(Circular) (Six-base) MAPSORT of: pFN21AE0940.seq Check: 6701 from: 1 to: 6082 pFN21AE0940 6082 bp With 182 enzymes: SgfI * August 16, 2010 14:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3892 278 Size: 53 83 186 936 2356 2468 Fragments arranged by size: 2468 2356 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3425 1937 Size: 1488 4594 Acc65I G'GTAC_C Cuts at: 3403 3403 Size: 6082 AclI AA'CG_TT Cuts at: 1563 4209 4582 1563 Size: 2646 373 3063 Fragments arranged by size: 3063 2646 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2909 3140 4145 5529 5797 1632 Size: 1277 231 1005 1384 268 1917 Fragments arranged by size: 1917 1384 1277 1005 268 231 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5885 AflIII A'CryG_T Cuts at: 1203 2081 2681 3184 4981 5958 1203 Size: 878 600 503 1797 977 1327 Fragments arranged by size: 1797 1327 977 878 600 503 AgeI A'CCGG_T Cuts at: 4913 4913 Size: 6082 AhdI GACnn_n'nnGTC Cuts at: 4811 4811 Size: 6082 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6082 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2142 2805 1184 Size: 958 663 4461 Fragments arranged by size: 4461 958 663 AlwNI CAG_nnn'CTG Cuts at: 2314 3825 5397 2314 Size: 1511 1572 2999 Fragments arranged by size: 2999 1572 1511 ApaLI G'TGCA_C Cuts at: 4139 5295 4139 Size: 1156 4926 ApoI r'AATT_y Cuts at: 1493 2037 2877 3046 3165 3391 3617 3769 5878 Size: 544 840 169 119 226 226 152 2109 Cuts at: 5878 1493 Size: 1697 Fragments arranged by size: 2109 1697 840 544 226 226 169 152 119 AseI AT'TA_AT Cuts at: 160 1032 4636 160 Size: 872 3604 1606 Fragments arranged by size: 3604 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6082 AvaI C'yCGr_G Cuts at: 1870 1943 3407 1870 Size: 73 1464 4545 Fragments arranged by size: 4545 1464 73 AvrII C'CTAG_G Cuts at: 2221 2221 Size: 6082 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2553 2586 2553 Size: 33 6049 BamHI G'GATC_C Cuts at: 2061 3412 3779 2061 Size: 1351 367 4364 Fragments arranged by size: 4364 1351 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3403 4858 618 Size: 325 217 627 1616 1455 1842 Fragments arranged by size: 1842 1616 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2862 3351 3401 1391 Size: 1471 489 50 4072 Fragments arranged by size: 4072 1471 489 50 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5111 BbvCI CC'TCA_GC Cuts at: 2289 2289 Size: 6082 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2630 2664 4273 4307 1903 Size: 34 50 34 609 34 1609 34 3678 Fragments arranged by size: 3678 1609 609 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3974 5184 3974 Size: 1210 4872 BclI T'GATC_A Cuts at: 1548 2500 2872 1548 Size: 952 372 4758 Fragments arranged by size: 4758 952 372 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4693 136 Size: 107 122 71 631 605 3021 1525 Fragments arranged by size: 3021 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6077 6077 Size: 6082 BlpI GC'TnA_GC Cuts at: 3499 3499 Size: 6082 Bme1580I G_kGCm'C Cuts at: 1790 2439 4143 5299 1790 Size: 649 1704 1156 2573 Fragments arranged by size: 2573 1704 1156 649 BmgBI CAC'GTC Cuts at: 1318 2440 1318 Size: 1122 4960 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3813 4771 442 Size: 416 53 83 399 24 2396 958 1753 Fragments arranged by size: 2396 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6082 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3096 4742 1239 Size: 1857 1646 2579 Fragments arranged by size: 2579 1857 1646 Bpu10I CC'TnA_GC Cuts at: 2289 2393 2289 Size: 104 5978 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4207 5072 5370 4207 Size: 865 298 4919 Fragments arranged by size: 4919 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2405 3209 4745 882 Size: 617 906 804 1536 2219 Fragments arranged by size: 2219 1536 906 804 617 BsaAI yAC'GTr Cuts at: 493 1204 2821 3873 493 Size: 711 1617 1052 2702 Fragments arranged by size: 2702 1617 1052 711 BsaBI GATnn'nnATC Cuts at: 6076 6076 Size: 6082 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3889 4271 4906 275 Size: 53 83 186 936 2356 382 635 1451 Fragments arranged by size: 2356 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2483 4515 4913 5187 5334 5745 2483 Size: 2032 398 274 147 411 2820 Fragments arranged by size: 2820 2032 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5857 Fragments arranged by size: 5857 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2125 3086 3089 1186 Size: 939 961 3 4179 Fragments arranged by size: 4179 961 939 3 BseYI C'CCAG_C Cuts at: 5285 5781 5285 Size: 496 5586 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2315 2690 1163 Size: 573 579 375 4555 Fragments arranged by size: 4555 579 573 375 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2644 3532 4293 5321 1540 Size: 275 186 643 888 761 1028 2301 Fragments arranged by size: 2301 1028 888 761 643 275 186 BsiHKAI G_wGCw'C Cuts at: 2014 2862 3351 4143 4228 5299 2014 Size: 848 489 792 85 1071 2797 Fragments arranged by size: 2797 1071 848 792 489 85 BsmI GAATG_Cn' Cuts at: 2988 3593 3686 2988 Size: 605 93 5384 Fragments arranged by size: 5384 605 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2014 2439 2862 3351 3401 4143 4228 Size: 399 224 425 423 489 50 742 85 Cuts at: 4228 5299 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 489 425 423 399 224 85 50 BspEI T'CCGG_A Cuts at: 5745 5745 Size: 6082 BspHI T'CATG_A Cuts at: 3971 5701 3971 Size: 1730 4352 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2457 3277 3437 844 Size: 1613 820 160 3489 Fragments arranged by size: 3489 1613 820 160 BsrBI CCG'CTC Cuts at: 1394 1823 3969 1394 Size: 429 2146 3507 Fragments arranged by size: 3507 2146 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4577 4751 65 Size: 1183 3329 174 1396 Fragments arranged by size: 3329 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3371 4726 4913 1700 Size: 110 1561 1355 187 2869 Fragments arranged by size: 2869 1561 1355 187 110 BsrGI T'GTAC_A Cuts at: 96 2367 96 Size: 2271 3811 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6082 BssSI C'ACGA_G Cuts at: 4142 5154 4142 Size: 1012 5070 BstAPI GCAn_nnn'nTGC Cuts at: 794 2314 794 Size: 1520 4562 BstBI TT'CG_AA Cuts at: 4886 4886 Size: 6082 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6082 BstYI r'GATC_y Cuts at: 1972 2061 3412 3779 4164 4181 5633 5719 5731 Size: 89 1351 367 385 17 1452 86 12 Cuts at: 5731 6077 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1351 385 367 346 89 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3001 3001 Size: 6082 BtgI C'CryG_G Cuts at: 513 1065 2694 513 Size: 552 1629 3901 Fragments arranged by size: 3901 1629 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5343 Fragments arranged by size: 5343 683 56 BtsI GCAGTG_nn' Cuts at: 2194 3598 4392 4412 2194 Size: 1404 794 20 3864 Fragments arranged by size: 3864 1404 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6082 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2023 2058 529 Size: 35 1459 35 4553 Fragments arranged by size: 4553 1459 35 35 DraI TTT'AAA Cuts at: 3386 3386 Size: 6082 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5089 809 Size: 507 3773 1802 Fragments arranged by size: 3773 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3529 4418 6047 Size: 54 1425 50 275 18 1699 889 1629 Cuts at: 6047 8 Size: 43 Fragments arranged by size: 1699 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3529 1537 Size: 275 1717 4090 Fragments arranged by size: 4090 1717 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2694 4012 1353 Size: 1341 1318 3423 Fragments arranged by size: 3423 1341 1318 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4669 5043 5189 387 Size: 1064 138 3080 374 146 1280 Fragments arranged by size: 3080 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2860 3349 2860 Size: 489 5593 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2909 3096 3140 4145 4742 5529 5797 Size: 393 1277 187 44 1005 597 787 268 Cuts at: 5797 1239 Size: 1524 Fragments arranged by size: 1524 1277 1005 787 597 393 268 187 44 EcoNI CCTnn'n_nnAGG Cuts at: 2143 2143 Size: 6082 EcoRI G'AATT_C Cuts at: 3391 3769 3391 Size: 378 5704 EcoRV GAT'ATC Cuts at: 3108 3108 Size: 6082 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2119 3367 2119 Size: 1248 4834 FspI TGC'GCA Cuts at: 4588 4588 Size: 6082 HaeII r_GCGC'y Cuts at: 1678 5229 1678 Size: 3551 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2039 2071 2549 2581 4723 Size: 32 135 32 467 32 478 32 2142 Cuts at: 4723 4755 4797 4829 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2142 478 467 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3426 3672 669 Size: 988 281 1488 246 3079 Fragments arranged