################################## # NO CUT ENZYMES ( 93 enzymes) # ################################## Acc65I AccIII AclI AflII AgeI AhdI AocI Aor51HI ApaI AscI Asp700I Asp718I AspEI AspI AviII AvrII BbrPI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BsrGI BssHII Bst1107I BstAPI BstBI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI Eam1105I Eco47III Eco81I EcoNI EcoRI EcoRV EcoT22I FspI HpaI KpnI MamI MfeI MroI MunI NcoI NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacI SalI SbfI ScaI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AatII 707 GACGTC GACGT^C AccI 1320 GTCTAC GT^[AC][GT]AC Alw44I 108 GTGCAC G^TGCAC ApaLI 108 GTGCAC G^TGCAC AseI 483 ATTAAT AT^TAAT AsnI 483 ATTAAT AT^TAAT AspHI 108 GTGCAC G[AT]GC[AT]^C BamHI 258 GGATCC G^GATCC BanII 578 GAGCCC G[GA]GC[CT]^C BbsI 46 GTCTTC GAAGAC(2/6) BbvCI 682 GCTGAGG CCTCAGC(-5/-2) BcgI 2106 GCACCTTGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1785 GGATAC GTATCC(6/5) BclI 936 TGATCA T^GATCA BpmI 82 CTGGAG CTGGAG(16/14) BpuAI 46 GTCTTC GAAGAC(2/6) BsaWI 1037 ACCGGA [AT]^CCGG[AT] BsiHKAI 108 GTGCAC G[AT]GC[AT]^C BspHI 1673 TCATGA T^CATGA BspMI 738 GCAGGT ACCTGC(4/8) BsrFI 129 GCCGGC [GA]^CCGG[CT] BssSI 351 CTCGTG CACGAG(-5/-1) BstEII 741 GGTGACC G^GT[ACGT]ACC BstPI 741 GGTGACC G^GT[ACGT]ACC BstXI 1943 CCATTATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 129 GCCGGC [GA]^CCGG[CT] DsaI 225 CCGCGG C^C[GA][CT]GG EcoO65I 741 GGTGACC G^GT[ACGT]ACC EcoT14I 1506 CCAAGG C^C[AT][AT]GG FbaI 936 TGATCA T^GATCA FseI 128 GGCCGGCC GGCCGG^CC HincII 1282 GTTGAC GT[CT]^[GA]AC HindII 1282 GTTGAC GT[CT]^[GA]AC HindIII 1881 AAGCTT A^AGCTT KspI 225 CCGCGG CCGC^GG MluI 783 ACGCGT A^CGCGT NaeI 129 GCCGGC GCC^GGC NdeI 494 CATATG CA^TATG NgoMIV 129 GCCGGC G^CCGGC PaeR7I 2114 CTCGAG C^TCGAG PflMI 153 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 244 GACTGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 483 ATTAAT AT^TAAT PvuII 1790 CAGCTG CAG^CTG RcaI 1673 TCATGA T^CATGA SacII 225 CCGCGG CCGC^GG SexAI 1580 ACCTGGT A^CC[AT]GGT StyI 1506 CCAAGG C^C[AT][AT]GG Van91I 153 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1147 TCTAGA T^CTAGA XcmI 393 CCAGGCGCGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 2114 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AlwNI 154 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG 940 CAGAGTCTG BglII 732 AGATCT A^GATCT 1105 AGATCT BsgI 2104 CTGCAC GTGCAG(16/14) 1399 GTGCAG BspLU11I 501 ACATGT A^CATGT 1870 ACATGT BsrBI 69 CCGCTC CCGCTC(-3/-3) 1209 CCGCTC BsrDI 564 CATTGC GCAATG(2/0) 2213 GCAATG DraII 217 GGGTCCC [GA]G^G[ACGT]CC[CT] 249 GGGTCCT DraIII 348 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG 1393 CACGGGGTG EcoO109I 217 GGGTCCC [GA]G^G[ACGT]CC[CT] 249 GGGTCCT MaeII 708 ACGT A^CGT 2041 ACGT MslI 543 CACAGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 564 CATTGCGGTG NspI 501 ACATGT [GA]CATG^[CT] 1870 ACATGT PpuMI 217 GGGTCCC [GA]G^G[AT]CC[CT] 249 GGGTCCT SapI 433 GAAGAGC GCTCTTC(1/4) 1211 GCTCTTC TfiI 636 GATTC G^A[AT]TC 1805 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 98 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AluI AlwI ApoI AvaI AvaII BalI BanI BbeI BbvI BcnI BfaI BmyI BsaHI BsaJI BseRI BsiEI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII EaeI EagI EarI EclXI Eco52I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII