Restriction map for
pFN21AE1845
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BamHI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiWI BsmFI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraII DraIII DrdI DsaI
Eam1105I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MslI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TaqI TthHB8I Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcsI 569 AAATTT [GA]^AATT[CT]
AlwI 646 GGATC GGATC(4/5)
Aor51HI 63 AGCGCT AGC^GCT
ApoI 569 AAATTT [GA]^AATT[CT]
AspI 387 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 381 GGACC G^G[AT]CC
BalI 95 TGGCCA TGG^CCA
BanI 834 GGCACC G^G[CT][GA]CC
BglI 955 GCCACCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseRI 261 GAGGAG GAGGAG(10/8)
BsgI 995 CTGCAC GTGCAG(16/14)
BsiEI 56 CGGCCG CG[GA][CT]^CG
BsiYI 194 CCGGCGGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 194 CCGGCGGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 283 GTCTC GTCTC(1/5)
BsmBI 282 CGTCTC CGTCTC(1/5)
BsrBI 204 GAGCGG CCGCTC(-3/-3)
BsrFI 193 ACCGGC [GA]^CCGG[CT]
BstF5I 692 CATCC GGATG(2/0)
BstYI 646 GGATCT [GA]^GATC[CT]
Cfr10I 193 ACCGGC [GA]^CCGG[CT]
DpnII 647 GATC ^GATC
EagI 56 CGGCCG C^GGCCG
EclXI 56 CGGCCG C^GGCCG
Eco47III 63 AGCGCT AGC^GCT
Eco52I 56 CGGCCG C^GGCCG
FokI 692 CATCC GGATG(9/13)
MaeII 39 ACGT A^CGT
MboI 647 GATC ^GATC
MflI 646 GGATCT [GA]^GATC[CT]
MluNI 95 TGGCCA TGG^CCA
MscI 95 TGGCCA TGG^CCA
NdeII 647 GATC ^GATC
NlaIII 806 CATG CATG^
NotI 55 GCGGCCGC GC^GGCCGC
PflFI 387 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 550 GAGCTC GAGCT^C
Sau3AI 647 GATC ^GATC
SgrAI 192 CACCGGCG C[GA]^CCGG[CT]G
SmaI 20 CCCGGG CCC^GGG
Tth111I 387 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 852 CCAAAGTGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 646 GGATCT [GA]^GATC[CT]
XmaCI 20 CCCGGG C^CCGGG
XmaI 20 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccII 69 CGCG CG^CG
90 CGCG
AlwNI 156 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1000 CAGCAACTG
Asp700I 433 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
979 GAAGATGTTC
AspHI 352 GAGCAC G[AT]GC[AT]^C
550 GAGCTC
BanII 288 GGGCTC G[GA]GC[CT]^C
550 GAGCTC
BpmI 880 CTGGAG CTGGAG(16/14)
1006 CTGGAG
BsiHKAI 352 GAGCAC G[AT]GC[AT]^C
550 GAGCTC
BstUI 69 CGCG CG^CG
90 CGCG
DdeI 638 CTAAG C^T[ACGT]AG
763 CTCAG
EaeI 56 CGGCCG [CT]^GGCC[GA]
95 TGGCCA
EarI 273 CTCTTC CTCTTC(1/4)
616 GAAGAG
HgaI 152 GCGTC GACGC(5/10)
281 GCGTC
Ksp632I 273 CTCTTC CTCTTC(1/4)
616 GAAGAG
MvnI 69 CGCG CG^CG
90 CGCG
SfaNI 483 GATGC GCATC(5/9)
778 GATGC
SmlI 583 CTTGAG C^T[CT][GA]AG
1021 CTTGAG
XmnI 433 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
979 GAAGATGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AfaI AluI AvaI BbvI
BcnI BfaI BmyI BsaJI BsoBI
Bsp1286I BsrI BssKI BstNI Cac8I
CfoI Cfr13I EcoRII FauI Fnu4HI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeIII MboII MnlI MseI MspA1I
MspI MvaI MwoI NciI NlaIV
PleI PvuII RsaI Sau96I ScrFI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI