################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## AccI AccII AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BamHI BciVI BclI BfrI BlnI BlpI Bpu1102I BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmI BspDI BspEI BspHI BspLU11I BsrBI BsrDI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I CelII ClaI CpoI DraI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI Ksp632I KspI MaeIII MamI MfeI MluI MroI MspA1I MunI MvnI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI SbfI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tsp45I Tth111I XbaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AatII 766 GACGTC GACGT^C Acc65I 503 GGTACC G^GTACC ApaI 946 GGGCCC GGGCC^C Asp718I 503 GGTACC G^GTACC BalI 491 TGGCCA TGG^CCA BbeI 37 GGCGCC GGCGC^C BbsI 52 GTCTTC GAAGAC(2/6) BbvCI 907 CCTCAGC CCTCAGC(-5/-2) BcgI 81 CGAGATAGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 928 GCCCCCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 716 AGATCT A^GATCT BpuAI 52 GTCTTC GAAGAC(2/6) BsmBI 768 CGTCTC CGTCTC(1/5) Bsp1407I 369 TGTACA T^GTACA BsrFI 977 ACCGGC [GA]^CCGG[CT] BsrGI 369 TGTACA T^GTACA BstAPI 324 GCAGATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Cfr10I 977 ACCGGC [GA]^CCGG[CT] DrdI 544 GACCAGGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 42 CCACGG C^C[GA][CT]GG HphI 819 TCACC GGTGA(8/7) KasI 37 GGCGCC G^GCGCC KpnI 503 GGTACC GGTAC^C MluNI 491 TGGCCA TGG^CCA MscI 491 TGGCCA TGG^CCA MslI 955 CACATCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 37 GGCGCC GG^CGCC PaeR7I 446 CTCGAG C^TCGAG SfoI 37 GGCGCC GGC^GCC SmaI 612 CCCGGG CCC^GGG SspBI 369 TGTACA T^GTACA Tsp509I 800 AATT ^AATT TspRI 693 CACTG CA[GC]TG[ACGT][ACGT]^ XhoI 446 CTCGAG C^TCGAG XmaCI 612 CCCGGG C^CCGGG XmaI 612 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AlwI 258 GGATC GGATC(4/5) 786 GGATC AlwNI 139 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG 325 CAGATGCTG AocI 108 CCTAAGG CC^T[ACGT]AGG 410 CCTCAGG AspHI 517 GAGCTC G[AT]GC[AT]^C 688 GAGCTC BbrPI 67 CACGTG CAC^GTG 970 CACGTG BcnI 612 CCCGG CC^[GC]GG 613 CCGGG BfaI 295 CTAG C^TAG 916 CTAG BpmI 748 CTGGAG CTGGAG(16/14) 1030 CTGGAG BsaAI 67 CACGTG [CT]AC^GT[GA] 970 CACGTG BsiHKAI 517 GAGCTC G[AT]GC[AT]^C 688 GAGCTC BsmAI 73 GAGAC GTCTC(1/5) 769 GTCTC BstXI 62 CCAAGCACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 740 CCATGCCCCTGG Bsu36I 108 CCTAAGG CC^T[ACGT]AGG 410 CCTCAGG Eco81I 108 CCTAAGG CC^T[ACGT]AGG 410 CCTCAGG EcoNI 93 CCTCAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 747 CCTGGAGGAGG EcoT14I 839 CCAAGG C^C[AT][AT]GG 932 CCAAGG FauI 533 CCCGC CCCGC(4/6) 147 GCGGG HaeII 37 GGCGCC [GA]GCGC^[CT] 406 AGCGCC HgaI 551 GCGTC GACGC(5/10) 912 GCGTC MaeI 295 CTAG C^TAG 916 CTAG MboII 53 TCTTC GAAGA(8/7) 863 TCTTC MseI 155 TTAA T^TAA 725 TTAA NciI 612 CCCGG CC^[GC]GG 613 CCGGG PleI 449 GAGTC GAGTC(4/5) 991 GAGTC PmaCI 67 CACGTG CAC^GTG 970 CACGTG PmlI 67 CACGTG CAC^GTG 970 CACGTG PstI 163 CTGCAG CTGCA^G 322 CTGCAG SacI 517 GAGCTC GAGCT^C 688 GAGCTC ScaI 8 AGTACT AGT^ACT 857 AGTACT SexAI 485 ACCAGGT A^CC[AT]GGT 496 ACCAGGT SfcI 163 CTGCAG C^T[GA][CT]AG 322 CTGCAG StyI 839 CCAAGG C^C[AT][AT]GG 932 CCAAGG TaqI 447 TCGA T^CGA 554 TCGA TfiI 903 GATTC G^A[AT]TC 1014 GATTC Tru9I 155 TTAA T^TAA 725 TTAA TthHB8I 447 TCGA T^CGA 554 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 65 enzymes) # ######################################### AciI AcyI AfaI AluI AvaI AvaII BanI BanII BbvI BmyI BsaHI BsaJI BseRI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII EaeI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII ItaI MaeII MboI MflI MnlI MspI MvaI MwoI NdeII NlaIII NlaIV PflMI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI TseI Van91I XhoII