Restriction map for
pFN21AE6554
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# NO CUT ENZYMES ( 167 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DdeI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FokI FseI FspI
HaeII HindIII HpaI HphI KasI
KpnI KspI MaeI MaeIII MamI
MfeI MflI MroI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspBI SspI StuI
StyI SwaI TaqI TfiI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AcyI 67 GACGCC G[GA]^CG[CT]C
AflIII 179 ACGCGT A^C[GA][CT]GT
AspHI 25 GAGCTC G[AT]GC[AT]^C
AvaI 6 CTCGGG C^[CT]CG[GA]G
BanI 444 GGCACC G^G[CT][GA]CC
BglI 70 GCCTCGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 90 CTGGAG CTGGAG(16/14)
BsaHI 67 GACGCC G[GA]^CG[CT]C
BseRI 16 GAGGAG GAGGAG(10/8)
BsiHKAI 25 GAGCTC G[AT]GC[AT]^C
BsmFI 183 GTCCC GGGAC(10/14)
BsoBI 6 CTCGGG C^[CT]CG[GA]G
BsrFI 75 GCCGGC [GA]^CCGG[CT]
BstXI 365 CCACGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 75 GCCGGC [GA]^CCGG[CT]
DpnII 273 GATC ^GATC
DsaI 365 CCACGG C^C[GA][CT]GG
EarI 132 GAAGAG CTCTTC(1/4)
FauI 80 CCCGC CCCGC(4/6)
Hin1I 67 GACGCC G[GA]^CG[CT]C
HincII 394 GTCAAC GT[CT]^[GA]AC
HindII 394 GTCAAC GT[CT]^[GA]AC
HinfI 4 GACTC G^A[ACGT]TC
Ksp632I 132 GAAGAG CTCTTC(1/4)
MaeII 110 ACGT A^CGT
MboI 273 GATC ^GATC
MluI 179 ACGCGT A^CGCGT
MseI 317 TTAA T^TAA
NaeI 75 GCCGGC GCC^GGC
NdeII 273 GATC ^GATC
NgoMIV 75 GCCGGC G^CCGGC
NspI 149 GCATGC [GA]CATG^[CT]
PleI 4 GACTC GAGTC(4/5)
PstI 268 CTGCAG CTGCA^G
PvuII 187 CAGCTG CAG^CTG
SacI 25 GAGCTC GAGCT^C
SapI 132 GAAGAGC GCTCTTC(1/4)
Sau3AI 273 GATC ^GATC
SfcI 268 CTGCAG C^T[GA][CT]AG
SphI 149 GCATGC GCATG^C
Tru9I 317 TTAA T^TAA
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccII 162 CGCG CG^CG
180 CGCG
AciI 81 CCGC CCGC(-3/-1)
35 GCGG
AfaI 21 GTAC GT^AC
309 GTAC
ApaI 100 GGGCCC GGGCC^C
210 GGGCCC
AvaII 243 GGTCC G^G[AT]CC
420 GGTCC
BalI 173 TGGCCA TGG^CCA
362 TGGCCA
BstUI 162 CGCG CG^CG
180 CGCG
CfoI 137 GCGC GCG^C
163 GCGC
DraII 209 AGGGCCC [GA]G^G[ACGT]CC[CT]
210 GGGCCCC
EcoO109I 209 AGGGCCC [GA]G^G[ACGT]CC[CT]
210 GGGCCCC
HgaI 67 GACGC GACGC(5/10)
181 GCGTC
HhaI 137 GCGC GCG^C
163 GCGC
HinP1I 137 GCGC G^CGC
163 GCGC
MluNI 173 TGGCCA TGG^CCA
362 TGGCCA
MscI 173 TGGCCA TGG^CCA
362 TGGCCA
MslI 105 CATCAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
381 CATCATCGTG
MspA1I 81 CCGCTG C[AC]G^C[GT]G
187 CAGCTG
MvnI 162 CGCG CG^CG
180 CGCG
NlaIII 150 CATG CATG^
441 CATG
RsaI 21 GTAC GT^AC
309 GTAC
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AluI BanII BbvI BcnI BmyI
BsaJI BsiYI BslI Bsp1286I BssKI
BstNI Cac8I Cfr13I EaeI EcoRII
Fnu4HI HaeIII HapII HpaII ItaI
MboII MnlI MspI MvaI MwoI
NciI NlaIV Sau96I ScrFI TseI