by size: 3079 1488 988 281 246 HindIII A'AGCT_T Cuts at: 748 3152 748 Size: 2404 3678 HpaI GTT'AAC Cuts at: 3672 3672 Size: 6082 KpnI G_GTAC'C Cuts at: 3407 3407 Size: 6082 MfeI C'AATT_G Cuts at: 3681 3681 Size: 6082 MluI A'CGCG_T Cuts at: 5958 5958 Size: 6082 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5196 5380 1305 Size: 3891 184 2007 Fragments arranged by size: 3891 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4260 Fragments arranged by size: 4260 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2733 3207 4040 4399 4558 518 Size: 648 1567 474 833 359 159 2042 Fragments arranged by size: 2042 1567 833 648 474 359 159 MspA1I CmG'CkG Cuts at: 1606 2311 2326 2418 2713 3064 3498 4175 5323 Size: 705 15 92 295 351 434 677 1148 Cuts at: 5323 5568 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 705 677 434 351 295 245 92 15 NcoI C'CATG_G Cuts at: 513 1065 2694 513 Size: 552 1629 3901 Fragments arranged by size: 3901 1629 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6082 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6082 NotI GC'GGCC_GC Cuts at: 3529 3529 Size: 6082 NspI r_CATG'y Cuts at: 1132 1229 2085 2685 3188 3440 4985 1132 Size: 97 856 600 503 252 1545 2229 Fragments arranged by size: 2229 1545 856 600 503 252 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6082 PciI A'CATG_T Cuts at: 2081 2681 3184 4981 2081 Size: 600 503 1797 3182 Fragments arranged by size: 3182 1797 600 503 PflMI CCAn_nnn'nTGG Cuts at: 1108 2821 1108 Size: 1713 4369 PmeI GTTT'AAAC Cuts at: 3386 3386 Size: 6082 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4151 4183 5664 5696 4151 Size: 32 1481 32 4537 Fragments arranged by size: 4537 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6082 PsiI TTA'TAA Cuts at: 3652 3652 Size: 6082 PstI C_TGCA'G Cuts at: 830 2208 2319 3288 3434 830 Size: 1378 111 969 146 3478 Fragments arranged by size: 3478 1378 969 146 111 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6082 PvuII CAG'CTG Cuts at: 2713 3064 2713 Size: 351 5731 SacI G_AGCT'C Cuts at: 2862 3351 2862 Size: 489 5593 SalI G'TCGA_C Cuts at: 1936 3424 1936 Size: 1488 4594 SapI GCTCTTCn'nnn_ Cuts at: 2694 2694 Size: 6082 SbfI CC_TGCA'GG Cuts at: 3434 3434 Size: 6082 ScaI AGT'ACT Cuts at: 4330 6042 4330 Size: 1712 4370 SfcI C'TryA_G Cuts at: 826 1045 2204 2315 2587 3284 3430 4565 5246 Size: 219 1159 111 272 697 146 1135 681 Cuts at: 5246 5437 826 Size: 191 1471 Fragments arranged by size: 1471 1159 1135 697 681 272 219 191 146 111 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6082 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6082 SmaI CCC'GGG Cuts at: 1872 3409 1872 Size: 1537 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4186 5087 5349 820 Size: 197 926 2243 901 262 1553 Fragments arranged by size: 2243 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3873 493 Size: 3380 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6082 SphI G_CATG'C Cuts at: 1132 3440 1132 Size: 2308 3774 SspI AAT'ATT Cuts at: 5 52 4006 5 Size: 47 3954 2081 Fragments arranged by size: 3954 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6082 StyI C'CwwG_G Cuts at: 513 1065 2144 2221 2694 3137 3219 3521 513 Size: 552 1079 77 473 443 82 302 3074 Fragments arranged by size: 3074 1079 552 473 443 302 82 77 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2658 3083 3132 4109 4126 4279 4464 Size: 1255 1078 425 49 977 17 153 185 Cuts at: 4464 4909 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1078 977 445 425 185 153 49 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2367 4328 6040 Size: 274 80 33 51 1443 390 1961 1712 Cuts at: 6040 96 Size: 138 Fragments arranged by size: 1961 1712 1443 390 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2762 2794 6042 6074 2762 Size: 32 3248 32 2770 Fragments arranged by size: 3248 2770 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6082 XbaI T'CTAG_A Cuts at: 3418 3418 Size: 6082 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2818 3120 2818 Size: 302 5780 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6082 XmaI C'CCGG_G Cuts at: 1870 3407 1870 Size: 1537 4545 XmnI GAAnn'nnTTC Cuts at: 4211 4211 Size: 6082 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3890 276 Size: 53 83 186 936 2356 2468 Fragments arranged by size: 2468 2356 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI