################################## # NO CUT ENZYMES ( 151 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AviII AvrII BalI BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BfrI BglI BglII BlnI BpmI BpuAI BsaAI BsaBI BsaHI BseAI BsgI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI HphI KasI KpnI KspI MaeII MamI MfeI MflI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI TspRI XbaI XcmI XhoI XhoII ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AflIII 261 ACATGT A^C[GA][CT]GT AlwI 64 GATCC GGATC(4/5) Asp700I 380 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 676 GAGCAC G[AT]GC[AT]^C AspI 700 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 536 CCCGGG C^[CT]CG[GA]G BbvI 189 GCAGC GCAGC(8/12) BciVI 102 GGATAC GTATCC(6/5) BclI 330 TGATCA T^GATCA BfaI 475 CTAG C^TAG BlpI 421 GCTGAGC GC^T[ACGT]AGC BmyI 676 GAGCAC G[AGT]GC[ACT]^C Bpu1102I 421 GCTGAGC GC^T[ACGT]AGC BsaI 357 GAGACC GGTCTC(1/5) BsaWI 482 TCCGGT [AT]^CCGG[AT] BsiHKAI 676 GAGCAC G[AT]GC[AT]^C BsoBI 536 CCCGGG C^[CT]CG[GA]G Bsp1286I 676 GAGCAC G[AGT]GC[ACT]^C Bsp1407I 256 TGTACA T^GTACA BspLU11I 261 ACATGT A^CATGT BsrGI 256 TGTACA T^GTACA BsrI 822 CCAGT ACTGG(1/-1) BssSI 679 CACGAG CACGAG(-5/-1) Cac8I 116 GCAAGC GC[ACGT]^[ACGT]GC CelII 421 GCTGAGC GC^T[ACGT]AGC DraII 519 GGGACCT [GA]G^G[ACGT]CC[CT] DsaI 647 CCATGG C^C[GA][CT]GG EarI 247 GAAGAG CTCTTC(1/4) EcoNI 766 CCTCAGATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 519 GGGACCT [GA]G^G[ACGT]CC[CT] EcoT14I 647 CCATGG C^C[AT][AT]GG FbaI 330 TGATCA T^GATCA Fnu4HI 189 GCAGC GC^[ACGT]GC HincII 214 GTCAAC GT[CT]^[GA]AC HindII 214 GTCAAC GT[CT]^[GA]AC ItaI 189 GCAGC GC^[ACGT]GC Ksp632I 247 GAAGAG CTCTTC(1/4) MaeI 475 CTAG C^TAG NcoI 647 CCATGG C^CATGG NspI 261 ACATGT [GA]CATG^[CT] PflFI 700 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 779 CCACAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 479 GACTC GAGTC(4/5) PpuMI 519 GGGACCT [GA]G^G[AT]CC[CT] SmaI 536 CCCGGG CCC^GGG SmlI 291 CTTGAG C^T[CT][GA]AG SspBI 256 TGTACA T^GTACA StuI 54 AGGCCT AGG^CCT StyI 647 CCATGG C^C[AT][AT]GG TaqI 515 TCGA T^CGA TfiI 303 GATTC G^A[AT]TC TseI 189 GCAGC G^C[AT]GC Tth111I 700 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 515 TCGA T^CGA Van91I 779 CCACAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 536 CCCGGG C^CCGGG XmaI 536 CCCGGG C^CCGGG XmnI 380 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AciI 23 CCGC CCGC(-3/-1) 571 GCGG AcsI 71 AAATTT [GA]^AATT[CT] 626 GAATTT AfaI 257 GTAC GT^AC 560 GTAC ApoI 71 AAATTT [GA]^AATT[CT] 626 GAATTT BanI 10 GGCACC G^G[CT][GA]CC 485 GGTGCC BseRI 298 GAGGAG GAGGAG(10/8) 398 GAGGAG BsmAI 357 GAGAC GTCTC(1/5) 460 GAGAC BsmFI 499 GGGAC GGGAC(10/14) 519 GGGAC DpnII 64 GATC ^GATC 331 GATC HindIII 546 AAGCTT A^AGCTT 744 AAGCTT HinfI 303 GATTC G^A[ACGT]TC 479 GACTC MboI 64 GATC ^GATC 331 GATC MseI 133 TTAA T^TAA 709 TTAA NdeII 64 GATC ^GATC 331 GATC PshAI 359 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 501 GACAATGGTC RsaI 257 GTAC GT^AC 560 GTAC Sau3AI 64 GATC ^GATC 331 GATC SfaNI 127 GATGC GCATC(5/9) 164 GCATC Tru9I 133 TTAA T^TAA 709 TTAA Tsp45I 271 GTGAC ^GT[GC]AC 508 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 29 enzymes) # ######################################### AluI AvaII BcnI BsaJI BsiYI BslI BssKI BstF5I BstNI Cfr13I DdeI EcoRII FokI HaeIII HapII HpaII MaeIII MboII MnlI MslI MspI MvaI MwoI NciI NlaIII NlaIV Sau96I ScrFI Tsp509I