(Circular) (Six-base) MAPSORT of: pFN21AE2313.seq Check: 3552 from: 1 to: 6580 pFN21AE2313 6580 bp With 182 enzymes: SgfI * January 12, 2011 17:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4390 278 Size: 53 83 186 936 2854 2468 Fragments arranged by size: 2854 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3633 3923 1937 Size: 1696 290 4594 Fragments arranged by size: 4594 1696 290 Acc65I G'GTAC_C Cuts at: 3901 3901 Size: 6580 AclI AA'CG_TT Cuts at: 1563 4707 5080 1563 Size: 3144 373 3063 Fragments arranged by size: 3144 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2379 4643 6027 6295 1632 Size: 747 2264 1384 268 1917 Fragments arranged by size: 2264 1917 1384 747 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6383 AflIII A'CryG_T Cuts at: 1203 2917 5479 6456 1203 Size: 1714 2562 977 1327 Fragments arranged by size: 2562 1714 1327 977 AgeI A'CCGG_T Cuts at: 5411 5411 Size: 6580 AhdI GACnn_n'nnGTC Cuts at: 5309 5309 Size: 6580 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6580 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6580 AlwNI CAG_nnn'CTG Cuts at: 4323 5895 4323 Size: 1572 5008 ApaLI G'TGCA_C Cuts at: 4637 5793 4637 Size: 1156 5424 ApoI r'AATT_y Cuts at: 1493 2229 2502 2867 3375 3889 4115 4267 6376 Size: 736 273 365 508 514 226 152 2109 Cuts at: 6376 1493 Size: 1697 Fragments arranged by size: 2109 1697 736 514 508 365 273 226 152 AseI AT'TA_AT Cuts at: 160 1032 2271 2633 3438 5134 160 Size: 872 1239 362 805 1696 1606 Fragments arranged by size: 1696 1606 1239 872 805 362 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6580 AvaI C'yCGr_G Cuts at: 1870 1943 3905 1870 Size: 73 1962 4545 Fragments arranged by size: 4545 1962 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2303 2336 2303 Size: 33 6547 BamHI G'GATC_C Cuts at: 3703 3910 4277 3703 Size: 207 367 6006 Fragments arranged by size: 6006 367 207 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3901 5356 618 Size: 325 217 627 2114 1455 1842 Fragments arranged by size: 2114 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3899 1391 Size: 2508 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3165 928 Size: 971 1266 4343 Fragments arranged by size: 4343 1266 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4771 4805 1903 Size: 34 2834 34 3678 Fragments arranged by size: 3678 2834 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4472 5682 4472 Size: 1210 5370 BclI T'GATC_A Cuts at: 1548 1548 Size: 6580 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5191 136 Size: 107 122 71 631 605 3519 1525 Fragments arranged by size: 3519 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3754 6575 3754 Size: 2821 3759 BlpI GC'TnA_GC Cuts at: 3997 3997 Size: 6580 Bme1580I G_kGCm'C Cuts at: 1790 4641 5797 1790 Size: 2851 1156 2573 Fragments arranged by size: 2851 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6580 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4311 5269 442 Size: 416 53 83 399 24 2894 958 1753 Fragments arranged by size: 2894 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6580 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2475 2972 5240 1239 Size: 1236 497 2268 2579 Fragments arranged by size: 2579 2268 1236 497 Bpu10I CC'TnA_GC Cuts at: 3232 3232 Size: 6580 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2152 3553 3872 4705 5570 5868 2152 Size: 1401 319 833 865 298 2864 Fragments arranged by size: 2864 1401 865 833 319 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3183 5243 882 Size: 617 1684 2060 2219 Fragments arranged by size: 2219 2060 1684 617 BsaAI yAC'GTr Cuts at: 493 1204 4371 493 Size: 711 3167 2702 Fragments arranged by size: 3167 2702 711 BsaBI GATnn'nnATC Cuts at: 6574 6574 Size: 6580 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4387 4769 5404 275 Size: 53 83 186 936 2854 382 635 1451 Fragments arranged by size: 2854 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5013 5411 5685 5832 6243 5013 Size: 398 274 147 411 5350 Fragments arranged by size: 5350 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2568 2598 