################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII ClaI CpoI DpnII DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoO65I EcoT22I FbaI FseI FspI HgaI Hin1I HindIII HinfI HpaI KasI KpnI KspI MboI MfeI MflI MluI MluNI MroI MscI MunI NarI NcoI NdeI NdeII NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI RsrII SacI SacII SalI Sau3AI SbfI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TaqI TfiI Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AseI 858 ATTAAT AT^TAAT AsnI 858 ATTAAT AT^TAAT AspHI 364 GTGCTC G[AT]GC[AT]^C AvaI 466 CTCGGG C^[CT]CG[GA]G BanII 43 GAGCCC G[GA]GC[CT]^C BciVI 835 GTATCC GTATCC(6/5) BfaI 595 CTAG C^TAG BpmI 668 CTGGAG CTGGAG(16/14) BsaBI 855 GATATTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 671 GAGACC GGTCTC(1/5) BseRI 295 CTCCTC GAGGAG(10/8) BsgI 780 GTGCAG GTGCAG(16/14) BsiEI 5 CGGCCG CG[GA][CT]^CG BsiHKAI 364 GTGCTC G[AT]GC[AT]^C BsmFI 103 GGGAC GGGAC(10/14) BsoBI 466 CTCGGG C^[CT]CG[GA]G BspHI 632 TCATGA T^CATGA BsrFI 7 GCCGGC [GA]^CCGG[CT] BstF5I 441 CATCC GGATG(2/0) BstNI 15 CCAGG CC^[AT]GG BstXI 312 CCACATAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 7 GCCGGC [GA]^CCGG[CT] DraII 103 GGGACCT [GA]G^G[ACGT]CC[CT] DsaI 770 CCACGG C^C[GA][CT]GG EagI 5 CGGCCG C^GGCCG EclXI 5 CGGCCG C^GGCCG Eco52I 5 CGGCCG C^GGCCG EcoNI 675 CCTGCAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 103 GGGACCT [GA]G^G[ACGT]CC[CT] EcoRI 691 GAATTC G^AATTC EcoRII 15 CCAGG ^CC[AT]GG EcoRV 538 GATATC GAT^ATC EcoT14I 177 CCTTGG C^C[AT][AT]GG FokI 441 CATCC GGATG(9/13) HincII 130 GTCAAC GT[CT]^[GA]AC HindII 130 GTCAAC GT[CT]^[GA]AC MaeI 595 CTAG C^TAG MaeII 302 ACGT A^CGT MaeIII 165 GTCAC ^GT[ACGT]AC MamI 855 GATATTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspA1I 482 CAGCTG C[AC]G^C[GT]G MvaI 15 CCAGG CC^[AT]GG NaeI 7 GCCGGC GCC^GGC NgoMIV 7 GCCGGC G^CCGGC PpuMI 103 GGGACCT [GA]G^G[AT]CC[CT] PshBI 858 ATTAAT AT^TAAT PvuII 482 CAGCTG CAG^CTG RcaI 632 TCATGA T^CATGA ScaI 609 AGTACT AGT^ACT SfcI 724 CTACAG C^T[GA][CT]AG SmlI 927 CTTGAG C^T[CT][GA]AG SspI 907 AATATT AAT^ATT StyI 177 CCTTGG C^C[AT][AT]GG Tsp45I 165 GTCAC ^GT[GC]AC XcmI 569 CCAAACAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AfaI 610 GTAC GT^AC 765 GTAC AvaII 104 GGACC G^G[AT]CC 582 GGACC BcnI 46 CCCGG CC^[GC]GG 77 CCCGG BmyI 43 GAGCCC G[AGT]GC[ACT]^C 364 GTGCTC Bsp1286I 43 GAGCCC G[AGT]GC[ACT]^C 364 GTGCTC BspMI 106 ACCTGC ACCTGC(4/8) 674 ACCTGC BsrI 667 ACTGG ACTGG(1/-1) 957 CCAGT EaeI 5 CGGCCG [CT]^GGCC[GA] 12 CGGCCA EarI 94 CTCTTC CTCTTC(1/4) 1005 GAAGAG FauI 38 GCGGG CCCGC(4/6) 69 GCGGG Ksp632I 94 CTCTTC CTCTTC(1/4) 1005 GAAGAG MslI 201 CACCATAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 313 CACATAAGTG NciI 46 CCCGG CC^[GC]GG 77 CCCGG NlaIII 633 CATG CATG^ 915 CATG RsaI 610 GTAC GT^AC 765 GTAC SapI 1005 GAAGAGC GCTCTTC(1/4) 93 GCTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AccII AciI AcsI AluI ApoI BbvI BsaJI BsiYI BslI BsmAI BssKI BstUI Cac8I CfoI Cfr13I DdeI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI MboII MnlI MseI MspI MvnI MwoI NlaIV Sau96I ScrFI Tru9I TseI Tsp509I TspRI