################################## # NO CUT ENZYMES ( 180 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRI EcoRV EcoT14I EcoT22I FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaI HpaII KasI KpnI KspI MaeII MfeI MflI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StyI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AciI 141 CCGC CCGC(-3/-1) AlwI 703 GATCC GGATC(4/5) AvaII 300 GGTCC G^G[AT]CC BanII 610 GAGCTC G[GA]GC[CT]^C BbsI 442 GAAGAC GAAGAC(2/6) BbvI 247 GCTGC GCAGC(8/12) BclI 390 TGATCA T^GATCA BfaI 314 CTAG C^TAG BpmI 707 CTGGAG CTGGAG(16/14) BpuAI 442 GAAGAC GAAGAC(2/6) BsaBI 730 GATAATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmAI 495 GTCTC GTCTC(1/5) BsmI 691 GAATGC GAATGC(1/-1) BspHI 324 TCATGA T^CATGA BsrBI 141 CCGCTC CCGCTC(-3/-3) BstEII 549 GGTAACC G^GT[ACGT]ACC BstF5I 417 GGATG GGATG(2/0) BstPI 549 GGTAACC G^GT[ACGT]ACC Cfr13I 300 GGTCC G^G[ACGT]CC DraIII 601 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 759 CTCTTC CTCTTC(1/4) EcoO65I 549 GGTAACC G^GT[ACGT]ACC FbaI 390 TGATCA T^GATCA Fnu4HI 247 GCTGC GC^[ACGT]GC FokI 417 GGATG GGATG(9/13) HincII 254 GTCAAC GT[CT]^[GA]AC HindII 254 GTCAAC GT[CT]^[GA]AC ItaI 247 GCTGC GC^[ACGT]GC Ksp632I 759 CTCTTC CTCTTC(1/4) MaeI 314 CTAG C^TAG MamI 730 GATAATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MwoI 61 GCAAGCAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NlaIV 299 GGGTCC GG[ACGT]^[ACGT]CC NspI 582 GCATGC [GA]CATG^[CT] RcaI 324 TCATGA T^CATGA SacI 610 GAGCTC GAGCT^C Sau96I 300 GGTCC G^G[ACGT]CC SphI 582 GCATGC GCATG^C TseI 247 GCTGC G^C[AT]GC Tsp45I 521 GTGAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccI 264 GTCTAC GT^[AC][GT]AC 754 GTAGAC AspHI 317 GAGCAC G[AT]GC[AT]^C 610 GAGCTC BmyI 317 GAGCAC G[AGT]GC[ACT]^C 610 GAGCTC BsiHKAI 317 GAGCAC G[AT]GC[AT]^C 610 GAGCTC BsiYI 96 CCAACAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 191 CCACCAAAAGG BslI 96 CCAACAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 191 CCACCAAAAGG Bsp1286I 317 GAGCAC G[AGT]GC[ACT]^C 610 GAGCTC BssKI 642 CCAGG ^CC[ACGT]GG 706 CCTGG BstNI 642 CCAGG CC^[AT]GG 706 CCTGG Cac8I 61 GCAAGC GC[ACGT]^[ACGT]GC 582 GCATGC DpnII 391 GATC ^GATC 703 GATC EcoRII 642 CCAGG ^CC[AT]GG 706 CCTGG HphI 520 GGTGA GGTGA(8/7) 349 TCACC MboI 391 GATC ^GATC 703 GATC MslI 616 CATAAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 686 CATTTGAATG MvaI 642 CCAGG CC^[AT]GG 706 CCTGG NdeII 391 GATC ^GATC 703 GATC Sau3AI 391 GATC ^GATC 703 GATC ScrFI 642 CCAGG CC^[ACGT]GG 706 CCTGG TfiI 328 GATTC G^A[AT]TC 514 GAATC TspRI 206 CACTG CA[GC]TG[ACGT][ACGT]^ 632 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 17 enzymes) # ######################################### AcsI AfaI AluI ApoI DdeI HaeIII HinfI MaeIII MboII MnlI MseI NlaIII PleI RsaI StuI Tru9I Tsp509I