(Circular) (Six-base) MAPSORT of: pFN21AE1845.seq Check: 9705 from: 1 to: 5788 pFN21AE1845 5788 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3598 278 Size: 53 83 186 936 2062 2468 Fragments arranged by size: 2468 2062 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3131 1937 Size: 1194 4594 Acc65I G'GTAC_C Cuts at: 3109 3109 Size: 5788 AclI AA'CG_TT Cuts at: 1563 3915 4288 1563 Size: 2352 373 3063 Fragments arranged by size: 3063 2352 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2429 2742 3851 5235 5503 1632 Size: 797 313 1109 1384 268 1917 Fragments arranged by size: 1917 1384 1109 797 313 268 AfeI AGC'GCT Cuts at: 2070 2070 Size: 5788 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5591 AflIII A'CryG_T Cuts at: 1203 4687 5664 1203 Size: 3484 977 1327 Fragments arranged by size: 3484 1327 977 AgeI A'CCGG_T Cuts at: 4619 4619 Size: 5788 AhdI GACnn_n'nnGTC Cuts at: 4517 4517 Size: 5788 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5788 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5788 AlwNI CAG_nnn'CTG Cuts at: 2166 3010 3531 5103 2166 Size: 844 521 1572 2851 Fragments arranged by size: 2851 1572 844 521 ApaLI G'TGCA_C Cuts at: 3845 5001 3845 Size: 1156 4632 ApoI r'AATT_y Cuts at: 1493 2574 3097 3323 3475 5584 1493 Size: 1081 523 226 152 2109 1697 Fragments arranged by size: 2109 1697 1081 523 226 152 AseI AT'TA_AT Cuts at: 160 1032 4342 160 Size: 872 3310 1606 Fragments arranged by size: 3310 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5788 AvaI C'yCGr_G Cuts at: 1870 1943 2025 2290 2834 3113 1870 Size: 73 82 265 544 279 4545 Fragments arranged by size: 4545 544 279 265 82 73 BamHI G'GATC_C Cuts at: 3118 3485 3118 Size: 367 5421 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2839 3109 4564 618 Size: 325 217 627 1052 270 1455 1842 Fragments arranged by size: 1842 1455 1052 627 325 270 217 BanII G_rGCy'C Cuts at: 1391 2297 2559 3107 1391 Size: 906 262 548 4072 Fragments arranged by size: 4072 906 548 262 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4817 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3979 4013 1903 Size: 34 2042 34 3678 Fragments arranged by size: 3678 2042 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3680 4890 3680 Size: 1210 4578 BclI T'GATC_A Cuts at: 1548 1548 Size: 5788 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2966 4399 136 Size: 107 122 71 631 605 1294 1433 1525 Fragments arranged by size: 1525 1433 1294 631 605 122 107 71 BglII A'GATC_T Cuts at: 5783 5783 Size: 5788 BlpI GC'TnA_GC Cuts at: 3205 3205 Size: 5788 Bme1580I G_kGCm'C Cuts at: 1790 3849 5005 1790 Size: 2059 1156 2573 Fragments arranged by size: 2573 2059 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5788 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3519 4477 442 Size: 416 53 83 399 24 2102 958 1753 Fragments arranged by size: 2102 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5788 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2906 3032 4448 1239 Size: 1667 126 1416 2579 Fragments arranged by size: 2579 1667 1416 126 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2609 3047 3913 4778 5076 2609 Size: 438 866 865 298 3321 Fragments arranged by size: 3321 866 865 438 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4451 882 Size: 617 2952 2219 Fragments arranged by size: 2952 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3579 493 Size: 711 2375 2702 Fragments arranged by size: 2702 2375 711 BsaBI GATnn'nnATC Cuts at: 5782 5782 Size: 5788 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3595 3977 4612 275 Size: 53 83 186 936 2062 382 635 1451 Fragments arranged by size: 2062 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4221 4619 4893 5040 5451 4221 Size: 398 274 147 411 4558 Fragments arranged by size: 4558 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5563 Fragments arranged by size: 5563 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2281 1186 Size: 1095 4693 BseYI C'CCAG_C Cuts at: 4991 5487 4991 Size: 496 5292 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2985 1163 Size: 573 1249 3966 Fragments arranged by size: 3966 1249 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2064 3238 3999 5027 1540 Size: 275 186 63 1174 761 1028 2301 Fragments arranged by size: 2301 1174 1028 761 275 186 63 BsiHKAI G_wGCw'C Cuts at: 2361 2559 3849 3934 5005 2361 Size: 198 1290 85 1071 3144 Fragments arranged by size: 3144 1290 1071 198 85 BsmI GAATG_Cn' Cuts at: 3299 3392 3299 Size: 93 5695 BsmBI CGTCTCn'nnnn_ Cuts at: 2293 2293 Size: 5788 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2297 2361 2559 3107 3849 3934 5005 Size: 399 507 64 198 548 742 85 1071 Cuts at: 5005 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 548 507 399 198 85 64 BspEI T'CCGG_A Cuts at: 5451 5451 Size: 5788 BspHI T'CATG_A Cuts at: 3677 5407 3677 Size: 1730 4058 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3143 844 Size: 2299 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2211 3675 1394 Size: 429 388 1464 3507 Fragments arranged by size: 3507 1464 429 388 BsrDI GCAATG_nn' Cuts at: 65 1248 4283 4457 65 Size: 1183 3035 174 1396 Fragments arranged by size: 3035 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2198 4432 4619 1700 Size: 110 388 2234 187 2869 Fragments arranged by size: 2869 2234 388 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5788 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5788 BssSI C'ACGA_G Cuts at: 3848 4860 3848 Size: 1012 4776 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5788 BstBI TT'CG_AA Cuts at: 4592 4592 Size: 5788 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5788 BstYI r'GATC_y Cuts at: 1972 2651 3118 3485 3870 3887 5339 5425 5437 Size: 679 467 367 385 17 1452 86 12 Cuts at: 5437 5783 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 679 467 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5236 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5049 Fragments arranged by size: 5049 683 56 BtsI GCAGTG_nn' Cuts at: 2119 2456 3304 4098 4118 2119 Size: 337 848 794 20 3789 Fragments arranged by size: 3789 848 794 337 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5788 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2167 2202 529 Size: 35 1603 35 4115 Fragments arranged by size: 4115 1603 35 35 DraI TTT'AAA Cuts at: 3092 3092 Size: 5788 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4795 809 Size: 507 3479 1802 Fragments arranged by size: 3479 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2061 2100 3235 Size: 54 1425 50 275 18 231 39 1135 Cuts at: 3235 4124 5753 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1135 889 275 231 54 50 43 39 18 EagI C'GGCC_G Cuts at: 1537 1812 2061 3235 1537 Size: 275 249 1174 4090 Fragments arranged by size: 4090 1174 275 249 EarI CTCTTCn'nnn_ Cuts at: 1353 2284 2616 3718 1353 Size: 931 332 1102 3423 Fragments arranged by size: 3423 1102 931 332 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4375 4749 4895 387 Size: 1064 138 2786 374 146 1280 Fragments arranged by size: 2786 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2557 2557 Size: 5788 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2429 2742 2906 3032 3851 4448 5235 Size: 393 797 313 164 126 819 597 787 Cuts at: 5235 5503 1239 Size: 268 1524 Fragments arranged by size: 1524 819 797 787 597 393 313 268 164 126 EcoRI G'AATT_C Cuts at: 3097 3475 3097 Size: 378 5410 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2459 2459 Size: 5788 FspI TGC'GCA Cuts at: 4294 4294 Size: 5788 HaeII r_GCGC'y Cuts at: 1678 2072 2142 2257 4935 1678 Size: 394 70 115 2678 2531 Fragments arranged by size: 2678 2531 394 115 70 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2546 2578 4429 4461 4503 Size: 32 135 32 974 32 1851 32 42 Cuts at: 4503 4535 1373 Size: 32 2626 Fragments arranged by size: 2626 1851 974 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3132 3378 669 Size: 988 281 1194 246 3079 Fragments arranged by size: 3079 1194 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5788 HpaI GTT'AAC Cuts at: 3378 3378 Size: 5788 KpnI G_GTAC'C Cuts at: 3113 3113 Size: 5788 MfeI C'AATT_G Cuts at: 3387 3387 Size: 5788 MluI A'CGCG_T Cuts at: 5664 5664 Size: 5788 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4902 5086 1305 Size: 3597 184 2007 Fragments arranged by size: 3597 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2102 10 Size: 54 1425 343 270 3696 Fragments arranged by size: 3696 1425 343 270 54 MslI CAynn'nnrTG Cuts at: 518 1166 3746 4105 4264 518 Size: 648 2580 359 159 2042 Fragments arranged by size: 2580 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2106 2163 2220 2828 3204 3881 5029 5274 Size: 500 57 57 608 376 677 1148 245 Cuts at: 5274 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 608 500 376 245 57 57 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5236 NdeI CA'TA_TG Cuts at: 387 387 Size: 5788 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5788 NotI GC'GGCC_GC Cuts at: 2061 3235 2061 Size: 1174 4614 NspI r_CATG'y Cuts at: 1132 1229 3146 4691 1132 Size: 97 1917 1545 2229 Fragments arranged by size: 2229 1917 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5788 PciI A'CATG_T Cuts at: 4687 4687 Size: 5788 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5788 PmeI GTTT'AAAC Cuts at: 3092 3092 Size: 5788 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3857 3889 5370 5402 3857 Size: 32 1481 32 4243 Fragments arranged by size: 4243 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5788 PsiI TTA'TAA Cuts at: 3358 3358 Size: 5788 PstI C_TGCA'G Cuts at: 830 3140 830 Size: 2310 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5788 PvuII CAG'CTG Cuts at: 2163 2220 2828 2163 Size: 57 608 5123 Fragments arranged by size: 5123 608 57 SacI G_AGCT'C Cuts at: 2559 2559 Size: 5788 SalI G'TCGA_C Cuts at: 1936 3130 1936 Size: 1194 4594 SbfI CC_TGCA'GG Cuts at: 3140 3140 Size: 5788 ScaI AGT'ACT Cuts at: 4036 5748 4036 Size: 1712 4076 SfcI C'TryA_G Cuts at: 826 1045 3136 4271 4952 5143 826 Size: 219 2091 1135 681 191 1471 Fragments arranged by size: 2091 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5788 SgrAI Cr'CCGG_yG Cuts at: 1700 2198 1700 Size: 498 5290 SmaI CCC'GGG Cuts at: 1872 2027 3115 1872 Size: 155 1088 4545 Fragments arranged by size: 4545 1088 155 SmlI C'TyrA_G Cuts at: 820 1017 1943 2588 3026 3892 4793 5055 820 Size: 197 926 645 438 866 901 262 1553 Fragments arranged by size: 1553 926 901 866 645 438 262 197 SnaBI TAC'GTA Cuts at: 493 3579 493 Size: 3086 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5788 SphI G_CATG'C Cuts at: 1132 3146 1132 Size: 2014 3774 SspI AAT'ATT Cuts at: 5 52 3712 5 Size: 47 3660 2081 Fragments arranged by size: 3660 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5788 StyI C'CwwG_G Cuts at: 513 1065 3227 513 Size: 552 2162 3074 Fragments arranged by size: 3074 2162 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3815 3832 3985 4170 4615 325 Size: 1255 2235 17 153 185 445 1498 Fragments arranged by size: 2235 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2764 4034 5746 Size: 274 80 33 51 1443 787 1270 1712 Cuts at: 5746 96 Size: 138 Fragments arranged by size: 1712 1443 1270 787 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5748 5780 5748 Size: 32 5756 Tth111I GACn'n_nGTC Cuts at: 1316 2395 1316 Size: 1079 4709 XbaI T'CTAG_A Cuts at: 3124 3124 Size: 5788 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2864 2864 Size: 5788 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5788 XmaI C'CCGG_G Cuts at: 1870 2025 3113 1870 Size: 155 1088 4545 Fragments arranged by size: 4545 1088 155 XmnI GAAnn'nnTTC Cuts at: 2442 2988 3917 2442 Size: 546 929 4313 Fragments arranged by size: 4313 929 546 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3596 276 Size: 53 83 186 936 2062 2468 Fragments arranged by size: 2468 2062 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI