(Circular) (Six-base) MAPSORT of: pFN21AE6554.seq Check: 1783 from: 1 to: 5161 pFN21AE6554 5161 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2971 278 Size: 53 83 186 936 1435 2468 Fragments arranged by size: 2468 1435 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2504 1937 Size: 567 4594 Acc65I G'GTAC_C Cuts at: 2482 2482 Size: 5161 AclI AA'CG_TT Cuts at: 1563 3288 3661 1563 Size: 1725 373 3063 Fragments arranged by size: 3063 1725 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2216 3224 4608 4876 1632 Size: 584 1008 1384 268 1917 Fragments arranged by size: 1917 1384 1008 584 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4964 AflIII A'CryG_T Cuts at: 1203 2184 4060 5037 1203 Size: 981 1876 977 1327 Fragments arranged by size: 1876 1327 981 977 AgeI A'CCGG_T Cuts at: 3992 3992 Size: 5161 AhdI GACnn_n'nnGTC Cuts at: 3890 3890 Size: 5161 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5161 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5161 AlwNI CAG_nnn'CTG Cuts at: 2904 4476 2904 Size: 1572 3589 ApaI G_GGCC'C Cuts at: 2109 2219 2109 Size: 110 5051 ApaLI G'TGCA_C Cuts at: 3218 4374 3218 Size: 1156 4005 ApoI r'AATT_y Cuts at: 1493 2470 2696 2848 4957 1493 Size: 977 226 152 2109 1697 Fragments arranged by size: 2109 1697 977 226 152 AseI AT'TA_AT Cuts at: 160 1032 3715 160 Size: 872 2683 1606 Fragments arranged by size: 2683 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5161 AvaI C'yCGr_G Cuts at: 1870 1943 2011 2486 1870 Size: 73 68 475 4545 Fragments arranged by size: 4545 475 73 68 BamHI G'GATC_C Cuts at: 2491 2858 2491 Size: 367 4794 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2449 2482 3937 618 Size: 325 217 627 662 33 1455 1842 Fragments arranged by size: 1842 1455 662 627 325 217 33 BanII G_rGCy'C Cuts at: 1391 2034 2109 2219 2480 1391 Size: 643 75 110 261 4072 Fragments arranged by size: 4072 643 261 110 75 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4190 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3352 3386 1903 Size: 34 1415 34 3678 Fragments arranged by size: 3678 1415 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3053 4263 3053 Size: 1210 3951 BclI T'GATC_A Cuts at: 1548 1548 Size: 5161 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2081 3772 136 Size: 107 122 71 631 605 409 1691 1525 Fragments arranged by size: 1691 1525 631 605 409 122 107 71 BglII A'GATC_T Cuts at: 5156 5156 Size: 5161 BlpI GC'TnA_GC Cuts at: 2578 2578 Size: 5161 Bme1580I G_kGCm'C Cuts at: 1790 2057 2109 2219 2241 3222 4378 1790 Size: 267 52 110 22 981 1156 2573 Fragments arranged by size: 2573 1156 981 267 110 52 22 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5161 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2892 3850 442 Size: 416 53 83 399 24 1475 958 1753 Fragments arranged by size: 1753 1475 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5161 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2116 3821 1239 Size: 877 1705 2579 Fragments arranged by size: 2579 1705 877 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3286 4151 4449 3286 Size: 865 298 3998 Fragments arranged by size: 3998 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3824 882 Size: 617 2325 2219 Fragments arranged by size: 2325 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2952 493 Size: 711 1748 2702 Fragments arranged by size: 2702 1748 711 BsaBI GATnn'nnATC Cuts at: 5155 5155 Size: 5161 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2073 2968 3350 3985 Size: 53 83 186 936 540 895 382 635 Cuts at: 3985 275 Size: 1451 Fragments arranged by size: 1451 936 895 635 540 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3594 3992 4266 4413 4824 3594 Size: 398 274 147 411 3931 Fragments arranged by size: 3931 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4936 Fragments arranged by size: 4936 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2036 1186 Size: 850 4311 BseYI C'CCAG_C Cuts at: 2088 2190 2219 2454 4364 4860 2088 Size: 102 29 235 1910 496 2389 Fragments arranged by size: 2389 1910 496 235 102 29 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4588 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2611 3372 4400 1540 Size: 275 186 610 761 1028 2301 Fragments arranged by size: 2301 1028 761 610 275 186 BsiHKAI G_wGCw'C Cuts at: 2034 3222 3307 4378 2034 Size: 1188 85 1071 2817 Fragments arranged by size: 2817 1188 1071 85 BsmI GAATG_Cn' Cuts at: 2672 2765 2672 Size: 93 5068 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2034 2057 2109 2219 2241 2480 3222 Size: 399 244 23 52 110 22 239 742 Cuts at: 3222 3307 4378 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 244 239 110 85 52 23 22 BspEI T'CCGG_A Cuts at: 4824 4824 Size: 5161 BspHI T'CATG_A Cuts at: 3050 4780 3050 Size: 1730 3431 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2449 2516 844 Size: 1605 67 3489 Fragments arranged by size: 3489 1605 67 BsrBI CCG'CTC Cuts at: 1394 1823 3048 1394 Size: 429 1225 3507 Fragments arranged by size: 3507 1225 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3656 3830 65 Size: 1183 2408 174 1396 Fragments arranged by size: 2408 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2080 3805 3992 1700 Size: 110 270 1725 187 2869 Fragments arranged by size: 2869 1725 270 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5161 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5161 BssSI C'ACGA_G Cuts at: 3221 4233 3221 Size: 1012 4149 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5161 BstBI TT'CG_AA Cuts at: 3965 3965 Size: 5161 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5161 BstXI CCAn_nnnn'nTGG Cuts at: 2377 2377 Size: 5161 BstYI r'GATC_y Cuts at: 1972 2491 2858 3243 3260 4712 4798 4810 5156 Size: 519 367 385 17 1452 86 12 346 Cuts at: 5156 1972 Size: 1977 Fragments arranged by size: 1977 1452 519 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2370 513 Size: 552 939 366 3304 Fragments arranged by size: 3304 939 552 366 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2149 487 Size: 683 56 923 3499 Fragments arranged by size: 3499 923 683 56 BtsI GCAGTG_nn' Cuts at: 2677 3471 3491 2677 Size: 794 20 4347 Fragments arranged by size: 4347 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5161 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5126 DraI TTT'AAA Cuts at: 2465 2465 Size: 5161 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4168 809 Size: 507 2852 1802 Fragments arranged by size: 2852 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2178 2331 2367 Size: 54 1425 50 275 18 348 153 36 Cuts at: 2367 2608 3497 5126 8 Size: 241 889 1629 43 Fragments arranged by size: 1629 1425 889 348 275 241 153 54 50 43 36 18 EagI C'GGCC_G Cuts at: 1537 1812 2608 1537 Size: 275 796 4090 Fragments arranged by size: 4090 796 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2132 3091 1353 Size: 779 959 3423 Fragments arranged by size: 3423 959 779 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3748 4122 4268 387 Size: 1064 138 2159 374 146 1280 Fragments arranged by size: 2159 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2032 2032 Size: 5161 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2116 2216 3224 3821 4608 4876 1239 Size: 393 484 100 1008 597 787 268 1524 Fragments arranged by size: 1524 1008 787 597 484 393 268 100 EcoO109I rG'GnC_Cy Cuts at: 2215 2216 2215 Size: 1 5160 EcoRI G'AATT_C Cuts at: 2470 2848 2470 Size: 378 4783 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2368 2368 Size: 5161 FspI TGC'GCA Cuts at: 3667 3667 Size: 5161 HaeII r_GCGC'y Cuts at: 1678 4308 1678 Size: 2630 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3802 3834 3876 3908 1373 Size: 32 135 32 2230 32 42 32 2626 Fragments arranged by size: 2626 2230 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2401 2505 2751 669 Size: 988 281 463 104 246 3079 Fragments arranged by size: 3079 988 463 281 246 104 HindIII A'AGCT_T Cuts at: 748 748 Size: 5161 HpaI GTT'AAC Cuts at: 2751 2751 Size: 5161 KpnI G_GTAC'C Cuts at: 2486 2486 Size: 5161 MfeI C'AATT_G Cuts at: 2760 2760 Size: 5161 MluI A'CGCG_T Cuts at: 2184 5037 2184 Size: 2853 2308 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4275 4459 1305 Size: 2970 184 2007 Fragments arranged by size: 2970 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2180 2369 10 Size: 54 1425 343 348 189 2802 Fragments arranged by size: 2802 1425 348 343 189 54 MslI CAynn'nnrTG Cuts at: 518 1166 2114 2390 3119 3478 3637 518 Size: 648 948 276 729 359 159 2042 Fragments arranged by size: 2042 948 729 648 359 276 159 MspA1I CmG'CkG Cuts at: 1606 2088 2194 2577 3254 4402 4647 1606 Size: 482 106 383 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 482 383 245 106 NaeI GCC'GGC Cuts at: 2082 2082 Size: 5161 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3670 Fragments arranged by size: 3670 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5161 NgoMIV G'CCGG_C Cuts at: 2080 2080 Size: 5161 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5161 NotI GC'GGCC_GC Cuts at: 2608 2608 Size: 5161 NspI r_CATG'y Cuts at: 1132 1229 2158 2519 4064 1132 Size: 97 929 361 1545 2229 Fragments arranged by size: 2229 1545 929 361 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5161 PciI A'CATG_T Cuts at: 4060 4060 Size: 5161 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5161 PmeI GTTT'AAAC Cuts at: 2465 2465 Size: 5161 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3230 3262 4743 4775 3230 Size: 32 1481 32 3616 Fragments arranged by size: 3616 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5161 PsiI TTA'TAA Cuts at: 2731 2731 Size: 5161 PspOMI G'GGCC_C Cuts at: 2105 2215 2105 Size: 110 5051 PstI C_TGCA'G Cuts at: 830 2277 2513 830 Size: 1447 236 3478 Fragments arranged by size: 3478 1447 236 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5161 PvuII CAG'CTG Cuts at: 2194 2194 Size: 5161 SacI G_AGCT'C Cuts at: 2034 2034 Size: 5161 SalI G'TCGA_C Cuts at: 1936 2503 1936 Size: 567 4594 SapI GCTCTTCn'nnn_ Cuts at: 2132 2132 Size: 5161 SbfI CC_TGCA'GG Cuts at: 2513 2513 Size: 5161 ScaI AGT'ACT Cuts at: 3409 5121 3409 Size: 1712 3449 SfcI C'TryA_G Cuts at: 826 1045 2273 2509 3644 4325 4516 826 Size: 219 1228 236 1135 681 191 1471 Fragments arranged by size: 1471 1228 1135 681 236 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5161 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5161 SmaI CCC'GGG Cuts at: 1872 2488 1872 Size: 616 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3265 4166 4428 820 Size: 197 926 1322 901 262 1553 Fragments arranged by size: 1553 1322 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2952 493 Size: 2459 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5161 SphI G_CATG'C Cuts at: 1132 2158 2519 1132 Size: 1026 361 3774 Fragments arranged by size: 3774 1026 361 SspI AAT'ATT Cuts at: 5 52 3085 5 Size: 47 3033 2081 Fragments arranged by size: 3033 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5161 StyI C'CwwG_G Cuts at: 513 1065 2004 2600 513 Size: 552 939 596 3074 Fragments arranged by size: 3074 939 596 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3188 3205 3358 3543 3988 325 Size: 1255 1608 17 153 185 445 1498 Fragments arranged by size: 1608 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3407 5119 96 Size: 274 80 33 51 1443 1430 1712 138 Fragments arranged by size: 1712 1443 1430 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5121 5153 5121 Size: 32 5129 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5161 XbaI T'CTAG_A Cuts at: 2497 2497 Size: 5161 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5161 XmaI C'CCGG_G Cuts at: 1870 2486 1870 Size: 616 4545 XmnI GAAnn'nnTTC Cuts at: 3290 3290 Size: 5161 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2969 276 Size: 53 83 186 936 1435 2468 Fragments arranged by size: 2468 1435 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI