(Circular) (Six-base) MAPSORT of: pFN21AE3213.seq Check: 618 from: 1 to: 6178 pFN21AE3213 6178 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2166 2166 Size: 6178 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3988 278 Size: 53 83 186 936 2452 2468 Fragments arranged by size: 2468 2452 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3428 3521 1937 Size: 1491 93 4594 Fragments arranged by size: 4594 1491 93 Acc65I G'GTAC_C Cuts at: 2040 3499 2040 Size: 1459 4719 AclI AA'CG_TT Cuts at: 1563 4305 4678 1563 Size: 2742 373 3063 Fragments arranged by size: 3063 2742 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2129 2735 4241 5625 5893 1632 Size: 497 606 1506 1384 268 1917 Fragments arranged by size: 1917 1506 1384 606 497 268 AfeI AGC'GCT Cuts at: 2087 2939 2087 Size: 852 5326 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5981 AflIII A'CryG_T Cuts at: 1203 5077 6054 1203 Size: 3874 977 1327 Fragments arranged by size: 3874 1327 977 AgeI A'CCGG_T Cuts at: 5009 5009 Size: 6178 AhdI GACnn_n'nnGTC Cuts at: 4907 4907 Size: 6178 AleI CACnn'nnGTG Cuts at: 1166 2804 1166 Size: 1638 4540 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6178 AlwNI CAG_nnn'CTG Cuts at: 3121 3921 5493 3121 Size: 800 1572 3806 Fragments arranged by size: 3806 1572 800 ApaI G_GGCC'C Cuts at: 2757 2873 2757 Size: 116 6062 ApaLI G'TGCA_C Cuts at: 2789 4235 5391 2789 Size: 1446 1156 3576 Fragments arranged by size: 3576 1446 1156 ApoI r'AATT_y Cuts at: 1493 3487 3713 3865 5974 1493 Size: 1994 226 152 2109 1697 Fragments arranged by size: 2109 1994 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4732 160 Size: 872 3700 1606 Fragments arranged by size: 3700 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6178 AvaI C'yCGr_G Cuts at: 1870 1943 2443 2858 2941 3062 3503 1870 Size: 73 500 415 83 121 441 4545 Fragments arranged by size: 4545 500 441 415 121 83 73 BamHI G'GATC_C Cuts at: 3508 3875 3508 Size: 367 5811 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2040 2840 3499 4954 618 Size: 325 217 627 253 800 659 1455 1842 Fragments arranged by size: 1842 1455 800 659 627 325 253 217 BanII G_rGCy'C Cuts at: 1391 2681 2757 2859 2873 3497 1391 Size: 1290 76 102 14 624 4072 Fragments arranged by size: 4072 1290 624 102 76 14 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2138 2954 928 Size: 971 239 816 4152 Fragments arranged by size: 4152 971 816 239 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4369 4403 1903 Size: 34 2432 34 3678 Fragments arranged by size: 3678 2432 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2314 4070 5280 2314 Size: 1756 1210 3212 Fragments arranged by size: 3212 1756 1210 BclI T'GATC_A Cuts at: 1548 2775 2796 3259 1548 Size: 1227 21 463 4467 Fragments arranged by size: 4467 1227 463 21 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2838 4789 136 Size: 107 122 71 631 605 1166 1951 1525 Fragments arranged by size: 1951 1525 1166 631 605 122 107 71 BglII A'GATC_T Cuts at: 6173 6173 Size: 6178 BlpI GC'TnA_GC Cuts at: 3595 3595 Size: 6178 Bme1580I G_kGCm'C Cuts at: 1790 2757 2793 2873 4239 5395 1790 Size: 967 36 80 1366 1156 2573 Fragments arranged by size: 2573 1366 1156 967 80 36 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6178 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2526 3909 4867 Size: 416 53 83 399 24 1109 1383 958 Cuts at: 4867 442 Size: 1753 Fragments arranged by size: 1753 1383 1109 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6178 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2713 2963 4838 1239 Size: 1474 250 1875 2579 Fragments arranged by size: 2579 1875 1474 250 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2489 3314 4303 5168 5466 2489 Size: 825 989 865 298 3201 Fragments arranged by size: 3201 989 865 825 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4841 882 Size: 617 3342 2219 Fragments arranged by size: 3342 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2794 3404 3969 493 Size: 711 1590 610 565 2702 Fragments arranged by size: 2702 1590 711 610 565 BsaBI GATnn'nnATC Cuts at: 6172 6172 Size: 6178 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3985 4367 5002 275 Size: 53 83 186 936 2452 382 635 1451 Fragments arranged by size: 2452 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4611 5009 5283 5430 5841 4611 Size: 398 274 147 411 4948 Fragments arranged by size: 4948 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5953 Fragments