################################## # NO CUT ENZYMES ( 145 enzymes) # ################################## AatII AccI AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BclI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaBI BsaI BsiEI BsiWI BsmBI BsmFI BsoBI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI HaeII HincII HindII HindIII HpaI KasI Ksp632I KspI MamI MfeI MflI MluI MseI MslI MunI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tth111I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### Acc65I 86 GGTACC G^GTACC AccIII 455 TCCGGA T^CCGGA AciI 47 CCGC CCGC(-3/-1) AcyI 378 GACGCC G[GA]^CG[CT]C AlwI 825 GGATC GGATC(4/5) AlwNI 490 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 730 GGGCCC GGGCC^C Asp700I 309 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 86 GGTACC G^GTACC AspHI 434 GTGCTC G[AT]GC[AT]^C AvaII 910 GGTCC G^G[AT]CC BalI 224 TGGCCA TGG^CCA BanI 86 GGTACC G^G[CT][GA]CC BcgI 754 GCAGGCCATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 75 GGATAC GTATCC(6/5) BfaI 551 CTAG C^TAG BsaAI 373 TACGTG [CT]AC^GT[GA] BsaHI 378 GACGCC G[GA]^CG[CT]C BsaJI 201 CCAAGG C^C[ACGT][ACGT]GG BsaWI 455 TCCGGA [AT]^CCGG[AT] BseAI 455 TCCGGA T^CCGGA BseRI 141 GAGGAG GAGGAG(10/8) BsgI 181 CTGCAC GTGCAG(16/14) BsiHKAI 434 GTGCTC G[AT]GC[AT]^C BsmAI 475 GTCTC GTCTC(1/5) BsmI 231 GAATGC GAATGC(1/-1) BspEI 455 TCCGGA T^CCGGA BsrDI 533 GCAATG GCAATG(2/0) BstXI 344 CCAAATCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 122 GCGC GCG^C EaeI 224 TGGCCA [CT]^GGCC[GA] EcoT14I 201 CCAAGG C^C[AT][AT]GG HapII 456 CCGG C^CGG HhaI 122 GCGC GCG^C Hin1I 378 GACGCC G[GA]^CG[CT]C HinP1I 122 GCGC G^CGC HpaII 456 CCGG C^CGG HphI 241 GGTGA GGTGA(8/7) KpnI 86 GGTACC GGTAC^C MaeI 551 CTAG C^TAG MaeII 374 ACGT A^CGT MluNI 224 TGGCCA TGG^CCA MroI 455 TCCGGA T^CCGGA MscI 224 TGGCCA TGG^CCA MspI 456 CCGG C^CGG NdeI 608 CATATG CA^TATG PflMI 66 CCAAAATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 589 GACTC GAGTC(4/5) PstI 807 CTGCAG CTGCA^G PvuII 903 CAGCTG CAG^CTG SfaNI 751 GATGC GCATC(5/9) SfcI 807 CTGCAG C^T[GA][CT]AG SmlI 515 CTCAAG C^T[CT][GA]AG StuI 582 AGGCCT AGG^CCT StyI 201 CCAAGG C^C[AT][AT]GG Van91I 66 CCAAAATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 201 CCAAGGGAAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 309 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AccII 123 CGCG CG^CG 509 CGCG AfaI 87 GTAC GT^AC 636 GTAC AluI 904 AGCT AG^CT 988 AGCT BanII 730 GGGCCC G[GA]GC[CT]^C 921 GGGCTC BstBI 284 TTCGAA TT^CGAA 870 TTCGAA BstUI 123 CGCG CG^CG 509 CGCG DpnII 337 GATC ^GATC 826 GATC EcoRI 259 GAATTC G^AATTC 648 GAATTC HgaI 378 GACGC GACGC(5/10) 510 GCGTC MaeIII 242 GTGAC ^GT[ACGT]AC 376 GTGAC MboI 337 GATC ^GATC 826 GATC MspA1I 47 CCGCTG C[AC]G^C[GT]G 903 CAGCTG MvnI 123 CGCG CG^CG 509 CGCG NdeII 337 GATC ^GATC 826 GATC NspV 284 TTCGAA TT^CGAA 870 TTCGAA RsaI 87 GTAC GT^AC 636 GTAC Sau3AI 337 GATC ^GATC 826 GATC SfuI 284 TTCGAA TT^CGAA 870 TTCGAA Tsp45I 242 GTGAC ^GT[GC]AC 376 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AcsI ApoI BbvI BmyI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HinfI ItaI MboII MnlI MvaI MwoI NlaIII NlaIV Sau96I ScrFI TaqI TfiI TseI Tsp509I TspRI TthHB8I