(Circular) (Six-base) MAPSORT of: pFN21AE3239.seq Check: 6188 from: 1 to: 6382 pFN21AE3239 6382 bp With 182 enzymes: SgfI * March 28, 2012 12:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4192 278 Size: 53 83 186 936 2656 2468 Fragments arranged by size: 2656 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2858 3725 1937 Size: 921 867 4594 Fragments arranged by size: 4594 921 867 Acc65I G'GTAC_C Cuts at: 3703 3703 Size: 6382 AclI AA'CG_TT Cuts at: 1563 4509 4882 1563 Size: 2946 373 3063 Fragments arranged by size: 3063 2946 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2595 2646 3228 3427 4445 5829 6097 1632 Size: 963 51 582 199 1018 1384 268 1917 Fragments arranged by size: 1917 1384 1018 963 582 268 199 51 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6185 AflIII A'CryG_T Cuts at: 1203 2897 3144 5281 6258 1203 Size: 1694 247 2137 977 1327 Fragments arranged by size: 2137 1694 1327 977 247 AgeI A'CCGG_T Cuts at: 5213 5213 Size: 6382 AhdI GACnn_n'nnGTC Cuts at: 5111 5111 Size: 6382 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6382 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6382 AlwNI CAG_nnn'CTG Cuts at: 4125 5697 4125 Size: 1572 4810 ApaI G_GGCC'C Cuts at: 2022 2022 Size: 6382 ApaLI G'TGCA_C Cuts at: 4439 5595 4439 Size: 1156 5226 ApoI r'AATT_y Cuts at: 1493 2885 3332 3691 3917 4069 6178 1493 Size: 1392 447 359 226 152 2109 1697 Fragments arranged by size: 2109 1697 1392 447 359 226 152 AseI AT'TA_AT Cuts at: 160 1032 4936 160 Size: 872 3904 1606 Fragments arranged by size: 3904 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6382 AvaI C'yCGr_G Cuts at: 1870 1943 2123 2257 2297 2525 3707 1870 Size: 73 180 134 40 228 1182 4545 Fragments arranged by size: 4545 1182 228 180 134 73 40 BamHI G'GATC_C Cuts at: 3712 4079 3712 Size: 367 6015 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2177 2510 3642 3703 5158 Size: 325 217 627 390 333 1132 61 1455 Cuts at: 5158 618 Size: 1842 Fragments arranged by size: 1842 1455 1132 627 390 333 325 217 61 BanII G_rGCy'C Cuts at: 1391 2022 2233 3349 3701 1391 Size: 631 211 1116 352 4072 Fragments arranged by size: 4072 1116 631 352 211 BbeI G_GCGC'C Cuts at: 2181 2514 2181 Size: 333 6049 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2509 2643 928 Size: 971 610 134 4667 Fragments arranged by size: 4667 971 610 134 BbvCI CC'TCA_GC Cuts at: 2061 2492 2061 Size: 431 5951 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4573 4607 1903 Size: 34 2636 34 3678 Fragments arranged by size: 3678 2636 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4274 5484 4274 Size: 1210 5172 BclI T'GATC_A Cuts at: 1548 1548 Size: 6382 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2175 2334 2355 Size: 107 122 71 631 605 503 159 21 Cuts at: 2355 4993 136 Size: 2638 1525 Fragments arranged by size: 2638 1525 631 605 503 159 122 107 71 21 BglII A'GATC_T Cuts at: 6377 6377 Size: 6382 BlpI GC'TnA_GC Cuts at: 3799 3799 Size: 6382 Bme1580I G_kGCm'C Cuts at: 1790 2022 4443 5599 1790 Size: 232 2421 1156 2573 Fragments arranged by size: 2573 2421 1156 232 BmgBI CAC'GTC Cuts at: 1318 3246 1318 Size: 1928 4454 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4113 5071 442 Size: 416 53 83 399 24 2696 958 1753 Fragments arranged by size: 2696 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6382 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2052 2247 3333 5042 1239 Size: 813 195 1086 1709 2579 Fragments arranged by size: 2579 1709 1086 813 195 Bpu10I CC'TnA_GC Cuts at: 2061 2492 2061 Size: 431 5951 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3277 4507 5372 5670 3277 Size: 1230 865 298 3989 Fragments arranged by size: 3989 1230 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5045 882 Size: 617 3546 2219 Fragments arranged by size: 3546 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4173 493 Size: 711 2969 2702 Fragments arranged by size: 2969 2702 711 BsaBI GATnn'nnATC Cuts at: 6376 6376 Size: 6382 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2072 2178 2511 4189 Size: 53 83 186 936 539 106 333 1678 Cuts at: 4189 4571 5206 275 Size: 382 635 1451 Fragments arranged by size: 1678 1451 936 635 539 382 333 186 106 83 53 BsaWI w'CCGG_w Cuts at: 2207 2233 4815 5213 5487 5634 6045 2207 Size: 26 2582 398 274 147 411 2544 Fragments arranged by size: 2582 2544 411 398 274 147 26 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3336 3366 1178 Size: 30 165 30 1933 30 4194 Fragments arranged by size: 4194 1933 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2078 3326 1186 Size: 892 1248 4242 Fragments arranged by size: 4242 1248 892 BseYI C'CCAG_C Cuts at: 2766 3270 5585 6081 2766 Size: 504 2315 496 3067 Fragments arranged by size: 3067 2315 504 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3242 3535 1163 Size: 573 1506 293 4010 Fragments arranged by size: 4010 1506 573 293 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2049 3832 4593 5621 1540 Size: 275 186 48 1783 761 1028 2301 Fragments arranged by size: 2301 1783 1028 761 275 186 48 BsiHKAI G_wGCw'C Cuts at: 2233 3293 3349 4443 4528 5599 2233 Size: 1060 56 1094 85 1071 3016 Fragments arranged by size: 3016 1094 1071 1060 85 56 BsmI GAATG_Cn' Cuts at: 3893 3986 3893 Size: 93 6289 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2233 3293 3349 3701 4443 4528 Size: 399 232 211 1060 56 352 742 85 Cuts at: 4528 5599 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 1060 742 399 352 232 211 85 56 BspEI T'CCGG_A Cuts at: 2207 2233 6045 2207 Size: 26 3812 2544 Fragments arranged by size: 3812 2544 26 BspHI T'CATG_A Cuts at: 2632 4271 6001 2632 Size: 1639 1730 3013 Fragments arranged by size: 3013 1730 1639 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2693 3737 844 Size: 1849 1044 3489 Fragments arranged by size: 3489 1849 1044 BsrBI CCG'CTC Cuts at: 1394 1823 4269 1394 Size: 429 2446 3507 Fragments arranged by size: 3507 2446 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4877 5051 65 Size: 1183 3629 174 1396 Fragments arranged by size: 3629 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2289 2670 5026 5213 1700 Size: 110 479 381 2356 187 2869 Fragments arranged by size: 2869 2356 479 381 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6382 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6382 BssSI C'ACGA_G Cuts at: 2078 3150 4442 5454 2078 Size: 1072 1292 1012 3006 Fragments arranged by size: 3006 1292 1072 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6382 BstBI TT'CG_AA Cuts at: 5186 5186 Size: 6382 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6382 BstXI CCAn_nnnn'nTGG Cuts at: 2030 3270 2030 Size: 1240 5142 BstYI r'GATC_y Cuts at: 1972 2716 3712 4079 4464 4481 5933 6019 6031 Size: 744 996 367 385 17 1452 86 12 Cuts at: 6031 6377 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 996 744 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2859 2859 Size: 6382 Bsu36I CC'TnA_GG Cuts at: 3637 3637 Size: 6382 BtgI C'CryG_G Cuts at: 513 1065 2404 2475 2506 3229 513 Size: 552 1339 71 31 723 3666 Fragments arranged by size: 3666 1339 723 552 71 31 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2169 