################################## # NO CUT ENZYMES ( 166 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcyI AfaI AflII AflIII AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I FauI FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MfeI MflI MluI MspA1I MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NspI NspV PacI PflFI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SspI StuI StyI SwaI Tsp45I Tth111I XbaI XcmI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccIII 83 TCCGGA T^CCGGA AcsI 187 AAATTT [GA]^AATT[CT] AgeI 69 ACCGGT A^CCGGT AlwI 86 GGATC GGATC(4/5) ApoI 187 AAATTT [GA]^AATT[CT] AvaI 43 CTCGAG C^[CT]CG[GA]G BalI 128 TGGCCA TGG^CCA BanI 245 GGCACC G^G[CT][GA]CC BanII 397 GAGCCC G[GA]GC[CT]^C BbvI 9 GCAGC GCAGC(8/12) BglI 511 GCCACTAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 397 GAGCCC G[AGT]GC[ACT]^C BseAI 83 TCCGGA T^CCGGA BsmAI 455 GAGAC GTCTC(1/5) BsmFI 547 GTCCC GGGAC(10/14) BsoBI 43 CTCGAG C^[CT]CG[GA]G Bsp1286I 397 GAGCCC G[AGT]GC[ACT]^C BspEI 83 TCCGGA T^CCGGA BsrFI 69 ACCGGT [GA]^CCGG[CT] BsrI 119 ACTGG ACTGG(1/-1) BstXI 551 CCACAACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 23 GCTCGC GC[ACGT]^[ACGT]GC CfoI 178 GCGC GCG^C Cfr10I 69 ACCGGT [GA]^CCGG[CT] DpnII 87 GATC ^GATC DraIII 311 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 128 TGGCCA [CT]^GGCC[GA] EcoNI 57 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 276 ATGCAT ATGCA^T HhaI 178 GCGC GCG^C HinP1I 178 GCGC G^CGC MboI 87 GATC ^GATC MluNI 128 TGGCCA TGG^CCA MroI 83 TCCGGA T^CCGGA MscI 128 TGGCCA TGG^CCA MslI 90 CACCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NdeII 87 GATC ^GATC NsiI 276 ATGCAT ATGCA^T PaeR7I 43 CTCGAG C^TCGAG PinAI 69 ACCGGT A^CCGGT PpuMI 545 AGGTCCC [GA]G^G[AT]CC[CT] PstI 58 CTGCAG CTGCA^G Sau3AI 87 GATC ^GATC SbfI 57 CCTGCAGG CCTGCA^GG SmlI 43 CTCGAG C^T[CT][GA]AG Sse8387I 57 CCTGCAGG CCTGCA^GG TseI 9 GCAGC G^C[AT]GC TspRI 386 CACTG CA[GC]TG[ACGT][ACGT]^ XhoI 43 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AvaII 139 GGACC G^G[AT]CC 546 GGTCC BsaJI 92 CCCTGG C^C[ACGT][ACGT]GG 557 CCCTGG BsaWI 69 ACCGGT [AT]^CCGG[AT] 83 TCCGGA BssKI 93 CCTGG ^CC[ACGT]GG 558 CCTGG BstF5I 292 CATCC GGATG(2/0) 534 GGATG BstNI 93 CCTGG CC^[AT]GG 558 CCTGG DraII 32 AGGCCCT [GA]G^G[ACGT]CC[CT] 545 AGGTCCC EcoO109I 32 AGGCCCT [GA]G^G[ACGT]CC[CT] 545 AGGTCCC EcoRII 93 CCTGG ^CC[AT]GG 558 CCTGG Fnu4HI 9 GCAGC GC^[ACGT]GC 164 GCGGC FokI 292 CATCC GGATG(9/13) 534 GGATG HapII 70 CCGG C^CGG 84 CCGG HpaII 70 CCGG C^CGG 84 CCGG HphI 89 TCACC GGTGA(8/7) 211 TCACC ItaI 9 GCAGC GC^[ACGT]GC 164 GCGGC MspI 70 CCGG C^CGG 84 CCGG MvaI 93 CCTGG CC^[AT]GG 558 CCTGG MwoI 390 GCTTAATGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 511 GCCACTAAGGC PflMI 236 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 310 CCACAGAGTGG PleI 103 GAGTC GAGTC(4/5) 709 GAGTC ScrFI 93 CCTGG CC^[ACGT]GG 558 CCTGG SfcI 58 CTGCAG C^T[GA][CT]AG 299 CTGTAG Tsp509I 188 AATT ^AATT 489 AATT Van91I 236 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 310 CCACAGAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 19 enzymes) # ######################################### AciI AluI BseRI BsiYI BslI Cfr13I DdeI HaeIII HinfI MboII MnlI MseI NlaIV Sau96I SfaNI TaqI TfiI Tru9I TthHB8I