(Linear) (Six-base) MAPSORT of: KIEE3291.seq Check: 702 from: 1 to: 2259 KIEE3291.orf With 182 enzymes: SgfI * March 22, 2012 16:29 .. AatII G_ACGT'C Cuts at: 0 2138 2259 Size: 2138 121 AccI GT'mk_AC Cuts at: 0 1393 1634 2259 Size: 1393 241 625 Fragments arranged by size: 1393 625 241 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 418 1123 2259 Size: 418 705 1136 Fragments arranged by size: 1136 705 418 AflIII A'CryG_T Cuts at: 0 933 1051 2259 Size: 933 118 1208 Fragments arranged by size: 1208 933 118 AleI CACnn'nnGTG Cuts at: 0 316 2259 Size: 316 1943 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1175 1207 2259 Size: 1175 32 1052 Fragments arranged by size: 1175 1052 32 AlwNI CAG_nnn'CTG Cuts at: 0 594 998 1471 2259 Size: 594 404 473 788 Fragments arranged by size: 788 594 473 404 ApoI r'AATT_y Cuts at: 0 1249 1490 1672 2259 Size: 1249 241 182 587 Fragments arranged by size: 1249 587 241 182 AseI AT'TA_AT Cuts at: 0 1430 2259 Size: 1430 829 AvaI C'yCGr_G Cuts at: 0 1532 2259 Size: 1532 727 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1710 1744 2259 Size: 1710 34 515 Fragments arranged by size: 1710 515 34 BclI T'GATC_A Cuts at: 0 1037 2259 Size: 1037 1222 BmgBI CAC'GTC Cuts at: 0 936 1054 2172 2259 Size: 936 118 1118 87 Fragments arranged by size: 1118 936 118 87 BmrI ACTGGGnnnn_n' Cuts at: 0 1801 2259 Size: 1801 458 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1296 1596 2259 Size: 1296 300 663 Fragments arranged by size: 1296 663 300 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1268 2259 Size: 1268 991 BsaBI GATnn'nnATC Cuts at: 0 472 2259 Size: 472 1787 BsaHI Gr'CG_yC Cuts at: 0 2135 2259 Size: 2135 124 BsaWI w'CCGG_w Cuts at: 0 2095 2259 Size: 2095 164 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1756 1786 2259 Size: 1756 30 473 Fragments arranged by size: 1756 473 30 BseYI C'CCAG_C Cuts at: 0 2211 2259 Size: 2211 48 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 170 555 602 944 1117 2185 2259 Size: 170 385 47 342 173 1068 74 Fragments arranged by size: 1068 385 342 173 170 74 47 BsmI GAATG_Cn' Cuts at: 0 774 1551 2259 Size: 774 777 708 Fragments arranged by size: 777 774 708 BspEI T'CCGG_A Cuts at: 0 2095 2259 Size: 2095 164 BspHI T'CATG_A Cuts at: 0 48 410 2259 Size: 48 362 1849 Fragments arranged by size: 1849 362 48 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1922 2259 Size: 1922 337 BsrDI GCAATG_nn' Cuts at: 0 2001 2259 Size: 2001 258 BstAPI GCAn_nnn'nTGC Cuts at: 0 1131 2259 Size: 1131 1128 BstYI r'GATC_y Cuts at: 0 2252 2259 Size: 2252 7 BstZ17I GTA'TAC Cuts at: 0 1394 2259 Size: 1394 865 Bsu36I CC'TnA_GG Cuts at: 0 1591 2259 Size: 1591 668 BtgI C'CryG_G Cuts at: 0 341 1764 1842 2259 Size: 341 1423 78 417 Fragments arranged by size: 1423 417 341 78 BtsI GCAGTG_nn' Cuts at: 0 585 1129 1237 2259 Size: 585 544 108 1022 Fragments arranged by size: 1022 585 544 108 ClaI AT'CG_AT Cuts at: 0 1223 2259 Size: 1223 1036 DraIII CAC_nnn'GTG Cuts at: 0 756 2259 Size: 756 1503 EaeI y'GGCC_r Cuts at: 0 449 1322 1518 1677 2105 2259 Size: 449 873 196 159 428 154 Fragments arranged by size: 873 449 428 196 159 154 EarI CTCTTCn'nnn_ Cuts at: 0 1059 1415 2259 Size: 1059 356 844 Fragments arranged by size: 1059 844 356 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1678 2259 Size: 1678 581 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 418 1123 2259 Size: 418 705 1136 Fragments arranged by size: 1136 705 418 EcoNI CCTnn'n_nnAGG Cuts at: 0 1535 1881 2259 Size: 1535 346 378 Fragments arranged by size: 1535 378 346 EcoO109I rG'GnC_Cy Cuts at: 0 43 1595 1911 2259 Size: 43 1552 316 348 Fragments arranged by size: 1552 348 316 43 EcoRV GAT'ATC Cuts at: 0 408 599 2259 Size: 408 191 1660 Fragments arranged by size: 1660 408 191 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 706 738 2259 Size: 706 32 1521 Fragments arranged by size: 1521 706 32 HincII GTy'rAC Cuts at: 0 2199 2259 Size: 2199 60 MfeI C'AATT_G Cuts at: 0 314 2259 Size: 314 1945 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 996 2259 Size: 996 1263 MscI TGG'CCA Cuts at: 0 451 1679 2107 2259 Size: 451 1228 428 152 Fragments arranged by size: 1228 451 428 152 MslI CAynn'nnrTG Cuts at: 0 316 560 697 784 1751 2259 Size: 316 244 137 87 967 508 Fragments arranged by size: 967 508 316 244 137 87 MspA1I CmG'CkG Cuts at: 0 998 2026 2259 Size: 998 1028 233 Fragments arranged by size: 1028 998 233 NcoI C'CATG_G Cuts at: 0 341 1842 2259 Size: 341 1501 417 Fragments arranged by size: 1501 417 341 NspI r_CATG'y Cuts at: 0 538 702 2259 Size: 538 164 1557 Fragments arranged by size: 1557 538 164 PfoI T'CCnGG_A Cuts at: 0 1094 2259 Size: 1094 1165 PpuMI rG'GwC_Cy Cuts at: 0 43 1595 1911 2259 Size: 43 1552 316 348 Fragments arranged by size: 1552 348 316 43 PshAI GACnn'nnGTC Cuts at: 0 835 2259 Size: 835 1424 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 664 696 1384 1416 2259 Size: 664 32 688 32 843 Fragments arranged by size: 843 688 664 32 32 PstI C_TGCA'G Cuts at: 0 23 1464 1489 1788 2259 Size: 23 1441 25 299 471 Fragments arranged by size: 1441 471 299 25 23 PvuII CAG'CTG Cuts at: 0 998 2026 2259 Size: 998 1028 233 Fragments arranged by size: 1028 998 233 SfcI C'TryA_G Cuts at: 0 19 745 1441 1460 1485 1784 2259 Size: 19 726 696 19 25 299 475 Fragments arranged by size: 726 696 475 299 25 19 19 SmlI C'TyrA_G Cuts at: 0 1283 2259 Size: 1283 976 SspI AAT'ATT Cuts at: 0 852 1121 2259 Size: 852 269 1138 Fragments arranged by size: 1138 852 269 StuI AGG'CCT Cuts at: 0 359 2152 2259 Size: 359 1793 107 Fragments arranged by size: 1793 359 107 StyI C'CwwG_G Cuts at: 0 341 1195 1319 1680 1842 2259 Size: 341 854 124 361 162 417 Fragments arranged by size: 854 417 361 341 162 124 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1123 1835 1948 2203 2259 Size: 1123 712 113 255 56 Fragments arranged by size: 1123 712 255 113 56 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 583 615 1019 1051 1756 1788 2259 Size: 583 32 404 32 705 32 471 Fragments arranged by size: 705 583 471 404 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 546 2229 2259 Size: 546 1683 30 Fragments arranged by size: 1683 546 30 ZraI GAC'GTC Cuts at: 0 2136 2259 Size: 2136 123 Enzymes that do cut: AatII AccI AcuI AflIII AleI AloI AlwNI ApoI AseI AvaI BcgI BclI BmgBI BmrI BplI BpuEI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsmI BspEI BspHI BspMI BsrDI BstAPI BstYI BstZ17I Bsu36I BtgI BtsI ClaI DraIII EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRV Hin4I HincII MfeI MmeI MscI MslI MspA1I NcoI NspI PfoI PpuMI PshAI PsrI PstI PvuII SfcI SmlI SspI StuI StyI TaqII TstI XcmI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AflII AgeI AhdI AlfI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI BfrBI BglI BglII BlpI Bme1580I BmtI BpmI Bpu10I BsaI BsaAI BseRI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BtgZI CspCI DraI DrdI EagI EcoICRI EcoRI FalI FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PsiI PspOMI PspXI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI TatI Tth111I XbaI XhoI XmaI XmnI