(Circular) (Six-base) MAPSORT of: pFN21AB9945.seq Check: 8691 from: 1 to: 6295 pFN21AB9945 6295 bp With 182 enzymes: SgfI * April 1, 2011 16:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4105 278 Size: 53 83 186 936 2569 2468 Fragments arranged by size: 2569 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2079 3251 3638 1937 Size: 142 1172 387 4594 Fragments arranged by size: 4594 1172 387 142 Acc65I G'GTAC_C Cuts at: 3616 3616 Size: 6295 AclI AA'CG_TT Cuts at: 1563 3594 4422 4795 1563 Size: 2031 828 373 3063 Fragments arranged by size: 3063 2031 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2604 2661 2850 3137 3593 4358 5742 6010 Size: 972 57 189 287 456 765 1384 268 Cuts at: 6010 1632 Size: 1917 Fragments arranged by size: 1917 1384 972 765 456 287 268 189 57 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6098 AflIII A'CryG_T Cuts at: 1203 3279 5194 6171 1203 Size: 2076 1915 977 1327 Fragments arranged by size: 2076 1915 1327 977 AgeI A'CCGG_T Cuts at: 5126 5126 Size: 6295 AhdI GACnn_n'nnGTC Cuts at: 5024 5024 Size: 6295 AleI CACnn'nnGTG Cuts at: 1166 3317 1166 Size: 2151 4144 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6295 AlwNI CAG_nnn'CTG Cuts at: 2516 4038 5610 2516 Size: 1522 1572 3201 Fragments arranged by size: 3201 1572 1522 ApaLI G'TGCA_C Cuts at: 4352 5508 4352 Size: 1156 5139 ApoI r'AATT_y Cuts at: 1493 2276 2791 3085 3604 3830 3982 6091 1493 Size: 783 515 294 519 226 152 2109 1697 Fragments arranged by size: 2109 1697 783 519 515 294 226 152 AseI AT'TA_AT Cuts at: 160 1032 4849 160 Size: 872 3817 1606 Fragments arranged by size: 3817 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6295 AvaI C'yCGr_G Cuts at: 1870 1943 2073 3620 1870 Size: 73 130 1547 4545 Fragments arranged by size: 4545 1547 130 73 BamHI G'GATC_C Cuts at: 3625 3992 3625 Size: 367 5928 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3616 5071 618 Size: 325 217 627 1829 1455 1842 Fragments arranged by size: 1842 1829 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3614 1391 Size: 2223 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3504 928 Size: 971 1605 3719 Fragments arranged by size: 3719 1605 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4486 4520 1903 Size: 34 2549 34 3678 Fragments arranged by size: 3678 2549 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3060 4187 5397 3060 Size: 1127 1210 3958 Fragments arranged by size: 3958 1210 1127 BclI T'GATC_A Cuts at: 1548 2845 3309 1548 Size: 1297 464 4534 Fragments arranged by size: 4534 1297 464 BfrBI ATG'CAT Cuts at: 2315 3123 2315 Size: 808 5487 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4906 136 Size: 107 122 71 631 605 3234 1525 Fragments arranged by size: 3234 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6290 6290 Size: 6295 BlpI GC'TnA_GC Cuts at: 3712 3712 Size: 6295 Bme1580I G_kGCm'C Cuts at: 1790 4356 5512 1790 Size: 2566 1156 2573 Fragments arranged by size: 2573 2566 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6295 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4026 4984 442 Size: 416 53 83 399 24 2609 958 1753 Fragments arranged by size: 2609 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6295 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2046 4955 1239 Size: 807 2909 2579 Fragments arranged by size: 2909 2579 807 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3441 4420 5285 5583 3441 Size: 979 865 298 4153 Fragments arranged by size: 4153 979 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2022 4958 882 Size: 617 523 2936 2219 Fragments arranged by size: 2936 2219 617 523 BsaAI yAC'GTr Cuts at: 493 1204 4086 493 Size: 711 2882 2702 Fragments arranged by size: 2882 2702 711 BsaBI GATnn'nnATC Cuts at: 2262 3465 6289 2262 Size: 1203 2824 2268 Fragments arranged by size: 2824 2268 1203 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4102 4484 5119 275 Size: 53 83 186 936 2569 382 635 1451 Fragments arranged by size: 2569 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4728 5126 5400 5547 5958 4728 Size: 398 274 147 411 5065 Fragments arranged by size: 5065 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3291 3321 1178 Size: 30 165 30 1888 30 4152 Fragments arranged by size: 4152 1888 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2498 1186 Size: 1312 4983 BseYI C'CCAG_C Cuts at: 5498 5994 5498 Size: 496 5799 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5722 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3745 4506 5534 1540 Size: 275 186 1744 761 1028 2301 Fragments arranged by size: 2301 1744 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4356 4441 5512 4356 Size: 85 1071 5139 Fragments arranged by size: 5139 1071 85 BsmI GAATG_Cn' Cuts at: 3806 3899 3806 Size: 93 6202 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3614 4356 4441 5512 1391 Size: 399 1824 742 85 1071 2174 Fragments arranged by size: 2174 1824 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5958 5958 Size: 6295 BspHI T'CATG_A Cuts at: 2146 4184 5914 2146 Size: 2038 1730 2527 Fragments arranged by size: 2527 2038 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2560 3650 844 Size: 1716 1090 3489 Fragments arranged by size: 3489 1716 1090 BsrBI CCG'CTC Cuts at: 1394 1823 4182 1394 Size: 429 2359 3507 Fragments arranged by size: 3507 2359 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2398 3499 4790 4964 65 Size: 1183 1150 1101 1291 174 1396 Fragments arranged by size: 1396 1291 1183 1150 1101 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4939 5126 1700 Size: 110 3129 187 2869 Fragments arranged by size: 3129 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6295 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6295 BssSI C'ACGA_G Cuts at: 4355 5367 4355 Size: 1012 5283 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6295 BstBI TT'CG_AA Cuts at: 5099 5099 Size: 6295 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6295 BstYI r'GATC_y Cuts at: 1972 3446 3625 3992 4377 4394 5846 5932 5944 Size: 1474 179 367 385 17 1452 86 12 Cuts at: 5944 6290 1972 Size: 346 1977 Fragments arranged by size: 1977 1474 1452 385 367 346 179 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5743 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5556 Fragments arranged by size: 5556 683 56 BtsI GCAGTG_nn' Cuts at: 3811 4605 4625 3811 Size: 794 20 5481 Fragments arranged by size: 5481 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6295 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6260 DraI TTT'AAA Cuts at: 3599 3599 Size: 6295 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5302 809 Size: 507 3986 1802 Fragments arranged by size: 3986 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2066 3742 4631 Size: 54 1425 50 275 18 236 1676 889 Cuts at: 4631 6260 8 Size: 1629 43 Fragments arranged by size: 1676 1629 1425 889 275 236 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3742 1537 Size: 275 1930 4090 Fragments arranged by size: 4090 1930 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2637 2703 2858 3543 4225 1353 Size: 1284 66 155 685 682 3423 Fragments arranged by size: 3423 1284 685 682 155 66 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4882 5256 5402 387 Size: 1064 138 3293 374 146 1280 Fragments arranged by size: 3293 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2046 2604 2661 2850 3137 3593 4358 Size: 393 414 558 57 189 287 456 765 Cuts at: 4358 4955 5742 6010 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 765 597 558 456 414 393 287 268 189 57 EcoNI CCTnn'n_nnAGG Cuts at: 2541 2541 Size: 6295 EcoRI G'AATT_C Cuts at: 3604 3982 3604 Size: 378 5917 FspI TGC'GCA Cuts at: 4801 4801 Size: 6295 HaeII r_GCGC'y Cuts at: 1678 5442 1678 Size: 3764 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2019 2051 4936 4968 5010 Size: 32 135 32 447 32 2885 32 42 Cuts at: 5010 5042 1373 Size: 32 2626 Fragments arranged by size: 2885 2626 447 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2288 2416 3639 3885 669 Size: 988 281 350 128 1223 246 3079 Fragments arranged by size: 3079 1223 988 350 281 246 128 HindIII A'AGCT_T Cuts at: 748 748 Size: 6295 HpaI GTT'AAC Cuts at: 3885 3885 Size: 6295 KpnI G_GTAC'C Cuts at: 3620 3620 Size: 6295 MfeI C'AATT_G Cuts at: 3894 3894 Size: 6295 MluI A'CGCG_T Cuts at: 6171 6171 Size: 6295 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5409 5593 1305 Size: 4104 184 2007 Fragments arranged by size: 4104 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2068 10 Size: 54 1425 343 236 4237 Fragments arranged by size: 4237 1425 343 236 54 MslI CAynn'nnrTG Cuts at: 518 1166 3188 3317 4253 4612 4771 518 Size: 648 2022 129 936 359 159 2042 Fragments arranged by size: 2042 2022 936 648 359 159 129 MspA1I CmG'CkG Cuts at: 1606 3711 4388 5536 5781 1606 Size: 2105 677 1148 245 2120 Fragments arranged by size: 2120 2105 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5743 NdeI CA'TA_TG Cuts at: 387 387 Size: 6295 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6295 NotI GC'GGCC_GC Cuts at: 3742 3742 Size: 6295 NruI TCG'CGA Cuts at: 2013 2013 Size: 6295 NsiI A_TGCA'T Cuts at: 2317 3125 2317 Size: 808 5487 NspI r_CATG'y Cuts at: 1132 1229 3074 3283 3653 5198 1132 Size: 97 1845 209 370 1545 2229 Fragments arranged by size: 2229 1845 1545 370 209 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6295 PciI A'CATG_T Cuts at: 3279 5194 3279 Size: 1915 4380 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6295 PmeI GTTT'AAAC Cuts at: 3599 3599 Size: 6295 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4364 4396 5877 5909 4364 Size: 32 1481 32 4750 Fragments arranged by size: 4750 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6295 PsiI TTA'TAA Cuts at: 3865 3865 Size: 6295 PspXI vC'TCGA_Gb Cuts at: 2073 2073 Size: 6295 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2070 2102 2070 Size: 32 6263 PstI C_TGCA'G Cuts at: 830 2557 3647 830 Size: 1727 1090 3478 Fragments arranged by size: 3478 1727 1090 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6295 SalI G'TCGA_C Cuts at: 1936 3637 1936 Size: 1701 4594 SbfI CC_TGCA'GG Cuts at: 3647 3647 Size: 6295 ScaI AGT'ACT Cuts at: 4543 6255 4543 Size: 1712 4583 SfcI C'TryA_G Cuts at: 826 1045 2553 2559 3643 4778 5459 5650 826 Size: 219 1508 6 1084 1135 681 191 1471 Fragments arranged by size: 1508 1471 1135 1084 681 219 191 6 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6295 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6295 SmaI CCC'GGG Cuts at: 1872 3622 1872 Size: 1750 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2073 3456 4399 5300 5562 820 Size: 197 926 130 1383 943 901 262 1553 Fragments arranged by size: 1553 1383 943 926 901 262 197 130 SnaBI TAC'GTA Cuts at: 493 4086 493 Size: 3593 2702 SpeI A'CTAG_T Cuts at: 152 2348 152 Size: 2196 4099 SphI G_CATG'C Cuts at: 1132 3653 1132 Size: 2521 3774 SspI AAT'ATT Cuts at: 5 52 4219 5 Size: 47 4167 2081 Fragments arranged by size: 4167 2081 47 StuI AGG'CCT Cuts at: 1517 3131 1517 Size: 1614 4681 StyI C'CwwG_G Cuts at: 513 1065 3132 3734 513 Size: 552 2067 602 3074 Fragments arranged by size: 3074 2067 602 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2125 3329 4322 4339 4492 4677 5122 Size: 1255 545 1204 993 17 153 185 445 Cuts at: 5122 325 Size: 1498 Fragments arranged by size: 1498 1255 1204 993 545 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4541 6253 96 Size: 274 80 33 51 1443 2564 1712 138 Fragments arranged by size: 2564 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3291 3323 6255 6287 3291 Size: 32 2932 32 3299 Fragments arranged by size: 3299 2932 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6295 XbaI T'CTAG_A Cuts at: 3631 3631 Size: 6295 XhoI C'TCGA_G Cuts at: 1943 2073 1943 Size: 130 6165 XmaI C'CCGG_G Cuts at: 1870 3620 1870 Size: 1750 4545 XmnI GAAnn'nnTTC Cuts at: 3281 4424 3281 Size: 1143 5152 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4103 276 Size: 53 83 186 936 2569 2468 Fragments arranged by size: 2569 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PsrI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV PacI PfoI PmlI PpuMI PspOMI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI