(Circular) (Six-base) MAPSORT of: pFN21AE1875.seq Check: 392 from: 1 to: 5899 pFN21AE1875 5899 bp With 182 enzymes: SgfI * July 27, 2017 10:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2738 2738 Size: 5899 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3709 278 Size: 53 83 186 936 2173 2468 Fragments arranged by size: 2468 2173 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3164 3242 1937 Size: 1227 78 4594 Fragments arranged by size: 4594 1227 78 Acc65I G'GTAC_C Cuts at: 3220 3220 Size: 5899 AclI AA'CG_TT Cuts at: 1563 4026 4399 1563 Size: 2463 373 3063 Fragments arranged by size: 3063 2463 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3962 5346 5614 1632 Size: 2330 1384 268 1917 Fragments arranged by size: 2330 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5702 AflIII A'CryG_T Cuts at: 1203 4798 5775 1203 Size: 3595 977 1327 Fragments arranged by size: 3595 1327 977 AgeI A'CCGG_T Cuts at: 4730 4730 Size: 5899 AhdI GACnn_n'nnGTC Cuts at: 4628 4628 Size: 5899 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5899 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5899 AlwNI CAG_nnn'CTG Cuts at: 2517 2623 3139 3642 5214 2517 Size: 106 516 503 1572 3202 Fragments arranged by size: 3202 1572 516 503 106 ApaI G_GGCC'C Cuts at: 2251 2423 2251 Size: 172 5727 ApaLI G'TGCA_C Cuts at: 3956 5112 3956 Size: 1156 4743 ApoI r'AATT_y Cuts at: 1493 3208 3434 3586 5695 1493 Size: 1715 226 152 2109 1697 Fragments arranged by size: 2109 1715 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4453 160 Size: 872 3421 1606 Fragments arranged by size: 3421 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5899 AvaI C'yCGr_G Cuts at: 1870 1943 2290 3224 1870 Size: 73 347 934 4545 Fragments arranged by size: 4545 934 347 73 BamHI G'GATC_C Cuts at: 3229 3596 3229 Size: 367 5532 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2270 3220 4675 618 Size: 325 217 627 483 950 1455 1842 Fragments arranged by size: 1842 1455 950 627 483 325 217 BanII G_rGCy'C Cuts at: 1391 2235 2251 2423 2463 3218 1391 Size: 844 16 172 40 755 4072 Fragments arranged by size: 4072 844 755 172 40 16 BbeI G_GCGC'C Cuts at: 2274 2274 Size: 5899 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4928 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4090 4124 1903 Size: 34 2153 34 3678 Fragments arranged by size: 3678 2153 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3791 5001 3791 Size: 1210 4689 BclI T'GATC_A Cuts at: 1548 1548 Size: 5899 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2295 4510 136 Size: 107 122 71 631 605 623 2215 1525 Fragments arranged by size: 2215 1525 631 623 605 122 107 71 BglII A'GATC_T Cuts at: 5894 5894 Size: 5899 BlpI GC'TnA_GC Cuts at: 2679 3316 2679 Size: 637 5262 Bme1580I G_kGCm'C Cuts at: 1790 2251 2364 2423 3960 5116 1790 Size: 461 113 59 1537 1156 2573 Fragments arranged by size: 2573 1537 1156 461 113 59 BmgBI CAC'GTC Cuts at: 1318 2749 1318 Size: 1431 4468 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2303 2351 2597 Size: 416 53 83 399 24 886 48 246 Cuts at: 2597 3630 4588 442 Size: 1033 958 1753 Fragments arranged by size: 1753 1033 958 886 416 399 246 83 53 48 24 BmtI G_CTAG'C Cuts at: 1056 2340 1056 Size: 1284 4615 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2287 2477 4559 1239 Size: 1048 190 2082 2579 Fragments arranged by size: 2579 2082 1048 190 Bpu10I CC'TnA_GC Cuts at: 2027 2144 