: 5611 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5611 XmaI C'CCGG_G Cuts at: 1870 2936 1870 Size: 1066 4545 XmnI GAAnn'nnTTC Cuts at: 3740 3740 Size: 5611 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3419 276 Size: 53 83 186 936 1885 2468 Fragments arranged by size: 2468 1885 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI