################################## # NO CUT ENZYMES ( 181 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BlnI BpmI BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaI HpaII KasI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MslI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tth111I TthHB8I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AcsI 316 AAATTC [GA]^AATT[CT] AlwI 246 GATCC GGATC(4/5) AlwNI 337 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 316 AAATTC [GA]^AATT[CT] Asp700I 429 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 37 GTGCTC G[AT]GC[AT]^C AvaII 107 GGACC G^G[AT]CC BfaI 310 CTAG C^TAG BlpI 39 GCTCAGC GC^T[ACGT]AGC BmyI 37 GTGCTC G[AGT]GC[ACT]^C Bpu1102I 39 GCTCAGC GC^T[ACGT]AGC BsaI 301 GAGACC GGTCTC(1/5) BsiHKAI 37 GTGCTC G[AT]GC[AT]^C Bsp1286I 37 GTGCTC G[AGT]GC[ACT]^C BspHI 509 TCATGA T^CATGA CelII 39 GCTCAGC GC^T[ACGT]AGC DdeI 40 CTCAG C^T[ACGT]AG DraII 106 AGGACCT [GA]G^G[ACGT]CC[CT] EarI 265 CTCTTC CTCTTC(1/4) EcoNI 396 CCTGATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 106 AGGACCT [GA]G^G[ACGT]CC[CT] HincII 528 GTCAAC GT[CT]^[GA]AC HindII 528 GTCAAC GT[CT]^[GA]AC HphI 381 GGTGA GGTGA(8/7) Ksp632I 265 CTCTTC CTCTTC(1/4) MaeI 310 CTAG C^TAG MaeIII 382 GTGAC ^GT[ACGT]AC MseI 550 TTAA T^TAA PflMI 87 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 106 AGGACCT [GA]G^G[AT]CC[CT] RcaI 509 TCATGA T^CATGA SfaNI 114 GATGC GCATC(5/9) Tru9I 550 TTAA T^TAA Tsp45I 382 GTGAC ^GT[GC]AC Tsp509I 317 AATT ^AATT TspRI 324 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 87 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 186 CCAGGAGGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 429 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### BglII 254 AGATCT A^GATCT 514 AGATCT BseRI 132 GAGGAG GAGGAG(10/8) 190 GAGGAG BsiYI 87 CCACAGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 396 CCTGATGAAGG BslI 87 CCACAGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 396 CCTGATGAAGG BsmAI 301 GAGAC GTCTC(1/5) 388 GAGAC BsrI 21 ACTGG ACTGG(1/-1) 204 CCAGT BstF5I 225 CATCC GGATG(2/0) 297 GGATG BstYI 254 AGATCT [GA]^GATC[CT] 514 AGATCT Cfr13I 27 GGCCC G^G[ACGT]CC 107 GGACC FokI 225 CATCC GGATG(9/13) 297 GGATG HaeIII 3 GGCC GG^CC 27 GGCC MflI 254 AGATCT [GA]^GATC[CT] 514 AGATCT MspA1I 42 CAGCTG C[AC]G^C[GT]G 340 CAGCTG PvuII 42 CAGCTG CAG^CTG 340 CAGCTG Sau96I 27 GGCCC G^G[ACGT]CC 107 GGACC TfiI 137 GAATC G^A[AT]TC 443 GAATC XhoII 254 AGATCT [GA]^GATC[CT] 514 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### AluI BbvI BsaJI BssKI BstNI DpnII EcoRII Fnu4HI HinfI ItaI MboI MboII MnlI MvaI MwoI NdeII NlaIII PleI Sau3AI ScrFI TseI