Restriction map for
pFN21AE1784
(Circular) (Six-base) MAPSORT of: pFN21AE1784.seq Check: 2020 from: 1 to: 5581
pFN21AE1784 5581 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3391 278
Size: 53 83 186 936 1855 2468
Fragments arranged by size:
2468 1855 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2011 2924 1937
Size: 74 913 4594
Fragments arranged by size:
4594 913 74
Acc65I G'GTAC_C
Cuts at: 2902 2902
Size: 5581
AclI AA'CG_TT
Cuts at: 1563 3708 4081 1563
Size: 2145 373 3063
Fragments arranged by size:
3063 2145 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3644 5028 5296 1632
Size: 2012 1384 268 1917
Fragments arranged by size:
2012 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5384
AflIII A'CryG_T
Cuts at: 1203 4480 5457 1203
Size: 3277 977 1327
Fragments arranged by size:
3277 1327 977
AgeI A'CCGG_T
Cuts at: 4412 4412
Size: 5581
AhdI GACnn_n'nnGTC
Cuts at: 4310 4310
Size: 5581
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5581
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5581
AlwNI CAG_nnn'CTG
Cuts at: 2080 2332 3324 4896 2080
Size: 252 992 1572 2765
Fragments arranged by size:
2765 1572 992 252
ApaI G_GGCC'C
Cuts at: 2245 2423 2245
Size: 178 5403
ApaLI G'TGCA_C
Cuts at: 3638 4794 3638
Size: 1156 4425
ApoI r'AATT_y
Cuts at: 1493 2890 3116 3268 5377 1493
Size: 1397 226 152 2109 1697
Fragments arranged by size:
2109 1697 1397 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4135 160
Size: 872 3103 1606
Fragments arranged by size:
3103 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5581
AvaI C'yCGr_G
Cuts at: 1870 1943 2565 2655 2906 1870
Size: 73 622 90 251 4545
Fragments arranged by size:
4545 622 251 90 73
BamHI G'GATC_C
Cuts at: 2911 3278 2911
Size: 367 5214
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2156 2612 2737 2902 4357
Size: 325 217 627 369 456 125 165 1455
Cuts at: 4357 618
Size: 1842
Fragments arranged by size:
1842 1455 627 456 369 325 217 165
125
BanII G_rGCy'C
Cuts at: 1391 2245 2295 2423 2702 2765 2900 1391
Size: 854 50 128 279 63 135 4072
Fragments arranged by size:
4072 854 279 135 128 63 50
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2701 2770 928
Size: 971 802 69 3739
Fragments arranged by size:
3739 971 802 69
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2203 2237 3772 3806 1903
Size: 34 266 34 1535 34 3678
Fragments arranged by size:
3678 1535 266 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3473 4683 3473
Size: 1210 4371
BclI T'GATC_A
Cuts at: 1548 2475 1548
Size: 927 4654
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2427 4192 136
Size: 107 122 71 631 605 755 1765 1525
Fragments arranged by size:
1765 1525 755 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5576 5576
Size: 5581
BlpI GC'TnA_GC
Cuts at: 2147 2998 2147
Size: 851 4730
Bme1580I G_kGCm'C
Cuts at: 1790 2159 2245 2423 2742 3642 4798 1790
Size: 369 86 178 319 900 1156 2573
Fragments arranged by size:
2573 1156 900 369 319 178 86
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5581
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3312 4270 442
Size: 416 53 83 399 24 1895 958 1753
Fragments arranged by size:
1895 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5581
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2594 2831 4241 1239
Size: 1355 237 1410 2579
Fragments arranged by size:
2579 1410 1355 237
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3706 4571 4869 3706
Size: 865 298 4418
Fragments arranged by size:
4418 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2239 4244 882
Size: 617 740 2005 2219
Fragments arranged by size:
2219 2005 740 617
BsaAI yAC'GTr
Cuts at: 493 1204 3372 493
Size: 711 2168 2702
Fragments arranged by size:
2702 2168 711
BsaBI GATnn'nnATC
Cuts at: 5575 5575
Size: 5581
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3388 3770 4405 275
Size: 53 83 186 936 1855 382 635 1451
Fragments arranged by size:
1855 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4014 4412 4686 4833 5244 4014
Size: 398 274 147 411 4351
Fragments arranged by size:
4351 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2012 2042 1178
Size: 30 165 30 609 30 4717
Fragments arranged by size:
4717 609 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5581
BseYI C'CCAG_C
Cuts at: 2332 2694 4784 5280 2332
Size: 362 2090 496 2633
Fragments arranged by size:
2633 2090 496 362
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5008
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2718 3031 3792 4820 1540
Size: 275 186 717 313 761 1028 2301
Fragments arranged by size:
2301 1028 761 717 313 275 186
BsiHKAI G_wGCw'C
Cuts at: 2148 3642 3727 4798 2148
Size: 1494 85 1071 2931
Fragments arranged by size:
2931 1494 1071 85
BsmI GAATG_Cn'
Cuts at: 3092 3185 3092
Size: 93 5488
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2148 2159 2245 2295 2423 2702 2742
Size: 399 358 11 86 50 128 279 40
Cuts at: 2742 2765 2900 3642 3727 4798 1391
Size: 23 135 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 358 279 135 128
86 85 50 40 23 11
BspEI T'CCGG_A
Cuts at: 5244 5244
Size: 5581
BspHI T'CATG_A
Cuts at: 3470 5200 3470
Size: 1730 3851
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2936 844
Size: 2092 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3468 1394
Size: 429 1645 3507
Fragments arranged by size:
3507 1645 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2766 4076 4250 65
Size: 1183 1518 1310 174 1396
Fragments arranged by size:
1518 1396 1310 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2061 2435 2717 4225 4412 1700
Size: 110 251 374 282 1508 187 2869
Fragments arranged by size:
2869 1508 374 282 251 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5581
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5581
BssSI C'ACGA_G
Cuts at: 2286 3641 4653 2286
Size: 1355 1012 3214
Fragments arranged by size:
3214 1355 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5581
BstBI TT'CG_AA
Cuts at: 4385 4385
Size: 5581
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5581
BstXI CCAn_nnnn'nTGG
Cuts at: 2185 2237 2185
Size: 52 5529
BstYI r'GATC_y
Cuts at: 1972 2911 3278 3663 3680 5132 5218 5230 5576
Size: 939 367 385 17 1452 86 12 346
Cuts at: 5576 1972
Size: 1977
Fragments arranged by size:
1977 1452 939 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2130 2161 2586 2619 513
Size: 552 939 126 31 425 33 3475
Fragments arranged by size:
3475 939 552 425 126 33 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2206 487
Size: 683 56 980 3862
Fragments arranged by size:
3862 980 683 56
BtsI GCAGTG_nn'
Cuts at: 3097 3891 3911 3097
Size: 794 20 4767
Fragments arranged by size:
4767 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5581
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5546
DraI TTT'AAA
Cuts at: 2885 2885
Size: 5581
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4588 809
Size: 507 3272 1802
Fragments arranged by size:
3272 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2082 2227 2532
Size: 54 1425 50 275 18 252 145 305
Cuts at: 2532 2719 3028 3917 5546 8
Size: 187 309 889 1629 43
Fragments arranged by size:
1629 1425 889 309 305 275 252 187
145 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3028 1537
Size: 275 1216 4090
Fragments arranged by size:
4090 1216 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2832 3511 1353
Size: 1479 679 3423
Fragments arranged by size:
3423 1479 679
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4168 4542 4688 387
Size: 1064 138 2579 374 146 1280
Fragments arranged by size:
2579 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2594 2831 3644 4241 5028 5296 1239
Size: 393 962 237 813 597 787 268 1524
Fragments arranged by size:
1524 962 813 787 597 393 268 237
EcoO109I rG'GnC_Cy
Cuts at: 2097 2241 2420 2097
Size: 144 179 5258
Fragments arranged by size:
5258 179 144
EcoRI G'AATT_C
Cuts at: 2890 3268 2890
Size: 378 5203
FspI TGC'GCA
Cuts at: 4087 4087
Size: 5581
HaeII r_GCGC'y
Cuts at: 1678 2081 4728 1678
Size: 403 2647 2531
Fragments arranged by size:
2647 2531 403
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2012 2044 4222 4254 4296
Size: 32 135 32 440 32 2178 32 42
Cuts at: 4296 4328 1373
Size: 32 2626
Fragments arranged by size:
2626 2178 440 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2012 2542 2647 2925 3171 669
Size: 988 281 74 530 105 278 246 3079
Fragments arranged by size:
3079 988 530 281 278 246 105 74
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5581
HpaI GTT'AAC
Cuts at: 3171 3171
Size: 5581
KpnI G_GTAC'C
Cuts at: 2906 2906
Size: 5581
MfeI C'AATT_G
Cuts at: 3180 3180
Size: 5581
MluI A'CGCG_T
Cuts at: 5457 5457
Size: 5581
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2095 2853 4695 4879 1305
Size: 790 758 1842 184 2007
Fragments arranged by size:
2007 1842 790 758 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2229 2534 10
Size: 54 1425 343 397 305 3057
Fragments arranged by size:
3057 1425 397 343 305 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2108 2618 3539 3898 4057 518
Size: 648 942 510 921 359 159 2042
Fragments arranged by size:
2042 942 921 648 510 359 159
MspA1I CmG'CkG
Cuts at: 1606 2034 2791 2997 3674 4822 5067 1606
Size: 428 757 206 677 1148 245 2120
Fragments arranged by size:
2120 1148 757 677 428 245 206
NaeI GCC'GGC
Cuts at: 2437 2437
Size: 5581
NcoI C'CATG_G
Cuts at: 513 1065 2004 2619 513
Size: 552 939 615 3475
Fragments arranged by size:
3475 939 615 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5581
NgoMIV G'CCGG_C
Cuts at: 2435 2435
Size: 5581
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5581
NotI GC'GGCC_GC
Cuts at: 3028 3028
Size: 5581
NspI r_CATG'y
Cuts at: 1132 1229 2939 4484 1132
Size: 97 1710 1545 2229
Fragments arranged by size:
2229 1710 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5581
PciI A'CATG_T
Cuts at: 4480 4480
Size: 5581
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5581
PmeI GTTT'AAAC
Cuts at: 2885 2885
Size: 5581
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3650 3682 5163 5195 3650
Size: 32 1481 32 4036
Fragments arranged by size:
4036 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2097 2097
Size: 5581
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5581
PsiI TTA'TAA
Cuts at: 3151 3151
Size: 5581
PspOMI G'GGCC_C
Cuts at: 2241 2419 2241
Size: 178 5403
PstI C_TGCA'G
Cuts at: 830 2707 2933 830
Size: 1877 226 3478
Fragments arranged by size:
3478 1877 226
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5581
PvuII CAG'CTG
Cuts at: 2791 2791
Size: 5581
SalI G'TCGA_C
Cuts at: 1936 2010 2923 1936
Size: 74 913 4594
Fragments arranged by size:
4594 913 74
SanDI GG'GwC_CC
Cuts at: 2097 2097
Size: 5581
SbfI CC_TGCA'GG
Cuts at: 2933 2933
Size: 5581
ScaI AGT'ACT
Cuts at: 3829 5541 3829
Size: 1712 3869
SfcI C'TryA_G
Cuts at: 826 1045 2703 2929 4064 4745 4936 826
Size: 219 1658 226 1135 681 191 1471
Fragments arranged by size:
1658 1471 1135 681 226 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5581
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5581
SmaI CCC'GGG
Cuts at: 1872 2657 2908 1872
Size: 785 251 4545
Fragments arranged by size:
4545 785 251
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3685 4586 4848 820
Size: 197 926 1742 901 262 1553
Fragments arranged by size:
1742 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3372 493
Size: 2879 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5581
SphI G_CATG'C
Cuts at: 1132 2939 1132
Size: 1807 3774
SspI AAT'ATT
Cuts at: 5 52 3505 5
Size: 47 3453 2081
Fragments arranged by size:
3453 2081 47
StuI AGG'CCT
Cuts at: 1517 2571 2579 1517
Size: 1054 8 4519
Fragments arranged by size:
4519 1054 8
StyI C'CwwG_G
Cuts at: 513 1065 2004 2619 3020 513
Size: 552 939 615 401 3074
Fragments arranged by size:
3074 939 615 552 401
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2174 2336 2725 3608 3625 3778 3963
Size: 1255 594 162 389 883 17 153 185
Cuts at: 3963 4408 325
Size: 445 1498
Fragments arranged by size:
1498 1255 883 594 445 389 185 162
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3827 5539 96
Size: 274 80 33 51 1443 1850 1712 138
Fragments arranged by size:
1850 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5541 5573 5541
Size: 32 5549
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5581
XbaI T'CTAG_A
Cuts at: 2917 2917
Size: 5581
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2278 2317 2278
Size: 39 5542
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5581
XmaI C'CCGG_G
Cuts at: 1870 2655 2906 1870
Size: 785 251 4545
Fragments arranged by size:
4545 785 251
XmnI GAAnn'nnTTC
Cuts at: 3710 3710
Size: 5581
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3389 276
Size: 53 83 186 936 1855 2468
Fragments arranged by size:
2468 1855 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII
SacI SacII SapI SexAI SfiI SfoI SrfI SwaI