Restriction map for
pFN21AE2526
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BamHI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaBI
BsaI BsaWI BseAI BsiWI BsmFI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BsrI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DdeI DraI DraIII
DrdI Eam1105I Eco47III Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HinfI HpaI KpnI MaeI MaeIII
MamI MfeI MflI MluI MroI
MseI MunI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PleI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI ScaI SexAI SfaNI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tru9I
Tsp45I Tsp509I TthHB8I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AspHI 544 GAGCTC G[AT]GC[AT]^C
AspI 469 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 564 TGGCCA TGG^CCA
BbeI 271 GGCGCC GGCGC^C
BbrPI 214 CACGTG CAC^GTG
BcnI 429 CCGGG CC^[GC]GG
BsaAI 214 CACGTG [CT]AC^GT[GA]
BseRI 229 GAGGAG GAGGAG(10/8)
BsiEI 426 CGGCCG CG[GA][CT]^CG
BsiHKAI 544 GAGCTC G[AT]GC[AT]^C
BsmAI 355 GAGAC GTCTC(1/5)
BsmBI 355 GAGACG CGTCTC(1/5)
BsmI 252 GAATGC GAATGC(1/-1)
BsrFI 17 GCCGGC [GA]^CCGG[CT]
BssSI 160 CACGAG CACGAG(-5/-1)
BstXI 403 CCAAACACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 17 GCCGGC [GA]^CCGG[CT]
DpnII 183 GATC ^GATC
EagI 426 CGGCCG C^GGCCG
EclXI 426 CGGCCG C^GGCCG
Eco52I 426 CGGCCG C^GGCCG
EcoT14I 329 CCTTGG C^C[AT][AT]GG
HaeII 271 GGCGCC [GA]GCGC^[CT]
HgaI 434 GCGTC GACGC(5/10)
KasI 271 GGCGCC G^GCGCC
KspI 339 CCGCGG CCGC^GG
MaeII 215 ACGT A^CGT
MboI 183 GATC ^GATC
MluNI 564 TGGCCA TGG^CCA
MscI 564 TGGCCA TGG^CCA
MslI 408 CACATGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 17 GCCGGC GCC^GGC
NarI 271 GGCGCC GG^CGCC
NciI 429 CCGGG CC^[GC]GG
NdeII 183 GATC ^GATC
NgoMIV 17 GCCGGC G^CCGGC
PflFI 469 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 159 CCACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 214 CACGTG CAC^GTG
PmlI 214 CACGTG CAC^GTG
PpuMI 569 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 66 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 235 CTGCAG CTGCA^G
PvuII 386 CAGCTG CAG^CTG
SacI 544 GAGCTC GAGCT^C
SacII 339 CCGCGG CCGC^GG
SapI 315 GCTCTTC GCTCTTC(1/4)
Sau3AI 183 GATC ^GATC
SfcI 235 CTGCAG C^T[GA][CT]AG
SfoI 271 GGCGCC GGC^GCC
SmlI 541 CTTGAG C^T[CT][GA]AG
StyI 329 CCTTGG C^C[AT][AT]GG
Tth111I 469 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 159 CCACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcyI 271 GGCGCC G[GA]^CG[CT]C
433 GGCGTC
AvaI 348 CTCGGG C^[CT]CG[GA]G
527 CTCGGG
AvaII 156 GGACC G^G[AT]CC
570 GGACC
BanI 271 GGCGCC G^G[CT][GA]CC
322 GGCACC
BanII 333 GGGCTC G[GA]GC[CT]^C
544 GAGCTC
BbsI 106 GTCTTC GAAGAC(2/6)
475 GTCTTC
BmyI 333 GGGCTC G[AGT]GC[ACT]^C
544 GAGCTC
BpuAI 106 GTCTTC GAAGAC(2/6)
475 GTCTTC
BsaHI 271 GGCGCC G[GA]^CG[CT]C
433 GGCGTC
BsgI 202 CTGCAC GTGCAG(16/14)
286 CTGCAC
BsoBI 348 CTCGGG C^[CT]CG[GA]G
527 CTCGGG
Bsp1286I 333 GGGCTC G[AGT]GC[ACT]^C
544 GAGCTC
DraII 530 GGGGCCT [GA]G^G[ACGT]CC[CT]
569 AGGACCC
DsaI 11 CCGTGG C^C[GA][CT]GG
339 CCGCGG
EcoO109I 530 GGGGCCT [GA]G^G[ACGT]CC[CT]
569 AGGACCC
FauI 338 CCCGC CCCGC(4/6)
256 GCGGG
Hin1I 271 GGCGCC G[GA]^CG[CT]C
433 GGCGTC
NlaIII 276 CATG CATG^
410 CATG
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AccII AciI AfaI AluI BbvI
BsaJI BsiYI BslI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
EaeI EarI EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI Ksp632I MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NlaIV RsaI Sau96I ScrFI TseI
TspRI