################################## # NO CUT ENZYMES ( 189 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvI BcgI BclI BfrI BglI BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII Cfr10I ClaI CpoI DraI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI Fnu4HI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI HphI ItaI KasI KpnI KspI MaeII MamI MfeI MluI MroI MslI MspA1I MunI MvnI MwoI NaeI NarI NcoI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TseI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AluI 343 AGCT AG^CT AlwNI 122 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 292 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 525 GGACC G^G[AT]CC BalI 432 TGGCCA TGG^CCA BbvCI 265 GCTGAGG CCTCAGC(-5/-2) BciVI 421 GGATAC GTATCC(6/5) BcnI 418 CCGGG CC^[GC]GG BfaI 565 CTAG C^TAG BglII 549 AGATCT A^GATCT BpmI 407 CTCCAG CTGGAG(16/14) BsmAI 136 GAGAC GTCTC(1/5) BsrI 54 ACTGG ACTGG(1/-1) BstYI 549 AGATCT [GA]^GATC[CT] CfoI 227 GCGC GCG^C DpnII 550 GATC ^GATC DraII 524 AGGACCT [GA]G^G[ACGT]CC[CT] DraIII 235 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 432 TGGCCA [CT]^GGCC[GA] EarI 520 GAAGAG CTCTTC(1/4) EcoO109I 524 AGGACCT [GA]G^G[ACGT]CC[CT] HapII 418 CCGG C^CGG HhaI 227 GCGC GCG^C HinP1I 227 GCGC G^CGC HpaII 418 CCGG C^CGG Ksp632I 520 GAAGAG CTCTTC(1/4) MaeI 565 CTAG C^TAG MboI 550 GATC ^GATC MflI 549 AGATCT [GA]^GATC[CT] MluNI 432 TGGCCA TGG^CCA MscI 432 TGGCCA TGG^CCA MspI 418 CCGG C^CGG NciI 418 CCGGG CC^[GC]GG NdeI 362 CATATG CA^TATG NdeII 550 GATC ^GATC PpuMI 524 AGGACCT [GA]G^G[AT]CC[CT] Sau3AI 550 GATC ^GATC TaqI 290 TCGA T^CGA TthHB8I 290 TCGA T^CGA XhoII 549 AGATCT [GA]^GATC[CT] XmnI 292 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AcsI 63 GAATTT [GA]^AATT[CT] 272 AAATTC ApoI 63 GAATTT [GA]^AATT[CT] 272 AAATTC BseRI 381 GAGGAG GAGGAG(10/8) 384 GAGGAG BstNI 255 CCAGG CC^[AT]GG 501 CCTGG Cfr13I 443 GGCCC G^G[ACGT]CC 525 GGACC EcoRII 255 CCAGG ^CC[AT]GG 501 CCTGG HaeIII 433 GGCC GG^CC 443 GGCC MaeIII 31 GTGAC ^GT[ACGT]AC 589 GTGAC MvaI 255 CCAGG CC^[AT]GG 501 CCTGG PleI 412 GACTC GAGTC(4/5) 124 GAGTC Sau96I 443 GGCCC G^G[ACGT]CC 525 GGACC TfiI 20 GAATC G^A[AT]TC 357 GATTC Tsp45I 31 GTGAC ^GT[GC]AC 589 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 15 enzymes) # ######################################### AfaI BssKI BstF5I DdeI FokI HinfI MboII MnlI MseI NlaIII RsaI ScrFI Tru9I Tsp509I TspRI