(Linear) (Six-base) MAPSORT of: KIEE3213.seq Check: 1348 from: 1 to: 1473 KIEE3213.orf With 182 enzymes: SgfI * December 27, 2011 16:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 161 1473 Size: 161 1312 AccI GT'mk_AC Cuts at: 0 1423 1473 Size: 1423 50 Acc65I G'GTAC_C Cuts at: 0 35 1473 Size: 35 1438 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 124 730 1473 Size: 124 606 743 Fragments arranged by size: 743 606 124 AfeI AGC'GCT Cuts at: 0 82 934 1473 Size: 82 852 539 Fragments arranged by size: 852 539 82 AleI CACnn'nnGTG Cuts at: 0 799 1473 Size: 799 674 AlwNI CAG_nnn'CTG Cuts at: 0 1116 1473 Size: 1116 357 ApaI G_GGCC'C Cuts at: 0 752 868 1473 Size: 752 116 605 Fragments arranged by size: 752 605 116 ApaLI G'TGCA_C Cuts at: 0 784 1473 Size: 784 689 AvaI C'yCGr_G Cuts at: 0 438 853 936 1057 1473 Size: 438 415 83 121 416 Fragments arranged by size: 438 416 415 121 83 BanI G'GyrC_C Cuts at: 0 35 835 1473 Size: 35 800 638 Fragments arranged by size: 800 638 35 BanII G_rGCy'C Cuts at: 0 676 752 854 868 1473 Size: 676 76 102 14 605 Fragments arranged by size: 676 605 102 76 14 BbsI GAAGACnn'nnnn_ Cuts at: 0 133 949 1473 Size: 133 816 524 Fragments arranged by size: 816 524 133 BciVI GTATCCnnnnn_n' Cuts at: 0 309 1473 Size: 309 1164 BclI T'GATC_A Cuts at: 0 770 791 1254 1473 Size: 770 21 463 219 Fragments arranged by size: 770 463 219 21 BglI GCCn_nnn'nGGC Cuts at: 0 833 1473 Size: 833 640 Bme1580I G_kGCm'C Cuts at: 0 752 788 868 1473 Size: 752 36 80 605 Fragments arranged by size: 752 605 80 36 BmrI ACTGGGnnnn_n' Cuts at: 0 521 1473 Size: 521 952 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 708 958 1473 Size: 708 250 515 Fragments arranged by size: 708 515 250 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 484 1309 1473 Size: 484 825 164 Fragments arranged by size: 825 484 164 BsaAI yAC'GTr Cuts at: 0 789 1399 1473 Size: 789 610 74 Fragments arranged by size: 789 610 74 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1075 1473 Size: 1075 398 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 741 1000 1192 1473 Size: 741 259 192 281 Fragments arranged by size: 741 281 259 192 BsiEI CG_ry'CG Cuts at: 0 391 1473 Size: 391 1082 BsiHKAI G_wGCw'C Cuts at: 0 386 788 1473 Size: 386 402 685 Fragments arranged by size: 685 402 386 BsmI GAATG_Cn' Cuts at: 0 581 1340 1473 Size: 581 759 133 Fragments arranged by size: 759 581 133 Bsp1286I G_dGCh'C Cuts at: 0 386 676 752 788 854 868 1473 Size: 386 290 76 36 66 14 605 Fragments arranged by size: 605 386 290 76 66 36 14 BspHI T'CATG_A Cuts at: 0 185 1214 1473 Size: 185 1029 259 Fragments arranged by size: 1029 259 185 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 161 1001 1473 Size: 161 840 472 Fragments arranged by size: 840 472 161 BsrFI r'CCGG_y Cuts at: 0 386 1473 Size: 386 1087 BsrGI T'GTAC_A Cuts at: 0 962 1022 1473 Size: 962 60 451 Fragments arranged by size: 962 451 60 BstEII G'GTnAC_C Cuts at: 0 941 1473 Size: 941 532 BstXI CCAn_nnnn'nTGG Cuts at: 0 738 1473 Size: 738 735 Bsu36I CC'TnA_GG Cuts at: 0 860 1473 Size: 860 613 BtgI C'CryG_G Cuts at: 0 39 677 1473 Size: 39 638 796 Fragments arranged by size: 796 638 39 BtsI GCAGTG_nn' Cuts at: 0 753 1473 Size: 753 720 DraI TTT'AAA Cuts at: 0 269 1473 Size: 269 1204 EaeI y'GGCC_r Cuts at: 0 388 740 1473 Size: 388 352 733 Fragments arranged by size: 733 388 352 EagI C'GGCC_G Cuts at: 0 388 1473 Size: 388 1085 EarI CTCTTCn'nnn_ Cuts at: 0 131 586 1043 1473 Size: 131 455 457 430 Fragments arranged by size: 457 455 430 131 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 124 708 730 958 1473 Size: 124 584 22 228 515 Fragments arranged by size: 584 515 228 124 22 EcoO109I rG'GnC_Cy Cuts at: 0 309 749 864 1473 Size: 309 440 115 609 Fragments arranged by size: 609 440 309 115 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 735 1473 Size: 735 738 HaeII r_GCGC'y Cuts at: 0 84 936 1473 Size: 84 852 537 Fragments arranged by size: 852 537 84 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 541 573 1234 1266 1410 1442 1473 Size: 541 32 661 32 144 32 31 Fragments arranged by size: 661 541 144 32 32 32 31 HincII GTy'rAC Cuts at: 0 1377 1424 1473 Size: 1377 47 49 Fragments arranged by size: 1377 49 47 KpnI G_GTAC'C Cuts at: 0 39 1473 Size: 39 1434 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 755 968 1473 Size: 755 213 505 Fragments arranged by size: 755 505 213 MscI TGG'CCA Cuts at: 0 742 1473 Size: 742 731 MslI CAynn'nnrTG Cuts at: 0 799 1473 Size: 799 674 NspI r_CATG'y Cuts at: 0 489 1473 Size: 489 984 PflMI CCAn_nnn'nTGG Cuts at: 0 421 1473 Size: 421 1052 PmlI CAC'GTG Cuts at: 0 789 1473 Size: 789 684 PpuMI rG'GwC_Cy Cuts at: 0 309 1473 Size: 309 1164 PshAI GACnn'nnGTC Cuts at: 0 291 1473 Size: 291 1182 PspOMI G'GGCC_C Cuts at: 0 748 864 1473 Size: 748 116 609 Fragments arranged by size: 748 609 116 PstI C_TGCA'G Cuts at: 0 158 605 1241 1473 Size: 158 447 636 232 Fragments arranged by size: 636 447 232 158 SalI G'TCGA_C Cuts at: 0 1422 1473 Size: 1422 51 SfcI C'TryA_G Cuts at: 0 154 601 1237 1473 Size: 154 447 636 236 Fragments arranged by size: 636 447 236 154 SmlI C'TyrA_G Cuts at: 0 438 499 1324 1473 Size: 438 61 825 149 Fragments arranged by size: 825 438 149 61 SphI G_CATG'C Cuts at: 0 489 1473 Size: 489 984 StuI AGG'CCT Cuts at: 0 1010 1263 1473 Size: 1010 253 210 Fragments arranged by size: 1010 253 210 StyI C'CwwG_G Cuts at: 0 912 1199 1473 Size: 912 287 274 Fragments arranged by size: 912 287 274 TatI w'GTAC_w Cuts at: 0 962 1022 1473 Size: 962 60 451 Fragments arranged by size: 962 451 60 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 828 860 1473 Size: 828 32 613 Fragments arranged by size: 828 613 32 XhoI C'TCGA_G Cuts at: 0 438 1473 Size: 438 1035 XmnI GAAnn'nnTTC Cuts at: 0 715 1052 1473 Size: 715 337 421 Fragments arranged by size: 715 421 337 Enzymes that do cut: AarI AccI Acc65I AcuI AfeI AleI AlwNI ApaI ApaLI AvaI BanI BanII BbsI BciVI BclI BglI Bme1580I BmrI BpmI BpuEI BsaAI BseRI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrFI BsrGI BstEII BstXI Bsu36I BtgI BtsI DraI EaeI EagI EarI Eco57MI EcoO109I FalI HaeII Hin4I HincII KpnI MmeI MscI MslI NspI PflMI PmlI PpuMI PshAI PspOMI PstI SalI SfcI SmlI SphI StuI StyI TatI TstI XhoI XmnI Enzymes that do not cut: AatII AclI AflII AflIII AgeI AhdI AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BfrBI BglII BlpI BmgBI BmtI BplI Bpu10I BsaI BsaBI BsaHI BsaWI BsaXI BseYI BsiWI BsmBI BspEI BsrBI BsrDI BssHII BssSI BstAPI BstBI BstYI BstZ17I BtgZI ClaI CspCI DraIII DrdI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HindIII HpaI KasI MfeI MluI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PpiI PsiI PspXI PsrI PvuI PvuII RsrII SacI SacII SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TaqII Tth111I XbaI XcmI XmaI ZraI