Restriction map for
pFN21AE3077
(Circular) (Six-base) MAPSORT of: pFN21AE3077.seq Check: 1989 from: 1 to: 5419
pFN21AE3077 5419 bp
With 182 enzymes: SgfI *
March 28, 2012 12:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3229 278
Size: 53 83 186 936 1693 2468
Fragments arranged by size:
2468 1693 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2762 1937
Size: 825 4594
Acc65I G'GTAC_C
Cuts at: 2740 2740
Size: 5419
AclI AA'CG_TT
Cuts at: 1563 3546 3919 1563
Size: 1983 373 3063
Fragments arranged by size:
3063 1983 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2212 2421 3482 4866 5134 1632
Size: 580 209 1061 1384 268 1917
Fragments arranged by size:
1917 1384 1061 580 268 209
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5222
AflIII A'CryG_T
Cuts at: 1203 4318 5295 1203
Size: 3115 977 1327
Fragments arranged by size:
3115 1327 977
AgeI A'CCGG_T
Cuts at: 2074 4250 2074
Size: 2176 3243
AhdI GACnn_n'nnGTC
Cuts at: 4148 4148
Size: 5419
AleI CACnn'nnGTG
Cuts at: 1166 2099 1166
Size: 933 4486
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5419
AlwNI CAG_nnn'CTG
Cuts at: 3162 4734 3162
Size: 1572 3847
ApaLI G'TGCA_C
Cuts at: 3476 4632 3476
Size: 1156 4263
ApoI r'AATT_y
Cuts at: 1493 2192 2728 2954 3106 5215 1493
Size: 699 536 226 152 2109 1697
Fragments arranged by size:
2109 1697 699 536 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3973 160
Size: 872 2941 1606
Fragments arranged by size:
2941 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5419
AvaI C'yCGr_G
Cuts at: 1870 1943 2048 2744 1870
Size: 73 105 696 4545
Fragments arranged by size:
4545 696 105 73
BamHI G'GATC_C
Cuts at: 2749 3116 2749
Size: 367 5052
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2250 2740 4195 618
Size: 325 217 627 463 490 1455 1842
Fragments arranged by size:
1842 1455 627 490 463 325 217
BanII G_rGCy'C
Cuts at: 1391 2406 2738 1391
Size: 1015 332 4072
Fragments arranged by size:
4072 1015 332
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4448
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3610 3644 1903
Size: 34 1673 34 3678
Fragments arranged by size:
3678 1673 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3311 4521 3311
Size: 1210 4209
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5419
BfrBI ATG'CAT
Cuts at: 2283 2283
Size: 5419
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2522 4030 136
Size: 107 122 71 631 605 850 1508 1525
Fragments arranged by size:
1525 1508 850 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5414 5414
Size: 5419
BlpI GC'TnA_GC
Cuts at: 2836 2836
Size: 5419
Bme1580I G_kGCm'C
Cuts at: 1790 3480 4636 1790
Size: 1690 1156 2573
Fragments arranged by size:
2573 1690 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5419
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2134 3150 4108
Size: 416 53 83 399 24 717 1016 958
Cuts at: 4108 442
Size: 1753
Fragments arranged by size:
1753 1016 958 717 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5419
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4079 1239
Size: 2840 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3544 4409 4707 3544
Size: 865 298 4256
Fragments arranged by size:
4256 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4082 882
Size: 617 2583 2219
Fragments arranged by size:
2583 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3210 493
Size: 711 2006 2702
Fragments arranged by size:
2702 2006 711
BsaBI GATnn'nnATC
Cuts at: 5413 5413
Size: 5419
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3226 3608 4243 275
Size: 53 83 186 936 1693 382 635 1451
Fragments arranged by size:
1693 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2074 2088 3852 4250 4524 4671 5082 2074
Size: 14 1764 398 274 147 411 2411
Fragments arranged by size:
2411 1764 411 398 274 147 14
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2399 2429 2449 2479 2535
Size: 30 165 30 996 30 20 30 56
Cuts at: 2535 2565 1178
Size: 30 4032
Fragments arranged by size:
4032 996 165 56 30 30 30 30
