(Circular) (Six-base) MAPSORT of: pFN21AE5448.seq Check: 9431 from: 1 to: 5707 pFN21AE5448 5707 bp With 182 enzymes: SgfI * January 30, 2014 17:20 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3517 278 Size: 53 83 186 936 1981 2468 Fragments arranged by size: 2468 1981 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3050 1937 Size: 1113 4594 Acc65I G'GTAC_C Cuts at: 2091 3028 2091 Size: 937 4770 AclI AA'CG_TT Cuts at: 1563 3834 4207 1563 Size: 2271 373 3063 Fragments arranged by size: 3063 2271 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3770 5154 5422 1632 Size: 2138 1384 268 1917 Fragments arranged by size: 2138 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5510 AflIII A'CryG_T Cuts at: 1203 4606 5583 1203 Size: 3403 977 1327 Fragments arranged by size: 3403 1327 977 AgeI A'CCGG_T Cuts at: 4538 4538 Size: 5707 AhdI GACnn_n'nnGTC Cuts at: 4436 4436 Size: 5707 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5707 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5707 AlwNI CAG_nnn'CTG Cuts at: 2500 3450 5022 2500 Size: 950 1572 3185 Fragments arranged by size: 3185 1572 950 ApaI G_GGCC'C Cuts at: 2739 2739 Size: 5707 ApaLI G'TGCA_C Cuts at: 3764 4920 3764 Size: 1156 4551 ApoI r'AATT_y Cuts at: 1493 2063 2074 2264 2300 2653 2872 3016 3242 Size: 570 11 190 36 353 219 144 226 Cuts at: 3242 3394 5503 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 570 353 226 219 190 152 144 36 11 AseI AT'TA_AT Cuts at: 160 1032 4261 160 Size: 872 3229 1606 Fragments arranged by size: 3229 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5707 AvaI C'yCGr_G Cuts at: 1870 1943 3032 1870 Size: 73 1089 4545 Fragments arranged by size: 4545 1089 73 BamHI G'GATC_C Cuts at: 3037 3404 3037 Size: 367 5340 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2091 3028 4483 618 Size: 325 217 627 304 937 1455 1842 Fragments arranged by size: 1842 1455 937 627 325 304 217 BanII G_rGCy'C Cuts at: 1391 2739 2930 3026 1391 Size: 1348 191 96 4072 Fragments arranged by size: 4072 1348 191 96 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4736 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2748 2782 3898 3932 1903 Size: 34 811 34 1116 34 3678 Fragments arranged by size: 3678 1116 811 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2074 3599 4809 2074 Size: 1525 1210 2972 Fragments arranged by size: 2972 1525 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5707 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4318 136 Size: 107 122 71 631 605 2646 1525 Fragments arranged by size: 2646 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5702 5702 Size: 5707 BlpI GC'TnA_GC Cuts at: 3124 3124 Size: 5707 Bme1580I G_kGCm'C Cuts at: 1790 2739 3768 4924 1790 Size: 949 1029 1156 2573 Fragments arranged by size: 2573 1156 1029 949 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5707 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2478 3438 4396 Size: 416 53 83 399 24 1061 960 958 Cuts at: 4396 442 Size: 1753 Fragments arranged by size: 1753 1061 960 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5707 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4367 1239 Size: 3128 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2505 3832 4697 4995 2505 Size: 1327 865 298 3217 Fragments arranged by size: 3217 1327 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4370 882 Size: 617 2871 2219 Fragments arranged by size: 2871 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2380 3498 493 Size: 711 1176 1118 2702 Fragments arranged by size: 2702 1176 1118 711 BsaBI GATnn'nnATC Cuts at: 5701 5701 Size: 5707 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2384 3514 3896 4531 Size: 53 83 186 936 851 1130 382 635 Cuts at: 4531 275 Size: 1451 Fragments arranged by size: 1451 1130 936 851 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2460 4140 4538 4812 4959 5370 2460 Size: 1680 398 274 147 411 2797 Fragments arranged by size: 2797 1680 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5482 Fragments arranged by size: 5482 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2161 1186 Size: 975 4732 BseYI C'CCAG_C Cuts at: 2910 4910 5406 2910 Size: 2000 496 3211 Fragments arranged by size: 3211 2000 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2171 1163 Size: 573 435 4699 Fragments arranged by size: 4699 573 435 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3157 3918 4946 1540 Size: 275 186 1156 761 1028 2301 Fragments arranged by size: 2301 1156 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2443 3768 3853 4924 2443 Size: 1325 85 1071 3226 Fragments arranged by size: 3226 1325 1071 85 BsmI GAATG_Cn' Cuts at: 2242 3218 3311 2242 Size: 976 93 4638 Fragments arranged by size: 4638 976 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2443 2739 2930 3026 3768 3853 4924 Size: 399 653 296 191 96 742 85 1071 Cuts at: 4924 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 653 399 296 191 96 85 BspEI T'CCGG_A Cuts at: 2460 5370 2460 Size: 2910 2797 BspHI T'CATG_A Cuts at: 3596 5326 3596 Size: 1730 3977 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3062 844 Size: 2218 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3594 1394 Size: 429 1771 3507 Fragments arranged by size: 3507 1771 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2545 4202 4376 65 Size: 1183 1297 1657 174 1396 Fragments arranged by size: 1657 1396 1297 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4351 4538 1700 Size: 110 2541 187 2869 Fragments arranged by size: 2869 2541 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5707 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5707 BssSI C'ACGA_G Cuts at: 3767 4779 3767 Size: 1012 4695 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5707 BstBI TT'CG_AA Cuts at: 2290 2876 4511 2290 Size: 586 1635 3486 Fragments arranged by size: 3486 1635 586 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5707 BstXI CCAn_nnnn'nTGG Cuts at: 2356 2356 Size: 5707 BstYI r'GATC_y Cuts at: 1972 3037 3404 3789 3806 5258 5344 5356 5702 Size: 1065 367 385 17 1452 86 12 346 Cuts at: 5702 1972 Size: 1977 Fragments arranged by size: 1977 1452 1065 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5155 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4968 Fragments arranged by size: 4968 683 56 BtsI GCAGTG_nn' Cuts at: 2160 2628 3223 4017 4037 2160 Size: 468 595 794 20 3830 Fragments arranged by size: 3830 794 595 468 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5707 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2953 2988 529 Size: 35 2389 35 3248 Fragments arranged by size: 3248 2389 35 35 DraI TTT'AAA Cuts at: 3011 3011 Size: 5707 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4714 809 Size: 507 3398 1802 Fragments arranged by size: 3398 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2229 3154 4043 Size: 54 1425 50 275 18 399 925 889 Cuts at: 4043 5672 8 Size: 1629 43 Fragments arranged by size: 1629 1425 925 889 399 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3154 1537 Size: 275 1342 4090 Fragments arranged by size: 4090 1342 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3637 1353 Size: 2284 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4294 4668 4814 387 Size: 1064 138 2705 374 146 1280 Fragments arranged by size: 2705 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3770 4367 5154 5422 1239 Size: 393 2138 597 787 268 1524 Fragments arranged by size: 2138 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2559 2707 2735 2559 Size: 148 28 5531 Fragments arranged by size: 5531 148 28 EcoRI G'AATT_C Cuts at: 2264 2653 3016 3394 2264 Size: 389 363 378 4577 Fragments arranged by size: 4577 389 378 363 FspI TGC'GCA Cuts at: 4213 4213 Size: 5707 HaeII r_GCGC'y Cuts at: 1678 4854 1678 Size: 3176 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2425 2457 4348 4380 4422 Size: 32 135 32 853 32 1891 32 42 Cuts at: 4422 4454 1373 Size: 32 2626 Fragments arranged by size: 2626 1891 853 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3051 3297 669 Size: 988 281 1113 246 3079 Fragments arranged by size: 3079 1113 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5707 HpaI GTT'AAC Cuts at: 3297 3297 Size: 5707 KpnI G_GTAC'C Cuts at: 2095 3032 2095 Size: 937 4770 MfeI C'AATT_G Cuts at: 3306 3306 Size: 5707 MluI A'CGCG_T Cuts at: 5583 5583 Size: 5707 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4821 5005 1305 Size: 3516 184 2007 Fragments arranged by size: 3516 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2231 10 Size: 54 1425 343 399 3486 Fragments arranged by size: 3486 1425 399 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3665 4024 4183 518 Size: 648 2499 359 159 2042 Fragments arranged by size: 2499 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2054 2910 3123 3800 4948 5193 1606 Size: 448 856 213 677 1148 245 2120 Fragments arranged by size: 2120 1148 856 677 448 245 213 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5155 NdeI CA'TA_TG Cuts at: 387 2614 387 Size: 2227 3480 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5707 NotI GC'GGCC_GC Cuts at: 3154 3154 Size: 5707 NspI r_CATG'y Cuts at: 1132 1229 3065 4610 1132 Size: 97 1836 1545 2229 Fragments arranged by size: 2229 1836 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5707 PciI A'CATG_T Cuts at: 4606 4606 Size: 5707 PflMI CCAn_nnn'nTGG Cuts at: 1108 2077 1108 Size: 969 4738 PmeI GTTT'AAAC Cuts at: 3011 3011 Size: 5707 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3776 3808 5289 5321 3776 Size: 32 1481 32 4162 Fragments arranged by size: 4162 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5707 PsiI TTA'TAA Cuts at: 3277 3277 Size: 5707 PspOMI G'GGCC_C Cuts at: 2735 2735 Size: 5707 PstI C_TGCA'G Cuts at: 830 2816 3059 830 Size: 1986 243 3478 Fragments arranged by size: 3478 1986 243 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5707 PvuII CAG'CTG Cuts at: 2910 2910 Size: 5707 SalI G'TCGA_C Cuts at: 1936 3049 1936 Size: 1113 4594 SbfI CC_TGCA'GG Cuts at: 3059 3059 Size: 5707 ScaI AGT'ACT Cuts at: 3955 5667 3955 Size: 1712 3995 SfcI C'TryA_G Cuts at: 826 1045 2812 3055 4190 4871 5062 826 Size: 219 1767 243 1135 681 191 1471 Fragments arranged by size: 1767 1471 1135 681 243 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5707 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5707 SmaI CCC'GGG Cuts at: 1872 3034 1872 Size: 1162 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2520 3811 4712 4974 820 Size: 197 926 577 1291 901 262 1553 Fragments arranged by size: 1553 1291 926 901 577 262 197 SnaBI TAC'GTA Cuts at: 493 3498 493 Size: 3005 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5707 SphI G_CATG'C Cuts at: 1132 3065 1132 Size: 1933 3774 SspI AAT'ATT Cuts at: 5 52 3631 5 Size: 47 3579 2081 Fragments arranged by size: 3579 2081 47 StuI AGG'CCT Cuts at: 1517 2589 1517 Size: 1072 4635 StyI C'CwwG_G Cuts at: 513 1065 2206 3146 513 Size: 552 1141 940 3074 Fragments arranged by size: 3074 1141 940 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3734 3751 3904 4089 4534 325 Size: 1255 2154 17 153 185 445 1498 Fragments arranged by size: 2154 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2640 3953 5665 Size: 274 80 33 51 1443 663 1313 1712 Cuts at: 5665 96 Size: 138 Fragments arranged by size: 1712 1443 1313 663 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2072 2104 5667 5699 2072 Size: 32 3563 32 2080 Fragments arranged by size: 3563 2080 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5707 XbaI T'CTAG_A Cuts at: 3043 3043 Size: 5707 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2213 2213 Size: 5707 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5707 XmaI C'CCGG_G Cuts at: 1870 3032 1870 Size: 1162 4545 XmnI GAAnn'nnTTC Cuts at: 2318 3836 2318 Size: 1518 4189 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3515 276 Size: 53 83 186 936 1981 2468 Fragments arranged by size: 2468 1981 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI