(Circular) (Six-base) MAPSORT of: pFN21AE3413.seq Check: 5604 from: 1 to: 5743 pFN21AE3413 5743 bp With 182 enzymes: SgfI * March 28, 2012 12:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2728 2728 Size: 5743 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2775 3553 278 Size: 53 83 186 936 1239 778 2468 Fragments arranged by size: 2468 1239 936 778 186 83 53 AccI GT'mk_AC Cuts at: 1937 3086 1937 Size: 1149 4594 Acc65I G'GTAC_C Cuts at: 2508 3064 2508 Size: 556 5187 AclI AA'CG_TT Cuts at: 1563 3870 4243 1563 Size: 2307 373 3063 Fragments arranged by size: 3063 2307 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2225 3806 5190 5458 1632 Size: 593 1581 1384 268 1917 Fragments arranged by size: 1917 1581 1384 593 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5546 AflIII A'CryG_T Cuts at: 1203 4642 5619 1203 Size: 3439 977 1327 Fragments arranged by size: 3439 1327 977 AgeI A'CCGG_T Cuts at: 4574 4574 Size: 5743 AhdI GACnn_n'nnGTC Cuts at: 4472 4472 Size: 5743 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5743 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5743 AlwNI CAG_nnn'CTG Cuts at: 2149 2335 3486 5058 2149 Size: 186 1151 1572 2834 Fragments arranged by size: 2834 1572 1151 186 ApaI G_GGCC'C Cuts at: 2955 2955 Size: 5743 ApaLI G'TGCA_C Cuts at: 3800 4956 3800 Size: 1156 4587 ApoI r'AATT_y Cuts at: 1493 3052 3278 3430 5539 1493 Size: 1559 226 152 2109 1697 Fragments arranged by size: 2109 1697 1559 226 152 AseI AT'TA_AT Cuts at: 160 1032 4297 160 Size: 872 3265 1606 Fragments arranged by size: 3265 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5743 AvaI C'yCGr_G Cuts at: 1870 1943 2451 2519 2617 3068 1870 Size: 73 508 68 98 451 4545 Fragments arranged by size: 4545 508 451 98 73 68 BamHI G'GATC_C Cuts at: 3073 3440 3073 Size: 367 5376 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2042 2508 2534 2666 2740 Size: 325 217 627 255 466 26 132 74 Cuts at: 2740 3064 4519 618 Size: 324 1455 1842 Fragments arranged by size: 1842 1455 627 466 325 324 255 217 132 74 26 BanII G_rGCy'C Cuts at: 1391 2518 2526 2697 2955 3062 1391 Size: 1127 8 171 258 107 4072 Fragments arranged by size: 4072 1127 258 171 107 8 BbeI G_GCGC'C Cuts at: 2046 2046 Size: 5743 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2050 928 Size: 971 151 4621 Fragments arranged by size: 4621 971 151 BbvCI CC'TCA_GC Cuts at: 2913 2913 Size: 5743 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2075 2109 3934 3968 1903 Size: 34 138 34 1825 34 3678 Fragments arranged by size: 3678 1825 138 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3635 4845 3635 Size: 1210 4533 BclI T'GATC_A Cuts at: 1548 1548 Size: 5743 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2939 4354 136 Size: 107 122 71 631 605 1267 1415 1525 Fragments arranged by size: 1525 1415 1267 631 605 122 107 71 BglII A'GATC_T Cuts at: 2721 5738 2721 Size: 3017 2726 BlpI GC'TnA_GC Cuts at: 3160 3160 Size: 5743 Bme1580I G_kGCm'C Cuts at: 1790 2539 2669 2745 2955 3804 4960 1790 Size: 749 130 76 210 849 1156 2573 Fragments arranged by size: 2573 1156 849 749 210 130 76 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5743 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3474 4432 442 Size: 416 53 83 399 24 2057 958 1753 Fragments arranged by size: 2057 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5743 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2774 3056 4403 1239 Size: 1535 282 1347 2579 Fragments arranged by size: 2579 1535 1347 282 Bpu10I CC'TnA_GC Cuts at: 2913 2913 Size: 5743 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2084 2324 2411 2664 3011 3868 4733 5031 2084 Size: 240 87 253 347 857 865 298 2796 Fragments arranged by size: 2796 865 857 347 298 253 240 87 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4406 882 Size: 617 2907 2219 Fragments arranged by size: 2907 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2074 2977 3534 493 Size: 711 870 903 557 2702 Fragments arranged by size: 2702 903 870 711 557 BsaBI GATnn'nnATC Cuts at: 5737 5737 Size: 5743 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2043 2556 2772 3550 Size: 53 83 186 936 510 513 216 778 Cuts at: 3550 3932 4567 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 778 635 513 510 382 216 186 83 53 BsaWI w'CCGG_w Cuts at: 4176 4574 4848 4995 5406 4176 Size: 398 274 147 411 4513 Fragments arranged by size: 4513 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2220 2250 2708 2738 2987 Size: 30 165 30 817 30 458 30 249 Cuts at: 2987 3017 1178 Size: 30 3904 Fragments arranged by size: 3904 817 458 249 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2771 2811 2843 3008 1186 Size: 1585 40 32 165 3921 Fragments arranged by size: 3921 1585 165 40 32 BseYI C'CCAG_C Cuts at: 2797 4946 5442 2797 Size: 2149 496 3098 Fragments arranged by size: 3098 2149 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5170 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3193 3954 4982 1540 Size: 275 186 1192 761 1028 2301 Fragments arranged by size: 2301 1192 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2526 2697 3804 3889 4960 2526 Size: 171 1107 85 1071 3309 Fragments arranged by size: 3309 1107 1071 171 85 BsmI GAATG_Cn' Cuts at: 3254 3347 3254 Size: 93 5650 BsmBI CGTCTCn'nnnn_ Cuts at: 2779 2779 Size: 5743 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2518 2526 2539 2669 2697 2745 2955 Size: 399 728 8 13 130 28 48 210 Cuts at: 2955 3062 3804 3889 4960 1391 Size: 107 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 728 399 210 130 107 85 48 28 13 8 BspEI T'CCGG_A Cuts at: 5406 5406 Size: 5743 BspHI T'CATG_A Cuts at: 3632 5362 3632 Size: 1730 4013 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2334 2401 2409 2728 2938 3098 844 Size: 1490 67 8 319 210 160 3489 Fragments arranged by size: 3489 1490 319 210 160 67 8 BsrBI CCG'CTC Cuts at: 1394 1823 3630 1394 Size: 429 1807 3507 Fragments arranged by size: 3507 1807 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4238 4412 65 Size: 1183 2990 174 1396 Fragments arranged by size: 2990 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2982 4387 4574 1700 Size: 110 1172 1405 187 2869 Fragments arranged by size: 2869 1405 1172 187 110 BsrGI T'GTAC_A Cuts at: 96 2374 96 Size: 2278 3465 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5743 BssSI C'ACGA_G Cuts at: 3803 4815 3803 Size: 1012 4731 BstAPI GCAn_nnn'nTGC Cuts at: 794 2335 794 Size: 1541 4202 BstBI TT'CG_AA Cuts at: 4547 4547 Size: 5743 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5743 BstXI CCAn_nnnn'nTGG Cuts at: 2074 2752 2074 Size: 678 5065 BstYI r'GATC_y Cuts at: 1972 2263 2721 2791 3073 3440 3825 3842 5294 Size: 291 458 70 282 367 385 17 1452 Cuts at: 5294 5380 5392 5738 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 458 385 367 346 291 282 86 70 17 12 Bsu36I CC'TnA_GG Cuts at: 2114 2416 2114 Size: 302 5441 BtgI C'CryG_G Cuts at: 513 1065 2004 2047 513 Size: 552 939 43 4209 Fragments arranged by size: 4209 939 552 43 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2818 487 Size: 683 56 1592 3412 Fragments arranged by size: 3412 1592 683 56 BtsI GCAGTG_nn' Cuts at: 3259 4053 4073 3259 Size: 794 20 4929 Fragments arranged by size: 4929 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5743 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5708 DraI TTT'AAA Cuts at: 3047 3047 Size: 5743 DraIII CAC_nnn'GTG Cuts at: 2225 2494 2505 2225 Size: 269 11 5463 Fragments arranged by size: 5463 269 11 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2555 4750 809 Size: 507 1239 2195 1802 Fragments arranged by size: 2195 1802 1239 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2268 2496 2687 Size: 54 1425 50 275 18 438 228 191 Cuts at: 2687 3190 4079 5708 8 Size: 503 889 1629 43 Fragments arranged by size: 1629 1425 889 503 438 275 228 191 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3190 1537 Size: 275 1378 4090 Fragments arranged by size: 4090 1378 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3673 1353 Size: 2320 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4330 4704 4850 387 Size: 1064 138 2741 374 146 1280 Fragments arranged by size: 2741 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2524 2695 2524 Size: 171 5572 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2225 2774 3056 3806 4403 5190 5458 Size: 393 593 549 282 750 597 787 268 Cuts at: 5458 1239 Size: 1524 Fragments arranged by size: 1524 787 750 597 593 549 393 282 268 EcoNI CCTnn'n_nnAGG Cuts at: 2102 2756 2102 Size: 654 5089 EcoO109I rG'GnC_Cy Cuts at: 2146 2383 2604 2927 2951 2952 2146 Size: 237 221 323 24 1 4937 Fragments arranged by size: 4937 323 237 221 24 1 EcoRI G'AATT_C Cuts at: 3052 3430 3052 Size: 378 5365 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2743 2743 Size: 5743 FspI TGC'GCA Cuts at: 4249 4249 Size: 5743 HaeII r_GCGC'y Cuts at: 1678 2046 2415 4890 1678 Size: 368 369 2475 2531 Fragments arranged by size: 2531 2475 369 368 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2149 2181 2552 2584 4384 Size: 32 135 32 577 32 371 32 1800 Cuts at: 4384 4416 4458 4490 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1800 577 371 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3087 3333 669 Size: 988 281 1149 246 3079 Fragments arranged by size: 3079 1149 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5743 HpaI GTT'AAC Cuts at: 3333 3333 Size: 5743 KasI G'GCGC_C Cuts at: 2042 2042 Size: 5743 KpnI G_GTAC'C Cuts at: 2512 3068 2512 Size: 556 5187 MfeI C'AATT_G Cuts at: 3342 3342 Size: 5743 MluI A'CGCG_T Cuts at: 5619 5619 Size: 5743 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2103 2241 4857 5041 1305 Size: 798 138 2616 184 2007 Fragments arranged by size: 2616 2007 798 184 138 MscI TGG'CCA Cuts at: 10 64 1489 1832 2498 10 Size: 54 1425 343 666 3255 Fragments arranged by size: 3255 1425 666 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2964 3701 4060 4219 518 Size: 648 1798 737 359 159 2042 Fragments arranged by size: 2042 1798 737 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3159 3836 4984 5229 1606 Size: 1553 677 1148 245 2120 Fragments arranged by size: 2120 1553 1148 677 245 NarI GG'CG_CC Cuts at: 2043 2043 Size: 5743 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4252 Fragments arranged by size: 4252 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5743 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5743 NotI GC'GGCC_GC Cuts at: 3190 3190 Size: 5743 NspI r_CATG'y Cuts at: 1132 1229 3101 4646 1132 Size: 97 1872 1545 2229 Fragments arranged by size: 2229 1872 