################################## # NO CUT ENZYMES ( 195 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsiYI BslI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MslI MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tsp45I Tth111I TthHB8I Van91I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AcsI 319 AAATTC [GA]^AATT[CT] AluI 344 AGCT AG^CT AlwNI 340 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 184 CCTCAGG CC^T[ACGT]AGG ApoI 319 AAATTC [GA]^AATT[CT] AspHI 37 GTGCTC G[AT]GC[AT]^C BbsI 388 GAAGAC GAAGAC(2/6) BbvI 342 GCAGC GCAGC(8/12) BfaI 313 CTAG C^TAG BmyI 37 GTGCTC G[AGT]GC[ACT]^C BpuAI 388 GAAGAC GAAGAC(2/6) BsaJI 29 CCCTGG C^C[ACGT][ACGT]GG BsiHKAI 37 GTGCTC G[AT]GC[AT]^C BsmAI 304 GAGAC GTCTC(1/5) Bsp1286I 37 GTGCTC G[AGT]GC[ACT]^C BspHI 512 TCATGA T^CATGA BsrI 207 CCAGT ACTGG(1/-1) BstF5I 300 GGATG GGATG(2/0) BstYI 517 AGATCC [GA]^GATC[CT] Bsu36I 184 CCTCAGG CC^T[ACGT]AGG Cfr13I 27 GGCCC G^G[ACGT]CC EarI 268 CTCTTC CTCTTC(1/4) Eco81I 184 CCTCAGG CC^T[ACGT]AGG Fnu4HI 342 GCAGC GC^[ACGT]GC FokI 300 GGATG GGATG(9/13) HphI 449 TCACC GGTGA(8/7) ItaI 342 GCAGC GC^[ACGT]GC Ksp632I 268 CTCTTC CTCTTC(1/4) MaeI 313 CTAG C^TAG MaeIII 98 GTAAC ^GT[ACGT]AC MflI 517 AGATCC [GA]^GATC[CT] MseI 553 TTAA T^TAA MspA1I 343 CAGCTG C[AC]G^C[GT]G PvuII 343 CAGCTG CAG^CTG RcaI 512 TCATGA T^CATGA Sau96I 27 GGCCC G^G[ACGT]CC SfaNI 369 GATGC GCATC(5/9) SmlI 221 CTCAAG C^T[CT][GA]AG Tru9I 553 TTAA T^TAA TseI 342 GCAGC G^C[AT]GC Tsp509I 320 AATT ^AATT TspRI 327 CACTG CA[GC]TG[ACGT][ACGT]^ XbaI 312 TCTAGA T^CTAGA XhoII 517 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### AlwI 249 GATCC GGATC(4/5) 518 GATCC BseRI 395 CTCCTC GAGGAG(10/8) 190 GAGGAG BssKI 30 CCTGG ^CC[ACGT]GG 354 CCTGG BstNI 30 CCTGG CC^[AT]GG 354 CCTGG EcoRII 30 CCTGG ^CC[AT]GG 354 CCTGG HaeIII 3 GGCC GG^CC 27 GGCC MvaI 30 CCTGG CC^[AT]GG 354 CCTGG PleI 283 GACTC GAGTC(4/5) 412 GACTC ScrFI 30 CCTGG CC^[ACGT]GG 354 CCTGG TfiI 137 GAATC G^A[AT]TC 446 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 9 enzymes) # ######################################### DdeI DpnII HinfI MboI MboII MnlI NdeII NlaIII Sau3AI