################################## # NO CUT ENZYMES ( 185 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaII AviII AvrII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BpmI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI KspI MaeII MaeIII MamI MfeI MluI MroI MslI MspA1I MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tsp45I Tth111I TthHB8I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AcsI 319 AAATTT [GA]^AATT[CT] AluI 43 AGCT AG^CT AlwNI 340 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 319 AAATTT [GA]^AATT[CT] Asp700I 432 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 37 GTGCTC G[AT]GC[AT]^C AvaI 184 CCCGAG C^[CT]CG[GA]G BalI 201 TGGCCA TGG^CCA BbsI 216 GAAGAC GAAGAC(2/6) BbvI 292 GCTGC GCAGC(8/12) BfaI 313 CTAG C^TAG BlpI 39 GCTCAGC GC^T[ACGT]AGC BmyI 37 GTGCTC G[AGT]GC[ACT]^C Bpu1102I 39 GCTCAGC GC^T[ACGT]AGC BpuAI 216 GAAGAC GAAGAC(2/6) BsiHKAI 37 GTGCTC G[AT]GC[AT]^C BsmAI 391 GAGAC GTCTC(1/5) BsoBI 184 CCCGAG C^[CT]CG[GA]G Bsp1286I 37 GTGCTC G[AGT]GC[ACT]^C BspHI 512 TCATGA T^CATGA BsrI 207 CCAGT ACTGG(1/-1) BstF5I 417 CATCC GGATG(2/0) BstYI 517 AGATCC [GA]^GATC[CT] CelII 39 GCTCAGC GC^T[ACGT]AGC DraII 106 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoO109I 106 AGGGCCT [GA]G^G[ACGT]CC[CT] Fnu4HI 292 GCTGC GC^[ACGT]GC FokI 417 CATCC GGATG(9/13) ItaI 292 GCTGC GC^[ACGT]GC MaeI 313 CTAG C^TAG MflI 517 AGATCC [GA]^GATC[CT] MluNI 201 TGGCCA TGG^CCA MscI 201 TGGCCA TGG^CCA MseI 553 TTAA T^TAA NspI 361 GCATGT [GA]CATG^[CT] PflMI 87 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 512 TCATGA T^CATGA SmlI 221 CTCAAG C^T[CT][GA]AG Tru9I 553 TTAA T^TAA TseI 292 GCTGC G^C[AT]GC Tsp509I 320 AATT ^AATT TspRI 327 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 87 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 53 CCATCTGTTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 517 AGATCC [GA]^GATC[CT] XmnI 432 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### AlwI 249 GATCC GGATC(4/5) 518 GATCC BseRI 187 GAGGAG GAGGAG(10/8) 190 GAGGAG BsiYI 87 CCACAGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 183 CCCCGAGGAGG BslI 87 CCACAGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 183 CCCCGAGGAGG Cfr13I 3 GGCCC G^G[ACGT]CC 107 GGGCC DdeI 40 CTCAG C^T[ACGT]AG 80 CTCAG EaeI 26 TGGCCG [CT]^GGCC[GA] 201 TGGCCA EarI 268 CTCTTC CTCTTC(1/4) 388 GAAGAG Ksp632I 268 CTCTTC CTCTTC(1/4) 388 GAAGAG Sau96I 3 GGCCC G^G[ACGT]CC 107 GGGCC ######################################### # 3 OR MORE CUT ENZYMES ( 17 enzymes) # ######################################### BsaJI BssKI BstNI DpnII EcoRII HaeIII HinfI MboI MboII MnlI MvaI NdeII NlaIII PleI Sau3AI ScrFI TfiI