(Circular) (Six-base) MAPSORT of: pFN21AE3288.seq Check: 34 from: 1 to: 6925 pFN21AE3288 6925 bp With 182 enzymes: SgfI * March 28, 2012 12:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2734 2734 Size: 6925 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2716 4735 278 Size: 53 83 186 936 1180 2019 2468 Fragments arranged by size: 2468 2019 1180 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3326 4268 1937 Size: 1389 942 4594 Fragments arranged by size: 4594 1389 942 Acc65I G'GTAC_C Cuts at: 4246 4246 Size: 6925 AclI AA'CG_TT Cuts at: 1563 5052 5425 1563 Size: 3489 373 3063 Fragments arranged by size: 3489 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2748 2888 3414 3820 4988 6372 6640 1632 Size: 1116 140 526 406 1168 1384 268 1917 Fragments arranged by size: 1917 1384 1168 1116 526 406 268 140 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6728 AflIII A'CryG_T Cuts at: 1203 2506 2788 3875 5824 6801 1203 Size: 1303 282 1087 1949 977 1327 Fragments arranged by size: 1949 1327 1303 1087 977 282 AgeI A'CCGG_T Cuts at: 5756 5756 Size: 6925 AhdI GACnn_n'nnGTC Cuts at: 5654 5654 Size: 6925 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6925 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2138 1184 Size: 954 5971 AlwNI CAG_nnn'CTG Cuts at: 2164 2950 4668 6240 2164 Size: 786 1718 1572 2849 Fragments arranged by size: 2849 1718 1572 786 ApaLI G'TGCA_C Cuts at: 2113 4982 6138 2113 Size: 2869 1156 2900 Fragments arranged by size: 2900 2869 1156 ApoI r'AATT_y Cuts at: 1493 2560 2829 3167 3698 4234 4460 4612 6721 Size: 1067 269 338 531 536 226 152 2109 Cuts at: 6721 1493 Size: 1697 Fragments arranged by size: 2109 1697 1067 536 531 338 269 226 152 AseI AT'TA_AT Cuts at: 160 1032 2489 5479 160 Size: 872 1457 2990 1606 Fragments arranged by size: 2990 1606 1457 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6925 AvaI C'yCGr_G Cuts at: 1870 1943 2077 2219 4119 4250 1870 Size: 73 134 142 1900 131 4545 Fragments arranged by size: 4545 1900 142 134 131 73 BamHI G'GATC_C Cuts at: 2263 4255 4622 2263 Size: 1992 367 4566 Fragments arranged by size: 4566 1992 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2082 2183 2241 4246 5701 Size: 325 217 627 295 101 58 2005 1455 Cuts at: 5701 618 Size: 1842 Fragments arranged by size: 2005 1842 1455 627 325 295 217 101 58 BanII G_rGCy'C Cuts at: 1391 2587 4244 1391 Size: 1196 1657 4072 Fragments arranged by size: 4072 1657 1196 BbeI G_GCGC'C Cuts at: 2086 2187 2245 2086 Size: 101 58 6766 Fragments arranged by size: 6766 101 58 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2044 928 Size: 971 145 5809 Fragments arranged by size: 5809 971 145 BbvCI CC'TCA_GC Cuts at: 2688 2688 Size: 6925 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4100 4134 5116 5150 1903 Size: 34 2163 34 982 34 3678 Fragments arranged by size: 3678 2163 982 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3784 4817 6027 3784 Size: 1033 1210 4682 Fragments arranged by size: 4682 1210 1033 BclI T'GATC_A Cuts at: 1548 2941 1548 Size: 1393 5532 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5536 136 Size: 107 122 71 631 605 337 3527 1525 Fragments arranged by size: 3527 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2737 3110 6920 2737 Size: 373 3810 2742 Fragments arranged by size: 3810 2742 373 BlpI GC'TnA_GC Cuts at: 4342 4342 Size: 6925 Bme1580I G_kGCm'C Cuts at: 1790 2110 2117 3082 4023 4986 6142 1790 Size: 320 7 965 941 963 1156 2573 Fragments arranged by size: 2573 1156 965 963 941 320 7 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6925 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2260 4656 5614 Size: 416 53 83 399 24 843 2396 958 Cuts at: 5614 442 Size: 1753 Fragments arranged by size: 2396 1753 