30 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2678 2681 2726 1186
Size: 1492 3 45 3879
Fragments arranged by size:
3879 1492 45 3
BseYI C'CCAG_C
Cuts at: 2010 2419 4622 5118 2010
Size: 409 2203 496 2311
Fragments arranged by size:
2311 2203 496 409
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4846
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2869 3630 4658 1540
Size: 275 186 868 761 1028 2301
Fragments arranged by size:
2301 1028 868 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3480 3565 4636 3480
Size: 85 1071 4263
Fragments arranged by size:
4263 1071 85
BsmI GAATG_Cn'
Cuts at: 2930 3023 2930
Size: 93 5326
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2406 2738 3480 3565 4636 1391
Size: 399 616 332 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 616 399 332 85
BspEI T'CCGG_A
Cuts at: 2088 5082 2088
Size: 2994 2425
BspHI T'CATG_A
Cuts at: 3308 5038 3308
Size: 1730 3689
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2774 844
Size: 1930 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3306 1394
Size: 429 1483 3507
Fragments arranged by size:
3507 1483 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3914 4088 65
Size: 1183 2666 174 1396
Fragments arranged by size:
2666 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2074 4063 4250 1700
Size: 110 264 1989 187 2869
Fragments arranged by size:
2869 1989 264 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5419
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5419
BssSI C'ACGA_G
Cuts at: 3479 4491 3479
Size: 1012 4407
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5419
BstBI TT'CG_AA
Cuts at: 4223 4223
Size: 5419
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5419
BstXI CCAn_nnnn'nTGG
Cuts at: 2563 2563
Size: 5419
BstYI r'GATC_y
Cuts at: 1972 2749 3116 3501 3518 4970 5056 5068 5414
Size: 777 367 385 17 1452 86 12 346
Cuts at: 5414 1972
Size: 1977
Fragments arranged by size:
1977 1452 777 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3928
Fragments arranged by size:
3928 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4680
Fragments arranged by size:
4680 683 56
BtsI GCAGTG_nn'
Cuts at: 2390 2935 3729 3749 2390
Size: 545 794 20 4060
Fragments arranged by size:
4060 794 545 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5419
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5384
DraI TTT'AAA
Cuts at: 2723 2723
Size: 5419
DraIII CAC_nnn'GTG
Cuts at: 2321 2321
Size: 5419
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4426 809
Size: 507 3110 1802
Fragments arranged by size:
3110 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2133 2866 3755
Size: 54 1425 50 275 18 303 733 889
Cuts at: 3755 5384 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 733 303 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2866 1537
Size: 275 1054 4090
Fragments arranged by size:
4090 1054 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3349 1353
Size: 1996 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2289 2582 4006 4380 4526 387
Size: 1064 138 700 293 1424 374 146 1280
Fragments arranged by size:
1424 1280 1064 700 374 293 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2212 2421 3482 4079 4866 5134 1239
Size: 393 580 209 1061 597 787 268 1524
Fragments arranged by size:
1524 1061 787 597 580 393 268 209
EcoNI CCTnn'n_nnAGG
Cuts at: 2066 2066
Size: 5419
EcoO109I rG'GnC_Cy
Cuts at: 2038 2551 2038
Size: 513 4906
EcoRI G'AATT_C
Cuts at: 2728 3106 2728
Size: 378 5041
FspI TGC'GCA
Cuts at: 3925 3925
Size: 5419
HaeII r_GCGC'y
Cuts at: 1678 4566 1678
Size: 2888 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2042 2074 2281 2313 2535
Size: 32 135 32 470 32 207 32 222
Cuts at: 2535 2567 2594 2626 4060 4092 4134 4166 1373
Size: 32 27 32 1434 32 42 32 2626
Fragments arranged by size:
2626 1434 470 222 207 135 42 32
32 32 32 32 32 32 32 27
HincII GTy'rAC
Cuts at: 669 1657 1938 2763 3009 669
Size: 988 281 825 246 3079
Fragments arranged by size:
3079 988 825 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5419
HpaI GTT'AAC
Cuts at: 3009 3009
Size: 5419
KpnI G_GTAC'C
Cuts at: 2744 2744
Size: 5419
MfeI C'AATT_G
Cuts at: 3018 3018
Size: 5419
MluI A'CGCG_T
Cuts at: 5295 5295
Size: 5419
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2136 4533 4717 1305
Size: 831 2397 184 2007
Fragments arranged by size:
2397 2007 831 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2135 10
Size: 54 1425 343 303 3294
Fragments arranged by size:
3294 1425 343 303 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2099 3377 3736 3895 518
Size: 648 933 1278 359 159 2042
Fragments arranged by size:
2042 1278 933 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2835 3512 4660 4905 1606
Size: 1229 677 1148 245 2120
Fragments arranged by size:
2120 1229 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3928
Fragments arranged by size:
3928 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5419
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5419
NotI GC'GGCC_GC
Cuts at: 2866 2866
Size: 5419
NsiI A_TGCA'T
Cuts at: 2285 2285
Size: 5419
NspI r_CATG'y
Cuts at: 1132 1229 2777 4322 1132
Size: 97 1548 1545 2229
Fragments arranged by size:
2229 1548 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5419
PciI A'CATG_T
Cuts at: 4318 4318
Size: 5419
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2247 2321 1108
Size: 1139 74 4206
Fragments arranged by size:
4206 1139 74
PmeI GTTT'AAAC
Cuts at: 2723 2723
Size: 5419
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3488 3520 5001 5033 3488
Size: 32 1481 32 3874
Fragments arranged by size:
3874 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2551 2551
Size: 5419
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5419
PsiI TTA'TAA
Cuts at: 2989 2989
Size: 5419
PspXI vC'TCGA_Gb
Cuts at: 2048 2048
Size: 5419
PstI C_TGCA'G
Cuts at: 830 2067 2771 830
Size: 1237 704 3478
Fragments arranged by size:
3478 1237 704
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5419
SalI G'TCGA_C
Cuts at: 1936 2761 1936
Size: 825 4594
SbfI CC_TGCA'GG
Cuts at: 2067 2771 2067
Size: 704 4715
ScaI AGT'ACT
Cuts at: 3667 5379 3667
Size: 1712 3707
SfcI C'TryA_G
Cuts at: 826 1045 2063 2304 2767 3902 4583 4774 826
Size: 219 1018 241 463 1135 681 191 1471
Fragments arranged by size:
1471 1135 1018 681 463 241 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5419
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5419
SmaI CCC'GGG
Cuts at: 1872 2746 1872
Size: 874 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2048 3523 4424 4686 820
Size: 197 926 105 1475 901 262 1553
Fragments arranged by size:
1553 1475 926 901 262 197 105
SnaBI TAC'GTA
Cuts at: 493 3210 493
Size: 2717 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5419
SphI G_CATG'C
Cuts at: 1132 2777 1132
Size: 1645 3774
SspI AAT'ATT
Cuts at: 5 52 3343 5
Size: 47 3291 2081
Fragments arranged by size:
3291 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5419
StyI C'CwwG_G
Cuts at: 513 1065 2004 2858 513
Size: 552 939 854 3074
Fragments arranged by size:
3074 939 854 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3446 3463 3616 3801 4246 325
Size: 1255 1866 17 153 185 445 1498
Fragments arranged by size:
1866 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3665 5377 96
Size: 274 80 33 51 1443 1688 1712 138
Fragments arranged by size:
1712 1688 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5379 5411 5379
Size: 32 5387
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5419
XbaI T'CTAG_A
Cuts at: 2755 2755
Size: 5419
XhoI C'TCGA_G
Cuts at: 1943 2048 1943
Size: 105 5314
XmaI C'CCGG_G
Cuts at: 1870 2744 1870
Size: 874 4545
XmnI GAAnn'nnTTC
Cuts at: 3548 3548
Size: 5419
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3227 276
Size: 53 83 186 936 1693 2468
Fragments arranged by size:
2468 1693 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspXI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PspOMI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI