(Linear) (Six-base) MAPSORT of: KIEE2526.seq Check: 5469 from: 1 to: 576 KIEE2526.orf With 182 enzymes: SgfI * June 11, 2014 14:42 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 462 576 Size: 462 114 AvaI C'yCGr_G Cuts at: 0 348 527 576 Size: 348 179 49 Fragments arranged by size: 348 179 49 BanI G'GyrC_C Cuts at: 0 271 322 576 Size: 271 51 254 Fragments arranged by size: 271 254 51 BanII G_rGCy'C Cuts at: 0 337 548 576 Size: 337 211 28 Fragments arranged by size: 337 211 28 BbeI G_GCGC'C Cuts at: 0 275 576 Size: 275 301 BbsI GAAGACnn'nnnn_ Cuts at: 0 99 468 576 Size: 99 369 108 Fragments arranged by size: 369 108 99 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 562 576 Size: 562 14 BsaAI yAC'GTr Cuts at: 0 216 576 Size: 216 360 BsaHI Gr'CG_yC Cuts at: 0 272 434 576 Size: 272 162 142 Fragments arranged by size: 272 162 142 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 223 253 576 Size: 223 30 323 Fragments arranged by size: 323 223 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 244 576 Size: 244 332 BseYI C'CCAG_C Cuts at: 0 87 148 576 Size: 87 61 428 Fragments arranged by size: 428 87 61 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 187 271 576 Size: 187 84 305 Fragments arranged by size: 305 187 84 BsiEI CG_ry'CG Cuts at: 0 429 576 Size: 429 147 BsiHKAI G_wGCw'C Cuts at: 0 548 576 Size: 548 28 BsmI GAATG_Cn' Cuts at: 0 258 576 Size: 258 318 BsmBI CGTCTCn'nnnn_ Cuts at: 0 349 576 Size: 349 227 Bsp1286I G_dGCh'C Cuts at: 0 337 548 576 Size: 337 211 28 Fragments arranged by size: 337 211 28 BsrFI r'CCGG_y Cuts at: 0 17 576 Size: 17 559 BssSI C'ACGA_G Cuts at: 0 160 576 Size: 160 416 BstXI CCAn_nnnn'nTGG Cuts at: 0 410 576 Size: 410 166 BtgI C'CryG_G Cuts at: 0 11 339 576 Size: 11 328 237 Fragments arranged by size: 328 237 11 BtsI GCAGTG_nn' Cuts at: 0 10 244 576 Size: 10 234 332 Fragments arranged by size: 332 234 10 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 371 406 576 Size: 371 35 170 Fragments arranged by size: 371 170 35 EaeI y'GGCC_r Cuts at: 0 342 426 464 564 576 Size: 342 84 38 100 12 Fragments arranged by size: 342 100 84 38 12 EagI C'GGCC_G Cuts at: 0 426 576 Size: 426 150 EarI CTCTTCn'nnn_ Cuts at: 0 113 322 508 518 576 Size: 113 209 186 10 58 Fragments arranged by size: 209 186 113 58 10 EcoICRI GAG'CTC Cuts at: 0 546 576 Size: 546 30 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 462 576 Size: 462 114 EcoO109I rG'GnC_Cy Cuts at: 0 531 570 576 Size: 531 39 6 Fragments arranged by size: 531 39 6 HaeII r_GCGC'y Cuts at: 0 275 576 Size: 275 301 KasI G'GCGC_C Cuts at: 0 271 576 Size: 271 305 MscI TGG'CCA Cuts at: 0 566 576 Size: 566 10 MslI CAynn'nnrTG Cuts at: 0 412 576 Size: 412 164 MspA1I CmG'CkG Cuts at: 0 58 341 388 576 Size: 58 283 47 188 Fragments arranged by size: 283 188 58 47 NaeI GCC'GGC Cuts at: 0 19 576 Size: 19 557 NarI GG'CG_CC Cuts at: 0 272 576 Size: 272 304 NgoMIV G'CCGG_C Cuts at: 0 17 576 Size: 17 559 PflMI CCAn_nnn'nTGG Cuts at: 0 165 576 Size: 165 411 PmlI CAC'GTG Cuts at: 0 216 576 Size: 216 360 PpuMI rG'GwC_Cy Cuts at: 0 570 576 Size: 570 6 PshAI GACnn'nnGTC Cuts at: 0 70 576 Size: 70 506 PstI C_TGCA'G Cuts at: 0 239 576 Size: 239 337 PvuII CAG'CTG Cuts at: 0 388 576 Size: 388 188 SacI G_AGCT'C Cuts at: 0 548 576 Size: 548 28 SacII CC_GC'GG Cuts at: 0 342 576 Size: 342 234 SapI GCTCTTCn'nnn_ Cuts at: 0 322 576 Size: 322 254 SfcI C'TryA_G Cuts at: 0 235 576 Size: 235 341 SfoI GGC'GCC Cuts at: 0 273 576 Size: 273 303 SmlI C'TyrA_G Cuts at: 0 541 576 Size: 541 35 StyI C'CwwG_G Cuts at: 0 329 576 Size: 329 247 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 221 231 499 576 Size: 221 10 268 77 Fragments arranged by size: 268 221 77 10 TatI w'GTAC_w Cuts at: 0 363 576 Size: 363 213 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 223 255 576 Size: 223 32 321 Fragments arranged by size: 321 223 32 Tth111I GACn'n_nGTC Cuts at: 0 472 576 Size: 472 104 Enzymes that do cut: AcuI AvaI BanI BanII BbeI BbsI BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BsrFI BssSI BstXI BtgI BtsI CspCI EaeI EagI EarI EcoICRI Eco57MI EcoO109I HaeII KasI MscI MslI MspA1I NaeI NarI NgoMIV PflMI PmlI PpuMI PshAI PstI PvuII SacI SacII SapI SfcI SfoI SmlI StyI TaqII TatI TstI Tth111I Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BsaI BsaBI BsaWI BsiWI BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI NcoI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PfoI PmeI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI XbaI XcmI XhoI XmaI XmnI ZraI