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5743 PciI A'CATG_T Cuts at: 4642 4642 Size: 5743 PflMI CCAn_nnn'nTGG Cuts at: 1108 2225 2505 2788 1108 Size: 1117 280 283 4063 Fragments arranged by size: 4063 1117 283 280 PfoI T'CCnGG_A Cuts at: 3033 3033 Size: 5743 PmeI GTTT'AAAC Cuts at: 3047 3047 Size: 5743 PmlI CAC'GTG Cuts at: 2074 2977 2074 Size: 903 4840 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3812 3844 5325 5357 3812 Size: 32 1481 32 4198 Fragments arranged by size: 4198 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2383 2604 2927 2383 Size: 221 323 5199 Fragments arranged by size: 5199 323 221 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5743 PsiI TTA'TAA Cuts at: 3313 3313 Size: 5743 PspOMI G'GGCC_C Cuts at: 2951 2951 Size: 5743 PspXI vC'TCGA_Gb Cuts at: 2451 2451 Size: 5743 PstI C_TGCA'G Cuts at: 830 2172 2331 3095 830 Size: 1342 159 764 3478 Fragments arranged by size: 3478 1342 764 159 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5743 SacI G_AGCT'C Cuts at: 2526 2697 2526 Size: 171 5572 SalI G'TCGA_C Cuts at: 1936 3085 1936 Size: 1149 4594 SanDI GG'GwC_CC Cuts at: 2604 2604 Size: 5743 SbfI CC_TGCA'GG Cuts at: 3095 3095 Size: 5743 ScaI AGT'ACT Cuts at: 2015 2864 3991 5703 2015 Size: 849 1127 1712 2055 Fragments arranged by size: 2055 1712 1127 849 SexAI A'CCwGG_T Cuts at: 2490 2501 2490 Size: 11 5732 SfcI C'TryA_G Cuts at: 826 1045 2168 2327 3091 4226 4907 5098 826 Size: 219 1123 159 764 1135 681 191 1471 Fragments arranged by size: 1471 1135 1123 764 681 219 191 159 SfoI GGC'GCC Cuts at: 2044 2044 Size: 5743 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5743 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5743 SmaI CCC'GGG Cuts at: 1872 2619 3070 1872 Size: 747 451 4545 Fragments arranged by size: 4545 747 451 SmlI C'TyrA_G Cuts at: 820 1017 1943 2099 2339 2426 2451 2679 2990 Size: 197 926 156 240 87 25 228 311 Cuts at: 2990 3847 4748 5010 820 Size: 857 901 262 1553 Fragments arranged by size: 1553 926 901 857 311 262 240 228 197 156 87 25 SnaBI TAC'GTA Cuts at: 493 3534 493 Size: 3041 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5743 SphI G_CATG'C Cuts at: 1132 3101 1132 Size: 1969 3774 SspI AAT'ATT Cuts at: 5 52 3667 5 Size: 47 3615 2081 Fragments arranged by size: 3615 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5743 StyI C'CwwG_G Cuts at: 513 1065 2004 2844 2937 3182 513 Size: 552 939 840 93 245 3074 Fragments arranged by size: 3074 939 840 552 245 93 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3770 3787 3940 4125 4570 325 Size: 1255 2190 17 153 185 445 1498 Fragments arranged by size: 2190 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2013 2374 2862 Size: 274 80 33 51 1443 36 361 488 Cuts at: 2862 3989 5701 96 Size: 1127 1712 138 Fragments arranged by size: 1712 1443 1127 488 361 274 138 80 51 36 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2220 2252 5703 5735 2220 Size: 32 3451 32 2228 Fragments arranged by size: 3451 2228 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5743 XbaI T'CTAG_A Cuts at: 3079 3079 Size: 5743 XhoI C'TCGA_G Cuts at: 1943 2451 1943 Size: 508 5235 XmaI C'CCGG_G Cuts at: 1870 2617 3068 1870 Size: 747 451 4545 Fragments arranged by size: 4545 747 451 XmnI GAAnn'nnTTC Cuts at: 3872 3872 Size: 5743 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2773 3551 276 Size: 53 83 186 936 1239 778 2468 Fragments arranged by size: 2468 1239 936 778 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PsrI PvuII RsrII SacII SapI SfiI SrfI SwaI XcmI