################################## # NO CUT ENZYMES ( 113 enzymes) # ################################## AatII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI AlwI ApoI AscI AseI AsnI AspEI AspI AviII AvrII BamHI BbeI BbvCI BcgI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsiWI BsmBI BspDI BspEI BspLU11I BsrBI BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstYI BstZ17I CelII ClaI CpoI DraIII DrdI Eam1105I EcoNI EcoRI EcoRV EcoT22I FseI FspI Hin1I HindIII HpaI KasI KspI MaeI MamI MfeI MflI MluI MroI MspA1I MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PinAI PmeI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI SwaI Tth111I XbaI XcmI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### Acc65I 35 GGTACC G^GTACC AccI 1422 GTCGAC GT^[AC][GT]AC Alw44I 784 GTGCAC G^TGCAC AlwNI 1111 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 859 CCTCAGG CC^T[ACGT]AGG ApaLI 784 GTGCAC G^TGCAC Asp718I 35 GGTACC G^GTACC BalI 740 TGGCCA TGG^CCA BbrPI 787 CACGTG CAC^GTG BciVI 315 GGATAC GTATCC(6/5) BglI 827 GCCGGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BseRI 1060 GAGGAG GAGGAG(10/8) BsiEI 388 CGGCCG CG[GA][CT]^CG BsrFI 386 ACCGGC [GA]^CCGG[CT] BstEII 941 GGTTACC G^GT[ACGT]ACC BstPI 941 GGTTACC G^GT[ACGT]ACC BstXI 731 CCAACGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 859 CCTCAGG CC^T[ACGT]AGG Cfr10I 386 ACCGGC [GA]^CCGG[CT] DraI 267 TTTAAA TTT^AAA EagI 388 CGGCCG C^GGCCG EclXI 388 CGGCCG C^GGCCG Eco52I 388 CGGCCG C^GGCCG Eco81I 859 CCTCAGG CC^T[ACGT]AGG EcoO65I 941 GGTTACC G^GT[ACGT]ACC FauI 867 CCCGC CCCGC(4/6) HphI 640 GGTGA GGTGA(8/7) KpnI 35 GGTACC GGTAC^C MluNI 740 TGGCCA TGG^CCA MscI 740 TGGCCA TGG^CCA MslI 795 CACACGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NspI 485 GCATGC [GA]CATG^[CT] PaeR7I 438 CTCGAG C^TCGAG PflMI 415 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 787 CACGTG CAC^GTG PmlI 787 CACGTG CAC^GTG PpuMI 308 AGGACCT [GA]G^G[AT]CC[CT] PshAI 287 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SalI 1422 GTCGAC G^TCGAC SphI 485 GCATGC GCATG^C TfiI 393 GATTC G^A[AT]TC Tsp509I 349 AATT ^AATT Van91I 415 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 438 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### Aor51HI 80 AGCGCT AGC^GCT 932 AGCGCT ApaI 748 GGGCCC GGGCC^C 864 GGGCCC Asp700I 711 GAAGCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1048 GAAGAGTTTC AspHI 382 GAGCAC G[AT]GC[AT]^C 784 GTGCAC BanI 35 GGTACC G^G[CT][GA]CC 835 GGCACC BbsI 126 GAAGAC GAAGAC(2/6) 956 GTCTTC BpmI 973 CTCCAG CTGGAG(16/14) 687 CTGGAG BpuAI 126 GAAGAC GAAGAC(2/6) 956 GTCTTC BsaAI 787 CACGTG [CT]AC^GT[GA] 1397 TACGTG BsiHKAI 382 GAGCAC G[AT]GC[AT]^C 784 GTGCAC BsmAI 294 GTCTC GTCTC(1/5) 1227 GTCTC BsmI 575 GAATGC GAATGC(1/-1) 1340 GCATTC Bsp1407I 962 TGTACA T^GTACA 1022 TGTACA BspHI 185 TCATGA T^CATGA 1214 TCATGA BspMI 152 ACCTGC ACCTGC(4/8) 992 ACCTGC BsrGI 962 TGTACA T^GTACA 1022 TGTACA BstF5I 1137 GGATG GGATG(2/0) 1242 GGATG DsaI 39 CCACGG C^C[GA][CT]GG 677 CCGTGG EaeI 388 CGGCCG [CT]^GGCC[GA] 740 TGGCCA Eco47III 80 AGCGCT AGC^GCT 932 AGCGCT EcoT14I 912 CCAAGG C^C[AT][AT]GG 1199 CCAAGG FokI 1137 GGATG GGATG(9/13) 1242 GGATG HaeII 80 AGCGCT [GA]GCGC^[CT] 932 AGCGCT HgaI 86 GCGTC GACGC(5/10) 1184 GCGTC HincII 1375 GTCAAC GT[CT]^[GA]AC 1422 GTCGAC HindII 1375 GTCAAC GT[CT]^[GA]AC 1422 GTCGAC MaeII 788 ACGT A^CGT 1398 ACGT RcaI 185 TCATGA T^CATGA 1214 TCATGA SfaNI 664 GATGC GCATC(5/9) 1243 GATGC SspBI 962 TGTACA T^GTACA 1022 TGTACA StuI 1008 AGGCCT AGG^CCT 1261 AGGCCT StyI 912 CCAAGG C^C[AT][AT]GG 1199 CCAAGG Tsp45I 302 GTGAC ^GT[GC]AC 448 GTCAC XmnI 711 GAAGCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1048 GAAGAGTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AccII AciI AfaI AluI AvaI AvaII BanII BbvI BclI BcnI BmyI BsaJI BsgI BsiYI BslI BsmFI BsoBI Bsp1286I BsrI BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII FbaI Fnu4HI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI Ksp632I MaeIII MboI MboII MnlI MseI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PstI RsaI Sau3AI Sau96I ScrFI SfcI SmlI TaqI Tru9I TseI TspRI TthHB8I