Restriction map for
pFN21AE2313
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# NO CUT ENZYMES ( 149 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BlnI
BlpI BmyI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiHKAI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1286I BspDI BspEI BspHI BspMI
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HpaII KasI KpnI KspI MamI
MfeI MluI MroI MspI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SwaI Tth111I Van91I
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 1627 GTAGAC GT^[AC][GT]AC
AciI 1863 GCGG CCGC(-3/-1)
AflIII 912 ACATGT A^C[GA][CT]GT
Asp700I 679 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 721 GGTCC G^G[AT]CC
BalI 1493 TGGCCA TGG^CCA
BamHI 1698 GGATCC G^GATCC
BbsI 1153 GAAGAC GAAGAC(2/6)
BglII 1749 AGATCT A^GATCT
BpuAI 1153 GAAGAC GAAGAC(2/6)
BsaI 1184 GAGACC GGTCTC(1/5)
BseRI 949 GAGGAG GAGGAG(10/8)
BsiEI 1035 CGATCG CG[GA][CT]^CG
BsiYI 712 CCTGCATTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 712 CCTGCATTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1407I 1350 TGTACA T^GTACA
BspLU11I 912 ACATGT A^CATGT
BsrDI 1807 GCAATG GCAATG(2/0)
BsrGI 1350 TGTACA T^GTACA
BstXI 370 CCAAAACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 720 AGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 1448 CCATGG C^C[GA][CT]GG
EaeI 1493 TGGCCA [CT]^GGCC[GA]
EarI 1703 CTCTTC CTCTTC(1/4)
EcoNI 712 CCTGCATTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 720 AGGTCCT [GA]G^G[ACGT]CC[CT]
HindIII 83 AAGCTT A^AGCTT
HpaI 121 GTTAAC GTT^AAC
Ksp632I 1703 CTCTTC CTCTTC(1/4)
MaeII 557 ACGT A^CGT
MluNI 1493 TGGCCA TGG^CCA
MscI 1493 TGGCCA TGG^CCA
MslI 1213 CATTGTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 350 CAGCTG C[AC]G^C[GT]G
MwoI 19 GCATTAAGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 1448 CCATGG C^CATGG
PleI 129 GAGTC GAGTC(4/5)
PpuMI 720 AGGTCCT [GA]G^G[AT]CC[CT]
PvuI 1035 CGATCG CGAT^CG
PvuII 350 CAGCTG CAG^CTG
ScaI 615 AGTACT AGT^ACT
SpeI 10 ACTAGT A^CTAGT
SspBI 1350 TGTACA T^GTACA
SspI 1323 AATATT AAT^ATT
StuI 953 AGGCCT AGG^CCT
TaqI 1038 TCGA T^CGA
Tsp45I 7 GTGAC ^GT[GC]AC
TthHB8I 1038 TCGA T^CGA
XmnI 679 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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BpmI 485 CTCCAG CTGGAG(16/14)
946 CTGGAG
BsmAI 1184 GAGAC GTCTC(1/5)
1264 GAGAC
BssKI 487 CCAGG ^CC[ACGT]GG
1722 CCTGG
BstNI 487 CCAGG CC^[AT]GG
1722 CCTGG
Cac8I 291 GCCTGC GC[ACGT]^[ACGT]GC
1495 GCCAGC
Cfr13I 135 GGCCC G^G[ACGT]CC
721 GGTCC
EcoRII 487 CCAGG ^CC[AT]GG
1722 CCTGG
HincII 121 GTTAAC GT[CT]^[GA]AC
1342 GTCAAC
HindII 121 GTTAAC GT[CT]^[GA]AC
1342 GTCAAC
HphI 574 GGTGA GGTGA(8/7)
806 TCACC
MvaI 487 CCAGG CC^[AT]GG
1722 CCTGG
NspI 54 GCATGT [GA]CATG^[CT]
912 ACATGT
PstI 259 CTGCAG CTGCA^G
409 CTGCAG
Sau96I 135 GGCCC G^G[ACGT]CC
721 GGTCC
ScrFI 487 CCAGG CC^[ACGT]GG
1722 CCTGG
SfcI 259 CTGCAG C^T[GA][CT]AG
409 CTGCAG
XbaI 705 TCTAGA T^CTAGA
1620 TCTAGA
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# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AseI AsnI BbvI BfaI BsaJI
BsrI BstF5I BstYI DdeI DpnII
EcoT14I Fnu4HI FokI HaeIII HinfI
ItaI MaeI MaeIII MboI MboII
MflI MnlI MseI NdeII NlaIII
NlaIV PshBI RsaI Sau3AI SfaNI
SmlI StyI TfiI Tru9I TseI
Tsp509I TspRI XhoII