958 843 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6925 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3370 3370 Size: 6925 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2108 5585 1239 Size: 869 3477 2579 Fragments arranged by size: 3477 2579 869 Bpu10I CC'TnA_GC Cuts at: 2688 2688 Size: 6925 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3029 3352 5050 5915 6213 3029 Size: 323 1698 865 298 3741 Fragments arranged by size: 3741 1698 865 323 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5588 882 Size: 617 4089 2219 Fragments arranged by size: 4089 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4716 493 Size: 711 3512 2702 Fragments arranged by size: 3512 2702 711 BsaBI GATnn'nnATC Cuts at: 6919 6919 Size: 6925 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2083 2142 2169 2184 Size: 53 83 186 936 550 59 27 15 Cuts at: 2184 2242 2713 4732 5114 5749 275 Size: 58 471 2019 382 635 1451 Fragments arranged by size: 2019 1451 936 635 550 471 382 186 83 59 58 53 27 15 BsaWI w'CCGG_w Cuts at: 3042 5358 5756 6030 6177 6588 3042 Size: 2316 398 274 147 411 3379 Fragments arranged by size: 3379 2316 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3227 3257 1178 Size: 30 165 30 1824 30 4846 Fragments arranged by size: 4846 1824 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2042 2111 2327 1186 Size: 856 69 216 5784 Fragments arranged by size: 5784 856 216 69 BseYI C'CCAG_C Cuts at: 3644 6128 6624 3644 Size: 2484 496 3945 Fragments arranged by size: 3945 2484 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3425 4094 1163 Size: 573 1689 669 3994 Fragments arranged by size: 3994 1689 669 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2135 2139 2176 2380 4375 5136 Size: 275 186 134 4 37 204 1995 761 Cuts at: 5136 6164 1540 Size: 1028 2301 Fragments arranged by size: 2301 1995 1028 761 275 204 186 134 37 4 BsiHKAI G_wGCw'C Cuts at: 2117 4986 5071 6142 2117 Size: 2869 85 1071 2900 Fragments arranged by size: 2900 2869 1071 85 BsmI GAATG_Cn' Cuts at: 4436 4529 4436 Size: 93 6832 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2110 2117 2587 3082 4023 4244 4986 Size: 399 320 7 470 495 941 221 742 Cuts at: 4986 5071 6142 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 941 742 495 470 399 320 221 85 7 BspEI T'CCGG_A Cuts at: 6588 6588 Size: 6925 BspHI T'CATG_A Cuts at: 3678 4814 6544 3678 Size: 1136 1730 4059 Fragments arranged by size: 4059 1730 1136 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2734 4280 844 Size: 1890 1546 3489 Fragments arranged by size: 3489 1890 1546 BsrBI CCG'CTC Cuts at: 1394 1823 2076 3216 4812 1394 Size: 429 253 1140 1596 3507 Fragments arranged by size: 3507 1596 1140 429 253 BsrDI GCAATG_nn' Cuts at: 65 1248 2568 4225 5420 5594 65 Size: 1183 1320 1657 1195 174 1396 Fragments arranged by size: 1657 1396 1320 1195 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2134 5569 5756 1700 Size: 110 324 3435 187 2869 Fragments arranged by size: 3435 2869 324 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6925 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6925 BssSI C'ACGA_G Cuts at: 2356 4985 5997 2356 Size: 2629 1012 3284 Fragments arranged by size: 3284 2629 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6925 BstBI TT'CG_AA Cuts at: 5729 5729 Size: 6925 BstEII G'GTnAC_C Cuts at: 1184 2746 1184 Size: 1562 5363 BstXI CCAn_nnnn'nTGG Cuts at: 3955 3955 Size: 6925 BstYI r'GATC_y Cuts at: 1972 2263 2737 2965 3110 3337 4157 4255 4622 Size: 291 474 228 145 227 820 98 367 Cuts at: 4622 5007 5024 6476 6562 6574 6920 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 820 474 385 367 346 291 228 227 145 98 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2230 513 Size: 552 939 226 5208 Fragments arranged by size: 5208 939 552 226 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6186 Fragments arranged by size: 6186 683 56 BtsI GCAGTG_nn' Cuts at: 3663 3984 4441 5235 5255 3663 Size: 321 457 794 20 5333 Fragments arranged by size: 5333 794 457 321 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6925 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6890 DraI TTT'AAA Cuts at: 4229 4229 Size: 6925 DraIII CAC_nnn'GTG Cuts at: 2358 3403 2358 Size: 1045 5880 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5932 809 Size: 507 4616 1802 Fragments arranged by size: 4616 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2132 2136 2349 Size: 54 1425 50 275 18 302 4 213 Cuts at: 2349 2377 2565 4038 4372 5261 6890 8 Size: 28 188 1473 334 889 1629 43 Fragments arranged by size: 1629 1473 1425 889 334 302 275 213 188 54 50 43 28 18 4 EagI C'GGCC_G Cuts at: 1537 1812 2132 2136 2377 4372 1537 Size: 275 320 4 241 1995 4090 Fragments arranged by size: 4090 1995 320 275 241 4 EarI CTCTTCn'nnn_ Cuts at: 1353 2433 2573 2616 3223 3456 4855 1353 Size: 1080 140 43 607 233 1399 3423 Fragments arranged by size: 3423 1399 1080 607 233 140 43 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2027 5512 5886 6032 387 Size: 1064 138 438 3485 374 146 1280 Fragments arranged by size: 3485 1280 1064 438 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2108 2748 2888 3414 3820 4988 5585 Size: 393 476 640 140 526 406 1168 597 Cuts at: 5585 6372 6640 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1168 787 640 597 526 476 406 393 268 140 EcoO109I rG'GnC_Cy Cuts at: 2223 2255 2223 Size: 32 6893 EcoRI G'AATT_C Cuts at: 4234 4612 4234 Size: 378 6547 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3850 3850 Size: 6925 FseI GG_CCGG'CC Cuts at: 2138 2138 Size: 6925 FspI TGC'GCA Cuts at: 5431 5431 Size: 6925 HaeII r_GCGC'y Cuts at: 1678 2086 2187 2245 2348 3720 6072 1678 Size: 408 101 58 103 1372 2352 2531 Fragments arranged by size: 2531 2352 1372 408 103 101 58 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3194 3226 3227 3259 4142 Size: 32 135 32 1622 32 1 32 883 Cuts at: 4142 4174 5566 5598 5640 5672 1373 Size: 32 1392 32 42 32 2626 Fragments arranged by size: 2626 1622 1392 883 135 42 32 32 32 32 32 32 32 1 HincII GTy'rAC Cuts at: 669 1657 1938 3289 4269 4515 669 Size: 988 281 1351 980 246 3079 Fragments arranged by size: 3079 1351 988 980 281 246 HindIII A'AGCT_T Cuts at: 748 3886 748 Size: 3138 3787 HpaI GTT'AAC Cuts at: 4515 4515 Size: 6925 KasI G'GCGC_C Cuts at: 2082 2183 2241 2082 Size: 101 58 6766 Fragments arranged by size: 6766 101 58 KpnI G_GTAC'C Cuts at: 4250 4250 Size: 6925 MfeI C'AATT_G Cuts at: 4524 4524 Size: 6925 MluI A'CGCG_T Cuts at: 2788 6801 2788 Size: 4013 2912 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2598 6039 6223 1305 Size: 1293 3441 184 2007 Fragments arranged by size: 3441 2007 1293 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2351 2567 4040 10 Size: 54 1425 343 519 216 1473 2895 Fragments arranged by size: 2895 1473 1425 519 343 216 54 MslI CAynn'nnrTG Cuts at: 518 1166 2552 2573 4883 5242 5401 518 Size: 648 1386 21 2310 359 159 2042 Fragments arranged by size: 2310 2042 1386 648 359 159 21 MspA1I CmG'CkG Cuts at: 1606 2161 2232 3318 3797 4341 5018 6166 6411 Size: 555 71 1086 479 544 677 1148 245 Cuts at: 6411 1606 Size: 2120 Fragments arranged by size: 2120 1148 1086 677 555 544 479 245 71 NaeI GCC'GGC Cuts at: 2136 2136 Size: 6925 NarI GG'CG_CC Cuts at: 2083 2184 2242 2083 Size: 101 58 6766 Fragments arranged by size: 6766 101 58 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5434 Fragments arranged by size: 5434 939 552 NdeI CA'TA_TG Cuts at: 387 2500 387 Size: 2113 4812 NgoMIV G'CCGG_C Cuts at: 2134 2134 Size: 6925 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6925 NotI GC'GGCC_GC Cuts at: 4372 4372 Size: 6925 NspI r_CATG'y Cuts at: 1132 1229 2510 3879 4283 5828 1132 Size: 97 1281 1369 404 1545 2229 Fragments arranged by size: 2229 1545 1369 1281 404 97 PasI CC'CwG_GG Cuts at: 1348 3724 4016 1348 Size: 2376 292 4257 Fragments arranged by size: 4257 2376 292 PciI A'CATG_T Cuts at: 2506 3875 5824 2506 Size: 1369 1949 3607 Fragments arranged by size: 3607 1949 1369 PflMI CCAn_nnn'nTGG Cuts at: 1108 2164 1108 Size: 1056 5869 PfoI T'CCnGG_A Cuts at: 2196 2196 Size: 6925 PmeI GTTT'AAAC Cuts at: 4229 4229 Size: 6925 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4994 5026 6507 6539 4994 Size: 32 1481 32 5380 Fragments arranged by size: 5380 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2223 2255 2223 Size: 32 6893 PshAI GACnn'nnGTC Cuts at: 1613 2253 1613 Size: 640 6285 PsiI TTA'TAA Cuts at: 2513 4495 2513 Size: 1982 4943 PstI C_TGCA'G Cuts at: 830 2653 2745 3270 3658 3783 4277 830 Size: 1823 92 525 388 125 494 3478 Fragments arranged by size: 3478 1823 525 494 388 125 92 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6925 PvuII CAG'CTG Cuts at: 3797 3797 Size: 6925 SacII CC_GC'GG Cuts at: 2233 2233 Size: 6925 SalI G'TCGA_C Cuts at: 1936 4267 1936 Size: 2331 4594 SanDI GG'GwC_CC Cuts at: 2223 2223 Size: 6925 SapI GCTCTTCn'nnn_ Cuts at: 2433 3223 2433 Size: 790 6135 SbfI CC_TGCA'GG Cuts at: 4277 4277 Size: 6925 ScaI AGT'ACT Cuts at: 5173 6885 5173 Size: 1712 5213 SexAI A'CCwGG_T Cuts at: 3585 3585 Size: 6925 SfcI C'TryA_G Cuts at: 826 1045 2649 2741 3266 3654 3779 3993 4273 Size: 219 1604 92 525 388 125 214 280 Cuts at: 4273 5408 6089 6280 826 Size: 1135 681 191 1471 Fragments arranged by size: 1604 1471 1135 681 525 388 280 219 214 191 125 92 SfoI GGC'GCC Cuts at: 2084 2185 2243 2084 Size: 101 58 6766 Fragments arranged by size: 6766 101 58 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6925 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6925 SmaI CCC'GGG Cuts at: 1872 4252 1872 Size: 2380 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3008 3331 4119 5029 5930 6192 Size: 197 926 1065 323 788 910 901 262 Cuts at: 6192 820 Size: 1553 Fragments arranged by size: 1553 1065 926 910 901 788 323 262 197 SnaBI TAC'GTA Cuts at: 493 4716 493 Size: 4223 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6925 SphI G_CATG'C Cuts at: 1132 4283 1132 Size: 3151 3774 SspI AAT'ATT Cuts at: 5 52 4849 5 Size: 47 4797 2081 Fragments arranged by size: 4797 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6925 StyI C'CwwG_G Cuts at: 513 1065 2004 3511 4364 513 Size: 552 939 1507 853 3074 Fragments arranged by size: 3074 1507 939 853 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4952 4969 5122 5307 5752 325 Size: 1255 3372 17 153 185 445 1498 Fragments arranged by size: 3372 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3637 5171 6883 Size: 274 80 33 51 1443 1660 1534 1712 Cuts at: 6883 96 Size: 138 Fragments arranged by size: 1712 1660 1534 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3265 3297 3427 3459 6885 6917 3265 Size: 32 130 32 3426 32 3273 Fragments arranged by size: 3426 3273 130 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6925 XbaI T'CTAG_A Cuts at: 3152 4261 3152 Size: 1109 5816 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2405 2405 Size: 6925 XhoI C'TCGA_G Cuts at: 1943 4119 1943 Size: 2176 4749 XmaI C'CCGG_G Cuts at: 1870 4250 1870 Size: 2380 4545 XmnI GAAnn'nnTTC Cuts at: 5054 5054 Size: 6925 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2714 4733 276 Size: 53 83 186 936 1180 2019 2468 Fragments arranged by size: 2468 2019 1180 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SfiI SrfI SwaI