Restriction map for
pFN21AE6373
(Linear) (Six-base) MAPSORT of: KIEE6373.seq Check: 9436 from: 1 to: 1011
KIEE6373.orf
With 182 enzymes: SgfI *
March 16, 2015 11:30 ..
AleI CACnn'nnGTG
Cuts at: 0 317 1011
Size: 317 694
ApoI r'AATT_y
Cuts at: 0 266 373 691 1011
Size: 266 107 318 320
Fragments arranged by size:
320 318 266 107
AseI AT'TA_AT
Cuts at: 0 859 1011
Size: 859 152
AvaI C'yCGr_G
Cuts at: 0 466 1011
Size: 466 545
BanII G_rGCy'C
Cuts at: 0 47 1011
Size: 47 964
BciVI GTATCCnnnnn_n'
Cuts at: 0 846 1011
Size: 846 165
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 689 1011
Size: 689 322
Bpu10I CC'TnA_GC
Cuts at: 0 367 1011
Size: 367 644
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 948 1011
Size: 948 63
BsaI GGTCTCn'nnnn_
Cuts at: 0 665 1011
Size: 665 346
BsaBI GATnn'nnATC
Cuts at: 0 859 1011
Size: 859 152
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 286 1011
Size: 286 725
BseYI C'CCAG_C
Cuts at: 0 484 1011
Size: 484 527
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 801 1011
Size: 801 210
BsiEI CG_ry'CG
Cuts at: 0 8 1011
Size: 8 1003
BsiHKAI G_wGCw'C
Cuts at: 0 368 1011
Size: 368 643
Bsp1286I G_dGCh'C
Cuts at: 0 47 368 1011
Size: 47 321 643
Fragments arranged by size:
643 321 47
BspHI T'CATG_A
Cuts at: 0 632 1011
Size: 632 379
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 115 683 1011
Size: 115 568 328
Fragments arranged by size:
568 328 115
BsrFI r'CCGG_y
Cuts at: 0 7 1011
Size: 7 1004
BstXI CCAn_nnnn'nTGG
Cuts at: 0 319 1011
Size: 319 692
BtgI C'CryG_G
Cuts at: 0 770 1011
Size: 770 241
BtsI GCAGTG_nn'
Cuts at: 0 818 1011
Size: 818 193
EaeI y'GGCC_r
Cuts at: 0 5 12 1011
Size: 5 7 999
Fragments arranged by size:
999 7 5
EagI C'GGCC_G
Cuts at: 0 5 1011
Size: 5 1006
EarI CTCTTCn'nnn_
Cuts at: 0 100 1000 1011
Size: 100 900 11
Fragments arranged by size:
900 100 11
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 689 1011
Size: 689 322
EcoNI CCTnn'n_nnAGG
Cuts at: 0 679 1011
Size: 679 332
EcoO109I rG'GnC_Cy
Cuts at: 0 104 1011
Size: 104 907
EcoRI G'AATT_C
Cuts at: 0 691 1011
Size: 691 320
EcoRV GAT'ATC
Cuts at: 0 540 1011
Size: 540 471
HaeII r_GCGC'y
Cuts at: 0 35 94 142 1011
Size: 35 59 48 869
Fragments arranged by size:
869 59 48 35
HincII GTy'rAC
Cuts at: 0 132 1011
Size: 132 879
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 285 798 1011
Size: 285 513 213
Fragments arranged by size:
513 285 213
MslI CAynn'nnrTG
Cuts at: 0 205 317 1011
Size: 205 112 694
Fragments arranged by size:
694 205 112
MspA1I CmG'CkG
Cuts at: 0 484 1011
Size: 484 527
NaeI GCC'GGC
Cuts at: 0 9 1011
Size: 9 1002
NgoMIV G'CCGG_C
Cuts at: 0 7 1011
Size: 7 1004
PpuMI rG'GwC_Cy
Cuts at: 0 104 1011
Size: 104 907
PvuII CAG'CTG
Cuts at: 0 484 1011
Size: 484 527
SapI GCTCTTCn'nnn_
Cuts at: 0 100 1000 1011
Size: 100 900 11
Fragments arranged by size:
900 100 11
ScaI AGT'ACT
Cuts at: 0 611 1011
Size: 611 400
SfcI C'TryA_G
Cuts at: 0 724 1011
Size: 724 287
SmlI C'TyrA_G
Cuts at: 0 927 1011
Size: 927 84
SspI AAT'ATT
Cuts at: 0 909 1011
Size: 909 102
StyI C'CwwG_G
Cuts at: 0 177 1011
Size: 177 834
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 967 1011
Size: 967 44
TatI w'GTAC_w
Cuts at: 0 609 764 1011
Size: 609 155 247
Fragments arranged by size:
609 247 155
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 158 190 1011
Size: 158 32 821
Fragments arranged by size:
821 158 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 576 1011
Size: 576 435
Enzymes that do cut:
AleI ApoI AseI AvaI BanII BciVI BpmI Bpu10I
BpuEI BsaI BsaBI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspHI BspMI BsrFI BstXI BtgI BtsI EaeI
EagI EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII
HincII MmeI MslI MspA1I NaeI NgoMIV PpuMI PvuII
SapI ScaI SfcI SmlI SspI StyI TaqII TatI
TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AlfI AloI AlwNI ApaI ApaLI
AscI AsiSI AvrII BaeI BamHI BanI BbeI BbsI
BbvCI BcgI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BsaAI BsaHI BsaWI BsaXI
BsiWI BsmI BsmBI BspEI BsrBI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EciI EcoICRI FalI
FseI FspI FspAI Hin4I HindIII HpaI KasI KpnI
MfeI MluI MscI NarI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI SwaI Tth111I XbaI XhoI XmaI
XmnI ZraI