1178 Size: 30 165 30 1165 30 5160 Fragments arranged by size: 5160 1165 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2969 1186 Size: 1783 4797 BseYI C'CCAG_C Cuts at: 5783 6279 5783 Size: 496 6084 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6007 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3043 4030 4791 5819 1540 Size: 275 186 1042 987 761 1028 2301 Fragments arranged by size: 2301 1042 1028 987 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4641 4726 5797 4641 Size: 85 1071 5424 Fragments arranged by size: 5424 1071 85 BsmI GAATG_Cn' Cuts at: 4091 4184 4091 Size: 93 6487 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3899 4641 4726 5797 1391 Size: 399 2109 742 85 1071 2174 Fragments arranged by size: 2174 2109 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6243 6243 Size: 6580 BspHI T'CATG_A Cuts at: 4469 6199 4469 Size: 1730 4850 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3935 844 Size: 3091 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4467 1394 Size: 429 2644 3507 Fragments arranged by size: 3507 2644 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3819 5075 5249 65 Size: 1183 2571 1256 174 1396 Fragments arranged by size: 2571 1396 1256 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5224 5411 1700 Size: 110 3414 187 2869 Fragments arranged by size: 3414 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3355 96 Size: 3259 3321 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6580 BssSI C'ACGA_G Cuts at: 4640 5652 4640 Size: 1012 5568 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6580 BstBI TT'CG_AA Cuts at: 5384 5384 Size: 6580 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6580 BstXI CCAn_nnnn'nTGG Cuts at: 2382 2382 Size: 6580 BstYI r'GATC_y Cuts at: 1972 2713 3703 3754 3910 4277 4662 4679 6131 Size: 741 990 51 156 367 385 17 1452 Cuts at: 6131 6217 6229 6575 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 990 741 385 367 346 156 86 51 17 12 BtgI C'CryG_G Cuts at: 513 1065 3453 513 Size: 552 2388 3640 Fragments arranged by size: 3640 2388 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5841 Fragments arranged by size: 5841 683 56 BtsI GCAGTG_nn' Cuts at: 2174 4096 4890 4910 2174 Size: 1922 794 20 3844 Fragments arranged by size: 3844 1922 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6580 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6545 DraI TTT'AAA Cuts at: 3884 3884 Size: 6580 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5587 809 Size: 507 4271 1802 Fragments arranged by size: 4271 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3498 4027 4916 Size: 54 1425 50 275 18 1668 529 889 Cuts at: 4916 6545 8 Size: 1629 43 Fragments arranged by size: 1668 1629 1425 889 529 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4027 1537 Size: 275 2215 4090 Fragments arranged by size: 4090 2215 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3714 4510 1353 Size: 2361 796 3423 Fragments arranged by size: 3423 2361 796 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5167 5541 5687 387 Size: 1064 138 3578 374 146 1280 Fragments arranged by size: 3578 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2379 2475 2972 4643 5240 6027 6295 Size: 393 747 96 497 1671 597 787 268 Cuts at: 6295 1239 Size: 1524 Fragments arranged by size: 1671 1524 787 747 597 497 393 268 96 EcoNI CCTnn'n_nnAGG Cuts at: 2721 2721 Size: 6580 EcoO109I rG'GnC_Cy Cuts at: 2726 2726 Size: 6580 EcoRI G'AATT_C Cuts at: 3889 4267 3889 Size: 378 6202 FspI TGC'GCA Cuts at: 5086 5086 Size: 6580 HaeII r_GCGC'y Cuts at: 1678 5727 1678 Size: 4049 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2780 2812 2885 2917 3330 Size: 32 135 32 1208 32 73 32 413 Cuts at: 3330 3362 5221 5253 5295 5327 1373 Size: 32 1859 32 42 32 2626 Fragments arranged by size: 2626 1859 1208 413 135 73 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2128 3349 3924 4170 669 Size: 988 281 190 1221 575 246 3079 Fragments arranged by size: 3079 1221 988 575 281 246 190 HindIII A'AGCT_T Cuts at: 748 2088 748 Size: 1340 5240 HpaI GTT'AAC Cuts at: 2128 4170 2128 Size: 2042 4538 KpnI G_GTAC'C Cuts at: 3905 3905 Size: 6580 MfeI C'AATT_G Cuts at: 4179 4179 Size: 6580 MluI A'CGCG_T Cuts at: 6456 6456 Size: 6580 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5694 5878 1305 Size: 4389 184 2007 Fragments arranged by size: 4389 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3500 10 Size: 54 1425 343 1668 3090 Fragments arranged by size: 3090 1668 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3222 4538 4897 5056 518 Size: 648 843 1213 1316 359 159 2042 Fragments arranged by size: 2042 1316 1213 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2357 3996 4673 5821 6066 1606 Size: 751 1639 677 1148 245 2120 Fragments arranged by size: 2120 1639 1148 751 677 245 NcoI C'CATG_G Cuts at: 513 1065 3453 513 Size: 552 2388 3640 Fragments arranged by size: 3640 2388 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6580 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6580 NotI GC'GGCC_GC Cuts at: 4027 4027 Size: 6580 NspI r_CATG'y Cuts at: 1132 1229 2063 2921 3938 5483 1132 Size: 97 834 858 1017 1545 2229 Fragments arranged by size: 2229 1545 1017 858 834 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6580 PciI A'CATG_T Cuts at: 2917 5479 2917 Size: 2562 4018 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6580 PfoI T'CCnGG_A Cuts at: 3726 3726 Size: 6580 PmeI GTTT'AAAC Cuts at: 3884 3884 Size: 6580 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4649 4681 6162 6194 4649 Size: 32 1481 32 5035 Fragments arranged by size: 5035 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2726 2726 Size: 6580 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6580 PsiI TTA'TAA Cuts at: 4150 4150 Size: 6580 PstI C_TGCA'G Cuts at: 830 2268 2418 3932 830 Size: 1438 150 1514 3478 Fragments arranged by size: 3478 1514 1438 150 PvuI CG_AT'CG Cuts at: 2001 3043 2001 Size: 1042 5538 PvuII CAG'CTG Cuts at: 2357 2357 Size: 6580 SalI G'TCGA_C Cuts at: 1936 3922 1936 Size: 1986 4594 SbfI CC_TGCA'GG Cuts at: 3932 3932 Size: 6580 ScaI AGT'ACT Cuts at: 2622 4828 6540 2622 Size: 2206 1712 2662 Fragments arranged by size: 2662 2206 1712 SfcI C'TryA_G Cuts at: 826 1045 2264 2414 3928 5063 5744 5935 826 Size: 219 1219 150 1514 1135 681 191 1471 Fragments arranged by size: 1514 1471 1219 1135 681 219 191 150 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6580 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6580 SmaI CCC'GGG Cuts at: 1872 3907 1872 Size: 2035 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2131 3568 3851 4684 5585 5847 Size: 197 926 188 1437 283 833 901 262 Cuts at: 5847 820 Size: 1553 Fragments arranged by size: 1553 1437 926 901 833 283 262 197 188 SnaBI TAC'GTA Cuts at: 493 4371 493 Size: 3878 2702 SpeI A'CTAG_T Cuts at: 152 2015 152 Size: 1863 4717 SphI G_CATG'C Cuts at: 1132 3938 1132 Size: 2806 3774 SspI AAT'ATT Cuts at: 5 52 3330 4504 5 Size: 47 3278 1174 2081 Fragments arranged by size: 3278 2081 1174 47 StuI AGG'CCT Cuts at: 1517 2960 1517 Size: 1443 5137 StyI C'CwwG_G Cuts at: 513 1065 2277 2381 3453 4019 513 Size: 552 1212 104 1072 566 3074 Fragments arranged by size: 3074 1212 1072 566 552 104 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4607 4624 4777 4962 5407 325 Size: 1255 3027 17 153 185 445 1498 Fragments arranged by size: 3027 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2620 3355 4826 Size: 274 80 33 51 1443 643 735 1471 Cuts at: 4826 6538 96 Size: 1712 138 Fragments arranged by size: 1712 1471 1443 735 643 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6540 6572 6540 Size: 32 6548 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6580 XbaI T'CTAG_A Cuts at: 2710 3625 3916 2710 Size: 915 291 5374 Fragments arranged by size: 5374 915 291 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6580 XmaI C'CCGG_G Cuts at: 1870 3905 1870 Size: 2035 4545 XmnI GAAnn'nnTTC Cuts at: 2688 4709 2688 Size: 2021 4559 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4388 276 Size: 53 83 186 936 2854 2468 Fragments arranged by size: 2854 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI