(Linear) (Six-base) MAPSORT of: KIEE3288.seq Check: 8185 from: 1 to: 2220 KIEE3288.orf With 182 enzymes: SgfI * March 22, 2012 16:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 729 2220 Size: 729 1491 AatII G_ACGT'C Cuts at: 0 711 2220 Size: 711 1509 AccI GT'mk_AC Cuts at: 0 1321 2220 Size: 1321 899 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 743 883 1409 1815 2220 Size: 743 140 526 406 405 Fragments arranged by size: 743 526 406 405 140 AflIII A'CryG_T Cuts at: 0 501 783 1870 2220 Size: 501 282 1087 350 Fragments arranged by size: 1087 501 350 282 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 133 2220 Size: 133 2087 AlwNI CAG_nnn'CTG Cuts at: 0 159 945 2220 Size: 159 786 1275 Fragments arranged by size: 1275 786 159 ApaLI G'TGCA_C Cuts at: 0 108 2220 Size: 108 2112 ApoI r'AATT_y Cuts at: 0 555 824 1162 1693 2220 Size: 555 269 338 531 527 Fragments arranged by size: 555 531 527 338 269 AseI AT'TA_AT Cuts at: 0 484 2220 Size: 484 1736 AvaI C'yCGr_G Cuts at: 0 72 214 2114 2220 Size: 72 142 1900 106 Fragments arranged by size: 1900 142 106 72 BamHI G'GATC_C Cuts at: 0 258 2220 Size: 258 1962 BanI G'GyrC_C Cuts at: 0 77 178 236 2220 Size: 77 101 58 1984 Fragments arranged by size: 1984 101 77 58 BanII G_rGCy'C Cuts at: 0 582 2220 Size: 582 1638 BbeI G_GCGC'C Cuts at: 0 81 182 240 2220 Size: 81 101 58 1980 Fragments arranged by size: 1980 101 81 58 BbsI GAAGACnn'nnnn_ Cuts at: 0 39 2220 Size: 39 2181 BbvCI CC'TCA_GC Cuts at: 0 683 2220 Size: 683 1537 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2095 2129 2220 Size: 2095 34 91 Fragments arranged by size: 2095 91 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1779 2220 Size: 1779 441 BclI T'GATC_A Cuts at: 0 936 2220 Size: 936 1284 BglII A'GATC_T Cuts at: 0 732 1105 2220 Size: 732 373 1115 Fragments arranged by size: 1115 732 373 Bme1580I G_kGCm'C Cuts at: 0 105 112 1077 2018 2220 Size: 105 7 965 941 202 Fragments arranged by size: 965 941 202 105 7 BmrI ACTGGGnnnn_n' Cuts at: 0 255 2220 Size: 255 1965 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1365 2220 Size: 1365 855 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 103 2220 Size: 103 2117 Bpu10I CC'TnA_GC Cuts at: 0 683 2220 Size: 683 1537 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1024 1347 2220 Size: 1024 323 873 Fragments arranged by size: 1024 873 323 BsaHI Gr'CG_yC Cuts at: 0 78 137 164 179 237 708 2220 Size: 78 59 27 15 58 471 1512 Fragments arranged by size: 1512 471 78 59 58 27 15 BsaWI w'CCGG_w Cuts at: 0 1037 2220 Size: 1037 1183 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1222 1252 2220 Size: 1222 30 968 Fragments arranged by size: 1222 968 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 37 106 322 2220 Size: 37 69 216 1898 Fragments arranged by size: 1898 216 69 37 BseYI C'CCAG_C Cuts at: 0 1639 2220 Size: 1639 581 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1420 2089 2220 Size: 1420 669 131 Fragments arranged by size: 1420 669 131 BsiEI CG_ry'CG Cuts at: 0 130 134 171 375 2220 Size: 130 4 37 204 1845 Fragments arranged by size: 1845 204 130 37 4 BsiHKAI G_wGCw'C Cuts at: 0 112 2220 Size: 112 2108 Bsp1286I G_dGCh'C Cuts at: 0 105 112 582 1077 2018 2220 Size: 105 7 470 495 941 202 Fragments arranged by size: 941 495 470 202 105 7 BspHI T'CATG_A Cuts at: 0 1673 2220 Size: 1673 547 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 729 2220 Size: 729 1491 BsrBI CCG'CTC Cuts at: 0 71 1211 2220 Size: 71 1140 1009 Fragments arranged by size: 1140 1009 71 BsrDI GCAATG_nn' Cuts at: 0 563 2220 Size: 563 1657 BsrFI r'CCGG_y Cuts at: 0 129 2220 Size: 129 2091 BssSI C'ACGA_G Cuts at: 0 351 2220 Size: 351 1869 BstEII G'GTnAC_C Cuts at: 0 741 2220 Size: 741 1479 BstXI CCAn_nnnn'nTGG Cuts at: 0 1950 2220 Size: 1950 270 BstYI r'GATC_y Cuts at: 0 258 732 960 1105 1332 2152 2220 Size: 258 474 228 145 227 820 68 Fragments arranged by size: 820 474 258 228 227 145 68 BtgI C'CryG_G Cuts at: 0 225 2220 Size: 225 1995 BtsI GCAGTG_nn' Cuts at: 0 1658 1979 2220 Size: 1658 321 241 Fragments arranged by size: 1658 321 241 DraIII CAC_nnn'GTG Cuts at: 0 353 1398 2220 Size: 353 1045 822 Fragments arranged by size: 1045 822 353 EaeI y'GGCC_r Cuts at: 0 127 131 344 372 560 2033 2220 Size: 127 4 213 28 188 1473 187 Fragments arranged by size: 1473 213 188 187 127 28 4 EagI C'GGCC_G Cuts at: 0 127 131 372 2220 Size: 127 4 241 1848 Fragments arranged by size: 1848 241 127 4 EarI CTCTTCn'nnn_ Cuts at: 0 428 568 611 1218 1451 2220 Size: 428 140 43 607 233 769 Fragments arranged by size: 769 607 428 233 140 43 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 22 2220 Size: 22 2198 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 103 743 883 1409 1815 2220 Size: 103 640 140 526 406 405 Fragments arranged by size: 640 526 406 405 140 103 EcoO109I rG'GnC_Cy Cuts at: 0 218 250 2220 Size: 218 32 1970 Fragments arranged by size: 1970 218 32 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1845 2220 Size: 1845 375 FseI GG_CCGG'CC Cuts at: 0 133 2220 Size: 133 2087 HaeII r_GCGC'y Cuts at: 0 81 182 240 343 1715 2220 Size: 81 101 58 103 1372 505 Fragments arranged by size: 1372 505 103 101 81 58 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1189 1221 1222 1254 2137 2169 2220 Size: 1189 32 1 32 883 32 51 Fragments arranged by size: 1189 883 51 32 32 32 1 HincII GTy'rAC Cuts at: 0 1284 2220 Size: 1284 936 HindIII A'AGCT_T Cuts at: 0 1881 2220 Size: 1881 339 KasI G'GCGC_C Cuts at: 0 77 178 236 2220 Size: 77 101 58 1984 Fragments arranged by size: 1984 101 77 58 MluI A'CGCG_T Cuts at: 0 783 2220 Size: 783 1437 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 593 2220 Size: 593 1627 MscI TGG'CCA Cuts at: 0 346 562 2035 2220 Size: 346 216 1473 185 Fragments arranged by size: 1473 346 216 185 MslI CAynn'nnrTG Cuts at: 0 547 568 2220 Size: 547 21 1652 Fragments arranged by size: 1652 547 21 MspA1I CmG'CkG Cuts at: 0 156 227 1313 1792 2220 Size: 156 71 1086 479 428 Fragments arranged by size: 1086 479 428 156 71 NaeI GCC'GGC Cuts at: 0 131 2220 Size: 131 2089 NarI GG'CG_CC Cuts at: 0 78 179 237 2220 Size: 78 101 58 1983 Fragments arranged by size: 1983 101 78 58 NdeI CA'TA_TG Cuts at: 0 495 2220 Size: 495 1725 NgoMIV G'CCGG_C Cuts at: 0 129 2220 Size: 129 2091 NspI r_CATG'y Cuts at: 0 505 1874 2220 Size: 505 1369 346 Fragments arranged by size: 1369 505 346 PasI CC'CwG_GG Cuts at: 0 1719 2011 2220 Size: 1719 292 209 Fragments arranged by size: 1719 292 209 PciI A'CATG_T Cuts at: 0 501 1870 2220 Size: 501 1369 350 Fragments arranged by size: 1369 501 350 PflMI CCAn_nnn'nTGG Cuts at: 0 159 2220 Size: 159 2061 PfoI T'CCnGG_A Cuts at: 0 191 2220 Size: 191 2029 PpuMI rG'GwC_Cy Cuts at: 0 218 250 2220 Size: 218 32 1970 Fragments arranged by size: 1970 218 32 PshAI GACnn'nnGTC Cuts at: 0 248 2220 Size: 248 1972 PsiI TTA'TAA Cuts at: 0 508 2220 Size: 508 1712 PstI C_TGCA'G Cuts at: 0 648 740 1265 1653 1778 2220 Size: 648 92 525 388 125 442 Fragments arranged by size: 648 525 442 388 125 92 PvuII CAG'CTG Cuts at: 0 1792 2220 Size: 1792 428 SacII CC_GC'GG Cuts at: 0 228 2220 Size: 228 1992 SanDI GG'GwC_CC Cuts at: 0 218 2220 Size: 218 2002 SapI GCTCTTCn'nnn_ Cuts at: 0 428 1218 2220 Size: 428 790 1002 Fragments arranged by size: 1002 790 428 SexAI A'CCwGG_T Cuts at: 0 1580 2220 Size: 1580 640 SfcI C'TryA_G Cuts at: 0 644 736 1261 1649 1774 1988 2220 Size: 644 92 525 388 125 214 232 Fragments arranged by size: 644 525 388 232 214 125 92 SfoI GGC'GCC Cuts at: 0 79 180 238 2220 Size: 79 101 58 1982 Fragments arranged by size: 1982 101 79 58 SmlI C'TyrA_G Cuts at: 0 1003 1326 2114 2220 Size: 1003 323 788 106 Fragments arranged by size: 1003 788 323 106 StyI C'CwwG_G Cuts at: 0 1506 2220 Size: 1506 714 TatI w'GTAC_w Cuts at: 0 1632 2220 Size: 1632 588 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1260 1292 1422 1454 2220 Size: 1260 32 130 32 766 Fragments arranged by size: 1260 766 130 32 32 XbaI T'CTAG_A Cuts at: 0 1147 2220 Size: 1147 1073 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 400 2220 Size: 400 1820 XhoI C'TCGA_G Cuts at: 0 2114 2220 Size: 2114 106 ZraI GAC'GTC Cuts at: 0 709 2220 Size: 709 1511 Enzymes that do cut: AarI AatII AccI AcuI AflIII AlfI AlwNI ApaLI ApoI AseI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstEII BstXI BstYI BtgI BtsI DraIII EaeI EagI EarI EciI Eco57MI EcoO109I FalI FseI HaeII Hin4I HincII HindIII KasI MluI MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV NspI PasI PciI PflMI PfoI PpuMI PshAI PsiI PstI PvuII SacII SanDI SapI SexAI SfcI SfoI SmlI StyI TatI TstI XbaI XcmI XhoI ZraI Enzymes that do not cut: Acc65I AclI AfeI AflII AgeI AhdI AleI AloI ApaI AscI AsiSI AvrII BaeI BfrBI BglI BlpI BmgBI BmtI BsaI BsaAI BsaBI BsiWI BsmI BsmBI BspEI BsrGI BssHII BstAPI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EcoICRI EcoNI EcoRI EcoRV FspI FspAI HpaI KpnI MfeI NcoI NheI NotI NruI NsiI PacI PmeI PmlI PpiI PspOMI PspXI PsrI PvuI RsrII SacI SalI SbfI ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII Tth111I XmaI XmnI