2027 Size: 117 5782 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2561 3019 4024 4889 5187 2561 Size: 458 1005 865 298 3273 Fragments arranged by size: 3273 1005 865 458 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4562 882 Size: 617 3063 2219 Fragments arranged by size: 3063 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3690 493 Size: 711 2486 2702 Fragments arranged by size: 2702 2486 711 BsaBI GATnn'nnATC Cuts at: 5893 5893 Size: 5899 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2109 2271 3706 4088 Size: 53 83 186 936 576 162 1435 382 Cuts at: 4088 4723 275 Size: 635 1451 Fragments arranged by size: 1451 1435 936 635 576 382 186 162 83 53 BsaWI w'CCGG_w Cuts at: 2235 2240 4332 4730 5004 5151 5562 2235 Size: 5 2092 398 274 147 411 2572 Fragments arranged by size: 2572 2092 411 398 274 147 5 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5674 Fragments arranged by size: 5674 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2253 2266 2414 2517 2675 2952 1186 Size: 1067 13 148 103 158 277 4133 Fragments arranged by size: 4133 1067 277 158 148 103 13 BseYI C'CCAG_C Cuts at: 2280 2931 5102 5598 2280 Size: 651 2171 496 2581 Fragments arranged by size: 2581 2171 651 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2084 1163 Size: 573 348 4978 Fragments arranged by size: 4978 573 348 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2255 2500 3349 4110 5138 1540 Size: 275 186 254 245 849 761 1028 2301 Fragments arranged by size: 2301 1028 849 761 275 254 245 186 BsiHKAI G_wGCw'C Cuts at: 2235 2463 2574 3960 4045 5116 2235 Size: 228 111 1386 85 1071 3018 Fragments arranged by size: 3018 1386 1071 228 111 85 BsmI GAATG_Cn' Cuts at: 3410 3503 3410 Size: 93 5806 BsmBI CGTCTCn'nnnn_ Cuts at: 2527 2527 Size: 5899 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2235 2251 2364 2423 2463 2574 3218 Size: 399 445 16 113 59 40 111 644 Cuts at: 3218 3960 4045 5116 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 644 445 399 113 111 85 59 40 16 BspEI T'CCGG_A Cuts at: 2235 5562 2235 Size: 3327 2572 BspHI T'CATG_A Cuts at: 3788 5518 3788 Size: 1730 4169 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2738 3254 844 Size: 1894 516 3489 Fragments arranged by size: 3489 1894 516 BsrBI CCG'CTC Cuts at: 1394 1823 2023 2031 3786 1394 Size: 429 200 8 1755 3507 Fragments arranged by size: 3507 1755 429 200 8 BsrDI GCAATG_nn' Cuts at: 65 1248 2826 4394 4568 65 Size: 1183 1578 1568 174 1396 Fragments arranged by size: 1578 1568 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2562 4543 4730 1700 Size: 110 752 1981 187 2869 Fragments arranged by size: 2869 1981 752 187 110 BsrGI T'GTAC_A Cuts at: 96 3145 96 Size: 3049 2850 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5899 BssSI C'ACGA_G Cuts at: 2081 2462 3959 4971 2081 Size: 381 1497 1012 3009 Fragments arranged by size: 3009 1497 1012 381 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5899 BstBI TT'CG_AA Cuts at: 4703 4703 Size: 5899 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5899 BstYI r'GATC_y Cuts at: 1972 3229 3596 3981 3998 5450 5536 5548 5894 Size: 1257 367 385 17 1452 86 12 346 Cuts at: 5894 1972 Size: 1977 Fragments arranged by size: 1977 1452 1257 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3165 3165 Size: 5899 Bsu36I CC'TnA_GG Cuts at: 2397 2397 Size: 5899 BtgI C'CryG_G Cuts at: 513 1065 2801 513 Size: 552 1736 3611 Fragments arranged by size: 3611 1736 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2057 487 Size: 683 56 831 4329 Fragments arranged by size: 4329 831 683 56 BtsI GCAGTG_nn' Cuts at: 3415 4209 4229 3415 Size: 794 20 5085 Fragments arranged by size: 5085 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5899 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2985 3020 529 Size: 35 2421 35 3408 Fragments arranged by size: 3408 2421 35 35 DraI TTT'AAA Cuts at: 3203 3203 Size: 5899 DraIII CAC_nnn'GTG Cuts at: 2536 2536 Size: 5899 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4906 809 Size: 507 3590 1802 Fragments arranged by size: 3590 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2085 2134 2252 Size: 54 1425 50 275 18 255 49 118 Cuts at: 2252 2497 2538 3346 4235 5864 8 Size: 245 41 808 889 1629 43 Fragments arranged by size: 1629 1425 889 808 275 255 245 118 54 50 49 43 41 18 EagI C'GGCC_G Cuts at: 1537 1812 2252 2497 3346 1537 Size: 275 440 245 849 4090 Fragments arranged by size: 4090 849 440 275 245 EarI CTCTTCn'nnn_ Cuts at: 1353 3829 1353 Size: 2476 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3017 4486 4860 5006 387 Size: 1064 138 1428 1469 374 146 1280 Fragments arranged by size: 1469 1428 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2233 2461 2233 Size: 228 5671 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2287 2477 3962 4559 5346 5614 1239 Size: 393 655 190 1485 597 787 268 1524 Fragments arranged by size: 1524 1485 787 655 597 393 268 190 EcoNI CCTnn'n_nnAGG Cuts at: 2265 2265 Size: 5899 EcoO109I rG'GnC_Cy Cuts at: 2247 2420 2247 Size: 173 5726 EcoRI G'AATT_C Cuts at: 3208 3586 3208 Size: 378 5521 FspI TGC'GCA Cuts at: 4405 4405 Size: 5899 HaeII r_GCGC'y Cuts at: 1678 2040 2274 2617 5046 1678 Size: 362 234 343 2429 2531 Fragments arranged by size: 2531 2429 362 343 234 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2507 2539 4540 4572 4614 Size: 32 135 32 935 32 2001 32 42 Cuts at: 4614 4646 1373 Size: 32 2626 Fragments arranged by size: 2626 2001 935 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3243 3489 669 Size: 988 281 1305 246 3079 Fragments arranged by size: 3079 1305 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5899 HpaI GTT'AAC Cuts at: 3489 3489 Size: 5899 KasI G'GCGC_C Cuts at: 2270 2270 Size: 5899 KpnI G_GTAC'C Cuts at: 3224 3224 Size: 5899 MfeI C'AATT_G Cuts at: 3498 3498 Size: 5899 MluI A'CGCG_T Cuts at: 5775 5775 Size: 5899 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2146 5013 5197 1305 Size: 841 2867 184 2007 Fragments arranged by size: 2867 2007 841 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2540 10 Size: 54 1425 343 708 3369 Fragments arranged by size: 3369 1425 708 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2447 2762 3857 4216 4375 518 Size: 648 1281 315 1095 359 159 2042 Fragments arranged by size: 2042 1281 1095 648 359 315 159 MspA1I CmG'CkG Cuts at: 1606 2065 2182 2410 2623 3315 3992 5140 5385 Size: 459 117 228 213 692 677 1148 245 Cuts at: 5385 1606 Size: 2120 Fragments arranged by size: 2120 1148 692 677 459 245 228 213 117 NarI GG'CG_CC Cuts at: 2271 2271 Size: 5899 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5347 NdeI CA'TA_TG Cuts at: 387 387 Size: 5899 NheI G'CTAG_C Cuts at: 1052 2336 1052 Size: 1284 4615 NotI GC'GGCC_GC Cuts at: 2497 3346 2497 Size: 849 5050 NspI r_CATG'y Cuts at: 1132 1229 2446 2983 3257 4802 1132 Size: 97 1217 537 274 1545 2229 Fragments arranged by size: 2229 1545 1217 537 274 97 PasI CC'CwG_GG Cuts at: 1348 2511 1348 Size: 1163 4736 PciI A'CATG_T Cuts at: 4798 4798 Size: 5899 PflMI CCAn_nnn'nTGG Cuts at: 1108 2623 3139 1108 Size: 1515 516 3868 Fragments arranged by size: 3868 1515 516 PmeI GTTT'AAAC Cuts at: 3203 3203 Size: 5899 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3968 4000 5481 5513 3968 Size: 32 1481 32 4354 Fragments arranged by size: 4354 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2351 1613 Size: 738 5161 PsiI TTA'TAA Cuts at: 3469 3469 Size: 5899 PspOMI G'GGCC_C Cuts at: 2247 2419 2247 Size: 172 5727 PstI C_TGCA'G Cuts at: 830 2187 2613 3251 830 Size: 1357 426 638 3478 Fragments arranged by size: 3478 1357 638 426 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5899 PvuII CAG'CTG Cuts at: 2623 2623 Size: 5899 RsrII CG'GwC_CG Cuts at: 2238 2238 Size: 5899 SacI G_AGCT'C Cuts at: 2235 2463 2235 Size: 228 5671 SalI G'TCGA_C Cuts at: 1936 3241 1936 Size: 1305 4594 SbfI CC_TGCA'GG Cuts at: 3251 3251 Size: 5899 ScaI AGT'ACT Cuts at: 2582 4147 5859 2582 Size: 1565 1712 2622 Fragments arranged by size: 2622 1712 1565 SexAI A'CCwGG_T Cuts at: 3132 3132 Size: 5899 SfcI C'TryA_G Cuts at: 826 1045 2060 2183 2609 3247 4382 5063 5254 Size: 219 1015 123 426 638 1135 681 191 Cuts at: 5254 826 Size: 1471 Fragments arranged by size: 1471 1135 1015 681 638 426 219 191 123 SfoI GGC'GCC Cuts at: 2272 2272 Size: 5899 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5899 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5899 SmaI CCC'GGG Cuts at: 1872 2292 3226 1872 Size: 420 934 4545 Fragments arranged by size: 4545 934 420 SmlI C'TyrA_G Cuts at: 820 1017 1943 2576 3034 4003 4904 5166 820 Size: 197 926 633 458 969 901 262 1553 Fragments arranged by size: 1553 969 926 901 633 458 262 197 SnaBI TAC'GTA Cuts at: 493 3690 493 Size: 3197 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5899 SphI G_CATG'C Cuts at: 1132 3257 1132 Size: 2125 3774 SspI AAT'ATT Cuts at: 5 52 3823 5 Size: 47 3771 2081 Fragments arranged by size: 3771 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5899 StyI C'CwwG_G Cuts at: 513 1065 2104 3338 513 Size: 552 1039 1234 3074 Fragments arranged by size: 3074 1234 1039 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2341 2616 3090 3926 3943 4096 4281 Size: 1255 761 275 474 836 17 153 185 Cuts at: 4281 4726 325 Size: 445 1498 Fragments arranged by size: 1498 1255 836 761 474 445 275 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2580 3145 4145 Size: 274 80 33 51 1443 603 565 1000 Cuts at: 4145 5857 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1000 603 565 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5859 5891 5859 Size: 32 5867 Tth111I GACn'n_nGTC Cuts at: 1316 2519 1316 Size: 1203 4696 XbaI T'CTAG_A Cuts at: 3235 3235 Size: 5899 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2311 2311 Size: 5899 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5899 XmaI C'CCGG_G Cuts at: 1870 2290 3224 1870 Size: 420 934 4545 Fragments arranged by size: 4545 934 420 XmnI GAAnn'nnTTC Cuts at: 4028 4028 Size: 5899 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3707 276 Size: 53 83 186 936 2173 2468 Fragments arranged by size: 2468 2173 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstXI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI SacII SanDI SapI SfiI SrfI SwaI