Restriction map for
pFN21AE1875
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BamHI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BpuAI BsaAI BsaBI BsaI BsiWI
BsmI BspDI BspHI BspLU11I BssHII
BstAPI BstBI BstEII BstPI BstXI
BstYI ClaI DraI DrdI Eam1105I
EarI Eco47III EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI Ksp632I
KspI MamI MfeI MflI MluI
MunI NaeI NcoI NdeI NgoMIV
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI RcaI SacII
SalI SapI SbfI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI XbaI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AccI 1158 GTATAC GT^[AC][GT]AC
AccIII 230 TCCGGA T^CCGGA
AlwI 1020 GATCC GGATC(4/5)
AocI 391 CCTGAGG CC^T[ACGT]AGG
AspI 511 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 285 CCCGGG C^[CT]CG[GA]G
BalI 533 TGGCCA TGG^CCA
BanI 265 GGCGCC G^G[CT][GA]CC
BbeI 265 GGCGCC GGCGC^C
BglI 284 GCCCGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 673 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 673 GCTAAGC GC^T[ACGT]AGC
BseAI 230 TCCGGA T^CCGGA
BsgI 94 CTGCAC GTGCAG(16/14)
BsmBI 516 CGTCTC CGTCTC(1/5)
BsoBI 285 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1140 TGTACA T^GTACA
BspEI 230 TCCGGA T^CCGGA
BspMI 724 ACCTGC ACCTGC(4/8)
BsrDI 814 GCAATG GCAATG(2/0)
BsrFI 557 ACCGGC [GA]^CCGG[CT]
BsrGI 1140 TGTACA T^GTACA
Bst1107I 1158 GTATAC GTA^TAC
BstF5I 1137 GGATG GGATG(2/0)
BstZ17I 1158 GTATAC GTA^TAC
Bsu36I 391 CCTGAGG CC^T[ACGT]AGG
CelII 673 GCTAAGC GC^T[ACGT]AGC
Cfr10I 557 ACCGGC [GA]^CCGG[CT]
CpoI 232 CGGACCG CG^G[AT]CCG
DpnII 1020 GATC ^GATC
DraIII 526 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 796 CCACGG C^C[GA][CT]GG
Eco81I 391 CCTGAGG CC^T[ACGT]AGG
EcoNI 256 CCTCCTCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 99 CCTTGG C^C[AT][AT]GG
FokI 1137 GGATG GGATG(9/13)
KasI 265 GGCGCC G^GCGCC
MaeII 743 ACGT A^CGT
MboI 1020 GATC ^GATC
MluNI 533 TGGCCA TGG^CCA
MroI 230 TCCGGA T^CCGGA
MscI 533 TGGCCA TGG^CCA
NarI 265 GGCGCC GG^CGCC
NdeII 1020 GATC ^GATC
NheI 331 GCTAGC G^CTAGC
NotI 491 GCGGCCGC GC^GGCCGC
PflFI 511 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 342 GACCTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 616 CAGCTG CAG^CTG
RsrII 232 CGGACCG CG^G[AT]CCG
Sau3AI 1020 GATC ^GATC
ScaI 575 AGTACT AGT^ACT
SexAI 1127 ACCAGGT A^CC[AT]GGT
SfoI 265 GGCGCC GGC^GCC
SmaI 285 CCCGGG CCC^GGG
SspBI 1140 TGTACA T^GTACA
StyI 99 CCTTGG C^C[AT][AT]GG
TaqI 190 TCGA T^CGA
Tth111I 511 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 190 TCGA T^CGA
XcmI 299 CCAGCCCAGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 285 CCCGGG C^CCGGG
XmaI 285 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 103 GGCGTC G[GA]^CG[CT]C
265 GGCGCC
ApaI 242 GGGCCC GGGCC^C
414 GGGCCC
BfaI 262 CTAG C^TAG
332 CTAG
BpmI 297 CTCCAG CTGGAG(16/14)
451 CTGGAG
BsaHI 103 GGCGTC G[GA]^CG[CT]C
265 GGCGCC
BsaWI 230 TCCGGA [AT]^CCGG[AT]
235 ACCGGA
BsiEI 247 CGGCCG CG[GA][CT]^CG
492 CGGCCG
BsrBI 16 CCGCTC CCGCTC(-3/-3)
24 GAGCGG
BssSI 76 CTCGTG CACGAG(-5/-1)
457 CTCGTG
DraII 241 GGGGCCC [GA]G^G[ACGT]CC[CT]
414 GGGCCCC
EagI 247 CGGCCG C^GGCCG
492 CGGCCG
EclXI 247 CGGCCG C^GGCCG
492 CGGCCG
Eco52I 247 CGGCCG C^GGCCG
492 CGGCCG
EcoO109I 241 GGGGCCC [GA]G^G[ACGT]CC[CT]
414 GGGCCCC
Hin1I 103 GGCGTC G[GA]^CG[CT]C
265 GGCGCC
HphI 107 TCACC GGTGA(8/7)
1121 TCACC
MaeI 262 CTAG C^TAG
332 CTAG
MaeIII 106 GTCAC ^GT[ACGT]AC
740 GTGAC
MboII 483 GAAGA GAAGA(8/7)
937 GAAGA
MseI 434 TTAA T^TAA
953 TTAA
MslI 438 CATGCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
753 CATTCCAATG
NspI 437 ACATGC [GA]CATG^[CT]
974 GCATGT
PflMI 612 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1128 CCAGGTGCTGG
PstI 178 CTGCAG CTGCA^G
604 CTGCAG
SacI 226 GAGCTC GAGCT^C
454 GAGCTC
SfaNI 39 GATGC GCATC(5/9)
629 GCATC
SmlI 571 CTCAAG C^T[CT][GA]AG
1029 CTCAAG
TfiI 313 GATTC G^A[AT]TC
830 GATTC
Tru9I 434 TTAA T^TAA
953 TTAA
Tsp45I 106 GTCAC ^GT[GC]AC
740 GTGAC
Tsp509I 916 AATT ^AATT
1074 AATT
Van91I 612 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1128 CCAGGTGCTGG
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# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AspHI AvaII BanII BbvI BcnI
BmyI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DdeI EaeI EcoRII FauI
Fnu4HI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII ItaI
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NlaIII NlaIV PleI
RsaI Sau96I ScrFI SfcI TseI
TspRI