Restriction map for
pFN21AB8607
(Circular) (Six-base) MAPSORT of: pFN21AB8607.seq Check: 7744 from: 1 to: 6763
pFN21AB8607 6763 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4573 278
Size: 53 83 186 936 3037 2468
Fragments arranged by size:
3037 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2256 4106 1937
Size: 319 1850 4594
Fragments arranged by size:
4594 1850 319
Acc65I G'GTAC_C
Cuts at: 4084 4084
Size: 6763
AclI AA'CG_TT
Cuts at: 1563 4890 5263 1563
Size: 3327 373 3063
Fragments arranged by size:
3327 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2352 3483 4826 6210 6478 1632
Size: 720 1131 1343 1384 268 1917
Fragments arranged by size:
1917 1384 1343 1131 720 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6566
AflIII A'CryG_T
Cuts at: 1203 2655 3108 3663 3768 5662 6639 1203
Size: 1452 453 555 105 1894 977 1327
Fragments arranged by size:
1894 1452 1327 977 555 453 105
AgeI A'CCGG_T
Cuts at: 5594 5594
Size: 6763
AhdI GACnn_n'nnGTC
Cuts at: 5492 5492
Size: 6763
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6763
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3806 1184
Size: 2622 4141
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2864 2896 2864
Size: 32 6731
AlwNI CAG_nnn'CTG
Cuts at: 4506 6078 4506
Size: 1572 5191
ApaI G_GGCC'C
Cuts at: 3210 3210
Size: 6763
ApaLI G'TGCA_C
Cuts at: 4820 5976 4820
Size: 1156 5607
ApoI r'AATT_y
Cuts at: 1493 2417 3977 4072 4298 4450 6559 1493
Size: 924 1560 95 226 152 2109 1697
Fragments arranged by size:
2109 1697 1560 924 226 152 95
AseI AT'TA_AT
Cuts at: 160 1032 5317 160
Size: 872 4285 1606
Fragments arranged by size:
4285 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6763
AvaI C'yCGr_G
Cuts at: 1870 1943 2799 3087 4088 1870
Size: 73 856 288 1001 4545
Fragments arranged by size:
4545 1001 856 288 73
BamHI G'GATC_C
Cuts at: 4093 4460 4093
Size: 367 6396
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2054 4084 5539 618
Size: 325 217 627 267 2030 1455 1842
Fragments arranged by size:
2030 1842 1455 627 325 267 217
BanII G_rGCy'C
Cuts at: 1391 3210 4082 1391
Size: 1819 872 4072
Fragments arranged by size:
4072 1819 872
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3825 928
Size: 971 1926 3866
Fragments arranged by size:
3866 1926 971
BbvCI CC'TCA_GC
Cuts at: 2229 2229
Size: 6763
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2867 2901 4954 4988 1903
Size: 34 50 34 846 34 2053 34 3678
Fragments arranged by size:
3678 2053 846 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2164 4655 5865 2164
Size: 2491 1210 3062
Fragments arranged by size:
3062 2491 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6763
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2732 5374 136
Size: 107 122 71 631 605 1060 2642 1525
Fragments arranged by size:
2642 1525 1060 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3043 3330 6758 3043
Size: 287 3428 3048
Fragments arranged by size:
3428 3048 287
BlpI GC'TnA_GC
Cuts at: 4180 4180
Size: 6763
Bme1580I G_kGCm'C
Cuts at: 1790 2059 3210 3785 4824 5980 1790
Size: 269 1151 575 1039 1156 2573
Fragments arranged by size:
2573 1156 1151 1039 575 269
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6763
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2654 3657 4494
Size: 416 53 83 399 24 1237 1003 837
Cuts at: 4494 5452 442
Size: 958 1753
Fragments arranged by size:
1753 1237 1003 958 837 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3158 1056
Size: 2102 4661
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3141 3543 5423 1239