arranged by size: 5953 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3080 1186 Size: 1894 4284 BseYI C'CCAG_C Cuts at: 5381 5877 5381 Size: 496 5682 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2746 3005 3197 1163 Size: 573 1010 259 192 4144 Fragments arranged by size: 4144 1010 573 259 192 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2396 3628 4389 5417 1540 Size: 275 186 395 1232 761 1028 2301 Fragments arranged by size: 2301 1232 1028 761 395 275 186 BsiHKAI G_wGCw'C Cuts at: 2391 2793 4239 4324 5395 2391 Size: 402 1446 85 1071 3174 Fragments arranged by size: 3174 1446 1071 402 85 BsmI GAATG_Cn' Cuts at: 2586 3345 3689 3782 2586 Size: 759 344 93 4982 Fragments arranged by size: 4982 759 344 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2391 2681 2757 2793 2859 2873 3497 Size: 399 601 290 76 36 66 14 624 Cuts at: 3497 4239 4324 5395 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 624 601 399 290 85 76 66 36 14 BspEI T'CCGG_A Cuts at: 5841 5841 Size: 6178 BspHI T'CATG_A Cuts at: 2190 3219 4067 5797 2190 Size: 1029 848 1730 2571 Fragments arranged by size: 2571 1730 1029 848 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2166 3006 3533 844 Size: 1322 840 527 3489 Fragments arranged by size: 3489 1322 840 527 BsrBI CCG'CTC Cuts at: 1394 1823 4065 1394 Size: 429 2242 3507 Fragments arranged by size: 3507 2242 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4673 4847 65 Size: 1183 3425 174 1396 Fragments arranged by size: 3425 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2391 4822 5009 1700 Size: 110 581 2431 187 2869 Fragments arranged by size: 2869 2431 581 187 110 BsrGI T'GTAC_A Cuts at: 96 2967 3027 96 Size: 2871 60 3247 Fragments arranged by size: 3247 2871 60 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6178 BssSI C'ACGA_G Cuts at: 4238 5250 4238 Size: 1012 5166 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6178 BstBI TT'CG_AA Cuts at: 4982 4982 Size: 6178 BstEII G'GTnAC_C Cuts at: 1184 2946 1184 Size: 1762 4416 BstXI CCAn_nnnn'nTGG Cuts at: 2743 2743 Size: 6178 BstYI r'GATC_y Cuts at: 1972 3508 3875 4260 4277 5729 5815 5827 6173 Size: 1536 367 385 17 1452 86 12 346 Cuts at: 6173 1972 Size: 1977 Fragments arranged by size: 1977 1536 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2865 2865 Size: 6178 BtgI C'CryG_G Cuts at: 513 1065 2004 2044 2682 513 Size: 552 939 40 638 4009 Fragments arranged by size: 4009 939 638 552 40 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5439 Fragments arranged by size: 5439 683 56 BtsI GCAGTG_nn' Cuts at: 2758 3694 4488 4508 2758 Size: 936 794 20 4428 Fragments arranged by size: 4428 936 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6178 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6143 DraI TTT'AAA Cuts at: 2274 3482 2274 Size: 1208 4970 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5185 809 Size: 507 3869 1802 Fragments arranged by size: 3869 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2393 2745 3625 Size: 54 1425 50 275 18 563 352 880 Cuts at: 3625 4514 6143 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 880 563 352 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2393 3625 1537 Size: 275 581 1232 4090 Fragments arranged by size: 4090 1232 581 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2004 2136 2591 3048 4108 1353 Size: 651 132 455 457 1060 3423 Fragments arranged by size: 3423 1060 651 457 455 132 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4765 5139 5285 387 Size: 1064 138 3176 374 146 1280 Fragments arranged by size: 3176 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2129 2713 2735 2963 4241 4838 5625 Size: 393 497 584 22 228 1278 597 787 Cuts at: 5625 5893 1239 Size: 268 1524 Fragments arranged by size: 1524 1278 787 597 584 497 393 268 228 22 EcoO109I rG'GnC_Cy Cuts at: 2314 2754 2869 2314 Size: 440 115 5623 Fragments arranged by size: 5623 440 115 EcoRI G'AATT_C Cuts at: 3487 3865 3487 Size: 378 5800 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2740 2740 Size: 6178 FspI TGC'GCA Cuts at: 4684 4684 Size: 6178 HaeII r_GCGC'y Cuts at: 1678 2089 2941 5325 1678 Size: 411 852 2384 2531 Fragments arranged by size: 2531 2384 852 411 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2546 2578 3239 3271 3415 Size: 32 135 32 974 32 661 32 144 Cuts at: 3415 3447 4819 4851 4893 4925 1373 Size: 32 1372 32 42 32 2626 Fragments arranged by size: 2626 1372 974 661 144 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3382 3429 3522 3768 669 Size: 988 281 1444 47 93 246 3079 Fragments arranged by size: 3079 1444 988 281 246 93 47 HindIII A'AGCT_T Cuts at: 748 748 Size: 6178 HpaI GTT'AAC Cuts at: 3768 3768 Size: 6178 KpnI G_GTAC'C Cuts at: 2044 3503 2044 Size: 1459 4719 MfeI C'AATT_G Cuts at: 3777 3777 Size: 6178 MluI A'CGCG_T Cuts at: 6054 6054 Size: 6178 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2760 2973 5292 5476 1305 Size: 1455 213 2319 184 2007 Fragments arranged by size: 2319 2007 1455 213 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2747 10 Size: 54 1425 343 915 3441 Fragments arranged by size: 3441 1425 915 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2804 4136 4495 4654 518 Size: 648 1638 1332 359 159 2042 Fragments arranged by size: 2042 1638 1332 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3594 4271 5419 5664 1606 Size: 1988 677 1148 245 2120 Fragments arranged by size: 2120 1988 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4687 Fragments arranged by size: 4687 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6178 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6178 NotI GC'GGCC_GC Cuts at: 3625 3625 Size: 6178 NspI r_CATG'y Cuts at: 1132 1229 2494 3536 5081 1132 Size: 97 1265 1042 1545 2229 Fragments arranged by size: 2229 1545 1265 1042 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6178 PciI A'CATG_T Cuts at: 5077 5077 Size: 6178 PflMI CCAn_nnn'nTGG Cuts at: 1108 2426 1108 Size: 1318 4860 PmeI GTTT'AAAC Cuts at: 3482 3482 Size: 6178 PmlI CAC'GTG Cuts at: 2794 2794 Size: 6178 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4247 4279 5760 5792 4247 Size: 32 1481 32 4633 Fragments arranged by size: 4633 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2314 2314 Size: 6178 PshAI GACnn'nnGTC Cuts at: 1613 2296 1613 Size: 683 5495 PsiI TTA'TAA Cuts at: 3748 3748 Size: 6178 PspOMI G'GGCC_C Cuts at: 2753 2869 2753 Size: 116 6062 PstI C_TGCA'G Cuts at: 830 2163 2610 3246 3530 830 Size: 1333 447 636 284 3478 Fragments arranged by size: 3478 1333 636 447 284 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6178 SalI G'TCGA_C Cuts at: 1936 3427 3520 1936 Size: 1491 93 4594 Fragments arranged by size: 4594 1491 93 SbfI CC_TGCA'GG Cuts at: 3530 3530 Size: 6178 ScaI AGT'ACT Cuts at: 4426 6138 4426 Size: 1712 4466 SfcI C'TryA_G Cuts at: 826 1045 2159 2606 3242 3526 4661 5342 5533 Size: 219 1114 447 636 284 1135 681 191 Cuts at: 5533 826 Size: 1471 Fragments arranged by size: 1471 1135 1114 681 636 447 284 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6178 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6178 SmaI CCC'GGG Cuts at: 1872 3505 1872 Size: 1633 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2443 2504 3329 4282 5183 5445 Size: 197 926 500 61 825 953 901 262 Cuts at: 5445 820 Size: 1553 Fragments arranged by size: 1553 953 926 901 825 500 262 197 61 SnaBI TAC'GTA Cuts at: 493 3969 493 Size: 3476 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6178 SphI G_CATG'C Cuts at: 1132 2494 3536 1132 Size: 1362 1042 3774 Fragments arranged by size: 3774 1362 1042 SspI AAT'ATT Cuts at: 5 52 4102 5 Size: 47 4050 2081 Fragments arranged by size: 4050 2081 47 StuI AGG'CCT Cuts at: 1517 3015 3268 1517 Size: 1498 253 4427 Fragments arranged by size: 4427 1498 253 StyI C'CwwG_G Cuts at: 513 1065 2004 2917 3204 3617 513 Size: 552 939 913 287 413 3074 Fragments arranged by size: 3074 939 913 552 413 287 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4205 4222 4375 4560 5005 325 Size: 1255 2625 17 153 185 445 1498 Fragments arranged by size: 2625 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2967 3027 4424 Size: 274 80 33 51 1443 990 60 1397 Cuts at: 4424 6136 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1397 990 274 138 80 60 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2833 2865 6138 6170 2833 Size: 32 3273 32 2841 Fragments arranged by size: 3273 2841 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6178 XbaI T'CTAG_A Cuts at: 3514 3514 Size: 6178 XhoI C'TCGA_G Cuts at: 1943 2443 1943 Size: 500 5678 XmaI C'CCGG_G Cuts at: 1870 3503 1870 Size: 1633 4545 XmnI GAAnn'nnTTC Cuts at: 2720 3057 4307 2720 Size: 337 1250 4591 Fragments arranged by size: 4591 1250 337 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3986 276 Size: 53 83 186 936 2452 2468 Fragments arranged by size: 2468 2452 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI