################################## # NO CUT ENZYMES ( 107 enzymes) # ################################## AatII Acc65I AclI AflII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BamHI BbrPI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsiWI BsmBI BsmI Bsp1407I BspDI BsrBI BsrDI BsrGI BssHII BstAPI BstBI BstEII BstPI CelII ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III EcoNI EcoO65I EcoT14I EcoT22I FbaI FseI FspI HindIII KpnI MamI MfeI MluI MluNI MscI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SalI SapI SbfI SexAI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tth111I Van91I XbaI XcmI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccI 852 GTATAC GT^[AC][GT]AC AocI 1631 CCTCAGG CC^T[ACGT]AGG ApaI 13 GGGCCC GGGCC^C BsiEI 41 CGGCCG CG[GA][CT]^CG BsmAI 1357 GTCTC GTCTC(1/5) BspHI 627 TCATGA T^CATGA BspLU11I 1139 ACATGT A^CATGT BspMI 679 ACCTGC ACCTGC(4/8) Bst1107I 852 GTATAC GTA^TAC BstYI 711 GGATCT [GA]^GATC[CT] BstZ17I 852 GTATAC GTA^TAC Bsu36I 1631 CCTCAGG CC^T[ACGT]AGG DraII 13 GGGCCCC [GA]G^G[ACGT]CC[CT] EaeI 41 CGGCCG [CT]^GGCC[GA] EagI 41 CGGCCG C^GGCCG EarI 1274 CTCTTC CTCTTC(1/4) EclXI 41 CGGCCG C^GGCCG Eco52I 41 CGGCCG C^GGCCG Eco81I 1631 CCTCAGG CC^T[ACGT]AGG EcoO109I 13 GGGCCCC [GA]G^G[ACGT]CC[CT] EcoRI 1327 GAATTC G^AATTC EcoRV 959 GATATC GAT^ATC HgaI 66 GACGC GACGC(5/10) HpaI 915 GTTAAC GTT^AAC Ksp632I 1274 CTCTTC CTCTTC(1/4) MflI 711 GGATCT [GA]^GATC[CT] NaeI 284 GCCGGC GCC^GGC NgoMIV 284 GCCGGC G^CCGGC NspI 1139 ACATGT [GA]CATG^[CT] PaeR7I 520 CTCGAG C^TCGAG PstI 303 CTGCAG CTGCA^G PvuII 1249 CAGCTG CAG^CTG RcaI 627 TCATGA T^CATGA ScaI 524 AGTACT AGT^ACT SfcI 303 CTGCAG C^T[GA][CT]AG XhoI 520 CTCGAG C^TCGAG XhoII 711 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AccIII 202 TCCGGA T^CCGGA 228 TCCGGA AcsI 880 AAATTC [GA]^AATT[CT] 1327 GAATTC AflIII 892 ACACGT A^C[GA][CT]GT 1139 ACATGT ApoI 880 AAATTC [GA]^AATT[CT] 1327 GAATTC Asp700I 1037 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1454 GAATGATTTC AvaII 414 GGACC G^G[AT]CC 1081 GGTCC BbeI 172 GGCGCC GGCGC^C 505 GGCGCC BbsI 631 GAAGAC GAAGAC(2/6) 511 GTCTTC BbvCI 486 CCTCAGC CCTCAGC(-5/-2) 55 GCTGAGG BpuAI 631 GAAGAC GAAGAC(2/6) 511 GTCTTC BsaWI 202 TCCGGA [AT]^CCGG[AT] 228 TCCGGA BseAI 202 TCCGGA T^CCGGA 228 TCCGGA BseRI 58 GAGGAG GAGGAG(10/8) 1306 GAGGAG BsgI 1252 CTGCAC GTGCAG(16/14) 1509 GTGCAG BspEI 202 TCCGGA T^CCGGA 228 TCCGGA BsrFI 284 GCCGGC [GA]^CCGG[CT] 665 ACCGGC BsrI 1345 CCAGT ACTGG(1/-1) 1544 CCAGT BssSI 73 CACGAG CACGAG(-5/-1) 1145 CACGAG BstXI 18 CCATAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1258 CCAAAAGGCTGG Cfr10I 284 GCCGGC [GA]^CCGG[CT] 665 ACCGGC HincII 915 GTTAAC GT[CT]^[GA]AC 1522 GTTGAC HindII 915 GTTAAC GT[CT]^[GA]AC 1522 GTTGAC KasI 172 GGCGCC G^GCGCC 505 GGCGCC KspI 399 CCGCGG CCGC^GG 470 CCGCGG MroI 202 TCCGGA T^CCGGA 228 TCCGGA NarI 172 GGCGCC GG^CGCC 505 GGCGCC SacI 224 GAGCTC GAGCT^C 1340 GAGCTC SacII 399 CCGCGG CCGC^GG 470 CCGCGG SfaNI 127 GATGC GCATC(5/9) 1235 GCATC SfiI 322 GGCCGACACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 343 GGCCTGGGCGGCC SfoI 172 GGCGCC GGC^GCC 505 GGCGCC SmlI 520 CTCGAG C^T[CT][GA]AG 1287 CTCAAG TaqI 521 TCGA T^CGA 776 TCGA TfiI 1117 GATTC G^A[AT]TC 1482 GATTC TthHB8I 521 TCGA T^CGA 776 TCGA XmnI 1037 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1454 GAATGATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 78 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AspHI AvaI BanI BanII BbvI BcnI BfaI BglI BmyI BpmI BsaHI BsaJI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DsaI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SmaI Tru9I TseI Tsp45I Tsp509I TspRI XmaCI XmaI