Restriction map for
pFN21AE3413
(Linear) (Six-base) MAPSORT of: KIEE3413.seq Check: 9415 from: 1 to: 1038
KIEE3413.orf
With 182 enzymes: SgfI *
March 22, 2012 16:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 723 1038
Size: 723 315
AatII G_ACGT'C
Cuts at: 0 770 1038
Size: 770 268
Acc65I G'GTAC_C
Cuts at: 0 503 1038
Size: 503 535
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 1038
Size: 220 818
AlwNI CAG_nnn'CTG
Cuts at: 0 144 330 1038
Size: 144 186 708
Fragments arranged by size:
708 186 144
ApaI G_GGCC'C
Cuts at: 0 950 1038
Size: 950 88
AvaI C'yCGr_G
Cuts at: 0 446 514 612 1038
Size: 446 68 98 426
Fragments arranged by size:
446 426 98 68
BanI G'GyrC_C
Cuts at: 0 37 503 529 661 735 1038
Size: 37 466 26 132 74 303
Fragments arranged by size:
466 303 132 74 37 26
BanII G_rGCy'C
Cuts at: 0 513 521 692 950 1038
Size: 513 8 171 258 88
Fragments arranged by size:
513 258 171 88 8
BbeI G_GCGC'C
Cuts at: 0 41 1038
Size: 41 997
BbsI GAAGACnn'nnnn_
Cuts at: 0 45 1038
Size: 45 993
BbvCI CC'TCA_GC
Cuts at: 0 908 1038
Size: 908 130
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 70 104 1038
Size: 70 34 934
Fragments arranged by size:
934 70 34
BglI GCCn_nnn'nGGC
Cuts at: 0 934 1038
Size: 934 104
BglII A'GATC_T
Cuts at: 0 716 1038
Size: 716 322
Bme1580I G_kGCm'C
Cuts at: 0 534 664 740 950 1038
Size: 534 130 76 210 88
Fragments arranged by size:
534 210 130 88 76
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 769 1038
Size: 769 269
Bpu10I CC'TnA_GC
Cuts at: 0 908 1038
Size: 908 130
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 319 406 659 1006 1038
Size: 79 240 87 253 347 32
Fragments arranged by size:
347 253 240 87 79 32
BsaAI yAC'GTr
Cuts at: 0 69 972 1038
Size: 69 903 66
Fragments arranged by size:
903 69 66
BsaHI Gr'CG_yC
Cuts at: 0 38 551 767 1038
Size: 38 513 216 271
Fragments arranged by size:
513 271 216 38
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 215 245 703 733 982 1012 1038
Size: 215 30 458 30 249 30 26
Fragments arranged by size:
458 249 215 30 30 30 26
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 766 806 838 1003 1038
Size: 766 40 32 165 35
Fragments arranged by size:
766 165 40 35 32
BseYI C'CCAG_C
Cuts at: 0 792 1038
Size: 792 246
BsiHKAI G_wGCw'C
Cuts at: 0 521 692 1038
Size: 521 171 346
Fragments arranged by size:
521 346 171
BsmBI CGTCTCn'nnnn_
Cuts at: 0 774 1038
Size: 774 264
Bsp1286I G_dGCh'C
Cuts at: 0 513 521 534 664 692 740 950 1038
Size: 513 8 13 130 28 48 210 88
Fragments arranged by size:
513 210 130 88 48 28 13 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 329 396 404 723 933 1038
Size: 329 67 8 319 210 105
Fragments arranged by size:
329 319 210 105 67 8
BsrFI r'CCGG_y
Cuts at: 0 977 1038
Size: 977 61
BsrGI T'GTAC_A
Cuts at: 0 369 1038
Size: 369 669
BstAPI GCAn_nnn'nTGC
Cuts at: 0 330 1038
Size: 330 708
BstXI CCAn_nnnn'nTGG
Cuts at: 0 69 747 1038
Size: 69 678 291
Fragments arranged by size:
678 291 69
BstYI r'GATC_y
Cuts at: 0 258 716 786 1038
Size: 258 458 70 252
Fragments arranged by size:
458 258 252 70
Bsu36I CC'TnA_GG
Cuts at: 0 109 411 1038
Size: 109 302 627
Fragments arranged by size:
627 302 109
BtgI C'CryG_G
Cuts at: 0 42 1038
Size: 42 996
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 813 1038
Size: 813 225
DraIII CAC_nnn'GTG
Cuts at: 0 220 489 500 1038
Size: 220 269 11 538
Fragments arranged by size:
538 269 220 11
DrdI GACnn_nn'nnGTC
Cuts at: 0 550 1038
Size: 550 488
EaeI y'GGCC_r
Cuts at: 0 263 491 682 1038
Size: 263 228 191 356
Fragments arranged by size:
356 263 228 191
EcoICRI GAG'CTC
Cuts at: 0 519 690 1038
Size: 519 171 348
Fragments arranged by size:
519 348 171
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 769 1038
Size: 220 549 269
Fragments arranged by size:
549 269 220
EcoNI CCTnn'n_nnAGG
Cuts at: 0 97 751 1038
Size: 97 654 287
Fragments arranged by size:
654 287 97
EcoO109I rG'GnC_Cy
Cuts at: 0 141 378 599 922 946 947 1038
Size: 141 237 221 323 24 1 91
Fragments arranged by size:
323 237 221 141 91 24 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 738 1038
Size: 738 300
HaeII r_GCGC'y
Cuts at: 0 41 410 1038
Size: 41 369 628
Fragments arranged by size:
628 369 41
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 144 176 547 579 1038
Size: 144 32 371 32 459
Fragments arranged by size:
459 371 144 32 32
KasI G'GCGC_C
Cuts at: 0 37 1038
Size: 37 1001
KpnI G_GTAC'C
Cuts at: 0 507 1038
Size: 507 531
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 98 236 1038
Size: 98 138 802
Fragments arranged by size:
802 138 98
MscI TGG'CCA
Cuts at: 0 493 1038
Size: 493 545
MslI CAynn'nnrTG
Cuts at: 0 959 1038
Size: 959 79
NarI GG'CG_CC
Cuts at: 0 38 1038
Size: 38 1000
PflMI CCAn_nnn'nTGG
Cuts at: 0 220 500 783 1038
Size: 220 280 283 255
Fragments arranged by size:
283 280 255 220
PfoI T'CCnGG_A
Cuts at: 0 1028 1038
Size: 1028 10
PmlI CAC'GTG
Cuts at: 0 69 972 1038
Size: 69 903 66
Fragments arranged by size:
903 69 66
PpuMI rG'GwC_Cy
Cuts at: 0 378 599 922 1038
Size: 378 221 323 116
Fragments arranged by size:
378 323 221 116
PspOMI G'GGCC_C
Cuts at: 0 946 1038
Size: 946 92
PspXI vC'TCGA_Gb
Cuts at: 0 446 1038
Size: 446 592
PstI C_TGCA'G
Cuts at: 0 167 326 1038
Size: 167 159 712
Fragments arranged by size:
712 167 159
SacI G_AGCT'C
Cuts at: 0 521 692 1038
Size: 521 171 346
Fragments arranged by size:
521 346 171
SanDI GG'GwC_CC
Cuts at: 0 599 1038
Size: 599 439
ScaI AGT'ACT
Cuts at: 0 10 859 1038
Size: 10 849 179
Fragments arranged by size:
849 179 10
SexAI A'CCwGG_T
Cuts at: 0 485 496 1038
Size: 485 11 542
Fragments arranged by size:
542 485 11
SfcI C'TryA_G
Cuts at: 0 163 322 1038
Size: 163 159 716
Fragments arranged by size:
716 163 159
SfoI GGC'GCC
Cuts at: 0 39 1038
Size: 39 999
SmaI CCC'GGG
Cuts at: 0 614 1038
Size: 614 424
SmlI C'TyrA_G
Cuts at: 0 94 334 421 446 674 985 1038
Size: 94 240 87 25 228 311 53
Fragments arranged by size:
311 240 228 94 87 53 25
StyI C'CwwG_G
Cuts at: 0 839 932 1038
Size: 839 93 106
Fragments arranged by size:
839 106 93
TatI w'GTAC_w
Cuts at: 0 8 369 857 1038
Size: 8 361 488 181
Fragments arranged by size:
488 361 181 8
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 215 247 1038
Size: 215 32 791
Fragments arranged by size:
791 215 32
XhoI C'TCGA_G
Cuts at: 0 446 1038
Size: 446 592
XmaI C'CCGG_G
Cuts at: 0 612 1038
Size: 612 426
ZraI GAC'GTC
Cuts at: 0 768 1038
Size: 768 270
Enzymes that do cut:
AarI AatII Acc65I AcuI AlwNI ApaI AvaI BanI
BanII BbeI BbsI BbvCI BcgI BglI BglII Bme1580I
BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI BseYI
BsiHKAI BsmBI Bsp1286I BspMI BsrFI BsrGI BstAPI BstXI
BstYI Bsu36I BtgI BtgZI DraIII DrdI EaeI EcoICRI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KasI KpnI
MmeI MscI MslI NarI PflMI PfoI PmlI PpuMI
PspOMI PspXI PstI SacI SanDI ScaI SexAI SfcI
SfoI SmaI SmlI StyI TatI TstI XhoI XmaI
ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BciVI BclI BfrBI BlpI BmgBI BmrI
BmtI BplI BsaI BsaBI BsaWI BsgI BsiEI BsiWI
BsmI BspEI BspHI BsrBI BsrDI BssHII BssSI BstBI
BstEII BstZ17I BtsI ClaI CspCI DraI EagI EarI
EciI EcoRI EcoRV FseI FspI FspAI HincII HindIII
HpaI MfeI MluI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PmeI PpiI PshAI PsiI PsrI PvuI PvuII RsrII
SacII SalI SapI SbfI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII Tth111I
XbaI XcmI XmnI