Size: 1902 402 1880 2579
Fragments arranged by size:
2579 1902 1880 402
Bpu10I CC'TnA_GC
Cuts at: 2229 2358 3139 2229
Size: 129 781 5853
Fragments arranged by size:
5853 781 129
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4888 5753 6051 4888
Size: 865 298 5600
Fragments arranged by size:
5600 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3117 5426 882
Size: 617 1618 2309 2219
Fragments arranged by size:
2309 2219 1618 617
BsaAI yAC'GTr
Cuts at: 493 1204 2662 4554 493
Size: 711 1458 1892 2702
Fragments arranged by size:
2702 1892 1458 711
BsaBI GATnn'nnATC
Cuts at: 6757 6757
Size: 6763
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2019 4570 4952 5587
Size: 53 83 186 936 486 2551 382 635
Cuts at: 5587 275
Size: 1451
Fragments arranged by size:
2551 1451 936 635 486 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3576 5196 5594 5868 6015 6426 3576
Size: 1620 398 274 147 411 3913
Fragments arranged by size:
3913 1620 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2270 2300 2864 2894 2931
Size: 30 165 30 867 30 564 30 37
Cuts at: 2931 2961 3838 3868 1178
Size: 30 877 30 4073
Fragments arranged by size:
4073 877 867 564 165 37 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2598 3610 3744 3859 3951 1186
Size: 1412 1012 134 115 92 3998
Fragments arranged by size:
3998 1412 1012 134 115 92
BseYI C'CCAG_C
Cuts at: 2072 2294 5966 6462 2072
Size: 222 3672 496 2373
Fragments arranged by size:
3672 2373 496 222
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6190
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4213 4974 6002 1540
Size: 275 186 2212 761 1028 2301
Fragments arranged by size:
2301 2212 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2099 3883 4824 4909 5980 2099
Size: 1784 941 85 1071 2882
Fragments arranged by size:
2882 1784 1071 941 85
BsmI GAATG_Cn'
Cuts at: 2857 4274 4367 2857
Size: 1417 93 5253
Fragments arranged by size:
5253 1417 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2059 2099 3210 3785 3883 4082 4824
Size: 399 269 40 1111 575 98 199 742
Cuts at: 4824 4909 5980 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1111 1071 742 575 399 269 199
98 85 40
BspEI T'CCGG_A
Cuts at: 6426 6426
Size: 6763
BspHI T'CATG_A
Cuts at: 3417 4652 6382 3417
Size: 1235 1730 3798
Fragments arranged by size:
3798 1730 1235
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2073 3159 4118 844
Size: 1229 1086 959 3489
Fragments arranged by size:
3489 1229 1086 959
BsrBI CCG'CTC
Cuts at: 1394 1823 4650 1394
Size: 429 2827 3507
Fragments arranged by size:
3507 2827 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5258 5432 65
Size: 1183 4010 174 1396
Fragments arranged by size:
4010 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2051 2495 5407 5594 1700
Size: 110 241 444 2912 187 2869
Fragments arranged by size:
2912 2869 444 241 187 110
BsrGI T'GTAC_A
Cuts at: 96 3551 3666 96
Size: 3455 115 3193
Fragments arranged by size:
3455 3193 115
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6763
BssSI C'ACGA_G
Cuts at: 4823 5835 4823
Size: 1012 5751
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2041 794
Size: 1247 5516
BstBI TT'CG_AA
Cuts at: 5567 5567
Size: 6763
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6763
BstXI CCAn_nnnn'nTGG
Cuts at: 3773 3773
Size: 6763
BstYI r'GATC_y
Cuts at: 1972 2290 2760 3043 3330 4093 4460 4845 4862
Size: 318 470 283 287 763 367 385 17
Cuts at: 4862 6314 6400 6412 6758 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 763 470 385 367 346 318
287 283 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2132 2721 2730 2898 3015 513
Size: 552 1067 589 9 168 117 4261
Fragments arranged by size:
4261 1067 589 552 168 117 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2452 487
Size: 683 56 1226 4798
Fragments arranged by size:
4798 1226 683 56
BtsI GCAGTG_nn'
Cuts at: 2980 3745 4279 5073 5093 2980
Size: 765 534 794 20 4650
Fragments arranged by size:
4650 794 765 534 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6763
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6728
DraI TTT'AAA
Cuts at: 2142 3270 4067 2142
Size: 1128 797 4838
Fragments arranged by size:
4838 1128 797
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5770 809
Size: 507 4454 1802
Fragments arranged by size:
4454 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4210 5099 6728
Size: 54 1425 50 275 18 2380 889 1629
Cuts at: 6728 8
Size: 43
Fragments arranged by size:
2380 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4210 1537
Size: 275 2398 4090
Fragments arranged by size:
4090 2398 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2085 3300 3822 3844 4693 1353
Size: 732 1215 522 22 849 3423
Fragments arranged by size:
3423 1215 849 732 522 22
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5350 5724 5870 387
Size: 1064 138 3761 374 146 1280
Fragments arranged by size:
3761 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2352 3141 3483 3543 4826 5423 6210
Size: 393 720 789 342 60 1283 597 787
Cuts at: 6210 6478 1239
Size: 268 1524
Fragments arranged by size:
1524 1283 789 787 720 597 393 342
268 60
EcoNI CCTnn'n_nnAGG
Cuts at: 2819 2870 3726 2819
Size: 51 856 5856
Fragments arranged by size:
5856 856 51
EcoO109I rG'GnC_Cy
Cuts at: 2076 3207 3989 2076
Size: 1131 782 4850
Fragments arranged by size:
4850 1131 782
EcoRI G'AATT_C
Cuts at: 4072 4450 4072
Size: 378 6385
FspI TGC'GCA
Cuts at: 5269 5269
Size: 6763
HaeII r_GCGC'y
Cuts at: 1678 5910 1678
Size: 4232 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2270 2302 3787 3819 5404
Size: 32 135 32 698 32 1485 32 1585
Cuts at: 5404 5436 5478 5510 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1585 1485 698 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2718 4107 4353 669
Size: 988 281 780 1389 246 3079
Fragments arranged by size:
3079 1389 988 780 281 246
HindIII A'AGCT_T
Cuts at: 748 2332 748
Size: 1584 5179
HpaI GTT'AAC
Cuts at: 4353 4353
Size: 6763
KpnI G_GTAC'C
Cuts at: 4088 4088
Size: 6763
MfeI C'AATT_G
Cuts at: 3479 4362 3479
Size: 883 5880
MluI A'CGCG_T
Cuts at: 6639 6639
Size: 6763
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2560 3062 3324 5877 6061 1305
Size: 1255 502 262 2553 184 2007
Fragments arranged by size:
2553 2007 1255 502 262 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4941
Fragments arranged by size:
4941 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2165 2535 2660 3771 4721 5080 5239
Size: 648 999 370 125 1111 950 359 159
Cuts at: 5239 518
Size: 2042
Fragments arranged by size:
2042 1111 999 950 648 370 359 159
125
MspA1I CmG'CkG
Cuts at: 1606 2072 2302 2376 2553 3027 3585 3897 4179
Size: 466 230 74 177 474 558 312 282
Cuts at: 4179 4856 6004 6249 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 558 474 466 312 282
245 230 177 74
NcoI C'CATG_G
Cuts at: 513 1065 2730 2898 513
Size: 552 1665 168 4378
Fragments arranged by size:
4378 1665 552 168
NdeI CA'TA_TG
Cuts at: 387 3684 387
Size: 3297 3466
NheI G'CTAG_C
Cuts at: 1052 3154 1052
Size: 2102 4661
NotI GC'GGCC_GC
Cuts at: 4210 4210
Size: 6763
NspI r_CATG'y
Cuts at: 1132 1229 2659 3112 3667 3772 4121 5666 1132
Size: 97 1430 453 555 105 349 1545 2229
Fragments arranged by size:
2229 1545 1430 555 453 349 105 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6763
PciI A'CATG_T
Cuts at: 2655 3108 3663 3768 5662 2655
Size: 453 555 105 1894 3756
Fragments arranged by size:
3756 1894 555 453 105
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2695 1108
Size: 1587 5176
PfoI T'CCnGG_A
Cuts at: 2688 2688
Size: 6763
PmeI GTTT'AAAC
Cuts at: 4067 4067
Size: 6763
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2864 2896 4832 4864 6345 6377 2864
Size: 32 1936 32 1481 32 3250
Fragments arranged by size:
3250 1936 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2076 3989 2076
Size: 1913 4850
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6763
PsiI TTA'TAA
Cuts at: 4333 4333
Size: 6763
PspOMI G'GGCC_C
Cuts at: 3206 3206
Size: 6763
PstI C_TGCA'G
Cuts at: 830 2046 4115 830
Size: 1216 2069 3478
Fragments arranged by size:
3478 2069 1216
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6763
PvuII CAG'CTG
Cuts at: 2302 2376 3027 3585 3897 2302
Size: 74 651 558 312 5168
Fragments arranged by size:
5168 651 558 312 74
SalI G'TCGA_C
Cuts at: 1936 4105 1936
Size: 2169 4594
SbfI CC_TGCA'GG
Cuts at: 4115 4115
Size: 6763
ScaI AGT'ACT
Cuts at: 5011 6723 5011
Size: 1712 5051
SfcI C'TryA_G
Cuts at: 826 1045 2042 3132 4111 5246 5927 6118 826
Size: 219 997 1090 979 1135 681 191 1471
Fragments arranged by size:
1471 1135 1090 997 979 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6763
SgrAI Cr'CCGG_yG
Cuts at: 1700 2051 1700
Size: 351 6412
SmaI CCC'GGG
Cuts at: 1872 4090 1872
Size: 2218 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4867 5768 6030 820
Size: 197 926 2924 901 262 1553
Fragments arranged by size:
2924 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4554 493
Size: 4061 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6763
SphI G_CATG'C
Cuts at: 1132 4121 1132
Size: 2989 3774
SspI AAT'ATT
Cuts at: 5 52 4687 5
Size: 47 4635 2081
Fragments arranged by size:
4635 2081 47
StuI AGG'CCT
Cuts at: 1517 3677 3721 1517
Size: 2160 44 4559
Fragments arranged by size:
4559 2160 44
StyI C'CwwG_G
Cuts at: 513 1065 2730 2898 2934 3353 3405 4202 513
Size: 552 1665 168 36 419 52 797 3074
Fragments arranged by size:
3074 1665 797 552 419 168 52 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4790 4807 4960 5145 5590 325
Size: 1255 3210 17 153 185 445 1498
Fragments arranged by size:
3210 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2440 3530 3551
Size: 274 80 33 51 1443 463 1090 21
Cuts at: 3551 3666 5009 6721 96
Size: 115 1343 1712 138
Fragments arranged by size:
1712 1443 1343 1090 463 274 138 115
80 51 33 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3838 3870 6723 6755 3838
Size: 32 2853 32 3846
Fragments arranged by size:
3846 2853 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2780 1316
Size: 1464 5299
XbaI T'CTAG_A
Cuts at: 4099 4099
Size: 6763
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2728 3360 3689 2728
Size: 632 329 5802
Fragments arranged by size:
5802 632 329
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6763
XmaI C'CCGG_G
Cuts at: 1870 4088 1870
Size: 2218 4545
XmnI GAAnn'nnTTC
Cuts at: 2684 3930 4892 2684
Size: 1246 962 4555
Fragments arranged by size:
4555 1246 962
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4571 276
Size: 53 83 186 936 3037 2468
Fragments arranged by size:
3037 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI