################################## # NO CUT ENZYMES ( 162 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BbvI BciVI BclI BfaI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI Bsp1286I BspDI BspEI BspLU11I BsrBI BsrFI BssHII Bst1107I BstAPI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI MaeI MamI MfeI MflI MluI MluNI MroI MscI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI TfiI TseI Tth111I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AccI 291 GTCTAC GT^[AC][GT]AC AccII 5 CGCG CG^CG AflII 471 CTTAAG C^TTAAG AluI 92 AGCT AG^CT AvaI 266 CCCGGG C^[CT]CG[GA]G BbsI 575 GTCTTC GAAGAC(2/6) BcgI 196 GCACAGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 471 CTTAAG C^TTAAG BpmI 147 CTGGAG CTGGAG(16/14) BpuAI 575 GTCTTC GAAGAC(2/6) BsmFI 21 GTCCC GGGAC(10/14) BsmI 429 GAATGC GAATGC(1/-1) BsoBI 266 CCCGGG C^[CT]CG[GA]G Bsp1407I 547 TGTACA T^GTACA BspHI 423 TCATGA T^CATGA BspMI 101 GCAGGT ACCTGC(4/8) BsrDI 567 CATTGC GCAATG(2/0) BsrGI 547 TGTACA T^GTACA BssSI 59 CTCGTG CACGAG(-5/-1) BstBI 238 TTCGAA TT^CGAA BstNI 86 CCAGG CC^[AT]GG BstUI 5 CGCG CG^CG Cac8I 245 GCCCGC GC[ACGT]^[ACGT]GC Cfr13I 264 GGCCC G^G[ACGT]CC DpnII 614 GATC ^GATC DraI 592 TTTAAA TTT^AAA DsaI 4 CCGCGG C^C[GA][CT]GG EaeI 135 CGGCCA [CT]^GGCC[GA] EarI 272 GAAGAG CTCTTC(1/4) EcoRII 86 CCAGG ^CC[AT]GG EcoT14I 372 CCTTGG C^C[AT][AT]GG FauI 246 CCCGC CCCGC(4/6) HinfI 38 GACTC G^A[ACGT]TC Ksp632I 272 GAAGAG CTCTTC(1/4) KspI 4 CCGCGG CCGC^GG MaeII 450 ACGT A^CGT MboI 614 GATC ^GATC MslI 424 CATGAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 4 CCGCGG C[AC]G^C[GT]G MvaI 86 CCAGG CC^[AT]GG MvnI 5 CGCG CG^CG NdeII 614 GATC ^GATC NspI 571 GCATGT [GA]CATG^[CT] NspV 238 TTCGAA TT^CGAA PleI 38 GACTC GAGTC(4/5) RcaI 423 TCATGA T^CATGA SacII 4 CCGCGG CCGC^GG Sau3AI 614 GATC ^GATC Sau96I 264 GGCCC G^G[ACGT]CC SfaNI 492 GCATC GCATC(5/9) SfcI 222 CTACAG C^T[GA][CT]AG SfuI 238 TTCGAA TT^CGAA SmaI 266 CCCGGG CCC^GGG SmlI 471 CTTAAG C^T[CT][GA]AG SspBI 547 TGTACA T^GTACA StuI 200 AGGCCT AGG^CCT StyI 372 CCTTGG C^C[AT][AT]GG Tsp45I 333 GTCAC ^GT[GC]AC XmaCI 266 CCCGGG C^CCGGG XmaI 266 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AcsI 388 GAATTT [GA]^AATT[CT] 436 AAATTT AfaI 105 GTAC GT^AC 548 GTAC ApoI 388 GAATTT [GA]^AATT[CT] 436 AAATTT BseRI 10 CTCCTC GAGGAG(10/8) 166 GAGGAG BstF5I 496 CATCC GGATG(2/0) 544 GGATG FokI 496 CATCC GGATG(9/13) 544 GGATG HapII 262 CCGG C^CGG 267 CCGG HpaII 262 CCGG C^CGG 267 CCGG HphI 111 GGTGA GGTGA(8/7) 367 TCACC MaeIII 333 GTCAC ^GT[ACGT]AC 625 GTAAC MspI 262 CCGG C^CGG 267 CCGG MwoI 71 GCGACATCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 256 GCTTCCCCGGC NlaIV 8 GGCTCC GG[ACGT]^[ACGT]CC 149 GGAGCC RsaI 105 GTAC GT^AC 548 GTAC TspRI 81 CAGTG CA[GC]TG[ACGT][ACGT]^ 287 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### AciI BcnI BsaJI BsiYI BslI BsrI BssKI DdeI Fnu4HI HaeIII ItaI MboII MnlI MseI NciI NlaIII ScrFI TaqI Tru9I Tsp509I TthHB8I