(Circular) (Six-base) MAPSORT of: pFN21AE2324.seq Check: 6467 from: 1 to: 5704 pFN21AE2324 5704 bp With 182 enzymes: SgfI * March 29, 2011 18:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3514 278 Size: 53 83 186 936 1978 2468 Fragments arranged by size: 2468 1978 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2079 3047 1937 Size: 142 968 4594 Fragments arranged by size: 4594 968 142 Acc65I G'GTAC_C Cuts at: 3025 3025 Size: 5704 AclI AA'CG_TT Cuts at: 1563 3831 4204 1563 Size: 2268 373 3063 Fragments arranged by size: 3063 2268 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2604 2661 2850 3767 5151 5419 1632 Size: 972 57 189 917 1384 268 1917 Fragments arranged by size: 1917 1384 972 917 268 189 57 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5507 AflIII A'CryG_T Cuts at: 1203 2990 4603 5580 1203 Size: 1787 1613 977 1327 Fragments arranged by size: 1787 1613 1327 977 AgeI A'CCGG_T Cuts at: 4535 4535 Size: 5704 AhdI GACnn_n'nnGTC Cuts at: 4433 4433 Size: 5704 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5704 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5704 AlwNI CAG_nnn'CTG Cuts at: 2516 3447 5019 2516 Size: 931 1572 3201 Fragments arranged by size: 3201 1572 931 ApaLI G'TGCA_C Cuts at: 3761 4917 3761 Size: 1156 4548 ApoI r'AATT_y Cuts at: 1493 2276 2791 3013 3239 3391 5500 1493 Size: 783 515 222 226 152 2109 1697 Fragments arranged by size: 2109 1697 783 515 226 222 152 AseI AT'TA_AT Cuts at: 160 1032 4258 160 Size: 872 3226 1606 Fragments arranged by size: 3226 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5704 AvaI C'yCGr_G Cuts at: 1870 1943 2073 3029 1870 Size: 73 130 956 4545 Fragments arranged by size: 4545 956 130 73 BamHI G'GATC_C Cuts at: 3034 3401 3034 Size: 367 5337 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3025 4480 618 Size: 325 217 627 1238 1455 1842 Fragments arranged by size: 1842 1455 1238 627 325 217 BanII G_rGCy'C Cuts at: 1391 3023 1391 Size: 1632 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4733 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3895 3929 1903 Size: 34 1958 34 3678 Fragments arranged by size: 3678 1958 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3596 4806 3596 Size: 1210 4494 BclI T'GATC_A Cuts at: 1548 2845 1548 Size: 1297 4407 BfrBI ATG'CAT Cuts at: 2315 2315 Size: 5704 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4315 136 Size: 107 122 71 631 605 2643 1525 Fragments arranged by size: 2643 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5699 5699 Size: 5704 BlpI GC'TnA_GC Cuts at: 3121 3121 Size: 5704 Bme1580I G_kGCm'C Cuts at: 1790 3765 4921 1790 Size: 1975 1156 2573 Fragments arranged by size: 2573 1975 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5704 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3435 4393 442 Size: 416 53 83 399 24 2018 958 1753 Fragments arranged by size: 2018 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5704 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2046 4364 1239 Size: 807 2318 2579 Fragments arranged by size: 2579 2318 807 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3829 4694 4992 3829 Size: 865 298 4541 Fragments arranged by size: 4541 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2022 4367 882 Size: 617 523 2345 2219 Fragments arranged by size: 2345 2219 617 523 BsaAI yAC'GTr Cuts at: 493 1204 3495 493 Size: 711 2291 2702 Fragments arranged by size: 2702 2291 711 BsaBI GATnn'nnATC Cuts at: 2262 5698 2262 Size: 3436 2268 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3511 3893 4528 275 Size: 53 83 186 936 1978 382 635 1451 Fragments arranged by size: 1978 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4137 4535 4809 4956 5367 4137 Size: 398 274 147 411 4474 Fragments arranged by size: 4474 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5479 Fragments arranged by size: 5479 