(Linear) (Six-base) MAPSORT of: KIEE3239.seq Check: 673 from: 1 to: 1677 KIEE3239.orf With 182 enzymes: SgfI * March 22, 2012 16:26 .. AccI GT'mk_AC Cuts at: 0 853 1677 Size: 853 824 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 590 641 1223 1422 1677 Size: 590 51 582 199 255 Fragments arranged by size: 590 582 255 199 51 AflIII A'CryG_T Cuts at: 0 892 1139 1677 Size: 892 247 538 Fragments arranged by size: 892 538 247 ApaI G_GGCC'C Cuts at: 0 17 1677 Size: 17 1660 ApoI r'AATT_y Cuts at: 0 880 1327 1677 Size: 880 447 350 Fragments arranged by size: 880 447 350 AvaI C'yCGr_G Cuts at: 0 118 252 292 520 1677 Size: 118 134 40 228 1157 Fragments arranged by size: 1157 228 134 118 40 BanI G'GyrC_C Cuts at: 0 172 505 1637 1677 Size: 172 333 1132 40 Fragments arranged by size: 1132 333 172 40 BanII G_rGCy'C Cuts at: 0 17 228 1344 1677 Size: 17 211 1116 333 Fragments arranged by size: 1116 333 211 17 BbeI G_GCGC'C Cuts at: 0 176 509 1677 Size: 176 333 1168 Fragments arranged by size: 1168 333 176 BbsI GAAGACnn'nnnn_ Cuts at: 0 504 638 1677 Size: 504 134 1039 Fragments arranged by size: 1039 504 134 BbvCI CC'TCA_GC Cuts at: 0 56 487 1677 Size: 56 431 1190 Fragments arranged by size: 1190 431 56 BglI GCCn_nnn'nGGC Cuts at: 0 170 329 350 1677 Size: 170 159 21 1327 Fragments arranged by size: 1327 170 159 21 Bme1580I G_kGCm'C Cuts at: 0 17 1677 Size: 17 1660 BmgBI CAC'GTC Cuts at: 0 1241 1677 Size: 1241 436 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 47 242 1328 1677 Size: 47 195 1086 349 Fragments arranged by size: 1086 349 195 47 Bpu10I CC'TnA_GC Cuts at: 0 56 487 1677 Size: 56 431 1190 Fragments arranged by size: 1190 431 56 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1272 1677 Size: 1272 405 BsaHI Gr'CG_yC Cuts at: 0 67 173 506 1677 Size: 67 106 333 1171 Fragments arranged by size: 1171 333 106 67 BsaWI w'CCGG_w Cuts at: 0 202 228 1677 Size: 202 26 1449 Fragments arranged by size: 1449 202 26 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1331 1361 1677 Size: 1331 30 316 Fragments arranged by size: 1331 316 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 73 1321 1677 Size: 73 1248 356 Fragments arranged by size: 1248 356 73 BseYI C'CCAG_C Cuts at: 0 761 1265 1677 Size: 761 504 412 Fragments arranged by size: 761 504 412 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1237 1530 1677 Size: 1237 293 147 Fragments arranged by size: 1237 293 147 BsiEI CG_ry'CG Cuts at: 0 44 1677 Size: 44 1633 BsiHKAI G_wGCw'C Cuts at: 0 228 1288 1344 1677 Size: 228 1060 56 333 Fragments arranged by size: 1060 333 228 56 Bsp1286I G_dGCh'C Cuts at: 0 17 228 1288 1344 1677 Size: 17 211 1060 56 333 Fragments arranged by size: 1060 333 211 56 17 BspEI T'CCGG_A Cuts at: 0 202 228 1677 Size: 202 26 1449 Fragments arranged by size: 1449 202 26 BspHI T'CATG_A Cuts at: 0 627 1677 Size: 627 1050 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 688 1677 Size: 688 989 BsrFI r'CCGG_y Cuts at: 0 284 665 1677 Size: 284 381 1012 Fragments arranged by size: 1012 381 284 BssSI C'ACGA_G Cuts at: 0 73 1145 1677 Size: 73 1072 532 Fragments arranged by size: 1072 532 73 BstXI CCAn_nnnn'nTGG Cuts at: 0 25 1265 1677 Size: 25 1240 412 Fragments arranged by size: 1240 412 25 BstYI r'GATC_y Cuts at: 0 711 1677 Size: 711 966 BstZ17I GTA'TAC Cuts at: 0 854 1677 Size: 854 823 Bsu36I CC'TnA_GG Cuts at: 0 1632 1677 Size: 1632 45 BtgI C'CryG_G Cuts at: 0 399 470 501 1224 1677 Size: 399 71 31 723 453 Fragments arranged by size: 723 453 399 71 31 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 164 1677 Size: 164 1513 BtsI GCAGTG_nn' Cuts at: 0 1518 1677 Size: 1518 159 EaeI y'GGCC_r Cuts at: 0 41 1677 Size: 41 1636 EagI C'GGCC_G Cuts at: 0 41 1677 Size: 41 1636 EarI CTCTTCn'nnn_ Cuts at: 0 1280 1677 Size: 1280 397 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 237 1677 Size: 237 1440 EcoICRI GAG'CTC Cuts at: 0 226 1342 1677 Size: 226 1116 335 Fragments arranged by size: 1116 335 226 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 47 242 590 641 1223 1328 1422 1677 Size: 47 195 348 51 582 105 94 255 Fragments arranged by size: 582 348 255 195 105 94 51 47 EcoO109I rG'GnC_Cy Cuts at: 0 14 1677 Size: 14 1663 EcoRI G'AATT_C Cuts at: 0 1327 1677 Size: 1327 350 EcoRV GAT'ATC Cuts at: 0 961 1677 Size: 961 716 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 595 900 1058 1677 Size: 595 305 158 619 Fragments arranged by size: 619 595 305 158 HaeII r_GCGC'y Cuts at: 0 176 362 509 1677 Size: 176 186 147 1168 Fragments arranged by size: 1168 186 176 147 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 317 349 577 609 1551 1583 1677 Size: 317 32 228 32 942 32 94 Fragments arranged by size: 942 317 228 94 32 32 32 HincII GTy'rAC Cuts at: 0 917 1524 1677 Size: 917 607 153 Fragments arranged by size: 917 607 153 HpaI GTT'AAC Cuts at: 0 917 1677 Size: 917 760 KasI G'GCGC_C Cuts at: 0 172 505 1677 Size: 172 333 1172 Fragments arranged by size: 1172 333 172 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1636 1677 Size: 1636 41 MslI CAynn'nnrTG Cuts at: 0 907 1112 1475 1583 1677 Size: 907 205 363 108 94 Fragments arranged by size: 907 363 205 108 94 MspA1I CmG'CkG Cuts at: 0 401 472 1251 1677 Size: 401 71 779 426 Fragments arranged by size: 779 426 401 71 NaeI GCC'GGC Cuts at: 0 286 1677 Size: 286 1391 NarI GG'CG_CC Cuts at: 0 173 506 1677 Size: 173 333 1171 Fragments arranged by size: 1171 333 173 NgoMIV G'CCGG_C Cuts at: 0 284 1677 Size: 284 1393 NspI r_CATG'y Cuts at: 0 1143 1677 Size: 1143 534 PciI A'CATG_T Cuts at: 0 1139 1677 Size: 1139 538 PspOMI G'GGCC_C Cuts at: 0 13 1677 Size: 13 1664 PspXI vC'TCGA_Gb Cuts at: 0 520 1677 Size: 520 1157 PstI C_TGCA'G Cuts at: 0 307 1677 Size: 307 1370 PvuII CAG'CTG Cuts at: 0 1251 1677 Size: 1251 426 SacI G_AGCT'C Cuts at: 0 228 1344 1677 Size: 228 1116 333 Fragments arranged by size: 1116 333 228 SacII CC_GC'GG Cuts at: 0 402 473 1677 Size: 402 71 1204 Fragments arranged by size: 1204 402 71 ScaI AGT'ACT Cuts at: 0 526 1677 Size: 526 1151 SfcI C'TryA_G Cuts at: 0 303 1677 Size: 303 1374 SfiI GGCCn_nnn'nGGCC Cuts at: 0 329 350 1677 Size: 329 21 1327 Fragments arranged by size: 1327 329 21 SfoI GGC'GCC Cuts at: 0 174 507 1677 Size: 174 333 1170 Fragments arranged by size: 1170 333 174 SmaI CCC'GGG Cuts at: 0 120 254 294 1677 Size: 120 134 40 1383 Fragments arranged by size: 1383 134 120 40 SmlI C'TyrA_G Cuts at: 0 520 1287 1677 Size: 520 767 390 Fragments arranged by size: 767 520 390 SrfI GCCC'GGGC Cuts at: 0 294 1677 Size: 294 1383 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 514 753 854 1271 1677 Size: 514 239 101 417 406 Fragments arranged by size: 514 417 406 239 101 TatI w'GTAC_w Cuts at: 0 524 1312 1571 1677 Size: 524 788 259 106 Fragments arranged by size: 788 524 259 106 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1331 1363 1677 Size: 1331 32 314 Fragments arranged by size: 1331 314 32 XhoI C'TCGA_G Cuts at: 0 520 1677 Size: 520 1157 XmaI C'CCGG_G Cuts at: 0 118 252 292 1677 Size: 118 134 40 1385 Fragments arranged by size: 1385 134 118 40 XmnI GAAnn'nnTTC Cuts at: 0 1041 1458 1677 Size: 1041 417 219 Fragments arranged by size: 1041 417 219 Enzymes that do cut: AccI AcuI AflIII ApaI ApoI AvaI BanI BanII BbeI BbsI BbvCI BglI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrFI BssSI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HpaI KasI MmeI MslI MspA1I NaeI NarI NgoMIV NspI PciI PspOMI PspXI PstI PvuII SacI SacII ScaI SfcI SfiI SfoI SmaI SmlI SrfI TaqII TatI TstI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI AlwNI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BcgI BciVI BclI BfrBI BglII BlpI BmrI BmtI BplI BsaI BsaAI BsaBI BsiWI BsmI BsmBI BsrBI BsrDI BsrGI BssHII BstAPI BstBI BstEII ClaI CspCI DraI DraIII DrdI EcoNI FseI FspI FspAI HindIII KpnI MfeI MluI MscI NcoI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PvuI RsrII SalI SanDI SapI SbfI SexAI SgfI SgrAI SnaBI SpeI SphI SspI StuI StyI SwaI Tth111I XbaI XcmI ZraI