487 Size: 683 56 943 4700 Fragments arranged by size: 4700 943 683 56 BtsI GCAGTG_nn' Cuts at: 3523 3898 4692 4712 3523 Size: 375 794 20 5193 Fragments arranged by size: 5193 794 375 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6382 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6347 DraI TTT'AAA Cuts at: 3686 3686 Size: 6382 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5389 809 Size: 507 4073 1802 Fragments arranged by size: 4073 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 3829 4718 Size: 54 1425 50 275 18 216 1783 889 Cuts at: 4718 6347 8 Size: 1629 43 Fragments arranged by size: 1783 1629 1425 889 275 216 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2046 3829 1537 Size: 275 234 1783 4090 Fragments arranged by size: 4090 1783 275 234 EarI CTCTTCn'nnn_ Cuts at: 1353 3285 4312 1353 Size: 1932 1027 3423 Fragments arranged by size: 3423 1932 1027 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2242 4969 5343 5489 387 Size: 1064 138 653 2727 374 146 1280 Fragments arranged by size: 2727 1280 1064 653 374 146 138 EcoICRI GAG'CTC Cuts at: 2231 3347 2231 Size: 1116 5266 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2052 2247 2595 2646 3228 3333 3427 Size: 393 420 195 348 51 582 105 94 Cuts at: 3427 4445 5042 5829 6097 1239 Size: 1018 597 787 268 1524 Fragments arranged by size: 1524 1018 787 597 582 420 393 348 268 195 105 94 51 EcoO109I rG'GnC_Cy Cuts at: 2019 2019 Size: 6382 EcoRI G'AATT_C Cuts at: 3332 3691 4069 3332 Size: 359 378 5645 Fragments arranged by size: 5645 378 359 EcoRV GAT'ATC Cuts at: 2966 2966 Size: 6382 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2600 2905 3063 2600 Size: 305 158 5919 Fragments arranged by size: 5919 305 158 FspI TGC'GCA Cuts at: 4888 4888 Size: 6382 HaeII r_GCGC'y Cuts at: 1678 2181 2367 2514 5529 1678 Size: 503 186 147 3015 2531 Fragments arranged by size: 3015 2531 503 186 147 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2322 2354 2582 2614 3556 Size: 32 135 32 750 32 228 32 942 Cuts at: 3556 3588 5023 5055 5097 5129 1373 Size: 32 1435 32 42 32 2626 Fragments arranged by size: 2626 1435 942 750 228 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2922 3529 3726 3972 669 Size: 988 281 984 607 197 246 3079 Fragments arranged by size: 3079 988 984 607 281 246 197 HindIII A'AGCT_T Cuts at: 748 748 Size: 6382 HpaI GTT'AAC Cuts at: 2922 3972 2922 Size: 1050 5332 KasI G'GCGC_C Cuts at: 2177 2510 2177 Size: 333 6049 KpnI G_GTAC'C Cuts at: 3707 3707 Size: 6382 MfeI C'AATT_G Cuts at: 3981 3981 Size: 6382 MluI A'CGCG_T Cuts at: 6258 6258 Size: 6382 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3641 5496 5680 1305 Size: 2336 1855 184 2007 Fragments arranged by size: 2336 2007 1855 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4560 Fragments arranged by size: 4560 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2912 3117 3480 3588 4340 4699 4858 Size: 648 1746 205 363 108 752 359 159 Cuts at: 4858 518 Size: 2042 Fragments arranged by size: 2042 1746 752 648 363 359 205 159 108 MspA1I CmG'CkG Cuts at: 1606 2406 2477 3256 3798 4475 5623 5868 1606 Size: 800 71 779 542 677 1148 245 2120 Fragments arranged by size: 2120 1148 800 779 677 542 245 71 NaeI GCC'GGC Cuts at: 2291 2291 Size: 6382 NarI GG'CG_CC Cuts at: 2178 2511 2178 Size: 333 6049 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5830 NdeI CA'TA_TG Cuts at: 387 387 Size: 6382 NgoMIV G'CCGG_C Cuts at: 2289 2289 Size: 6382 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6382 NotI GC'GGCC_GC Cuts at: 3829 3829 Size: 6382 NspI r_CATG'y Cuts at: 1132 1229 3148 3740 5285 1132 Size: 97 1919 592 1545 2229 Fragments arranged by size: 2229 1919 1545 592 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6382 PciI A'CATG_T Cuts at: 3144 5281 3144 Size: 2137 4245 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6382 PmeI GTTT'AAAC Cuts at: 3686 3686 Size: 6382 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4451 4483 5964 5996 4451 Size: 32 1481 32 4837 Fragments arranged by size: 4837 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6382 PsiI TTA'TAA Cuts at: 3952 3952 Size: 6382 PspOMI G'GGCC_C Cuts at: 2018 2018 Size: 6382 PspXI vC'TCGA_Gb Cuts at: 2525 2525 Size: 6382 PstI C_TGCA'G Cuts at: 830 2312 3734 830 Size: 1482 1422 3478 Fragments arranged by size: 3478 1482 1422 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6382 PvuII CAG'CTG Cuts at: 3256 3256 Size: 6382 SacI G_AGCT'C Cuts at: 2233 3349 2233 Size: 1116 5266 SacII CC_GC'GG Cuts at: 2407 2478 2407 Size: 71 6311 SalI G'TCGA_C Cuts at: 1936 3724 1936 Size: 1788 4594 SbfI CC_TGCA'GG Cuts at: 3734 3734 Size: 6382 ScaI AGT'ACT Cuts at: 2531 4630 6342 2531 Size: 2099 1712 2571 Fragments arranged by size: 2571 2099 1712 SfcI C'TryA_G Cuts at: 826 1045 2308 3730 4865 5546 5737 826 Size: 219 1263 1422 1135 681 191 1471 Fragments arranged by size: 1471 1422 1263 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2334 2355 2334 Size: 21 6361 SfoI GGC'GCC Cuts at: 2179 2512 2179 Size: 333 6049 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6382 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6382 SmaI CCC'GGG Cuts at: 1872 2125 2259 2299 3709 1872 Size: 253 134 40 1410 4545 Fragments arranged by size: 4545 1410 253 134 40 SmlI C'TyrA_G Cuts at: 820 1017 1943 2525 3292 4486 5387 5649 820 Size: 197 926 582 767 1194 901 262 1553 Fragments arranged by size: 1553 1194 926 901 767 582 262 197 SnaBI TAC'GTA Cuts at: 493 4173 493 Size: 3680 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6382 SphI G_CATG'C Cuts at: 1132 3740 1132 Size: 2608 3774 SrfI GCCC'GGGC Cuts at: 2299 2299 Size: 6382 SspI AAT'ATT Cuts at: 5 52 4306 5 Size: 47 4254 2081 Fragments arranged by size: 4254 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6382 StyI C'CwwG_G Cuts at: 513 1065 3821 513 Size: 552 2756 3074 Fragments arranged by size: 3074 2756 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2519 2758 2859 3276 4409 4426 4579 Size: 1255 939 239 101 417 1133 17 153 Cuts at: 4579 4764 5209 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1133 939 445 417 239 185 153 101 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2529 3317 3576 Size: 274 80 33 51 1443 552 788 259 Cuts at: 3576 4628 6340 96 Size: 1052 1712 138 Fragments arranged by size: 1712 1443 1052 788 552 274 259 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3336 3368 6342 6374 3336 Size: 32 2974 32 3344 Fragments arranged by size: 3344 2974 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6382 XbaI T'CTAG_A Cuts at: 3718 3718 Size: 6382 XhoI C'TCGA_G Cuts at: 1943 2525 1943 Size: 582 5800 XmaI C'CCGG_G Cuts at: 1870 2123 2257 2297 3707 1870 Size: 253 134 40 1410 4545 Fragments arranged by size: 4545 1410 253 134 40 XmnI GAAnn'nnTTC Cuts at: 3046 3463 4511 3046 Size: 417 1048 4917 Fragments arranged by size: 4917 1048 417 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4190 276 Size: 53 83 186 936 2656 2468 Fragments arranged by size: 2656 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI DraIII EcoNI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PsrI RsrII SanDI SapI SexAI SwaI XcmI