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2498 1186 Size: 1312 4392 BseYI C'CCAG_C Cuts at: 4907 5403 4907 Size: 496 5208 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5131 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3154 3915 4943 1540 Size: 275 186 1153 761 1028 2301 Fragments arranged by size: 2301 1153 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2982 3765 3850 4921 2982 Size: 783 85 1071 3765 Fragments arranged by size: 3765 1071 783 85 BsmI GAATG_Cn' Cuts at: 3215 3308 3215 Size: 93 5611 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2982 3023 3765 3850 4921 1391 Size: 399 1192 41 742 85 1071 2174 Fragments arranged by size: 2174 1192 1071 742 399 85 41 BspEI T'CCGG_A Cuts at: 5367 5367 Size: 5704 BspHI T'CATG_A Cuts at: 2146 3593 5323 2146 Size: 1447 1730 2527 Fragments arranged by size: 2527 1730 1447 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2560 3059 844 Size: 1716 499 3489 Fragments arranged by size: 3489 1716 499 BsrBI CCG'CTC Cuts at: 1394 1823 3591 1394 Size: 429 1768 3507 Fragments arranged by size: 3507 1768 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2398 4199 4373 65 Size: 1183 1150 1801 174 1396 Fragments arranged by size: 1801 1396 1183 1150 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4348 4535 1700 Size: 110 2538 187 2869 Fragments arranged by size: 2869 2538 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5704 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5704 BssSI C'ACGA_G Cuts at: 3764 4776 3764 Size: 1012 4692 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5704 BstBI TT'CG_AA Cuts at: 4508 4508 Size: 5704 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5704 BstYI r'GATC_y Cuts at: 1972 3034 3401 3786 3803 5255 5341 5353 5699 Size: 1062 367 385 17 1452 86 12 346 Cuts at: 5699 1972 Size: 1977 Fragments arranged by size: 1977 1452 1062 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5152 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4965 Fragments arranged by size: 4965 683 56 BtsI GCAGTG_nn' Cuts at: 3220 4014 4034 3220 Size: 794 20 4890 Fragments arranged by size: 4890 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5704 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5669 DraI TTT'AAA Cuts at: 3008 3008 Size: 5704 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4711 809 Size: 507 3395 1802 Fragments arranged by size: 3395 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2066 3151 4040 Size: 54 1425 50 275 18 236 1085 889 Cuts at: 4040 5669 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1085 889 275 236 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3151 1537 Size: 275 1339 4090 Fragments arranged by size: 4090 1339 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2637 2703 2858 3634 1353 Size: 1284 66 155 776 3423 Fragments arranged by size: 3423 1284 776 155 66 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4291 4665 4811 387 Size: 1064 138 2702 374 146 1280 Fragments arranged by size: 2702 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2046 2604 2661 2850 3767 4364 5151 Size: 393 414 558 57 189 917 597 787 Cuts at: 5151 5419 1239 Size: 268 1524 Fragments arranged by size: 1524 917 787 597 558 414 393 268 189 57 EcoNI CCTnn'n_nnAGG Cuts at: 2541 2541 Size: 5704 EcoRI G'AATT_C Cuts at: 3013 3391 3013 Size: 378 5326 FspI TGC'GCA Cuts at: 4210 4210 Size: 5704 HaeII r_GCGC'y Cuts at: 1678 4851 1678 Size: 3173 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2019 2051 4345 4377 4419 Size: 32 135 32 447 32 2294 32 42 Cuts at: 4419 4451 1373 Size: 32 2626 Fragments arranged by size: 2626 2294 447 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2288 2416 3048 3294 669 Size: 988 281 350 128 632 246 3079 Fragments arranged by size: 3079 988 632 350 281 246 128 HindIII A'AGCT_T Cuts at: 748 748 Size: 5704 HpaI GTT'AAC Cuts at: 3294 3294 Size: 5704 KpnI G_GTAC'C Cuts at: 3029 3029 Size: 5704 MfeI C'AATT_G Cuts at: 3303 3303 Size: 5704 MluI A'CGCG_T Cuts at: 5580 5580 Size: 5704 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4818 5002 1305 Size: 3513 184 2007 Fragments arranged by size: 3513 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2068 10 Size: 54 1425 343 236 3646 Fragments arranged by size: 3646 1425 343 236 54 MslI CAynn'nnrTG Cuts at: 518 1166 3662 4021 4180 518 Size: 648 2496 359 159 2042 Fragments arranged by size: 2496 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3120 3797 4945 5190 1606 Size: 1514 677 1148 245 2120 Fragments arranged by size: 2120 1514 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5152 NdeI CA'TA_TG Cuts at: 387 387 Size: 5704 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5704 NotI GC'GGCC_GC Cuts at: 3151 3151 Size: 5704 NruI TCG'CGA Cuts at: 2013 2013 Size: 5704 NsiI A_TGCA'T Cuts at: 2317 2317 Size: 5704 NspI r_CATG'y Cuts at: 1132 1229 2994 3062 4607 1132 Size: 97 1765 68 1545 2229 Fragments arranged by size: 2229 1765 1545 97 68 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5704 PciI A'CATG_T Cuts at: 2990 4603 2990 Size: 1613 4091 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5704 PmeI GTTT'AAAC Cuts at: 3008 3008 Size: 5704 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3773 3805 5286 5318 3773 Size: 32 1481 32 4159 Fragments arranged by size: 4159 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5704 PsiI TTA'TAA Cuts at: 3274 3274 Size: 5704 PspXI vC'TCGA_Gb Cuts at: 2073 2073 Size: 5704 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2070 2102 2070 Size: 32 5672 PstI C_TGCA'G Cuts at: 830 2557 3056 830 Size: 1727 499 3478 Fragments arranged by size: 3478 1727 499 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5704 SalI G'TCGA_C Cuts at: 1936 3046 1936 Size: 1110 4594 SbfI CC_TGCA'GG Cuts at: 3056 3056 Size: 5704 ScaI AGT'ACT Cuts at: 3952 5664 3952 Size: 1712 3992 SfcI C'TryA_G Cuts at: 826 1045 2553 2559 2999 3052 4187 4868 5059 Size: 219 1508 6 440 53 1135 681 191 Cuts at: 5059 826 Size: 1471 Fragments arranged by size: 1508 1471 1135 681 440 219 191 53 6 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5704 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5704 SmaI CCC'GGG Cuts at: 1872 3031 1872 Size: 1159 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2073 3808 4709 4971 820 Size: 197 926 130 1735 901 262 1553 Fragments arranged by size: 1735 1553 926 901 262 197 130 SnaBI TAC'GTA Cuts at: 493 3495 493 Size: 3002 2702 SpeI A'CTAG_T Cuts at: 152 2348 152 Size: 2196 3508 SphI G_CATG'C Cuts at: 1132 3062 1132 Size: 1930 3774 SspI AAT'ATT Cuts at: 5 52 3628 5 Size: 47 3576 2081 Fragments arranged by size: 3576 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5704 StyI C'CwwG_G Cuts at: 513 1065 3143 513 Size: 552 2078 3074 Fragments arranged by size: 3074 2078 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2125 3731 3748 3901 4086 4531 325 Size: 1255 545 1606 17 153 185 445 1498 Fragments arranged by size: 1606 1498 1255 545 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3950 5662 96 Size: 274 80 33 51 1443 1973 1712 138 Fragments arranged by size: 1973 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5664 5696 5664 Size: 32 5672 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5704 XbaI T'CTAG_A Cuts at: 3040 3040 Size: 5704 XhoI C'TCGA_G Cuts at: 1943 2073 1943 Size: 130 5574 XmaI C'CCGG_G Cuts at: 1870 3029 1870 Size: 1159 4545 XmnI GAAnn'nnTTC Cuts at: 3833 3833 Size: 5704 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3512 276 Size: 53 83 186 936 1978 2468 Fragments arranged by size: 2468 1978 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PsrI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV PacI PfoI PmlI PpuMI PspOMI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI