Restriction map for
pFN21AE5448
(Linear) (Six-base) MAPSORT of: KIEE5448.seq Check: 6580 from: 1 to: 1002
KIEE5448.orf
With 182 enzymes: SgfI *
January 29, 2014 12:01 ..
Acc65I G'GTAC_C
Cuts at: 0 86 1002
Size: 86 916
AlwNI CAG_nnn'CTG
Cuts at: 0 495 1002
Size: 495 507
ApaI G_GGCC'C
Cuts at: 0 734 1002
Size: 734 268
ApoI r'AATT_y
Cuts at: 0 58 69 259 295 648 867 1002
Size: 58 11 190 36 353 219 135
Fragments arranged by size:
353 219 190 135 58 36 11
BanI G'GyrC_C
Cuts at: 0 86 1002
Size: 86 916
BanII G_rGCy'C
Cuts at: 0 734 925 1002
Size: 734 191 77
Fragments arranged by size:
734 191 77
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 743 777 1002
Size: 743 34 225
Fragments arranged by size:
743 225 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 69 1002
Size: 69 933
Bme1580I G_kGCm'C
Cuts at: 0 734 1002
Size: 734 268
BmrI ACTGGGnnnn_n'
Cuts at: 0 473 1002
Size: 473 529
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 500 1002
Size: 500 502
BsaAI yAC'GTr
Cuts at: 0 375 1002
Size: 375 627
BsaHI Gr'CG_yC
Cuts at: 0 379 1002
Size: 379 623
BsaWI w'CCGG_w
Cuts at: 0 455 1002
Size: 455 547
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 156 1002
Size: 156 846
BseYI C'CCAG_C
Cuts at: 0 905 1002
Size: 905 97
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 1002
Size: 166 836
BsiHKAI G_wGCw'C
Cuts at: 0 438 1002
Size: 438 564
BsmI GAATG_Cn'
Cuts at: 0 237 1002
Size: 237 765
Bsp1286I G_dGCh'C
Cuts at: 0 438 734 925 1002
Size: 438 296 191 77
Fragments arranged by size:
438 296 191 77
BspEI T'CCGG_A
Cuts at: 0 455 1002
Size: 455 547
BsrDI GCAATG_nn'
Cuts at: 0 540 1002
Size: 540 462
BstBI TT'CG_AA
Cuts at: 0 285 871 1002
Size: 285 586 131
Fragments arranged by size:
586 285 131
BstXI CCAn_nnnn'nTGG
Cuts at: 0 351 1002
Size: 351 651
BtsI GCAGTG_nn'
Cuts at: 0 155 623 1002
Size: 155 468 379
Fragments arranged by size:
468 379 155
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 948 983 1002
Size: 948 35 19
Fragments arranged by size:
948 35 19
EaeI y'GGCC_r
Cuts at: 0 224 1002
Size: 224 778
EcoO109I rG'GnC_Cy
Cuts at: 0 554 702 730 1002
Size: 554 148 28 272
Fragments arranged by size:
554 272 148 28
EcoRI G'AATT_C
Cuts at: 0 259 648 1002
Size: 259 389 354
Fragments arranged by size:
389 354 259
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 420 452 1002
Size: 420 32 550
Fragments arranged by size:
550 420 32
KpnI G_GTAC'C
Cuts at: 0 90 1002
Size: 90 912
MscI TGG'CCA
Cuts at: 0 226 1002
Size: 226 776
MspA1I CmG'CkG
Cuts at: 0 49 905 1002
Size: 49 856 97
Fragments arranged by size:
856 97 49
NdeI CA'TA_TG
Cuts at: 0 609 1002
Size: 609 393
PflMI CCAn_nnn'nTGG
Cuts at: 0 72 1002
Size: 72 930
PspOMI G'GGCC_C
Cuts at: 0 730 1002
Size: 730 272
PstI C_TGCA'G
Cuts at: 0 811 1002
Size: 811 191
PvuII CAG'CTG
Cuts at: 0 905 1002
Size: 905 97
SfcI C'TryA_G
Cuts at: 0 807 1002
Size: 807 195
SmlI C'TyrA_G
Cuts at: 0 515 1002
Size: 515 487
StuI AGG'CCT
Cuts at: 0 584 1002
Size: 584 418
StyI C'CwwG_G
Cuts at: 0 201 1002
Size: 201 801
TatI w'GTAC_w
Cuts at: 0 635 1002
Size: 635 367
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 67 99 1002
Size: 67 32 903
Fragments arranged by size:
903 67 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 208 1002
Size: 208 794
XmnI GAAnn'nnTTC
Cuts at: 0 313 1002
Size: 313 689
Enzymes that do cut:
Acc65I AlwNI ApaI ApoI BanI BanII BcgI BciVI
Bme1580I BmrI BpuEI BsaAI BsaHI BsaWI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspEI BsrDI BstBI BstXI
BtsI CspCI EaeI EcoO109I EcoRI Hin4I KpnI MscI
MspA1I NdeI PflMI PspOMI PstI PvuII SfcI SmlI
StuI StyI TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BbvCI
BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI
BpmI Bpu10I BsaI BsaBI BsaXI BsiEI BsiWI BsmBI
BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstEII BstYI BstZ17I Bsu36I BtgI BtgZI ClaI DraI
DraIII DrdI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI MfeI MluI MmeI MslI NaeI NarI
NcoI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TaqII Tth111I XbaI XhoI XmaI ZraI