(Linear) (Six-base) MAPSORT of: KIEE6554.seq Check: 3912 from: 1 to: 456 KIEE6554.orf With 182 enzymes: SgfI * March 16, 2015 11:33 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 211 456 Size: 211 245 AflIII A'CryG_T Cuts at: 0 179 456 Size: 179 277 ApaI G_GGCC'C Cuts at: 0 104 214 456 Size: 104 110 242 Fragments arranged by size: 242 110 104 AvaI C'yCGr_G Cuts at: 0 6 456 Size: 6 450 BanI G'GyrC_C Cuts at: 0 444 456 Size: 444 12 BanII G_rGCy'C Cuts at: 0 29 104 214 456 Size: 29 75 110 242 Fragments arranged by size: 242 110 75 29 BglI GCCn_nnn'nGGC Cuts at: 0 76 456 Size: 76 380 Bme1580I G_kGCm'C Cuts at: 0 52 104 214 236 456 Size: 52 52 110 22 220 Fragments arranged by size: 220 110 52 52 22 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 111 456 Size: 111 345 BsaHI Gr'CG_yC Cuts at: 0 68 456 Size: 68 388 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 31 456 Size: 31 425 BseYI C'CCAG_C Cuts at: 0 83 185 214 449 456 Size: 83 102 29 235 7 Fragments arranged by size: 235 102 83 29 7 BsiHKAI G_wGCw'C Cuts at: 0 29 456 Size: 29 427 Bsp1286I G_dGCh'C Cuts at: 0 29 52 104 214 236 456 Size: 29 23 52 110 22 220 Fragments arranged by size: 220 110 52 29 23 22 BsrFI r'CCGG_y Cuts at: 0 75 456 Size: 75 381 BstXI CCAn_nnnn'nTGG Cuts at: 0 372 456 Size: 372 84 BtgI C'CryG_G Cuts at: 0 365 456 Size: 365 91 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 144 456 Size: 144 312 EaeI y'GGCC_r Cuts at: 0 173 326 362 456 Size: 173 153 36 94 Fragments arranged by size: 173 153 94 36 EarI CTCTTCn'nnn_ Cuts at: 0 127 456 Size: 127 329 EcoICRI GAG'CTC Cuts at: 0 27 456 Size: 27 429 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 111 211 456 Size: 111 100 245 Fragments arranged by size: 245 111 100 EcoO109I rG'GnC_Cy Cuts at: 0 210 211 456 Size: 210 1 245 Fragments arranged by size: 245 210 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 363 456 Size: 363 93 HincII GTy'rAC Cuts at: 0 396 456 Size: 396 60 MluI A'CGCG_T Cuts at: 0 179 456 Size: 179 277 MscI TGG'CCA Cuts at: 0 175 364 456 Size: 175 189 92 Fragments arranged by size: 189 175 92 MslI CAynn'nnrTG Cuts at: 0 109 385 456 Size: 109 276 71 Fragments arranged by size: 276 109 71 MspA1I CmG'CkG Cuts at: 0 83 189 456 Size: 83 106 267 Fragments arranged by size: 267 106 83 NaeI GCC'GGC Cuts at: 0 77 456 Size: 77 379 NgoMIV G'CCGG_C Cuts at: 0 75 456 Size: 75 381 NspI r_CATG'y Cuts at: 0 153 456 Size: 153 303 PspOMI G'GGCC_C Cuts at: 0 100 210 456 Size: 100 110 246 Fragments arranged by size: 246 110 100 PstI C_TGCA'G Cuts at: 0 272 456 Size: 272 184 PvuII CAG'CTG Cuts at: 0 189 456 Size: 189 267 SacI G_AGCT'C Cuts at: 0 29 456 Size: 29 427 SapI GCTCTTCn'nnn_ Cuts at: 0 127 456 Size: 127 329 SfcI C'TryA_G Cuts at: 0 268 456 Size: 268 188 SphI G_CATG'C Cuts at: 0 153 456 Size: 153 303 Enzymes that do cut: AcuI AflIII ApaI AvaI BanI BanII BglI Bme1580I BpmI BsaHI BseRI BseYI BsiHKAI Bsp1286I BsrFI BstXI BtgI BtgZI EaeI EarI EcoICRI Eco57MI EcoO109I FalI HincII MluI MscI MslI MspA1I NaeI NgoMIV NspI PspOMI PstI PvuII SacI SapI SfcI SphI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII DrdI EagI EciI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII Hin4I HindIII HpaI KasI KpnI MfeI MmeI NarI NcoI NdeI NheI NotI NruI NsiI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI