################################## # NO CUT ENZYMES ( 156 enzymes) # ################################## AatII Acc65I AccIII AciI AclI AcyI AfaI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BcnI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaHI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI Bsp1286I Bsp1407I BspDI BspEI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KpnI KspI MaeII MfeI MluI MroI MspA1I MunI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI SspI SwaI Tth111I Van91I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccII 7 CGCG CG^CG AflIII 1274 ACATGT A^C[GA][CT]GT AlwI 1441 GGATC GGATC(4/5) AlwNI 506 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 1272 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 68 CTCGAG C^[CT]CG[GA]G AvaII 160 GGACC G^G[AT]CC BalI 61 TGGCCA TGG^CCA BbsI 1492 GAAGAC GAAGAC(2/6) BciVI 1061 GGATAC GTATCC(6/5) BpmI 20 CTGGAG CTGGAG(16/14) BpuAI 1492 GAAGAC GAAGAC(2/6) BsaI 23 GAGACC GGTCTC(1/5) BsaJI 1127 CCTTGG C^C[ACGT][ACGT]GG BseRI 478 GAGGAG GAGGAG(10/8) BsoBI 68 CTCGAG C^[CT]CG[GA]G BspHI 141 TCATGA T^CATGA BspLU11I 1274 ACATGT A^CATGT BspMI 546 ACCTGC ACCTGC(4/8) BsrI 1538 CCAGT ACTGG(1/-1) BssKI 19 CCTGG ^CC[ACGT]GG BstNI 19 CCTGG CC^[AT]GG BstUI 7 CGCG CG^CG BstYI 1441 GGATCT [GA]^GATC[CT] EaeI 61 TGGCCA [CT]^GGCC[GA] EcoNI 532 CCTAAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRII 19 CCTGG ^CC[AT]GG EcoT14I 1127 CCTTGG C^C[AT][AT]GG HapII 742 CCGG C^CGG HpaII 742 CCGG C^CGG MflI 1441 GGATCT [GA]^GATC[CT] MluNI 61 TGGCCA TGG^CCA MscI 61 TGGCCA TGG^CCA MspI 742 CCGG C^CGG MvaI 19 CCTGG CC^[AT]GG MvnI 7 CGCG CG^CG NruI 6 TCGCGA TCG^CGA PaeR7I 68 CTCGAG C^TCGAG PstI 548 CTGCAG CTGCA^G RcaI 141 TCATGA T^CATGA ScrFI 19 CCTGG CC^[ACGT]GG SpeI 343 ACTAGT A^CTAGT StuI 1124 AGGCCT AGG^CCT StyI 1127 CCTTGG C^C[AT][AT]GG XhoI 68 CTCGAG C^TCGAG XhoII 1441 GGATCT [GA]^GATC[CT] XmnI 1272 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AccI 73 GTAGAC GT^[AC][GT]AC 1245 GTCTAC BclI 840 TGATCA T^GATCA 1304 TGATCA BsaBI 253 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1456 GATGGTAATC BsiYI 434 CCAGAAGGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 532 CCTAAAAGAGG BslI 434 CCAGAAGGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 532 CCTAAAAGAGG BsrDI 386 GCAATG GCAATG(2/0) 1487 GCAATG EcoT22I 308 ATGCAT ATGCA^T 1116 ATGCAT FbaI 840 TGATCA T^GATCA 1304 TGATCA HincII 281 GTCAAC GT[CT]^[GA]AC 409 GTTGAC HindII 281 GTCAAC GT[CT]^[GA]AC 409 GTTGAC MamI 253 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1456 GATGGTAATC MslI 1179 CACAGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1308 CACCCAAGTG NsiI 308 ATGCAT ATGCA^T 1116 ATGCAT NspI 1065 ACATGC [GA]CATG^[CT] 1274 ACATGT SfcI 548 CTGCAG C^T[GA][CT]AG 554 CTACAG SmlI 68 CTCGAG C^T[CT][GA]AG 1451 CTCAAG TspRI 1196 CACTG CA[GC]TG[ACGT][ACGT]^ 1539 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AcsI AluI ApoI BbvI BfaI BsmAI BstF5I Cac8I Cfr13I DdeI DpnII EarI Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI NdeII NlaIII PleI Sau3AI Sau96I SfaNI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TthHB8I