Restriction map for
pFN21AE1784
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# NO CUT ENZYMES ( 148 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BbeI BbrPI BbvCI BciVI
BfaI BfrI BglII BlnI BsaAI
BsaBI BsaHI BsaWI BseAI BseRI
BsgI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstYI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
Hin1I HindIII HinfI HpaI KasI
KpnI KspI MaeI MaeII MamI
MfeI MflI MluI MroI MseI
MunI MvnI NarI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tsp509I TspRI Tth111I Van91I XbaI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 5 GTCGAC GT^[AC][GT]AC
AfaI 780 GTAC GT^AC
AlwI 293 GGATC GGATC(4/5)
AspHI 139 GTGCTC G[AT]GC[AT]^C
BcgI 209 GCATCCTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 470 TGATCA T^GATCA
BglI 416 GCCCCAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 141 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 141 GCTCAGC GC^T[ACGT]AGC
BsaI 228 GGTCTC GGTCTC(1/5)
BsiEI 710 CGACCG CG[GA][CT]^CG
BsiHKAI 139 GTGCTC G[AT]GC[AT]^C
BsmAI 229 GTCTC GTCTC(1/5)
BsrDI 762 CATTGC GCAATG(2/0)
BsrI 489 CCAGT ACTGG(1/-1)
BssSI 281 CTCGTG CACGAG(-5/-1)
CelII 141 GCTCAGC GC^T[ACGT]AGC
CfoI 73 GCGC GCG^C
DdeI 142 CTCAG C^T[ACGT]AG
EarI 832 GAAGAG CTCTTC(1/4)
EcoT14I 614 CCATGG C^C[AT][AT]GG
FbaI 470 TGATCA T^GATCA
HaeII 72 GGCGCT [GA]GCGC^[CT]
HgaI 324 GACGC GACGC(5/10)
HhaI 73 GCGC GCG^C
HinP1I 73 GCGC G^CGC
Ksp632I 832 GAAGAG CTCTTC(1/4)
NaeI 430 GCCGGC GCC^GGC
NcoI 614 CCATGG C^CATGG
NgoMIV 430 GCCGGC G^CCGGC
PpuMI 91 GGGACCC [GA]G^G[AT]CC[CT]
PstI 698 CTGCAG CTGCA^G
PvuII 784 CAGCTG CAG^CTG
RsaI 780 GTAC GT^AC
SalI 5 GTCGAC G^TCGAC
SfcI 698 CTGCAG C^T[GA][CT]AG
SmaI 650 CCCGGG CCC^GGG
StyI 614 CCATGG C^C[AT][AT]GG
TaqI 6 TCGA T^CGA
TthHB8I 6 TCGA T^CGA
XmaCI 650 CCCGGG C^CCGGG
XmaI 650 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AlwNI 70 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
322 CAGACGCTG
ApaI 236 GGGCCC GGGCC^C
414 GGGCCC
AvaI 560 CCCGAG C^[CT]CG[GA]G
650 CCCGGG
AvaII 92 GGACC G^G[AT]CC
594 GGTCC
BalI 222 TGGCCA TGG^CCA
527 TGGCCA
BbsI 703 GTCTTC GAAGAC(2/6)
772 GTCTTC
BpmI 568 CTGGAG CTGGAG(16/14)
805 CTGGAG
BpuAI 703 GTCTTC GAAGAC(2/6)
772 GTCTTC
BsoBI 560 CCCGAG C^[CT]CG[GA]G
650 CCCGGG
BstXI 173 CCATCAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
225 CCAGGTCTCTGG
MaeIII 199 GTCAC ^GT[ACGT]AC
256 GTGAC
MluNI 222 TGGCCA TGG^CCA
527 TGGCCA
MscI 222 TGGCCA TGG^CCA
527 TGGCCA
MslI 99 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
609 CACCTCCATG
MspA1I 27 CCGCTG C[AC]G^C[GT]G
784 CAGCTG
StuI 564 AGGCCT AGG^CCT
572 AGGCCT
Tsp45I 199 GTCAC ^GT[GC]AC
256 GTGAC
XcmI 266 CCATGTGCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
305 CCATCTACACCCTGG
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AluI BanI BanII BbvI
BcnI BmyI BsaJI BsiYI BslI
BsmFI Bsp1286I BsrFI BssKI BstF5I
BstNI Cac8I Cfr10I Cfr13I DpnII
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HincII HindII HpaII HphI ItaI
MboI MboII MnlI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfaNI TseI